41 results about "Goby" patented technology

A fishing lure that has realistic motion when retrieved from a cast is presented. The lure has three sections: a head section, a tail section, and a mid-section. The head and tail sections are formed to resemble the head and tail areas of live bait. The mid-section has a series of segments that are joined by connectors that allow the segments to move back and forth when water impacts on the sidewalls of the segments. The overall shape of the fishing lure in one embodiment is a shad baitfish. Other embodiments include the overall shape of a lizard, a waterdog salamander, a blue gill fish, a goby fish, or a snake or worm.

The invention discloses a jellyfish breeding method including subjecting jellyfishes to mixed culture with ruditapes philippinarum, sinonovacula constrictas, penaeus chinensis, penaeus monodons and goby; controlling ecological breeding technologies including a stocking system based on breeding species and density, a feeding system based on a match between the species and number and a water quality control system based on a principle that firstly applying fertilizer into water, secondly maintaining fertilizer content and thirdly controlling the water quality. In this way, the jellyfishes and other species are rapid to grow and high in output and economic benefit. By the use of the jellyfish breeding method, the jellyfish output reaches 200-400kg / mu, the outputs of the ruditapes philippinarum and the sinonovacula constrictas reach 200-300kg / mu, and the outputs of the penaeus chinensis and the penaeus monodons reach 10-30kg / mu, and the goby output reaches 20-30kg / mu.

The invention discloses a marble goby starter feed and a preparation method thereof. The feed is prepared from, by mass, 20-50% of fish meal, 3-10% of shrimp shell meal, 3-10% of yeast, 10-30% of sandworms, 5-15% of sleeve-fish meal, 5-15% of spirulina, 15-30% of flour, 1-5% of soya-bean oil, 1-3% of phospholipid, 1-3% of glycine betaine, 1-2% of vitamin mixture, 2-4% of mineral element mixture, 1-5% of casein, 1-5% of compound exogenous enzyme preparation, 1-5% of microbial preparation and 1-2% of phagostimulant. The feed is designed and developed aiming at the nutritional demand law of marble goby fry, the particle size conforms to the high demand of marble goby fry culture, the feed attractiveness is high, the digestible absorptivity is high, the initial feeding rate and the immunity level can be remarkably strengthened, health growth of the fry is effectively promoted, and the survival rate of fish larvae is remarkably improved.

The invention discloses a mudskipper breeding bait nourishing agent, which is characterized by the following: using plant protein, animal protein, ammonium nitrate, potassium dihydrogenphosphate and silicate to make the product; providing organic nourishment and growing environment.

The invention provides floating expanded compound feed for marble goby, which comprises the following components: fish meal, bean pulp, flour, cuttlefish powder, shrimp bran powder, broad bean powder, beer yeast, choline chloride, betaine and fish oil. The feed is suitable for the marble goby above 50g for eating; pollution to an environment can be reduced; and flavor and the elasticity of meat quality of the marble goby can be improved.

The invention relates to a technology for breeding fish, in particular to a technology for breeding marble goby, which belongs to the technical field of aquiculture. The technology comprises maintenance of the pond, throwing and feeding of breeding baits, and the like. The field pond is used for cultivating hyacinth and narcissus, thereby reducing the content of ammonia nitrogen in pond water and keeping the water quality and the water temperature stable; and fish slurry mixed by iced fresh fish or eels is fed as the bait, thereby accelerating the growth of fries and providing the market with a byproduct which has the advantages of delicious flavor, high safety and reliability.

The invention discloses a marine fish water ecology toxicity test method. The method comprises the following steps of selecting gobies as tested fish species and domesticating in standard water which has a same water quality condition as a tested water sample; preparing a stock solution of the water sample to be tested; selecting a concentration gradient range through acute toxicity and chronic toxicity trial tests of the gobies, setting a concentration gradient with a small difference, taking sea water as a contrast group, and taking the water sample to be tested as a test group; simultaneously, carrying out acute toxicity and chronic toxicity tests of the gobies, recording changes in the gobies and the environment, carrying out variance analysis on data, and drawing a mortality curve of the gobies; and determining water quality toxicity through combining a physical condition of the gobies and water body index changes. Problems that current domestic research on a fish toxicity test is relatively simple, and there is aquatic ecotoxicity test method suitable for a salt-containing condition are solved.

