The invention provides a method for identifying
red bean varieties by utilizing
genome SSR and EST-SSR fingerprints and application and belongs to the technical field of
plant variety identification. A mark combination composed of 5 pairs of
genome SSRs and 5 pairs of EST-SSR primers is provided,
nucleotide sequences of the 5 pairs of
genome SSRs and the 5 pairs of EST-SSR primers are respectively as shown in SEQ ID No. 1-20, and fingerprints of 32 kinds of red beans are constructed; according to the fingerprints,
red bean seed variety identification and
genetic diversity evaluation work can be finished within 4 hours by adopting the mark combination, and the method has the advantages of being efficient, accurate, low in cost and simple and convenient to operate. According to the method,
red bean seed authenticity is effectively monitored, variety heritable variation and genetic relationships of varieties are disclosed from the
DNA level,
crop varieties are protected, forged and fake commodity varieties are prevented from entering the market,
technical support is provided for reasonable utilization of excellent
germ plasm in the red bean variety breeding process, and the method has good application prospect.