157 results about "Gestation" patented technology

A method for determining the volume of an irregularly shaped body part or body such as a fetus using a 3D ultrasonic image composed of light and dark pixels. A caliper is placed on a portion whose volume is to be determined and a computer having the image, automatically counts every pixel with the same echogenicity in every direction as long as it is continuous with the spot the caliper is in, and wherein the computer means stops counting in any direction where the pixels become significantly more echogenic (dark). The volume of the portion, is determined, based on the counted number of pixels and the magnification of the image; and the calculated volume is used to determine physiological characteristics based on known values such as fetal gestation age.

The invention discloses a special gestation compound feed for sows at later stage of pregnancy. The special gestation compound feed is prepared from the following raw materials: corn, bran, rice bran meal, DDGS (dried distiller grains with soluble), swelling soya dregs, swelling soybean flour, fermented soybean meal, compound sugar, mountain flour, calcium hydrogen phosphate, choline chloride, common salt, compound premix, L-lysine hydrochloride, L-threonine, acidifier, clostridium butyricum and yeast cell wall polysaccharides. The invention, starts from nutrition of the sows at the later stage of the pregnancy, gives consideration to the growth of fetuses and the physiological characteristics of the sows per se, designs daily ration with high protein, high amino acid and low energy, adds the compound sugar constituted by different monosaccharides and disaccharides, and can provide energy which can be utilized quickly and improve the birth weight and the uniformity of piglets; and by adding the clostridium butyricum and the yeast cell wall polysaccharides, the special gestation compound feed can improve the intestinal health of the sows, improve immunity, regulate intestinal peristalsis, reduce the constipation incidence of the sows, shorten the labor stage of the sows, reduce the incidence rate of mastitis and endometritis of the sows, and assist the sows in rapid postpartum recovery.

A sow management system makes use of separate Trickle Feeding and loafing areas separated by a one way gate or gates to establish a general continuous flow of sows through an interrogator apparatus where an RFID tag for each sow is read to determine if such sow should be returned to the general Trickle Feeding area or at least two other possible areas through automatic gating controlled by computer means sensitive to RFID tagging of the sows.

The present invention provides improved vaccination methods for increased protection against ileitis. The methods provide for the vaccination of young animals, preferably piglets, between 10 and 26 days of age, vaccination of pregnant sows during the second or third stages of gestation, and a combination of these methods. Vaccination of the pregnant sows can occur using repeated and / or high doses of Lawsonia antigen prior to farrowing.

The present invention concerns a global maternal serum proteome map and its changes during healthy gestation. Accordingly, the present invention provides an important tool for plasma-based maternal-fetal diagnostics.

The invention relates to developments in the treatment of diabetes in mammals. Particularly it relates to a method of preparing a xenotransplantable porcine islet preparation capable upon xenotransplantation of producing porcine insulin in an appropriate recipient mammal, the method including or comprising the steps of: (I) harvesting the pancreas of piglets at or near full term gestation, and (ii) extracting pancreatic beta islet cells from the harvested pancreas wherein the islets (at least at some stage in the performance of the method) are exposed to nicotinamide. Further, the invention relates to a method of encapsulation of a xenotransplantable porcine islet preparation, and transplantation of such a preparation, or a capsule containing such a preparation, into an appropriate recipient mammal.

The invention provides a constipation-resisting complete formula feed for sows at the latter half of gestation. The complete formula feed is prepared from the following components: 19%-21% of corn, 10%-12% of soybean meal, 1.9%-2.5% of soybean peel, 28%-32% of wheat, 8%-12% of alfalfa powder, 13%-18% of barley, 1.5%-1.8% of soybean oil, 3%-8% of rice bran, 0.4%-0.7% of L-lysine sulfate, 0.20%-0.40% of L-threonine, 0.15%-0.20% of glycine betaine, 1.5%-2.0% of calcium hydrophosphate, 0.85%-1% of calcium carbonate, 0.01%-0.05% of sodium butyrate, 0.3%-0.5% of table salt, 0.5% of a vitamin premix, 0.3% of a microelement premix, 0.03% of a compound enzyme preparation, 0.02% of high-temperature-resisting phytase and the balance being other auxiliary additives. According to the constipation-resisting complete formula feed, the digestible rate of the feed can be increased and the digestion load of intestinal tracts is reduced; and high-quality fiber components and micro-ecological preparations are added so that the excessive absorption of water, caused by the fact that the digestion function is reduced, is avoided and the constipation of the sows at the latter half of gestation can be reduced.

