702results about How to "Efficient amplification" patented technology

The present invention is directed to a method for isothermal amplification of a plurality of different target nucleic acids, wherein the different target nucleic acids are amplified using universal primers and colonies produced thereby can be distinguished from each other. The method, therefore, generates distinct colonies of amplified nucleic acid sequences that can be analyzed by various means to yield information particular to each distinct colony.

The present invention provides methods for marking nucleic acid samples with detectable markers by adding different combinations of marker molecules to each sample. Each sample may be marked with a different combination of two or more marker molecules each carrying a different tag nucleic acid sequences. The tag nucleic acid sequences may be random sequences that are not naturally occurring in the nucleic acid sample and do not cross hybridize to sequences naturally occurring in the nucleic acid sample. Methods of detecting the combination of tag sequences present in a sample, in parallel with methods of genetic analysis of the sample are disclosed. Kits containing marker molecules suitable for generating barcoded samples by mixing different combinations of marker molecules into each sample are also disclosed.

An information device includes a controller for controlling the display position and the display size of a window, a semi-maximization instruction receiving part for receiving instructions for semi-maximizing the window, a semi-maximization region setting part for setting a plurality of display regions in which the window can be semi-maximized, and a display region determination part for determining the display region in which the window is semi-maximized. When the semi-maximization instruction receiving part receives the instructions for semi-maximizing the window, the controller allows the window to be enlarged and displayed in the entire area of the display region determined by the display region determination part from the plurality of display regions.

A system and method for semantic segmentation using hybrid dilated convolution (HDC) are disclosed. A particular embodiment includes: receiving an input image; producing a feature map from the input image; performing a convolution operation on the feature map and producing multiple convolution layers; grouping the multiple convolution layers into a plurality of groups; applying different dilation rates for different convolution layers in a single group of the plurality of groups; and applying a same dilation rate setting across all groups of the plurality of groups.

The invention provides a method for detecting and quantifying the amount of target molecules, such as nucleic acids or proteins in a sample. The target molecules are first recognized and bounded by target-specific probes, generally nucleic acids or proteins that bind specifically to the targets, each of which is labeled with a short single-stranded nucleic acid probe, either DNA or RNA, with distinct molecular weight. This label is called an oligonucleotide mass tag. One or several standard oligonucleotide sequences can be designed with similar sequence but distinct molecular weight to those oligonucleotide mass tags. Then the oligonucleotide mass tags associated with bounded probes and the standard sequences are co-amplified using a pair of common primers. The presence and / or amount of each oligonucleotide mass tag, which corresponds to the amount of corresponding target molecule, is determined by a primer extension reaction and quantification of the primer extension product.

This disclosure provides platforms for delivery of herpes virus proteins to cells, particularly proteins that form complexes in vivo. In some embodiments these proteins and the complexes they form elicit potent neutralizing antibodies. Thus, presentation of herpes virus proteins using the disclosed platforms permits the generation of broad and potent immune responses useful for vaccine development.

The present invention provides methods kits and systems for performing multiple displacement amplification reactions. In one method a sample of nucleic acid is provided. The nucleic acid is contacted with a reaction mixture which includes a set of oligonucleotide primers, a one or more polymerase enzymes and a detergent. The reaction mixture is then subjected to conditions under which the nucleic acid sequence is amplified to produce an amplified product in a multiple displacement reaction. The method may also be carried out by contacting the nucleic acid with the reaction mixture in the form of an emulsion. A kit is also provided for carrying out either the methods described above. The kit includes one or more polymerases, a plurality of primers and a detergent. The kit may also include a hydrophobic polymer and may include instructions for performing a multiple displacement amplification reaction on a nucleic acid sample.

The presently claimed invention provides methods, compositions, and apparatus for analyzing nucleic acids isolated from blood. Specifically, the present invention provides a method of analyzing blood samples by blocking amplification of selected unwanted RNAs and subsequently analyzing the amplified sample by hybridization to a plurality of probes attached to a solid support. In one embodiment, the invention provides enriching for a population of interest in a complex population by diminishing the presence of an unwanted sequence that may interfere with the analysis of sequences of interest.

