151 results about "Female individual" patented technology

A method is provided for enhancing a female individual's sexual desire and responsiveness. The method involves administration of a pharmaceutical formulation containing an effective amount of an androgenic agent, wherein administration is on an as-needed basis rather than involving chronic pharmacotherapy. Local delivery may be accomplished via administration to the vagina, vulvar area or urethra of the individual, although oral administration is preferred for those androgenic agents that are orally active. Formulations and kits for carrying out the method are provided as well.

This invention relates to devices targeted to resolving various conditions of a female patient in need thereof, the conditions being female sexual dysfunction and urinary incontinence. The present disclosure provides for a device which incorporates a variety of elements in an effort to treat certain conditions of a female individual in need thereof. Optionally, the device of the present invention may be used as a prophylactic measure to prevent a condition selected from the group consisting of female sexual dysfunction and urinary incontinence.

A method is provided for treating a female individual who suffers from or is prone to dyspareunia. The method involves vaginal and / or vulvar administration of a formulation comprising a therapeutically effective amount of a carrageenan and a pharmaceutically acceptable aqueous carrier. In addition, a carrageenan-based formulation is provided as a new composition of matter. Packaged kits for an individual to use in the administration of a carrageenan-based formulation as provided as well.

The invention discloses a cultivation method of a novel strain of crassostrea gigas with a pure purple left shells. The cultivation method is characterized in that operation comprises the steps of: (1) seed production: selecting male and female individuals with pure purple left shells from a cultured group as cultivating parent shellfishes; performing one-to-one insemination and copulation, respectively marking breeding systems, cultivating larva by adopting strict isolation measures, and placing in a same sea area for intermediate culture and cultivation; breeding generation by generation to purify so as to form a family; and (2) purification of the novel strain: selecting inseparate families with character proportion of above 90% as parent shellfishes, performing seedling propagation so as to cultivate the novel strain of crassostrea gigas with the pure purple left shells and stable character. With the adoption of the cultivation method provided by the invention, the cultivating process of the novel strain is accelerated and transgenic and pharmaceutical treatment do not need to be carried out, the novel strain is cultivated, the additional value of product is also improved through color character, and the market sale price and income of cultivation enterprises and cultivators are increased, and market prospect is wide.

The invention belongs to the technical field of the breeding of poultry. A cultivation method of yellow-feathered meat chickens with a higher production performance sequentially comprises the following: 1, taking the yellow-feathered meat chickens characterized in yellow hemp feathers and yellow shins as male parents, taking abroad broiler chickens as female parents, producing an F1 generation through hybridization, and retaining female chickens of the F1 generation; 2, taking the yellow-feathered meat chickens which serve as the male parents in the step 1 as male parents, mating the male parents with the female chickens of the F1 generation, producing an F2 generation through hybridization, and selecting and retaining individuals with the yellow hemp feathers in the F2 generation, and feeding and management; 3, crossing and reproducing the male and female individuals obtained in the step 2, and purifying and fixing beneficial properties according to a family tree and an individual selection method; and 4, forming a new breed of yellow-feathered meat chickens containing the blood relationship of the abroad broiler chickens through the selection and breeding of the beneficial properties of 4 or more generations. In the invention, the growing speed of the yellow-feathered meat chickens in China in the early period is quite obviously improved, the feed / gain ratio is reduced, and the reproductive performance is improved to a certain extent.

The invention discloses technology for culturing a unisexual hybrid scallop by utilizing a purple scallop and a bay scallop, which relates to shellfish breeding technology for aquiculture. The technology comprises the steps of: utilizing two congeneric hermaphroditic scallops with same chromosome number; ripening the two scallops respectively to achieve the synchronous maturity; and then synchronously inducing the two scallops to spawn and spermiate; and acquiring sperms and ovum of the two scallops respectively for interspecies cross-insemination to culture the unisexual hybrid scallop. Obtained offsprings have separated sexes, have males and females, and also have hermaphroditic individuals; ovum from female individuals in the hybrid offsprings can be inseminated by any parental sperm and normally grow; and the hermaphroditic individuals in the hybrid offsprings can be used for quickly purifying a strain.

The invention is a method for rapid identifying genetic sex of tongue sole, comprising rapid extracting DNA of tongue sole, designing and synthesizing of LAMP specific primer, establishing a LAMP reaction system, detecting of LAMP reaction products and identifying genetic sex. By female specific molecular marker sequence of tongue sole, the invention designs specific primer, performs transitory nucleic acid amplification under constant temperature, the genetic female individual produces specific DNA fragment, while the male individual does not produce such DNA amplification product. The invention creates a LAMP method for rapid detecting genetic sex of tongue sole for the first time, can be used in culture farm, and the testing operation can be finished within 2.5h. The invention has the characteristic of being advanced, rapid, specific, precise, sensitive, simple and in no need of large-scale instruments, thus providing a new technological approach for fish sex detection and control. The invention has important application value in control of tongue sole sex and production of unisex fry, and wide application prospect in identification of genetic sex of other fish species and research of sex control.