The invention relates to a traditional Chinese medicine preparation for promoting lactation of pregnant sows, which is composed of astragalus, angelica, Codonopsis pilosula, Rhizoma Chuanxiong, Wang Buliuxing, Passepartout, motherwort and Cyperus cyperi. Significantly increase the serum prolactin (PRL) and growth hormone (GH) levels of lactating sows, promote the lactation performance of sows; increase the protein content in colostrum, promote the growth and development of sucking piglets; increase the serum complement C3 and immunoglobulin of sucking piglets (IgG, IgM, IgA) content and superoxidase (SOD) activity, reduce malondialdehyde (MDA) content, improve immune function and antioxidant function of suckling piglets.

The invention discloses a pregnant sow compound feed capable of improving the vitality of firstborn piglets and an application method of the feed. The feed is prepared from the following components in percentage by weight: 45-55% of corn, 5-10% of bean pulp, 9-14% of bran, 3-8% of rice bran, 12-18% of DDGS, 1.5-2.5% of coconut oil, 1.0-1.3% of calcium hydrophosphate, 0.8-1.2% of mountain flour, 0.1-0.25% of choline chloride, 0.3-0.4% of salt and 1-2% of premix. The pregnant sow compound feed is capable of improving the development level and the health condition of fetuses by regulating the nutrition for sows in the latter half of gestation, and therefore, the birth weight, the uniformity and the survival rate at the early stage of lactation of firstborn piglets can be effectively improved; as a result, the vitality of the firstborn piglets can be effectively improved.

The invention provides a gestation diabetes risk monitoring system based on dynamic physics and physical and chemical factors and belongs to the field of medical instruments in the department of obstetrics and gynecology. The gestation diabetes risk monitoring system is characterized by comprising a computer, a toggle switch array, a resistor array and a gestation diabetes pathogenesis risk LED alarming module; a pregnant women information input module of dynamic physics check and physical and chemical factors, a logic switch array module of pregnant women gestation diabetes pathogenesis risk factors and logic regression value computation module of pregnant women gestation diabetes pathogenesis risk are arranged in the computer; a voltage comparison module and an LED array are arranged in the gestation diabetes pathogenesis risk LED alarming module. According to the invention, the logic switch value is utilized to control the make-and-break state of the toggle switch; a risk factor value in the logic regression manner is utilized to control the resistance value of the resistor array, so as to indicate the relative risk degree; the resistance value of the resistor array is utilized to control a voltage comparator, so as to output electrical level and light the LED alarm. According to the invention, the risk degree of the dynamic gestation diabetes can be comprehensively evaluated from multivariate physical and chemical factors and the real-time alarm can be conducted.

The invention relates to a method of detecting chromosomal abnormalities, in particular, the invention relates to the diagnosis of fetal chromosomal abnormalities such as trisomy 21 (Down's syndrome) which comprises sequence analysis of cell-free DNA molecules in plasma samples obtained from maternal blood during gestation of the fetus.

The present invention provides a management method for quantitatively feeding sows based on backfat, which is carried out step by step according to the following stages: the first stage: after the sow is weaned, the second stage: 1-30 days of pregnancy after mating, the third stage: 31-31 days of pregnancy 85 days, the fourth stage: after 85 days of gestation and the fifth stage: after giving birth. Adopting the management method of the present invention to raise sows can adjust the body condition of individual sows to the best state, so that the overall pig population is uniform and healthy, and feeding with evidence can reduce the waste of feed caused by blind feeding, and promote the development of newborn piglets. The weight of the sow is uniform and reasonable, which promotes the maximum productivity of sows and prolongs the reasonable service life of sows.

Methods, systems and computer program products for estimating the gestational age of a fetus are provided. According to one embodiment, the method generates a component image from a segmented ultrasound image of a fetal head. The component image includes one or more edges. The method then identifies a third ventricle within the component image. The method estimates a length of a bi-parietal diameter, based at least in part on the orientation of the third ventricle. Thereafter, the method estimates the gestational age of the fetus.

