Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular marker for identifying genetic sex of micropterus salmoides and primer pair thereof

A technique for largemouth bass and molecular markers, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problem of distinguishing between female fish and male fish, no difference, and no genetic identification from the perspective of cytology Issues such as gender, to achieve the effect of improving breeding efficiency and increasing economic benefits

Pending Publication Date: 2022-07-08
JIMEI UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Before the gonads of largemouth bass mature, the females and males of largemouth bass are difficult to distinguish from the external morphology. There is no difference in the morphology of male and female fish in the embryonic and larval stages; genetic sex; therefore, the development of a molecular marker that can identify its genetic sex for sex-controlled breeding of largemouth bass has great production application value

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker for identifying genetic sex of micropterus salmoides and primer pair thereof
  • Molecular marker for identifying genetic sex of micropterus salmoides and primer pair thereof
  • Molecular marker for identifying genetic sex of micropterus salmoides and primer pair thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Sex identification of largemouth bass from different populations

[0029] Cut part of the fin rays of the largemouth bass to be tested, put them in a 95% alcohol solution, and store them at -20°C for extracting genomic DNA to identify their genetic sex. The gender identification results were determined by the size of the amplified bands; the accuracy of the determination results was confirmed by histological observation after dissection. The gender of the samples used in the experiment has been confirmed by anatomical and histological observation;

[0030] Sources of template largemouth bass: 16 samples from Zhangzhou population, 16 samples from Putian population, 15 samples from Xiamen population, 14 samples from Nanchang population, and 14 samples from Foshan population. The largemouth bass individuals used were all 12-month-old adults.

[0031] Use a commercially available DNA extraction kit to extract genomic DNA, and the obtained genomic DNA is uniform...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a molecular marker for identifying genetic sex of micropterus salmoides and a primer pair thereof. The sequence of the molecular marker is shown as SEQ ID NO: 1, and the molecular marker exists on a genetic sex X chromosome of micropterus salmoides and is deleted on a Y chromosome. The sequences of the primer pair are shown as SEQ ID NO: 2 and SEQ ID NO: 3. The primer pair can be used for simply, quickly and stably identifying the genetic sex of different individuals in each population of the micropterus salmoides, including embryos, larvae and adults of the micropterus salmoides. And the accuracy reaches 100%.

Description

technical field [0001] The invention relates to the field of fish genome, in particular to a molecular marker for identifying the genetic sex of largemouth bass and a primer pair thereof. Background technique [0002] Sex determination is a biological process in which bisexual reproductive organisms determine the direction of sex development, and is a key link in life reproduction, directly related to the occurrence, survival, adaptation and evolution of species. Therefore, the mechanism of sex determination has always been a hot topic in life science research. In the vertebrate evolution system, fish is in a key position to link the past and the future, and because of its wide distribution, rich species, and diverse sex determination mechanisms, fish are important targets for research on sex determination mechanisms. Many fishes have sex differences in growth rate. Therefore, developing molecular markers that can accurately identify their genetic sex for these fish species...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6879C12Q1/6858C12N15/11
CPCC12Q1/6879C12Q1/6858C12Q2600/156C12Q2600/124C12Q2531/113C12Q2565/125Y02A40/81
Inventor 李完波何奇伟叶坤
Owner JIMEI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com