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37 results about "Cel" patented technology

A cel, short for celluloid, is a transparent sheet on which objects are drawn or painted for traditional, hand-drawn animation. Actual celluloid (consisting of cellulose nitrate and camphor) was used during the first half of the 20th century, but since it was flammable and dimensionally unstable it was largely replaced by cellulose acetate. With the advent of computer-assisted animation production, the use of cels has been all but abandoned in major productions. Disney studios stopped using cels in 1990 when Computer Animation Production System (CAPS) replaced this element in their animation process, and in the next decade and a half, the other major animation studios phased cels out as well.

Reversible photobleachable materials based on nano-sized semiconductor particles and their optical applications

Semiconductor nano-particles, due to their specific physical properties, can be used as reversible photo-bleachable materials for a wide spectrum, from far infrared to deep UV. Applications include, reversible contrast enhancement layer (R-CEL) in optical lithography, lithography mask inspection and writing and optical storage technologies.
Owner:PIXELLIGENT TECH LLC

Static Memory Device with Five Transistors and Operating Method

At the bottom of a column (COLi) of memory cells (CEL) of the SRAM type with five portless transistors, there is placed an additional cell (CLS), with a structure identical to the cells (CEL), which makes it possible to write and read a datum in a memory cell (CEL) of the column without using a read amplifier.
Owner:STMICROELECTRONICS SRL

Method for evaluating influence of self-elevating drilling ship pile insertion on adjacent jacket platform pile foundation

The invention relates to a method for evaluating influence of self-elevating drilling ship pile pitching on an adjacent jacket platform pile foundation. The method comprises the steps of 1, determining the uniform equivalent elasticity modulus of a soil layer on one side of a jacket pile; 2, building a three dimensional structure analysis model and a three dimensional elastic-plastic finite element CEL (Couple Eulerian-Lagrangian) model and setting an elasticity modulus; 3, determining angles of environmental parameters; 4, determining UC value distribution of a pile body, pile head force and pile head displacement data under the environmental load effect; 5, determining the pile body displacement of the jacket pile foundation under the pile pitching squeezing effect; 6, building a pile soil non-linear footing beam module and calculating pile body internal force; 7, programming under MS EXCEL according to design codes in oceaneering to obtain the UC value distribution of the pile body under the action of the squeezing effect; 8, determining the UC value of the pile body under the coupling of the environmental load effect and the squeezing effect; 9, checking the UC value of the pile body under the coupling condition; 10, analyzing the jacket pile foundation; 11, checking the analysis result and judging whether the design requirement is met or not.
Owner:CHINA NAT OFFSHORE OIL CORP +1

Biomarkers for the detection and screening of down syndrome

The disclosure includes assays and methods for screening for risk of Down syndrome and / or trisomy 21 in a fetus. The assays and methods comprise determining the level of at least one biomarker selected from mucin 13 (MUC13), bile salt-activated lipase (CEL), dipeptidyl peptidase 4 (DPP4), carboxypeptidase A1 (CPA1), amyloid precursor protein (APP) and tenascin-C (TNC-C) polypeptides in a test biological sample from a pregnant subject, wherein a decreased level of MUC13, CEL, DPP4, and / or CPA1 polypeptide and / or an increased level of APP and / or TNC-C polypeptide in the test biological sample compared to a corresponding reference biomarker polypeptide level indicates an increased risk of Down syndrome or trisomy 21 in the fetus. The disclosure also includes assays, compositions, immunoassays, and kits for performing the methods disclosed herein.
Owner:UNIV HEALTH NETWORK

Image generation system and program

The present invention provides an image generation system and program that apply shading for producing a cel animation type of image, with a reduced processing load. Brightness information is set for each pixel of a two-dimensional image that is obtained by perspective transformation of a three-dimensional object, based on brightness information set for that three-dimensional object, the two-dimensional image is divided into a plurality of areas based on the thus-set brightness information, and stepped shading is applied in units of the thus-divided plurality of areas. This brightness information could be set beforehand or it could be calculated in real-time, based on a given condition. The system could also comprise means for determining the pixels to be rendered by comparing the brightness information of each pixel with a given threshold value, then performing adjustment processing on the color information of the pixels to be rendered, based on color information that acts as a reference. This threshold value can be varied in accordance with the number of steps of shading, and adjustment processing can be performed a plurality of times on color information of pixels to be rendered.
Owner:BANDAI NAMCO ENTERTAINMENT INC