The invention discloses a culture method of Thai Marble Goby, which comprises the following steps: (1), quicklime is used for disinfect a cultivating pool artificially before Thai Marble Goby is placed in the pool, and 250 kg to 500 kg of organic fertilizer is applied in each mu to cultivate water; and (2), fingerling of Thai Marble Goby is put in the pool, after that, freshwater shrimp and fingerling of Plagiognathops microlepis are put in to pool to be cultured with the fingerling of Thai Marble Goby. The culture method of Thai Marble Goby has the advantage that adult fish cultured through the method not only is large in size and high in specification, but also is high in survival rate and acre yield. According to the method provided by the invention, appropriate polyculture breeds are cultured and reasonable artificial feed are adopted to raise Thai Marble Goby, so that the specification and the weight of Thai Marble Goby are increased greatly, and then the acre yield of adult fish is improved.

The invention discloses a method for mixedly breeding sea cucumber and sepia esculenta in a pond. The method includes the steps of pond selecting, pond transforming, pond fertilizer and water preparation, young sea cucumber releasing, feed feeding, breeding management, sepia esculenta finishing and harvesting and subsequent sea cucumber breeding and harvesting. According to the method, crablat, shrimps, gobies and other enemies adverse to the sea cucumber can be caught and killed by the sepia esculenta, the utilization rate of water can be increased through mixed breeding of the sea cucumber and the sepia esculenta, and thus the sea cucumber and the sepia esculenta are bred in a mixed mode; by means of the method, the effects of value adding and synergia are achieved for the sea cucumber and the sepia esculenta, the utilization rate of water is increased, and the effects of purifying water and improving the breeding environment are achieved.

The invention belongs to the field of DNA markers in the field of aquaculture science and in particular relates to primers and a method for distinguishing Thai marble goby (oxyeleotrismarmoratus) fries from local marble goby (odontobutispotamophila) fries by adopting microsatellite markers. A pair of primer sequences is shown in SEQIDNo.8 and SEQIDNo.9. The method comprises the following steps of: carrying out PCR (polymerase chain reaction) amplification on the samples to be distinguished by utilizing primers; and then detecting the PCR products by using 1% of agarose gel electrophoresis andcarrying out EB (ethidium bromide) staining, wherein the samples with 250bp stripes are Thai marble goby and the samples with 300bp stripes are local marble goby. The method has the following beneficial effects: the microsatellite markers obtained by the method can carry out stable amplification in oxyeleotrismarmoratus and odontobutispotamophila and have stable results and clear stripes; and themethod is simple and convenient to operate and is low in cost and strong in practicability.

The invention relates to an extruded marble goby feed containing pupa protein ingredient, which is characterized in that in parts by weight, the composition of the feed includes: 6 to 8 parts of pupa protein liquid, 25 to 35 parts of flour, 20 to 25 parts of wheat middling, 8 to 15 parts of rapeseed meal, 2 to 5 parts of monocalcium phosphate, 2 to 5 parts of soybean oil, 10 to 15 parts of bone meal, 13 to 18 parts of corn, 10 to 16 parts of carrots, 5 to 12 parts of candy pomace, 7 to 15 parts of potatoes, 4 to 5 parts of egg shells, 0.08 to 0.12 parts of aqueous potassium permanganate solution with the concentration of 3 percent, 5 to 8 parts of honey water, 4 to 6 parts of Chinese herbal medicine extract and 2 to 5 parts of microelement powder. The extruded marble goby feed has the following advantages: besides containing richer ingredients, such as protein, vitamins and other mineral elements, than conventional feeds sold on the market, the extruded marble goby feed is also added with the Chinese herbal medicine extract, so that the immune function and disease resistance of marble gobies can be gradually enhanced by daily feeding, consequently, the survival rate can be increased, and economic benefit can be increased.

The invention discloses a marble goby trapping net, which comprises a fish inlet door enclosed by leaders, wherein three surfaces of the fish inlet door are enclosed by the leaders, and one surface of the fish inlet door is open; a sliding door is arranged on the fish inlet door; a sliding door rope is connected to the middle part of each of the two ends of the sliding door; the sliding door can slide to and fro to be opened and closed under the action of the sliding door ropes; a fish storage bag is connected to the leader on the front surface, and is fixedly connected through upper and lower fish storage bag net ropes, and an internal space of the fish storage bag is supported by three steel bar support rings until the fish storage bag extends to a zipper stake; three fish inlet internal barbs are arranged at the rear end of the sliding door and in an inner cavity of the fish storage bag respectively, and internally extend to be connected with three internal barb openings which are fixedly connected through internal barb opening ties respectively; three fixed opening plates are arranged at the top ends of openings of the three fish inlet internal barbs respectively, and are fixed by fixed opening rope stakes; the leaders, the sliding door, the fish storage bag and the internal barbs are fixedly connected through upper and lower net ropes, positioning stakes, the fixed opening rope stakes and the zipper stake.