The invention discloses a seed production method for Portugal oyster quick-growing seed selection. Full weight of the Portugal oysters work as a seed selection target, the first 5% to 6% of full weight of male and female individuals are selected from a plurality of Portugal oyster individual with different geographical groups for breeding parent clams; through continuous generations of oriented seed selection, high-quality oysters with fast growing speed and strong stress resistance as well as high survival rate can be screened. In the seed production method, the Portugal oyster full weight works as the seed selection target and group seed selection and family seed selection technologies are employed to breed Portugal oyster types with fast growing speed, strong stress resistance and high survival rate; an important role is played for Portugal oyster cultivation output and economic benefit improvements; and a foundation is laid for Portugal oyster new type development and genetic improvement.

The invention discloses a breeding method for amphiprotic fertile autotetraploid crucian carps and an establishment method for strains of the crucian carps. The breeding method includes the following steps that allotetraploid crucian carps are selected from crucian carp hybridization F1 offspring with red crucian carps as female parents and megalobrama amblycephala as male parents, female individuals capable of generating ova of different sizes and male individuals capable of generating watery semen are selected from the allotetraploid crucian carps for hasten parturition, insemination is carried out manually through a dry method, running water incubation is conducted on fertilized ova, then the fertilized ova are bred, and the amphiprotic fertile autotetraploid crucian carps are selected from selfing offspring. Selfing is carried out on the male individuals and the female individuals in the obtained autotetraploid crucian carps, and the strains of the amphiprotic fertile autotetraploid crucian carps can be established and obtained through multi-offspring breeding. Ploid hybridization is conducted on the autotetraploid crucian carp colonies obtained through the breeding method and diploid crucian carps, so allotriploid fishes can be bred on a large scale. The strains of the crucian carps obtained through the breeding method are excellent in character, stable in heredity and high in fertilization rate and hatchability, and the breeding method has a great significance in genetic research of fishes.

The invention belongs to the technical field of poultry breeding. A method for breeding a new strain of black-bone chicken with higher production performance comprises the following steps of: step 1, taking parents of foreign broiler chicken as female parents instead of hen CD, and the black-bone chicken with jute feather inland as male parents, and generating a generation F1 through cross breeding, selecting and leaving individuals with jute feather, black bone, and black skin in the generation F1; step 2, crossing and propagating the female individuals and the male individuals obtained in the step 1, and separating out jute feather, recessive white feather, black feather, and reed catkins, and removing recessive white feather, from the colored feather colony through crossing, and purifying and fixing the hair color, black bone, black shin, sexual maturity and other appearance characters according to the family and individual selective method, improving the growing speed, reproductive performance and other economic characters; step 3, through the breeding of four or more generations, successfully breeding the new strain of the black-bone chicken with relatively higher production performance in jute feather, recessive white feather, black feather, and reed catkins. The method improves the early growing speed and egg laying performance of the black-bone chicken in the nation, and obviously reduces the feed / gain ratio.

A non-invasive method of altering vaginal blood flow in a female individual to augment or lessen sexual arousal is provided. The method involves administering an individual odorant or odorant mixture for inhalation that is sufficient to alter vaginal blood flow of the female by about −20% to about +30% compared to a baseline vaginal blood flow without inhalation of the odorant. Also provided is a method for screening an odorant for its capacity to increase or decrease vaginal blood flow, and an article of manufacture, or kit, containing an odorant and instructions for its use in altering vaginal blood flow.

The invention discloses a primer and a method for quickly detecting gender difference of juvenile fishes of fugu rubripes. A pair of outer PCR (Polymerase Chain Reaction) primers which respectively amplify gender difference gene sequences and an inner PCR primer which contains mispaired basic groups aiming at mutation sites are designed aiming at G-C mononucleotide mutation sites existing on gender differential genes of male and female individuals of fugu rubripes. By means of the primer, different amplification results can be obtained by different gender DNA (Deoxyribonucleic Acid) samples of fugu rubripes, so that the gender difference of fugu rubripes can be judged. The primer disclosed by the invention is low in cost, simple to operate, quick and accurate and suitable for popularization and application, can quickly identify gender of juvenile fishes of fugu rubripes on a basis of extracting genome DNA of fugu rubripes one year old, and has important application value to accelerate the unisexuality breeding process of fugu rubripes and PCR detection method research in the field of molecular biology.