Methods for Improving Diabetes Management

A method for determining the levels of biomarkers, specifically, advanced glycation end products (AGEs) and oxidation products (Ops) in a biological sample such as a plasma ultrafiltrate, is used to determine a patient's risk and / or rate of developing diabetes related nephropathy. The preferred biomarkers to measure include Nε-(1-carboxyethyl-lysine (CEL), methylglyoxyl-derived hydroimidazolone (MGHI) and Nε-carboxymethyllysine (CML). Also provided herein is a method of diabetic care which includes determining a diabetic patient's risk of developing diabetes related kidney disease and adjusting the patient's treatment regimen to include in addition to glucose lowering agents, additional treatments such as medications that modify the renin-angiotensin system, or specialized diets with low levels of AGEs or oxidative products.
Owner:PREVENTAGE HEALTHCARE

Wrapped solar cel

A photovoltaic device comprising a photovoltaic cell and at least one layer, the photovoltaic ceil and at least one layer wrapped from the inside out to form the photovoltaic device having a vertical geometry is provided. The photovoltaic device can be a variety of shapes. These shapes include a cylinder, square, oval, rope, ribbon, oblong and rectangular. Generally, the photovoltaic cell has at least on semiconductor, a hirfi work-function electrode and a low work-function electrode.
Owner:CURRAN SEAMUS

A breaker device for interrupting current on a transmission line

A breaker device for interrupting current flowing on a transmission line, the device comprising three electrical branches (B1, B2, B3) connected in parallel, including a main branch (B1) in which the current to be interrupted flows and an auxiliary branch (B2), the main branch comprising at least one semi-conductor breaker cell (CEL1) connected in series with at least one mechanical interrupter / disconnector (Sm), the auxiliary branch (B2) comprising at least one thyristor, the breaker device further comprising a control circuit (CM) suitable for interrupting a current that flows in the main branch. Once the current is interrupted in the semi-conductor element of the breaker cell of the main branch, the control circuit acts to command the thyristor of the auxiliary branch (B2) to be put into a conductive state whenever a current flowing in the voltage limiter(s) reaches the value of the current that is flowing in the transmission line.
Owner:GENERAL ELECTRIC TECH GMBH

Expansion tank for vehicle cooling system

An expansion tank (10) for a vehicle cooling system (18) of an engine using a liquid coolant (16) includes a tank body (12) defining a first volume (V1) containing coolant (16), wherein the coolant defines a variable coolant elevation level (CEL) within the tank body. The tank body (12) also defines an upper volume (20) containing air. A bladder (14) is disposed in the tank body (12) and defines a second volume (V2) containing air. The bladder (14) includes a flexible membrane (36) actuated by an actuator (46). When the engine is stopped or is below a predetermined temperature, the flexible membrane (36) is moveable to a first position (FP) which lowers the coolant elevation level (CEL), and when the engine is started or reaches a predetermined temperature, the flexible membrane (36) is moveable to a second position (SP) which raises the coolant elevation level. A communicating line (38) is in fluid communication between the upper volume (20) and the second volume (V2) to fluidly communicate air therebetween.
Owner:INT ENGINE INTPROP CO LLC

Ceria-doped rare-earth phosphate luminescent material and preparation method thereof

The invention belongs to the technical field of rare-earth phosphate luminescent materials, in particular relates to a rare-earth phosphate luminescent material using ceria as an activating element and a preparation method thereof. The luminescent material comprises components shown as XxXy'Cel-x-Ypo4, wherein X and X' are one of rare-earth elements La, Y and Gd; x is more than or equal to 0 and less than 1; and y is more than and equal to 0 and less than 1. The preparation method comprises the following steps of: precipitating and aging rare-earth precursor solution and diammonium hydrogen phosphate, mixing with a fluxing agent, sintering at high temperature, and reducing to obtain the luminescent material. The fluorescent powder can be excited by ultraviolet rays with the wavelength of between 400 and 500nm, and can emit peak wavelength of 417+ / -1nm, 423+ / -2nm, 425+ / -2nm and 456+ / -1nm along with changes of the components and concentration. The product can be used for an anti-counterfeiting device.
Owner:苏振彪

Method of hierarchical caching of configuration data having dataflow processors and modules having two-of multidimensional programmable cell structure (FPGAs, DPGAs, etc.)

Instead of integrating as previously a central and global unit in one module which processes all configuration requests, now there is a plurality of hierarchically (tree structure) arranged active units which can assume this task. A request from the lowest level (the leaves in the hierarchy) is only forwarded to the next higher level if the request could not be processed. These steps are repeated for all the levels present until the highest level is reached. The highest level is connected to an internal or external higher level configuration memory, which contains all the configuration data required for this program run. A type of caching of the configuration data is achieved due to the tree structure of the configuration units. Access to configurations mainly takes place locally. In the most unfavorable case, a configuration must be loaded from the higher level configuration memory if the respective data is not present in any of the hierarchically arranged CTs. Deadlocks are prevented by introducing a fixed time sequence of the configurations to be loaded and combining the configurations in a list. The status information of the CELs is saved before loading and thus remains unchanged during the processing of the entire list of configurations.
Owner:SCIENTIA SOL MENTIS AG +1

