The invention discloses microdiet for fish larvae of takifugu rubripes. The microdiet comprises components as follows: white fish meal, tenebrio molitor meal, earthworm powder, shrimp oil, rice protein, husked sorghum flour, lotus root starch, maize germ oil, walnut oil, casein hydrolysate, green-lipped mussel extract, cylindrotheca powder, haematococcus pluvialis powder, a functional additive, complex vitamins and compound minerals. The preparation method of the microdiet comprises steps as follows: 1), preparation of raw materials; 2), synthesis of biological materials, 3), conversion into cooked materials; 4), puffing for granulation; 5), drying; 6), spraying for film coating; 7), vacuum drying. The microdiet can improve the body color of the fish larvae of the takifugu rubripes, promote growth and reduce tetrodotoxin enrichment.