The invention discloses a fish high-fat storage hepatocyte separation and culture method. The method comprises the following steps: a, selecting 45-55d young gobius giurinus, obtaining liver tissues of the young gobius giurinus under a sterile condition, and dispersing the liver tissues into blocks to obtain tissue blocks; b, putting the cell sieve with the pore diameter of 20 microns into a trypsin stop solution, and arranging the cell sieve with the pore diameter of 50 microns above the cell sieve with the pore diameter of 20 microns; c, putting the tissue blocks into a cell sieve with the pore diameter of 50 microns, continuously adding trypsin liquid into the cell sieve with the pore diameter of 50 microns to digest the tissue blocks, continuously digesting by flowing, collecting cellson the cell sieve with the pore diameter of 20 microns, and cleaning with 10% FBS to obtain high-fat storage liver cells; and d, inoculating the high-fat storage hepatocytes into a culture medium forculturing. The high-fat storage hepatocytes can be stabilized for one week after adherence, adipose cells can survive for about one month, and the high-fat storage hepatocytes can be used for cell level experiments in the period so that the problem that mature fish adipose cells are difficult to separate and culture is solved.

The present invention discloses a marble goby compound feed containing fermented silkworm chrysalis and a use thereof. The compound feed does not contain fish meal, and comprises fermented silkworm chrysalis, alpha-starch, wheat flour, wheat gluten flour, zeolite powder, calcium biphosphate, salt and premix for carnivorous fish. The fermented silkworm chrysalis is prepared by adding mixed meal into the silkworm chrysalis, inoculating microbe, adding carbon sources and inorganic salts, fermenting and culturing the mixture. The microbe is a mixed bacterio-liquid containing bacillus subtilis, bacillus acidi lactic and yeast. According to the present invention, the marble goby compound feed does not contain any fish meal and fish meat products, is produced from fermented silkworm chrysalis, can completely replace pure live fish and ice fish for breeding, increases survival rate and growth rate of marble goby, improves quality of fish meat, significantly increases the economic benefits of aquaculture, and promotes the sustained, healthy and stable development of marble goby aquaculture.

The invention discloses a method for acquiring ecological early breeding seeds of acanthogobius ommaturus Richardson, and relates to technologies for acquiring early seeds for early breeding for acanthogobius ommaturus Richardson. The method includes selecting parent fish; controlling the breeding quantities; selecting heat-insulation sheds of ponds; cultivating the parent fish through the winters; collecting fish fries. The method is characterized in that the parent fish with the weight ranging from 100 grams to 250 grams is selected in periods from November to December, and is cultivated in the ponds for takifugu rubripes through the winters, nylon films are constructed to form heat-insulation greenhouses on the upper sides of the ponds, the areas of the ponds preferably range from 3mu to 8mu, the ratio of female parent fish to male parent fish is 1:1, 10-12 pieces of parent fish is bred in each mu of the ponds under the control, 3-5 kilograms of live exopalaemon carinicauda are placed in each mu in winter cultivation periods and are used as fodders for the parent fish, water is changed regularly, an excellent water quality condition needs to be kept, 1.2-meter water levels of the ponds are kept, and the salinity of the water in the ponds keeps ranging from 6%o to 15%o; the fish fries are sampled after 20th of March in the next year, growth conditions of the fish fries are inspected, and the fish fries are timely collected and distributed when the sizes of the growing fish fries range from 4 centimeters to 6 centimeters.

