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Method for screening specific molecular markers of rice fertility restorer genes

A fertility restoration gene and molecular marker technology, which can be applied to biochemical equipment and methods, and the determination/inspection of microorganisms, etc., can solve problems such as increasing the difficulty of breeding work and increasing workload.

Inactive Publication Date: 2010-06-02
CHINA NAT RICE RES INST
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Problems solved by technology

On the basis of the location of the fertility restorer gene, molecular markers that are closely linked to the restorer gene can be screened, but the problem of combination specificity of marker polymorphisms is often encountered, that is, markers that are polymorphic between specific parents will not be used in breeding. There may be monomorphism between the parents of the population, and it is necessary to re-screen the polymorphic markers for the parents of the specific breeding population, which not only increases the workload, but also increases the difficulty of breeding work

Method used

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  • Method for screening specific molecular markers of rice fertility restorer genes
  • Method for screening specific molecular markers of rice fertility restorer genes
  • Method for screening specific molecular markers of rice fertility restorer genes

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[0009] specific implementation plan

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Abstract

The invention provides a method for screening specific molecular markers of rice fertility restorer genes, which is characterized by applying rice germ plasm resources carrying and not carrying fertility restorer genes and screening the molecular markers, the sterile lines and (or) the restorer lines of which are in specific banding patterns, from the molecular markers closely linked with the rice fertility restorer genes Rf3 and Rf4. With the markers adopted to carry out auxiliary selection on the molecular markers of rice fertility restorer genes, the breeding efficiency of the sterile lines and the restorer lines of indica type rice can be improved.

Description

technical field [0001] The invention relates to a method for screening specific molecular markers using rice germplasm resources, in particular to a method for screening rice fertility restoration gene specific molecular markers using rice germplasm resources. Background technique [0002] Rice cytoplasmic male sterility (CMS) and its fertility recovery are the basis for realizing three-line matching of rice. Among many CMSs, the wild type is the earliest and most widely used sterile cytoplasmic type in the three-line hybrid rice in my country, and it is different from other cytoplasmic types (K type, dwarf type, D type, Gang type and Indonesian paddy valley type) have similar recovery relations. This type of cytoplasmic fertility recovery is mainly controlled by two major genes, Rf3 located on the short arm of chromosome 1 and Rf4 located on the long arm of chromosome 10. [0003] In breeding practice, the selection of restorer genes is usually determined by a large number...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 黄得润庄杰云元芳丽樊叶杨
Owner CHINA NAT RICE RES INST
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