The invention provides a detection method of genes in
chromosome 21. The method comprises the steps as follows:
genomic DNA (deoxyribonucleic acid) of a detected object is extracted; two sets of PCR (
polymerase chain reaction) primer pairs and
Taqman MGB (
minor groove binder)
fluorescence probes are combined to perform fluorescent quantitative PCR on the
genomic DNA respectively, wherein the PCR primer pairs are
nucleotide sequences represented by SEQ ID NO: 4 and SEQ ID NO: 5 as well as SEQ ID NO: 7 and SEQ ID NO: 8 respectively, and
nucleotide sequences of the
Taqman MGB
fluorescence probes are represented by SEQ ID NO: 6 and SEQ ID NO: 9 respectively; and according to a real-time amplification curve formed by
fluorescence signals collected in the fluorescent quantitative PCR, whether
trisomy probability exists in the
chromosome 21 of
genomic DNA is analyzed. The invention further relates to correlated detection probe combination, a detection kit and a correlated application. According to the invention, the design is ingenious,
sample contamination can be effectively prevented through
closed tube detection, the detection is rapid, accurate, simple and convenient, and the method can be taken as reference for doctor diagnosis and medication and is suitable for large-scale popularization and application.