A walking goby digging machine comprises an armrest, a machine body, a water pipe, a motor, a water pump, a transmission shaft, a net cage, a rotating shaft, digging knives, a support bar, and a water injector, wherein, the armrest is installed on the machine body; the wheels are made into roller's shape; the motor and the water pump are installed in the machine body; the net cage is installed in the front of the machine body; two symmetric digging knives are installed on an axial plane of the rotating shaft; the digging knives are iron wire weaved into nets and are installed on the rotating shaft in a slight backward inclination way; the motor in the machine body is connected to the rotating shaft through the transmission shaft; the outlet pipe is provided with the water injector extending the net cage and the digging knives; and the water injector is made in a flat trumpet shape. When one is digging a fish, the walking goby in the slush with the mud is dug out by the digging knives and is thrown to the net cage backwards; the water in the water injector is injected to the surface of the digging knives to wash the surface clean and flush the slush in the net cage away at the same time, and only the fish the left; the machine body is made into self-propelling type; the water pump is detachable from the machine body; the digging knives can be used to dig shellfish by making the digging knives into nail rakes. The walking goby digging machine has a simple structure.

The invention belongs to the fish mitochondrial genome research field, and concretely relates to a COIII and ND3 gene amplimer, the gene amplimer is composed of two strips of single chain oligonucleotide chains, wherein a light chain primer is a nucleotide sequence shown in SEQ ID NO.1, and a heavy chain primer is a nucleotide sequence shown in SEQ ID NO.2. The invention also provides a design and an amplification method of the amplimer. The invention can specifically amplify a plurality of goby mitochondrion COIII and ND3 gene sequences with high efficiency, and can be used in goby different classification category systems evolution and classification research.

The present invention provides a globefish fodder capable of raising the tetrodotoxin content inside globefish. The fodder may be fresh globefish internal organ 5-60 wt% and fresh fingerling and shrimp 40-95 wt%; fresh globefish internal organ after extracting tetrodotoxin 5-80 wt% and fresh fingerling and shrimp 20-95 wt%; goby, turbellarian worm, swordfish, etc. 5-50 wt% and fresh fingerling and shrimp 50-95 wt%; and fresh toad, scorpion, spider and snake 3-30 wt% and fresh fingerling and shrimp 70-97 wt%. The fodders containing biotoxin may be used alone or after being mixed together.

The invention belongs to the fish mitochondrial genome research field, and concretely relates to a ND1 gene complete sequence amplimer, the gene amplimer is composed of two strips of single chain oligonucleotide chains, wherein a light chain primer is a nucleotide sequence shown in SEQ ID NO.1, and a heavy chain primer is a nucleotide sequence shown in SEQ ID NO.2. The invention also provides a design and an amplification method of the amplimer. The invention can specifically amplify a plurality of goby mitochondrion ND1 gene total sequences with high efficiency, and can be used in goby different classification category systems evolution and classification research.

The invention belongs to the field of DNA markers in the field of aquaculture science and in particular relates to primers and a method for distinguishing Thai marble goby (oxyeleotrismarmoratus) fries from local marble goby (odontobutispotamophila) fries by adopting microsatellite markers. A pair of primer sequences is shown in SEQIDNo.8 and SEQIDNo.9. The method comprises the following steps of: carrying out PCR (polymerase chain reaction) amplification on the samples to be distinguished by utilizing primers; and then detecting the PCR products by using 1% of agarose gel electrophoresis and carrying out EB (ethidium bromide) staining, wherein the samples with 250bp stripes are Thai marble goby and the samples with 300bp stripes are local marble goby. The method has the following beneficial effects: the microsatellite markers obtained by the method can carry out stable amplification in oxyeleotrismarmoratus and odontobutispotamophila and have stable results and clear stripes; and the method is simple and convenient to operate and is low in cost and strong in practicability.

The present invention discloses an aquatic feed granulating device for marble goby. The aquatic feed granulating device comprises a granulating machine main body; a working chamber is arranged insidethe granulating machine main body; a blower is arranged on the inner bottom end, close to one side of the working chamber, of the granulating machine main body; a second motor is arranged on the innerbottom end, close to one side of the blower, of the granulating machine main body; an inspection door is arranged on the front surface of the granulating machine main body; a material inlet is arranged at the upper end of the granulating machine main body; a second material outlet is arranged at the rear surface of the granulating machine main body; and a solenoid valve is embedded in the rear surface, close to the upper end of the second material outlet, of the granulating machine main body. An electric hydraulic rod and a push plate are arranged, so that the granulating device does not require too much manual operation and saves time and labor. A humidity sensor and the blower are arranged to effectively solve a moisture problem of feeds. The second motor and stirring blades are arranged to avoid a phenomenon of excessive accumulation of the aquatic feed inside the working chamber.

