42 results about "Y chromosome" patented technology

The invention discloses a method for noninvasive antenatal diagnosis through separating fetal nucleated red blood cells from peripheral circulating blood of a pregnant woman. The method comprises the following steps: 1, preprocessing fresh pregnant woman maternal peripheral blood: separating and enriching monocytes containing fetal nucleated red blood cells from maternal peripheral whole blood through a density gradient centrifugation technology; 2, capturing cells: adding a separated monocyte suspension to a substrate chip modified with an antibody, and carrying out standing capturing; and 3, identifying the sex of a fetus: identifying whether the nucleated red blood cells of the captured fetus contain Y chromosome or not by using an FISH probe technology (CSP-X / Y). The method for noninvasive antenatal diagnosis through separating fetal nucleated red blood cells from peripheral circulating blood of the pregnant woman can realize capture and identification of the fetal nucleated red blood cells from the maternal peripheral circulating blood, and allows the FISH analysis to be directly carried out in order to realize noninvasive antenatal diagnosis of the sex or 21-Trisomy syndrome and other chromosome diseases of the fetus.

The invention provides a kit for detecting the abnormal number of chromosomes which causes natural abortion. The kit comprises 7 to 9 groups of multiple PCR primers which can carry out molecule detection and source judging on the abnormal number of 10 types of chromosomes (the chromosomes of number 2, number 13, number 15, number 16, number 18, number 20, number 21, number 22, X and Y); and the detection range covers the common abnormal chromosome types during natural abortion and prenatal diagnosis and the kit has the advantages of high delicacy, high accuracy, low detection cost, and the like.

The invention relates to a method for efficiently producing YY transformed females of Nile tilapia with a high reproductive rate. Firstly, the XY transformed females are mated with XY males or YY super males, and the offspring are simultaneously fertilized 5 days after fertilization. Soak in estrogen and androgen synthase inhibitors until 10 days after insemination; then feed high-dose hormone-supplemented feed for feminization for 3 months after juveniles open, and use Y chromosome-specific molecular markers to screen out sex chromosomes The transformed female fish with genotype YY were fed with normal feed until 6 months old; then at the age of 6-7 months, the YY transformed female fish were treated with low-dose hormone-supplemented feed, and were raised normally until sexual maturity; finally, the fertile YY transformed female fish were screened out . Fertile YY transformed females are mated with YY super males to establish YY maintenance lines and realize large-scale production of genetic all-male fry, which can significantly increase aquaculture production, reduce aquaculture costs, and reduce the use of hormones. In areas where tilapias invade and flood A large number of YY super male fish can be released for biological control.

The invention relates to an amplification composition for detecting abnormal number of chromosomal aneuploid and a rapid detection kit, and belongs to the technical field of biology. The amplification composition can be used for amplification detection of STR sites related to 8 21# chromosomes, 6 18# chromosomes, 6 13# chromosomes and 3 X chromosomes and amplification detection of four amelo; all sites can be amplified by a quantitative fluorescent PCR (QF-PCR) technology, so as to detect the abnormal number of 21, 18, 13, X and Y chromosomal aneuploid. According to the kit, detection cycle is short, so the result can be obtained within 5 hours after obtaining the sample; the sensitivity is high; DNA at nanogram level, which is equal to DNA contained in 100 cells, is needed for each detection, so few samples are needed; 1 to 2ml of amniotic fluid can be collected to meet the demand; due to high sensitivity, a chimera containing more than 10% of trisomies can be detected.

The present invention provides methods for non-invasive determination of sex in a fetus or of Y chromosomal frequency abnormalities—indicative of aneuploidy or sex mosaicisms in a fetus—by detecting and determining the relative contribution genetic sequences from the Y chromosome in view of the percent fetal contribution in a maternal mixed sample.

The present invention relates to the technical field of sex identification, in particular to a method for sex identification of mammalian embryos. The present invention accurately identifies the sex of mammalian embryos by inserting a fluorescent reporter gene on the Y chromosome and through the fluorescent expression of the fluorescent reporter gene. Simple, efficient, accurate, and less damage; in the process of constructing a male mammalian model with a fluorescent reporter gene on the Y chromosome, the spacer region located in the Ddx3y and Uty genes is selected as the targeting region for the integration of exogenous genes, reducing the number of gene targeting sites The impact of the selection on the transgenic animal itself; in the gene editing process, the Cas9 protein and the single-guided sgRNA designed according to the gene sequence of the target region can be used to accurately cut the DNA of the target site and target the Ddx3y and Uty genes on the Y chromosome A fluorescent reporter gene is inserted between them, making the gene knock-in method efficient and simple.

Provided are an androgenetic haploid stem cell line, preparation method and use thereof. Specifically, provided are an androgenetic haploid cell and androgenetic blastula. The nucleus of the cell or the blastula only comprises a haploid autosome and a sex chromosome, the sex chromosome being the X chromosome and containing no Y chromosome. The androgenetic haploid cell of the present application can replace a spermatid as a ligand to generate a fertile animal individual, thus facilitating gene manipulation and being capable of transferring genetic information to the offspring.