The invention relates to a method and device for determining earphone noise reduction parameters and a computer readable medium. The earphone comprises a loudspeaker, an in-ear microphone and a filterassembly. The method comprises the following steps of: playing a low-frequency audio signal of which the frequency is out of the auditory range of human ears by a loudspeaker, determining a current transfer function of a transmission path from the loudspeaker to the in-ear microphone or a parameter of a current audio signal acquired by the in-ear microphone, determining a current noise reductionparameter based on the current transfer function or the parameter of the current audio signal by referring to N groups of corresponding relationships between a preset transfer function or a parameterof a preset audio signal and a preset noise reduction parameter, the noise reduction parameter comprising a filter coefficient of a filter enabled in the filter assembly, and configuring the filter assembly with the current noise reduction parameter to perform noise reduction. By playing the low-frequency audio signals beyond the auditory range of the human ears for many times, the dynamic adjustment of the noise reduction parameters of the earphone can be realized, the noise reduction effect in different use scenes is ensured, and meanwhile, the listening experience of a user is improved.