The invention provides a method and a system for acquiring an age of a female individual of Chinese population. The method comprises the following steps: extracting DNA (Deoxyribonucleic Acid) of the female individual, acquiring methylation indexes of 11 CpG loci of the DNA, conducting regress analysis to the methylation indexes of each GpG loci, the 11 CpG loci and the age with SPSS 17 (Statistic Package for Social Science) software, and constructing a regression model to obtain the age of the female individual of the Chinese population. The scheme provided by the invention can accurately obtain the age of the female individual of the Chinese population, deduces the age of the individual with a biologically examined blood or blood stain sample remained in a crime scene of the public security actual combat, so as to facilitate targeting of the range of criminal suspects, provide clues for investigation of the case, and increase the detection speed of the case.

A non-invasive method of altering vaginal blood flow in a female individual to augment or lessen sexual arousal is provided. The method involves administering an individual odorant or odorant mixture for inhalation that is sufficient to alter vaginal blood flow of the female by about −20% to about +30% compared to a baseline vaginal blood flow without inhalation of the odorant. Also provided is a method for screening an odorant for its capacity to increase or decrease vaginal blood flow, and an article of manufacture, or kit, containing an odorant and instructions for its use in altering vaginal blood flow.

The invention belongs to the technical field of poultry breeding, and relates to a method for cultivating a special male parent strain mainly used as yellow-feather broilers, which comprises the following steps of: 1, hybridizing to generate a F1 generation by taking a broiler chicken from another country as a male parent and a yellow-feather broiler with characteristics of jute feathers and yellow shins as a female parent, selecting and remaining F1-generation cocks and performing feeding and management according to a feeding and management method of breeding hens; 2, mating a F1-generation cock serving as the male parent with the female parent in the step 1 to generate a F2 generation, grouping respectively according to feather colors in the F2 generation, performing test cross, and performing the feeding and management according to the feeding and management method of the breeding hens; 3, crossing male and female individuals of each group obtained in the step 2 athwart and propagating, and purifying and fixing beneficial characters according to a family and individual selection method; and 4, breeding by four or more than four beneficial characters for generations to form a new broiler strain containing the bloodlines of the broiler chickens from other countries. By the method, the growth speed of the yellow-feather broilers in China is improved substantially, and the feed conversion rate is reduced.

The invention discloses a breeding method for a high-yield new variety line of recessive white-feather gooses and application thereof. The method comprises the following steps of: selecting the high-yield white-feather gooses without broodiness or with weak broodiness, and eliminating dominant white-feather individuals and keeping recessive white-feather individuals A by the test cross or the molecular marker method; self-reproducing the male individuals and female individuals of A to breed the recessive white-feather gooses B; and breeding B again, eliminating dominant white-feather homozygote and heterozygote, self-reproducing the recessive white-feather high-yield individuals with homozygous genotype to expand the group, building a family and breeding the family until the high-yield recessive white-feather goose new variety line C is bred, and performing dualistic or polyphyletic mating by taking the C as a female parent and a colour-feather local goose as a male parent to produce colour-feather gooses or simulated local gooses.

The invention provides a method for cultivating green-shell-egg black-feather black-bone chicken. The method includes the following steps that 1, purebred Dongxiang black-feather green-shell egg chicken serve as female parents, purebred Taihe silky black-bone chicken serve as male parents, the female parents and the male parents are hybridized to generate F1 generations, full-black male and female individuals with the full-black feathers and the black-color characteristics in the F1 generations are selected and remained; 2, the male and female individuals selected and remained in the step 1 are transversely mated and propagated, and individual recording and family breeding are permitted; 3, according to the compatriot selection method, the family selection method and the individual selection method, black-feather, black-color-characteristic and full-black hens generating green shell eggs at the same time are selected and remained in F2 generations generated in the step 2, and black-feather, black-color-characteristic and full-black cocks capable of allowing the hens to generate green shell eggs are selected and remained in the F2 generations; 4, the male and female individuals selected and remained in the step 3 are transversely mated and propagated and bred for 5 generations to 6 generations, and black-feather black-bone chicken generating green shell eggs are cultivated.