The invention belongs to the field of fish mitochondrial genome research, and specifically relates to a COⅢ and ND3 gene amplification primer, which is composed of two single-stranded oligonucleotide chains, wherein the light chain primer is the nucleotide shown in SEQ ID No.1 sequence; the heavy chain primer is the nucleotide sequence shown in SEQ ID No.2. The invention also provides the design and amplification method of the amplification primer. The invention can efficiently and specifically amplify the mitochondrial COⅢ and ND3 gene sequences of various gobies, and can be applied to the evolution and classification research of different taxonomic stages of the gobies.

An indoor artificial breeding method of glossogobius giuris comprises the steps of indoor intensive breeding of parent glossogobius giuris, artificial ripening and hastening, artificial hatching and offspring seedling breeding. The indoor artificial breeding method is characterized in that after intensive overwintering breeding of the glossogobius giuris, the artificial hastening is performed, a lutropin releasing hormone A2 and a human chorionic gonadotropin are used as hastening hormones, after ripening and hastening of male and female parent glossogobius giuris, zygotes adhere to lining meshes of laying nests, the meshes to which the glossogobius giuris zygotes adhere are taken out, unfolded evenly and vertically hung in a hatching pond, after release, rich water seedling culture is adopted for primarily hatching yellow seedlings, after seawater rotifers are replaced by artemia nauplii, the seedlings are transferred to a water nursery pond of 10-15 m<2> for breeding, the seedlings are fed with artemia nauplii, blood worms, cyclops or opossum shrimps, and when juvenile glossogobius giuris grows to 15-25 mm in total length, the conventional fingerling breeding can be performed.

The invention relates to a method for ecologically preventing and treating epizootic ulcerative syndromes of fishes, in particular to a method for ecologically preventing and treating epizootic ulcerative syndromes of fishes, particularly hybrid northern snakeheads or marble gobies (marbled sand gobies). The method includes culturing sick fishes in water with biological floccules. The method has the advantages that as proved by repeated experiments, the epizootic ulcerative syndromes of the fishes can be effectively prevented and treated by the method, and an effective rate reaches 70%; the cost is low, environments can be protected, drugs, chemical reagents and other extra reagents can be omitted, the effective rate is high, the method is simple, and operation is easy; intake of other drugs with side effects can be prevented when the method is used for preventing and treating the epizootic ulcerative syndromes for the fishes, and accordingly the food safety can be effectively guaranteed for people.

The invention discloses a method for acquiring ecological early breeding seeds of acanthogobius ommaturus Richardson, and relates to technologies for acquiring early seeds for early breeding for acanthogobius ommaturus Richardson. The method includes selecting parent fish; controlling the breeding quantities; selecting heat-insulation sheds of ponds; cultivating the parent fish through the winters; collecting fish fries. The method is characterized in that the parent fish with the weight ranging from 100 grams to 250 grams is selected in periods from November to December, and is cultivated in the ponds for takifugu rubripes through the winters, nylon films are constructed to form heat-insulation greenhouses on the upper sides of the ponds, the areas of the ponds preferably range from 3mu to 8mu, the ratio of female parent fish to male parent fish is 1:1, 10-12 pieces of parent fish is bred in each mu of the ponds under the control, 3-5 kilograms of live exopalaemon carinicauda are placed in each mu in winter cultivation periods and are used as fodders for the parent fish, water is changed regularly, an excellent water quality condition needs to be kept, 1.2-meter water levels of the ponds are kept, and the salinity of the water in the ponds keeps ranging from 6%o to 15%o; the fish fries are sampled after 20th of March in the next year, growth conditions of the fish fries are inspected, and the fish fries are timely collected and distributed when the sizes of the growing fish fries range from 4 centimeters to 6 centimeters.

The invention discloses a jellyfish breeding method including subjecting jellyfishes to mixed culture with ruditapes philippinarum, sinonovacula constrictas, penaeus chinensis, penaeus monodons and goby; controlling ecological breeding technologies including a stocking system based on breeding species and density, a feeding system based on a match between the species and number and a water quality control system based on a principle that firstly applying fertilizer into water, secondly maintaining fertilizer content and thirdly controlling the water quality. In this way, the jellyfishes and other species are rapid to grow and high in output and economic benefit. By the use of the jellyfish breeding method, the jellyfish output reaches 200-400kg / mu, the outputs of the ruditapes philippinarum and the sinonovacula constrictas reach 200-300kg / mu, and the outputs of the penaeus chinensis and the penaeus monodons reach 10-30kg / mu, and the goby output reaches 20-30kg / mu.