A power amplification apparatus and method provide for controlling envelope modulation of a Radio Frequency (RF) signal. The power amplification apparatus includes a linear amplifier configured to receive an input signal to be amplified, and generate a linear output signal for compensating for a current ripple of an amplified signal and a switch control signal having a current obtained by dividing the linear output signal by a predetermined ratio. The power amplification apparatus also includes a switching amplifier configured to receive the switch control signal through a multi-mode resistor having a variable resistance, and generate the amplified signal. The variable resistance of the multi-mode resistor determines a switching frequency representing an operating speed of the switching amplifier, and is adjusted according to a communication mode of the input signal.

A power amplifier (1) for receiving and amplifying an input signal (10) and outputting an output signal (11) is disclosed. The power amplifier comprises: N power amplifying units (12) (N is an integer larger than 1) connected in parallel so as to output amplified signals in response to the input signal (10); an output combining unit (14) for combining the output signals from the N power amplifying units (12) and outputting a combined signal as the output signal (11) of the power amplifier; and an amplitude controlling unit (15) for selectively turning ON each of the N power amplifying units (12) based on an amplitude of the input signal (10). In the power amplifier, the amplitude controlling unit (15) may comprise N amplitude adjusters (113) connected in parallel for adjusting the amplitude of the input signal (110) of the power amplifier; and a controller (115) for selectively turning ON each of the N power amplifying units (112) and controlling the amplitude adjusters (113) so that an amplitude of the output signal (111) becomes a substantially continuous function with respect to the amplitude of the input signal (110). The power amplifier may further comprise a local oscillator (222) outputting an constant envelope signal, receiving a modulation signal (210) as the input signal of the power amplifier and outputting an amplified modulated signal (211) as the output signal of the power amplifier; wherein the N power amplifying units comprise N saturation amplifying units connected in parallel so as to amplify the constant envelope signal from the local oscillator; and the amplitude controlling unit comprises an amplifying controller (215) for selectively turning ON each of the N saturation amplifying units (212) based on an amplitude of the modulation signal (210).

The invention discloses a nucleic acid isothermal amplification method by polymerase spiral reaction (PSR). A pair of oligonucleotide primers is used, mutually reverse nucleotide fragments are added to 5' ends of the primers, a target gene is subjected to PSR under the action of the primers and deoxyribonucleic acid (DNA) polymerase and under the isothermal condition, self-spiral extending of the target gene can be achieved, and thereby, nucleic acid amplification can be finished. According to the nucleic acid isothermal amplification method by PSR, a novel technology platform is provided for nucleic acid detection, amplification of a product is simple, an amplification product is applicable to the field of detection and can be slightly processed to be subjected to clone recycling and sequencing, the method can be applied to all fields which require nucleic acid amplification, the market prospect is wide, the economic and social benefits are large, and the method is suitable for large-range popularization and application.

p-MOSFETs (21) and (22) are used as amplifying elements for amplifying a signal input from a loop antenna (1) and are directly connected to the loop antenna (1). Thus, the signal input from the loop antenna (1) can be received in a high impedance through the p-MOSFETs (21) and (22). Consequently, a transformer for carrying out a conversion into a high impedance or the like is not required, and furthermore, the impedance of the loop antenna 1 itself does not need to be increased, thereby suppressing the occurrence of a current noise.

In a transmission power amplifier apparatus, a signal calculator divides an input signal into a plurality of N constant amplitude signals having a substantially identical predetermined constant amplitude value and having predetermined phases different from each other, and N power amplifiers amplify electric powers of the N constant amplitude signals under same predetermined operating condition. A power combiner combines the electric powers of the N power-amplified constant amplitude signals, and outputs a combined output signal. A controller controls the signal calculator to detect an amplitude value of the input signal, to detect an average value and one of a maximum value of the amplitude value of the input signal and a peak-to-peak value of the amplitude value for a unit time interval based on the detected amplitude value of the input signal, and to divide the input signal into the N constant amplitude signals having a constant amplitude value and different phases.