Method for extracting CEL I nuclease in celery

InactiveCN101538561ANo significant difference in purityNo significant difference in activityHydrolasesSulfite saltFiltration
The invention relates to a method for extracting CEL I nuclease in celery and discards measures adopting a plurality of high-speed or ultra-speed centrifugation and a plurality of gel filtration and ion exchange chromatography in the prior art. The method comprises the following steps: adding a small amount of sodium sulfite to extract so as to reduce and clear colored substances in the extract; adopting a thermal denaturation physical method to rapidly clear a great amount of foreign protein with poor temperature toleration; utilizing the characteristic that CEL I is the combination of glycoprotein and activated concanavalin to combine CEL I nuclease and concanavalin so as to remove the foreign protein and further purify the CEL I; and finally, selecting DEAE-Sepharose FF as a suitable medium for the CEL I by once chromatography purification. Accordingly, the extraction method replaces the time-consuming and expensive measures adopting a plurality of high-speed even ultra-speed refrigerated centrifugation, a plurality of gel filtration and ion exchange chromatography, and the like and achieves the aims that the CEL I nuclease is rapidly extracted from the celery and purified.
Owner:GUANGZHOU UNIVERSITY

Method for testing toxicity of high algae-laden aquatic organisms

InactiveCN105483202AFacile apoptosis rateSimple death ratePollution detectorsMicrobiological testing/measurementTesting toxicityPhosphate
The invention discloses a method for testing the toxicity of high algae-laden aquatic organisms. The method comprises the steps that saccharomyces cerevisiae is cultured in a solid medium containing 5% of YPD and 1.5% of agar firstly, after clone is grown out, a YPD fluid medium of 5% is inoculated with monoclone, and overnight shake cultivation is conducted based on 180-250 rpm and 25-58 DEG C; phosphate buffer is added to a culture solution with the OD value of 1.5-1.8 to obtain a blank sample A, a high algae-laden water sample is added to the culture solution with the OD value of 1.5-1.8 to obtain a detection sample B, and the two samples are placed on a mixer for oscillation lasting 2 h; cell washing is conducted on the sample A and the sample B twice by means of PBS, and cell suspension with the concentration of 1*105-1*106 cel ls / mL is prepared; the sample A and the sample B are both added to Annexin V-FITC of 5 mug / mL to be evenly mixed, and then propidium iodide of 5 mug / mL is added to be evenly mixed; reaction is conducted at the room temperature away from light for 5-15 min; flow cytometry detection is conducted within 1 h; the proportion of dead cells, the proportion of living cells and the proportion of apoptotic cells are detected by means of flow cytometry; the apoptosis rate and the death rate of the sample B are compared with those of the blank sample A, so that water toxicity is judged. Early warning technical support is provided for high algae-laden water pollution, so that high water supply quality is guaranteed.
Owner:上海艾耐基科技股份有限公司 +1

Hsa-miR-489 detection kit based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and detection method thereof

Ahsa-miR-489detection kit based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and a detection method of the hsa-miR-489detection kit relate to MicroRNA. The detection kit is provided with a kit body, a clapboard, anexogenous reference bottle, a stem-loop reverse transcription reagent bottle, and a real-time fluorescent quantitative PCR reagent bottle. The miRNA in a sample is extracted, and if the sample is a serum / plasma sample or other liquid samples, then 5 mu L exogenous reference cel-miR-39 with the concentration of 5nmol, provided by the reagent bottle, is added after the sample is fully split, and the mixture is oscillated in vortex for 15s; but if the sample is a cell or tissue sample, then exogenous reference cel-miR-39 is not added; a stem-loop reverse transcription reagent provided by the reagent bottle is used to reversely transcribemiRNA into cDNA; a real-time fluorescent quantitative PCR reagent provided by the reagent bottle is used to conduct real-time PCR amplification; various data provided by an analytical instrument are integrated together, and reasonable thresholds and reference lines are set for result analysis.
Owner:ZHONGSHAN HOSPITAL XIAMEN UNIV

Detection kit for chicken CEL gene promoter 99bp indel polymorphic marker and application of detection kit

The invention relates to a detection kit for a chicken CEL gene promoter 99bp indel polymorphic marker and application of the detection kit, and belongs to the technical field of molecular genetics breeding. According to the detection kit and the application, the CEL gene promoter 99bp indel polymorphic marker is found and can be used for assistant selection and molecular breeding of chickens; specifically, according to a sequence as shown in SEQ ID NO.1, a primer found to be without 183rd to 281st sites from the 5' end of the sequence is designed and amplified, whether the 183rd to 281st sites are lost or not is judged according to the size of the amplification product, then whether a detection sample is DD or II or ID gene type is judged, and the slaughter performance of local chickens is improved by enhancing breeding of DD gene type individuals. The application method established through the detection kit does not need digestion, is high in resolution, accurate in type judgment, easy to operate, low in cost and short in period, does not need special instruments, and is easy to popularize.
Owner:HENAN AGRICULTURAL UNIVERSITY