The invention provides a novel sow feeding method. According to the method, each sow is divided into four different physiological stages including the first half period of gestation, the latter half period of gestation, the suckling period and the weaning estrualization stage, and corresponding fodder is fed at each stage in a matched mode. By the adoption of the method, the problems of sow constipation, poor mammogenesis, great weight loss after weaning and long dioestrus are effectively solved, the problems that the birth weight of piglets is small, uniformity is poor, the weaning weight is low and the rate of survival is not high are also solved, and thus the rate of survival of the piglets at the nursing period and the use efficiency of the sows can be better improved.

A device for interrupting the gestating cycle of a mosquito includes a container having a bottom wall and side walls. A generally porous sheet of material is placed over the upper opening thus formed in the container by securing it to the periphery of the container. Double sided tape, mechanical clips, etc. may be used to attach the porous material to the periphery of the container to cover the open area. The porous sheet of material is farametis and contains apertures that are small enough to prevent an adult mosquito from passing through it but yet allow evaporation of water placed into the container and water molecules through the apertures. A sticky substance is placed on the outer surface of the porous sheet. Water is evaporated from the container and water molecules are released through the apertures into the atmosphere above the porous sheet. Gestating mosquito's are attracted to the device and attempt to enter the container to lay there eggs in the water within the container but can not. Instead, the mosquitoes are immobilized on the surface of the porous material when retained by contact with a sticky substance and prevent it from laying eggs in the container or any other body of water. This breaks the reproductive cycle of the mosquito.

The invention discloses a method for breeding transgenic livestock rich in polyunsaturated fatty acid. The method comprises the following steps of: guiding DNA molecules which have a sequence 1 and a sequence 2 in a sequence list into somatic cells of a mammal to obtain transgenic cells in which a delta-12 fatty acid dehydrogenase and an omega-3 fatty acid dehydrogenase are expressed; cloning embryos by using the transgenic cells as nuclear transfer donor cells and using isolated ovocytes as nuclear transfer acceptor cells by nuclear transfer technology; and transplanting the cloned embryos into a uterus of the livestock by a non-operation method for gestation to obtain the transgenic livestock. The method of the invention can ensure that the delta-12 fatty acid dehydrogenase and the omega-3 fatty acid dehydrogenase are stably expressed in the body of a transgenic pig and that the content of omega-3 polyunsaturated fatty acids (PUFAs) in meat of the livestock is improved obviously.

The invention provides a kit and method for detecting mutation of a thalassemia-related gene and a use thereof and relates to the technical field of medical genetics. The kit for detection is used for detecting many types of patients such as people needing premarital checkup, people in gestation, newborns, people in a zone having high incidence of thalassemia and people having thalassemia family heredity history, can realize accurate, comprehensive, visual and simple detection of mutation of a thalassemia-related gene, has a detection mutation range comprising HBA1, HBA2 and HBB genes in the genome and neighbouring zones and has the characteristics of simpleness, accuracy, good repeatability and promotion and use easiness.

Disclosed herein are processes for enhancing ability to learn in a puppy or kitten comprising orally administering to the puppy or kitten a composition comprising an essential fatty acid component comprising a therapeutically effective amount of docosahexaenoic acid. Further disclosed are processes for enhancing ability to learn in a puppy or kitten comprising orally administering to a respective maternal animal a composition comprising an essential fatty acid component comprising a therapeutically effective amount of docosahexaenoic acid during gestation, nursing, or weaning of the puppy or kitten. Further disclosed are kits comprising a composition comprising an essential fatty acid component comprising a therapeutically effective amount of docosahexaenoic acid; and information that use of the composition will enhance ability to learn in a puppy or kitten.

The invention provides a CD28 gene overexpression vector and application thereof. A target biological genome serves as a template, left and right homologous arms of a first intron of an ROSA26 gene, a CD28 gene expression frame and an OCT4 gene promoter are amplified through PCR (polymerase chain reaction), and the left homologous arm, the CD28 gene expression frame, LoxP-locus-containing Cre expression frame and Neo gene expression frame both regulated by the OCT4 gene promoter, the right homologous arm and a negative selection marker DTA are connected sequentially and are constructed to an eukaryotic expression vector to obtain the overexpression vector. The overexpression vector and a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas (CRISPR-associated) 9 target vector containing a first intron of a specific target pig ROSA26 gene are transplanted into a fibroblast of a pig fetus, a positive cell serves as a donor cell, an oocyte serves as a recipient cell, and a cloned embryo is obtained according to a somatic cell nuclear transfer technology; the cloned embryo is transplanted into the uterus of a pig for gestation to obtain a transgenic pig with a CD28 gene which is integrated at a fixed point in the first intron of the ROSA26 gene, wherein a marker gene of the transgenic pig is knocked out automatically.