A system and method for semantic segmentation using hybrid dilated convolution (HDC) are disclosed. A particular embodiment includes: receiving an input image; producing a feature map from the input image; performing a convolution operation on the feature map and producing multiple convolution layers; grouping the multiple convolution layers into a plurality of groups; applying different dilation rates for different convolution layers in a single group of the plurality of groups; and applying a same dilation rate setting across all groups of the plurality of groups.

A magnetocaloric effect heterostructure having a core layer of a magnetostructural material with a giant magnetocaloric effect having a magnetic transition temperature equal to or greater than 150 K, and a constricting material layer coated on at least one surface of the magnetocaloric material core layer. The constricting material layer may enhance the magnetocaloric effect by restriction of volume changes of the core layer during application of a magnetic field to the heterostructure. A magnetocaloric effect heterostructure powder comprising a plurality of core particles of a magnetostructural material with a giant magnetocaloric effect having a magnetic transition temperature equal to or greater than 150 K, wherein each of the core particles is encapsulated within a coating of a constricting material is also disclosed. A method for enhancing the magnetocaloric effect within a giant magnetocaloric material including the step of coating a surface of the magnetocaloric material with a constricting material is disclosed.

A magnetocaloric effect heterostructure having a core layer of a magnetostructural material with a giant magnetocaloric effect having a magnetic transition temperature equal to or greater than 150 K, and a constricting material layer coated on at least one surface of the magnetocaloric material core layer. The constricting material layer may enhance the magnetocaloric effect by restriction of volume changes of the core layer during application of a magnetic field to the heterostructure. A magnetocaloric effect heterostructure powder comprising a plurality of core particles of a magnetostructural material with a giant magnetocaloric effect having a magnetic transition temperature equal to or greater than 150 K, wherein each of the core particles is encapsulated within a coating of a constricting material is also disclosed. A method for enhancing the magnetocaloric effect within a giant magnetocaloric material including the step of coating a surface of the magnetocaloric material with a constricting material is disclosed.

A low-noise bladeless fan includes a base, a nozzle at the top of the base and noise-reduction components located within the base. The noise-reduction components include a first noise-reduction component, a second noise-reduction component and a third noise-reduction component. The first noise-reduction component is located at an interface between air outlets and an internal passage for airflow reduces noise by isolating, obstructing and separating noise created inside the outer casing. The second noise-reduction component is disposed within an impeller fixture reduces noise by absorbing noise created inside the outer casing. Through-holes are disposed in the bottom of the outer casing act as the third noise-reduction component and also reduce noise. The arrangement of noise-reduction components can reduce noise created in the fan, thereby cooling users with natural and soft wind.

A lighting module includes an illuminant unit, a light guide pillar and a first reflecting member. The illuminant unit has an optical axis and includes at least a LED. The light guide pillar includes a base and a light guide part, the LED disposed on the base, the light guide pillar is extended from the base along the optical axis and an end of the light guide pillar has a top surface. The top surface is concave toward the base and is continuous. The first reflecting member is disposed on the top surface. Wherein a partial light emitted by the illuminant unit is travelling to the first reflecting member through the light guide pillar, the first reflecting member reflects the partial light such that the partial light emits from the light guide part.

A waterproof acoustic chamber that is adapted to hold and protect a smartphone is disclosed. The chamber includes a body portion that is configured to encapsulate a smartphone, which exhibits an internal dimension that accommodates, at a user's option, a smartphone with (or without) its own protective casing. The body portion is configured to prevent water from entering an internal area of the body portion when exposed to water. The body portion includes at least one speaker area that includes a material through which audio content that is generated by the smartphone may leave the body portion. The chamber further includes a compartment having an open side that is configured to receive and attach to the backside of the body portion. The compartment includes two sides that each extend beyond a parallel side of the body portion, with the speaker area and compartment being effective to amplify and channel the audio content that is generated by the smartphone.

