Reagent and kit for detecting sexual accompanying hereditary disease of fetus

Abstract

The invention discloses a reagent and a kit for detecting sex-associated hereditary diseases of fetuses. The reagent for detecting the sex-associated hereditary disease of the fetus comprises a Y chromosome specific primer and a Y chromosome specific probe, an upstream primer of the Y chromosome specific primer is Seq ID No.1, a downstream primer of the Y chromosome specific primer is a sequence shown as Seq ID No.2, and the Y chromosome specific probe is a probe with a fluorescent group at the 5'end and a quenching group at the 3 'end of a sequence shown as Seq ID No.3. According to the reagent for detecting the sex-associated hereditary disease of the fetus, the sex of the fetus can be judged by detecting blood of a pregnant woman, and the sex-associated hereditary disease is detected; the sex-associated hereditary disease related to the gender of the fetus can be found as soon as possible in the early pregnancy stage, and reference is provided for related treatment.

Description

technical field [0001] The invention relates to the field of early fetal detection, in particular to a reagent and kit for detecting fetal sex-associated genetic diseases. Background technique [0002] Fetal sex-linked genetic diseases refer to genetic diseases related to fetal sex, such as congenital agammaglobulinemia, X-linked cerebral edema, or mental retardation. Among them, X-chromosome recessive inheritance and Y-chromosome inheritance can be directly judged by detecting the sex of the fetus. [0003] However, the traditional method of fetal gender detection is mainly through four-dimensional or color Doppler ultrasound. Clinically, the fetus needs to grow to a certain extent before it can be accurately judged, which is extremely unfavorable to pregnant women. Moreover, the accuracy rate of judging the sex of the fetus by four-dimensional or color Doppler ultrasound is only about 85%, which is not conducive to the detection of fetal sex-linked genetic diseases. [0...

AI Technical Summary

Problems solved by technology

[0003] However, the traditional method of fetal gender detection is mainly through four-dimensional or color Doppler ultrasound. Clinically, the fetus needs to grow to a certain extent before it can be accurately judged, which is extremely unfavorable to pregnant women.

Moreover, the accuracy rate of judging the sex of the fetus by four-dimensional or color Doppler ultrasound is only about 85%, which is not conducive to the detection of fetal sex-linked genetic diseases

[0004] In addition, invasive testing methods such as amniocentesis can also be used to detect fetal gender; however, invasive testing is generally avoided in the process of prenatal testing, after all, invasive testing has potential safety hazards

Especially for some special groups, such as women over 40 years old, women with a history of multiple miscarriages, etc., the risk of invasive testing will be greatly increased

[0005] More importantly, it is difficult for the existing fetal gender detection methods or fetal sex-linked genetic disease diagnostic techniques to perform accurate and effective detection in the first trimester, that is, before 12 weeks of pregnancy.