The invention discloses a primer for sex identification of Nile tilapia. The primer can specifically amplify AMH genes of Nile tilapia, a male individual generates two specifically amplified segments, lengths of the specifically amplified segments are 179bp and 412bp respectively, and a female individual generates a specifically amplified segment of 412bp in length. The primer is used to amplify genome DNA of Nile tilapia, the male individual generates the two specifically amplified segments of 179bp and 412bp in length, the female individual generates the specifically amplified segment of 412bp in length, and the male individual and the female individual can be distinguished obviously, so that sexes of Nile tilapia can be identified simply and accurately. The primer is high in specificity and does not amplify other segments except for a target segment. The invention further discloses a PCR (polymerase chain reaction) identification method using the primer. The PCR identification method is quick to operate and accurate in result, and the defect of unstable results caused by adopting conventional morphological methods to identify Nile tilapia different in sex is avoided.

The invention discloses primers and a method for quickly detecting Takifugu obscurus young fish sex difference single base mutation. According to the mononucleotide mutant sites on sex difference genes of Takifugu obscurus male and female individuals, a pair of external PCR (polymerase chain reaction) primer and internal PCR primer containing mispaired base for the mutant sites are designed to respectively amplify the sex difference gene sequences. By utilizing the primers, different sexes of DNA (deoxyribonucleic acid) samples of Takifugu obscurus can obtain different amplification results, thereby judging the sex difference of Takifugu obscurus. The method has the advantages of high speed and high accuracy, is simple to operate and suitable for popularization and application, can quickly and accurately identify the sex of the Takifugu obscurus young fish on the basis of extracting genome DNA (deoxyribonucleic acid) of Takifugu obscurus under 1 year, and has important application values in accelerating the Takifugu obscurus unisexual breeding progress and researching the quick PCR detection method in the field of molecular biology.

A method for breeding a double-limitation multi-silk-quantity domestic silkworm breed includes the following steps: using a multi-silk-quantity breed for spring 'Jingsong' as a recurrent parent, conducting hybridization to a stripe sex-limited character donor parent through a sex-limited breed '755' female individual which is saved as a source, and then obtaining a sex-limited breed JsQXian; using a multi-silk-quantity breed for spring 'Haoyue' as a recurrent parent, conducting hybridization to the stripe sex-limited character donor parent through the sex-limited breed '755' female individual which is saved as the source to conduct economical character selection to each generation in the breeding process, and then obtaining a sex-limited breed HyQXian; enabling the target material JsQXian and the target material HyQXian to conduct hybridization, and then directly obtaining the double-limitation multi-silk-quantity domestic silkworm breed. The method is adopted to select a genetic background of the recurrent parent and a target character of the donor parent in a marker-assisted selection mode, and the new double-limitation multi-silk-quantity domestic silkworm breed which is high in yield, good in quality, and labor-saving in the reproductive process can be bred within 3-4 years quickly and high-efficiency.

The invention belongs to the field of fish sex determination in the field of aquaculture, and particularly relates to a specific DNA fragment SSM2 for sex determination of sturgeons and application. According to the invention, a specific DNA fragment of sturgeon female individuals is obtained through a high-throughput sequencing and genome comparison method. Primers are designed for the fragment,and can perform accurate and rapid sex identification on nine kinds of sturgeons. An identification method of the invention has the characteristics of simple, quick and accurate operation, extremely small damage to the sturgeons and the like, solves the problem of sturgeon sex identification, and is helpful for rapid development of the sturgeon culture industry.

The invention provides a male specific DNA marker of oplegnathus punctatus, wherein the sequence of the DNA fragment on a Y chromosome is shown in SEQ ID NO: 1; the sequence of the DNA fragment on a Xchromosome is shown in SEQ ID NO: 2. The DNA fragment on the Y chromosome is 12 bp more than the fragment on the X chromosome, and the fragment is the DNA fragment peculiar to the Y chromosome. According to the invention, two homologous and different DNA fragments on the X and Y chromosomes are screened from the whole genome sequence of the oplegnathus punctatus, and a method for identifying theheredity sex of the oplegnathus punctatus is further established; and the method can be used for quickly, accurately and effectively distinguishing the heredity sex of the oplegnathus punctatus. According to the method, a specific target band amplified in a male individual cannot be amplified by a female individual, and the product can be resolved by agarose electrophoresis, so that the time for accurately identifying the heredity sex of the oplegnathus punctatus is shortened, and the method is suitable for identifying the heredity sex of the oplegnathus punctatus in a simple environment of aculture farm, and the detection time and the cost are saved. The method has important significance and application value in sex identification, seedling breeding and family selection and breeding of the oplegnathus punctatus.

