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Screening method of Muricidae mitochondrion COI gene amplification primer

An amplification primer and gene amplification technology, which is applied in the field of screening of mitochondrial COI gene amplification primers of Ospiraceae, can solve the problems of hindering the use of molecular technology, large sequence differences, low amplification efficiency of COI universal primers, etc.

Inactive Publication Date: 2011-01-12
OCEAN UNIV OF CHINA
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Problems solved by technology

[0004] However, the COI sequence variability of Mururaceae species is high, and the sequence differences of different species are large, which makes the amplification efficiency of COI universal primers low, and some species cannot be amplified at all, which hinders the use of molecular techniques

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Embodiment Construction

[0008] The screening method of the snail family mitochondrial COI gene amplification primer of the present invention adopts three steps: a, amplify the COI sequence of part of the snail family species with the COI universal amplification primer; b, amplify the COI universal Compare the primer sequences with the COI sequences of some Murexidae species that have been obtained, find out all the mutant bases of the Murexaceae mitochondrial COI sequence in the COI universal amplification primer sequence segment, and re-synthesize different Murexaceae COI amplifications Primers; c, from newly synthesized different COI amplification primers, a pair of primers with the best effect in amplifying Mururaceae species was selected.

[0009] The present invention will be further described below in conjunction with embodiment:

[0010] 1. Sample collection: From 2005 to 2010, a total of 200 individuals of 10 genera and 25 species of Murexaceae were collected from the north and south coasts o...

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Abstract

The invention discloses a screening method of a Muricidae mitochondrion COI gene amplification primer, which is characterized by comprising the following steps: a. a COI general amplification primer is used for amplifying the COI sequence of parts of Muricidae species; b. the sequence of the COI general amplification product is compared with the COI sequence of the obtained parts of Muricidae species to find out all mutation bases of the Muricidae mitochondrion COI sequence on the COI general amplification primer sequence section so as to recombine different Muricidae COI amplification primers; and c. a pair of primers with best Muricidae species amplification effect is obtained by screening the newly-combined different COI amplification primers. The Muricidae mitochondrion COI gene amplification primer of the invention can effectively amplify the COI sequence of different specimens of the Muricidae, and has an important meaning for researching the species taxonomy, system development and systematic geography of Muricidae and protecting Muricidae germ plasm resource.

Description

technical field [0001] The present invention relates to a screening method for the amplification primers of the shellfish mitochondrial COI [cytochrome c oxidase subunit I (cytochrome c oxidase subunit I)] gene, in particular to a screening method for the amplification primers of the mitochondrial COI gene of the family Murexaceae method. Background technique [0002] The family Muricidae belongs to Gastropoda, Prosobranchia, and Neogastropoda. It is distributed in seas all over the world. It is the second largest species of Neogastropoda. major. Murex species play an important role in maintaining the ecological balance of the ocean intertidal zone, and some species can also be used as indicators for the detection of marine toxic pollutants tributyltin (TBT). Some species of the genus Rapana and Thais have delicious meat and are of great economic value. However, due to the high variability in the external shell morphology and radula structure of Mururidae, it is extremely...

Claims

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Application Information

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IPC IPC(8): C12N15/10
Inventor 邹山梅李琪孔令锋
Owner OCEAN UNIV OF CHINA
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