Primer and method for quickly detecting gender difference of juvenile fishes of fugu rubripes
A technology for the sex of redfin puffer and juvenile fish, applied in biochemical equipment and methods, microbe determination/testing, DNA/RNA fragments, etc.
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[0031] The method for rapidly detecting the sex difference of puffer puffer juveniles described in the present invention comprises the following steps:
[0032] 1. Sample collection
[0033] In this example, 20 puffer juveniles of different sexes were used as the research objects, and all 20 individuals were randomly selected from a group of puffer juveniles cultured by an aquatic company. The gonad tissues of each individual were fixed in Bouin’s solution for 24 hours, rinsed with 50% ethanol and stored in 70% ethanol; the fin ray tissues were cut and stored at -20°C.
[0034] 2. Gonad tissue section and sex differentiation identification of redfin puffer juvenile
[0035] The fixed gonad tissue samples of juvenile redfin puffer were sliced by conventional methods, with a thickness of 4-7 μm, and stained with H.E. Observe and take pictures with OLYMPUS DP72 microscope to measure the size of germ cells.
[0036] The oocyte is oval in shape, significantly larger than the o...
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