The invention discloses a rotational mating breeding method for pinctada fucata groups. The method comprises the following steps of: collecting at least three pinctada fucata groups from different sources, and constructing an F1 generation diallel crossing group; before a breeding season, randomly sampling over 1,000 individuals from every each group for performing character measurement, computing a breeding character average value or a multi-character comprehensive selection index, sequencing according to sizes, and at least selecting five groups serving as cone breeding groups by adopting a last-one-out system; selecting largest female and male individuals from every each selected group, mating male individuals of a No.1 group with female individuals of a No.2 group, mating male individuals of a No.2 group with female individuals of a No.3 group, and so on, and rotationally mating, wherein the ratio of female individuals to male individuals is 2:1, and at least four male individuals are selected; and breeding generation after generation to over five generations. Due to the adoption of the method, high selection strength is realized, genetic gain is increased, and the genetic improvement of every each generation of the bred pinctada fucata can be over 15 percent.

The invention belongs to the technical field of poultry breeding. The invention provides a method for breeding a high-quality broiler strain with higher production performance, recessive white feathers and colored feathers. The method comprises the following steps: 1. taking the foreign broiler chicken as female parents and yellow-feathered broilers with the characteristics of jute feathers and yellow shins as male parents to hybridize to generate F1 generation, selecting and reserving the individuals with jute feathers in the F1 generation, and feeding and managing the individuals in the ways of feeding and managing the breeding chicken; 2. carrying out athwart crossing and propagating on the obtained male and female individuals with jute feathers in the step 1, grouping the generated individuals respectively according to the feather colors, carrying out test crossing on the groups of individuals with other feather colors except the group of individuals with white feathers, and then purifying and fixing the beneficial characters of the groups according to the family and individual selection methods; and 3. forming the new strain with the foreign broiler chicken bloodline through systematic breeding of four or more generations of chicken. The method has the beneficial effects of substantially improving the early growth speed of the yellow-feathered broilers in China and more obviously reducing the feed-gain ratio.

The invention belongs to the field of biotechnology (genome editing), and relates to a method for inserting exogenous genes into W chromosomes of bombyx mori in a fixed-point mode through mediating of transcriptional activator-like nuclease, and application of the method to bombyx mori sex discrimination. The method for inserting exogenous genes into W chromosomes of bombyx mori in the fixed-point mode specifically comprises the steps that W chromosome molecular markers in a bombyx mori genome database are analyzed, a TALENs targeting site is screened, TALENs mRNA expression plasmid is designed and obtained by synthesis, homologous recombinant carrier plasmid for expressing exogenous (reporter) genes (such as red fluorescent protein) is constructed, the microinjection technique is used for injecting the TALENs mRNA expression plasmid and the homologous recombinant (donor) carrier plasmid into eggs of the bombyx mori Nistara strain, the exogenous genes are inserted into the W chromosomes of bombyx mori in the fixed-point mode, the exogenous genes are only expressed in female individuals, and thus the purpose of rapid and efficient sex discrimination is achieved.

The invention discloses SNP primers for early screening a strongylocentrotus intermedius fine variety and a screening method. The screening method comprises the following steps: selecting a strongylocentrotus intermedius second-generation male individual (Mars symbol) self-bred in Japan and a female individual (Venus symbol) bred for more than five generations in Longwangtang, Lvshun, China as male and female parents to crossbreed an F1 generation; establishing a mapping population containing 194 F1-generation individuals and two parents; cultivating in the same environment, extracting DNA, sequencing and taking other technical measures to find genetic markers of strongylocentrotus intermedius for the first time and design the relevant primers, wherein the primers can be used for early screening the strongylocentrotus intermedius fine variety. Compared with the prior art, the screening method has the advantages as follows: by the screening method, not only can manpower and material resources be saved and the seed selection be reduced, but also good genetic genes of the parents can be ensured, so that good progeny traits can be ensured.

The invention relates to SNP sites for sex determination of Scylla paramamosain and an identification method thereof. A base sequence is SEQ ID No. 1 or SEQ ID No. 2 and has a length of 285 bp; and the SNP sites are site 62, site 70, site 74, site 145, site 147, site 163, site 186 and site 194 in the SEQ ID No. 1 or SEQ ID No. 2. The identification method comprises the following steps: extraction of the genome DNAs of male and female individuals; construction of a reduced genome library and high-flux sequencing; sequence analysis and screening of candidate SNP markers linked with sexes; and large-sample verification of the candidate SNP markers. According to the invention, the sex-linked molecular markers of Scylla paramamosain are identified for the first time and have great guidance significance to the sex determination and differentiation mechanism of Scylla paramamosain and to research on unisexual breeding. The method provided by the invention has the advantages of accuracy, sensitivity, reliability and the like, has good universality, does not need to learn about genome information in advance and is applicable to a great number of species without reference genomes, so the method has good promotion and application potential.