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

This document provides novel drills and / or reamers that are well-suited for making holes in a variety of materials including, but not limited to, metals, ceramics, glass, wood, plasterboard, plastics, stone, composites, synthetics, silicon or multi-layered or hybridized substrates. In some embodiments, these drilling devices have some centers of mass that are offset from the axis of rotation. Accordingly, such drills and / or reamers may rotate and cut using a precessional pattern of motion. Precessional cutting devices may display a mechanical wave pattern in relationship to the longitudinal axis of the device. If the cutting device is fabricated from a flexible material, for example Nickel-Titanium, bodily deflection of the device may result during rotation.

The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. In the process according to the present invention, a primer comprising in its 3′-end portion a sequence (Ac′) which hybridizes a sequence (A) in the 3′-end portion of the target nucleic acid sequence, and in the 5′-side of said sequence (Ac′) a sequence (B′) which hybridizes the complementary sequence (Bc) of a sequence (B) positioned in the 5′-side of said sequence (A) on the target nucleic acid sequence, wherein {X−(Y−Y′)} / X is in the range of −1.00 to 1.00, in which X denotes the number of bases in said sequence (Ac′), Y denotes the number of bases in the region flanked by said sequences (A) and (B) in the target nucleic acid sequence, and Y′ denotes the number of bases in an intervening sequence between said sequences (Ac′) and (B′) (Y′ may be zero).

An automatic ball launcher for pets includes a body shell, a ball launch aisle, a ball launch mechanism which is connected to the ball launch aisle and near the outlet for the ball; the ball launch mechanism comprises one or more ball launch wheels and one or more ball launch motors; the ball launch wheels are set on two sides of the ball launch aisle, a face of which is a concaved curve; the concaved curve matches the outline of the ball; regular teeth are arranged on the concaved curve; the ball launch wheels are driven by one or more ball launch motor and launch the ball from the outlet. The present invention effectively enlarged the contact surface between the wheels and the ball, which solve the problem of slippery ball being stuck inside the launcher. The playing is not interrupted by the stuck ball and is enjoyable.

A transmitter includes a first limiter for performing a first clipping in which an amplitude part at a level higher than an amplitude level based on a first clipping level is cut out from an input signal to be subjected to power amplification, a second limiter for performing a second clipping in which an amplitude part at a level lower than the amplitude level based on a second clipping level is cut out from a first signal that has undergone the first clipping, a separation section for separating a second signal that has undergone the second clipping into an amplitude modulated part and a constant amplitude part, a first amplifier for amplifying the amplitude modulated part, a second amplifier for amplifying the constant amplitude part, and a combiner for outputting a third signal obtained by combining the amplitude part amplified by the first amplifier and the constant amplitude part amplified by the second amplifier.

A multiple adiabatic bed reforming apparatus and process involving stage-wise combustion and multiple reforming chambers. Co-flow and cross-flow occurs under laminar flow conditions. A reformer suitable for small scale production as well as large scale production. A passive stage-by-stage fuel distribution network suitable for low pressure fuel wherein the resistances in successive fuel distribution lines control the amount of fuel delivered to each combustion stage. Heat is captured from reforming syngas product to preheat gases before entering the reformer. Conditions that would produce unwanted coking or metal dusting are avoided or localized away from the heat exchangers to locations which can be cost effectively protected. A chemical reactor which has a core composed of a stack of metal plates that are diffusion bonded in face-to-face relationship.

A crystallisation facilitator for promoting crystal growth of a metal-organic framework, the crystallisation facilitator comprising at least one of: a metal or ionic form of that metal, or a compound including a metal, which is selected from the group consisting of Group 1 through 16 metals of the IUPAC Periodic Table of the Elements including actinides, and lanthanides, and combinations thereof. A method of synthesising a metal-organic framework using the crystallisation facilitator is also described.