The invention relates to application of colchicin in preparing drugs, in particular to application of colchicin in pharmacotherapy of cholestatic liver disease, the application of colchicin in preparing a cholestatic liver disease drug, and the application of colchicin in preparing the drug for treating gestation intrahepatic cholestasis syndrome, inner primary cirrhose biliaire, primary sclerosing cholangitis severe jaundice or immunity jaundice cholestatic liver disease. The colchicin can effectively lower liver injury and cholestasis caused by cholestatic liver disease, i.e. the colchicin can effectively treat cholestatic liver disease. The colchicin can effectively lighten hepatic cell necrosis, inflammatory cell infiltration and bile capillary hyperplasia caused by cholestasis. The colchicin can effectively increase bile acid, bilirubin and expression of phospholipid transporters BSEP, MRP2 and MDR3 in a bile duct, accelerates excretion of bile acid, bilirubin and phospholipid deposited in the body and reduces liver damage.

The invention discloses a nutritive feed additive capable of increasing the survival ratio of embryos in the first half period of cow gestation. The nutritive feed additive comprises the following components in parts by weight: 1-5 parts of organic selenium, 5-15 parts of beta-carotene, 5-15 parts of vitamin C and 20-50 parts of an immune enhancer. The nutritive feed additive disclosed by the invention is capable of eliminating free radicals in sow placentas, maintaining the stability of environments in uteruses, improving resistance to disease and immunity of sows and embryos and achieving the purposes of increasing the survival ratio of the embryos and born number.

The invention relates to a sow feed additive for improving the weaning weight of a piglet and an application scheme of the sow feed additive, belonging to the field of animal feeds and being applied to the industry of livestock breeding. The sow feed additive for improving the weaning weight comprises main ingredients of 5-20 percent of chitosan oligosaccharide and the balance of maltodextrin. The application scheme of the sow feed additive for improving the weaning weight of the piglet specifically comprises the step: at a start of 80th-90th days of gestation of the piglet, after adding the sow feed additive in a diet of a sow, feeding the sow until the lactation is ended. In the application scheme, the addition amount of the sow feed additive is specifically as follows: 200-500g of the sow feed additive is added in each ton of the diet of the sow. The sow feed additive disclosed by the invention is simple in use method, and remarkable in weaning weight of the piglet.

The invention discloses a pSP107 plasmid as well as application and a construction method thereof, and belongs to the field of biotechnology. The invention provides a novel plasmid, pSP107 plasmid, aswell as the application and the construction method of the pSP107 plasmid. The plasmid provided by the invention can be transformed into protoplasts of UET2 and UEMT2 to obtain a UeTSP strain and a UeMTSP strain; and the two strains can infect zizania latifolia and cause zizania latifolia to be pregnant, thereby providing a new idea for artificial zizania latifolia gestation.

The invention discloses a method for efficiently separating and purifying mammary epithelial cells. The method is suitable for a plurality of mammals. The mammary tissues of adult mammals, which are in the middle and later periods of gestation or in the lactation period, are collected through aseptic operation. According to the provided method, the separation and purification can be achieved in one step, the whole experiment is simple and efficient; the purification, which comprises repeated differential digestion and differential attachment, in the conventional method is eliminated, the purity of mammary epithelial cells obtained by the provided method can reach 98% or more, the obtained mammary epithelial cells can be continuously cultured in-vitro and passed down for 40 generations, and the lactating function of mammary epithelial cells is not affected. The obtained mammary epithelial cells can be applied to the researches on growth of mammary gland, lactating mechanism, mammary bioreactor, transgenic animals, mammary diseases, and the like.

The invention provides a method for separating and culturing human umbilical cord mesenchymal stem cells, which comprises the following steps: taking umbilical cord of fetus born after the normal period of gestation by caesarean section, flushing by PBS under aseptic condition, removing the bloodstain; using an aseptic scissor in an animal cell medium to cut the washed umbilical cord to tissue blocks, removing blood vessel and crushing, then fully mixing with the animal cell medium and oscillating, centrifuging, mixing the centrifuged deposition and a MesenCult-XF complete medium and oscillating, and uniformly planting in a cell culture dish, placing in an incubator with the temperature of 37 DEG C and 5% of CO2 for culturing for 5 days, sucking the tissue blocks out and discharging, refilling the MesenCult-XF complete medium, and replacing the medium every three days for continuously culturing to obtain the umbilical cord mesenchymal stem cells. According to the invention, the animal serum is used, the operation is simple, the repeatability is good, the security is high, and the obtained cell enables stabilization through multitime passage.

A catheter with a guide wire and handle is provided for sampling an area of interest, such as chorionic villi. The catheter contains a cannula of flexible plastic that is non-pyrogenic and latex-free, and a flexible obturator with an elongate cylindrical handle. The cannula is echogenic and can be easily visualized with visualization or imaging device. In an embodiment, the catheter may be curved manually by the operator to conform to the patient's anatomy and it is inserted through the cervix into a pregnant uterus at 10-13 weeks gestation and placed into the developing placenta under ultrasound guidance and a guide mechanism provided on the handle. In this embodiment, once the catheter has been placed, the obturator is withdrawn and a sample of chorionic villi is removed by applying appropriate pressure to the proximal end of the cannula and slowly withdrawing the cannula from the uterine cavity.

The invention discloses a method for improving synchronous mating of seahorses. Due to adjustment on comprehensive factors, such as parent screening (a plurality of seahorses parental populations with a consistent sexual maturation period are screened and separated out according to the sexual maturation degree), domestication and breeding equipment improvement and parental physiological characteristic observation, in a seahorse breeding process, parent seahorses can be promoted to form a stable periodic reproduction for mating, gestation, parturition and remating, so that the parturition rate in the reproduction peak period (a single week) is over 60 percent, and the reproduction efficiency of the seahorses can be effectively improved by over 10 percent.

A device for interrupting the gestating cycle of a mosquito includes a container having a bottom wall and side walls. A generally porous sheet of material is placed over the upper opening thus formed in the container by securing it to the periphery of the container. Double sided tape, mechanical clips, etc. may be used to attach the porous material to the periphery of the container to cover the open area. The porous sheet of material is farametis and contains apertures that are small enough to prevent an adult mosquito from passing through it but yet allow evaporation of water placed into the container and water molecules through the apertures. A sticky substance is placed on the outer surface of the porous sheet. Water is evaporated from the container and water molecules are released through the apertures into the atmosphere above the porous sheet. Gestating mosquito's are attracted to the device and attempt to enter the container to lay there eggs in the water within the container but can not. Instead, the mosquitoes are immobilized on the surface of the porous material when retained by contact with a sticky substance and prevent it from laying eggs in the container or any other body of water. This breaks the reproductive cycle of the mosquito.

The invention provides a feed additive for improving the immunoglobulin content in sow colostrum. The feed additive comprises the following ingredients by weight percent: 16.7-32.3% of calcium chloride, 3.3-17.7% of ammonium chloride, 16.7-33.3% of citric acid, 0.7-2% of magnesium sulfate, 0.1-0.3% of ligustrum lucidum polysaccharide, 0.3-0.7% of saccharin sodium salt, 1.0-2.0% of powdered rice hull, and 11.7-61.2% of zeolite powder. The additive can improve the immunoglobulin content in the serum of a sow in latter half of gestation and the immunoglobulin content in post-partum colostrum to further improve the immunity of a piglet in suckling period and weaning period and reduce piglet diarrhea.

The invention discloses the semifluid extract of deer foetus, which takes the deer foetus, deer born after the normal period of gestation and not fed by breast milk, American ginseng, medlar, eucommia bark, white paeony root, spina date seed, villous amomum fruit, safflower, echinacea, astragalus and honey as raw materials. The semifluid extract of deer foetus is prepared by the following steps of: pre-treating the deer foetus and the deer born after the normal period of gestation and not fed by the breast milk, preparing deer foetus powder and the powder of the deer born after the normal period of gestation and not fed by the breast milk from the deer foetus and the deer born after the normal period of gestation and not fed by the breast milk by adopting freeze-drying technology, compounding the deer foetus powder, the powder of the deer and the other components according to proportion, and performing bulking and sterilization to prepare the semifluid extract. The semifluid extract of deer foetus completely maintains the bioactive ingredients of the deer foetus and the deer born after the normal period of gestation and not fed by the breast milk, has the double effects of treatment and nourishing, and is particularly suitable to be taken by weak patients with immune dysfunction, female chronic pelvic inflammatory disease, leucopenia after cancer chemotherapy and radiotherapy, and the like.