Display panel and electronic equipment

The invention relates to the electronic technology field and discloses a display panel and electronic equipment. The display panel comprises a TP IN-Cel liquid crystal display module, a resonance layer and a shielding layer. The resonance layer is located between the TP IN-Cel liquid crystal display module and the shielding layer. The resonance layer is located on the backlight side of the TP IN-Cel liquid crystal display module. In the display time slot, the resonance layer keeps first constant voltage. In the touch control scanning time slot, a touch control sensor and a public electrode both receive scanning signals and are used for charging and discharging electricity of the touch control sensor. The resonance layer is used for receiving pre-set voltage signals. The pre-set voltage signals and the scanning signals are the same in terms of phase and amplitude. The embodiment of the invention further provides the electronic equipment. Compared with the prior art, the embodiment has following beneficial effects: coupling load between a TP plate and the shielding layer can be reduced and power consumption is reduced; energy utilization rate is increased; capacitance change amount sensed by a TP sensor is increased; and detection flexibility is improved.
Owner:SHANGHAI CHUANGGONG COMM TECH

Triazinyl rare earth complex nanomaterial and preparation method and application thereof

The invention discloses a hierarchical porous triazinyl rare earth complex {[CeL(H2O)2].2H2O}n nanomaterial and a preparation method and application thereof. A gel-thermal aging process is adopted to ultrasonically prepare the hierarchical porous metal-organic frametwork complex {[CeL(H2O)2].2H2O}n nanomaterial; the material can modify an electrochemical working electrode to prepare a chiral triazinyl rare earth complex nanomaterial sensor; and a three-electrode system can be adopted to conveniently test the content of (R)-(+)-1-phenethylamine and (S)-(-)-1-phenethylamine enantiomers.
Owner:UNIV OF JINAN

Plasma display apparatus

A plasma display apparatus comprises a waveform generator (WG) coupled between first and the second electrodes (SEl, CEl) to supply, across plasma cells (PCij), a sustain voltage (VCP) with slopes comprising a main part (MA) and a minor part (MI) succeeding the main part (MA). The main part has a duration longer than a formative time 5 lag (FTL) of the plasma cells (PCij), and the minor part has a smaller amplitude than the main part (MA). The plasma cells (pCij) are ignited and sustained by the minor part (MI). The main part (MA) has less steep slopes than the prior-art waveform. Consequently, the EMI produced by the main part (MA) will be at a lower frequency. The minor part (MI) has an amplitude which is relatively low and thus does not add considerably to the EMI, even 10 when its slopes are relatively steep. As the plasma is neither ignited nor sustained by the main part (MA), the main part (MA) further has a lower amplitude than the slope of the prior art and thus produces less EMI.
Owner:KONINKLIJKE PHILIPS ELECTRONICS NV

Human HLA region gene copy number variation detection method

InactiveCN106967801AReasonable and accurate detectionIntuitive and Simplified Analysis ProcessMicrobiological testing/measurementPrenatal diagnosisCel
The invention discloses a human HLA region gene copy number variation detection method. The method includes: firstly, acquiring human tissues, blood or other body fluid genomes as samples, hybridizing the samples with CytoscanHD chips, washing the chips after hybridization is finished, and performing data collection in a data collection system of a GCD3000 system to generate CEL files; secondly, inputting the CEL files into CHAS software to perform primary quality control, importing Cychp files generated by the chips qualified in quality control into the CHAS software, and screening out a target marker according to three indexes including copy number characteristics, marker quantity and CNV gain and CNV loss fragment sizes. By precision of the CytoscanHD chips in detection of copy number variation, a direct and simplified analysis process of the CHAS software and specific setting of CHAS software intervals, accuracy in HLA region gene copy number variation detection is greatly improved, obtained data are higher in reliability and trueness, and prevention of habitual abortion and prenatal diagnosis are guaranteed significantly.
Owner:华子昂

Method for extracting CEL I nuclease in celery

The invention relates to a method for extracting CEL I nuclease in celery and discards measures adopting a plurality of high-speed or ultra-speed centrifugation and a plurality of gel filtration and ion exchange chromatography in the prior art. The method comprises the following steps: adding a small amount of sodium sulfite to extract so as to reduce and clear colored substances in the extract; adopting a thermal denaturation physical method to rapidly clear a great amount of foreign protein with poor temperature toleration; utilizing the characteristic that CEL I is the combination of glycoprotein and activated concanavalin to combine CEL I nuclease and concanavalin so as to remove the foreign protein and further purify the CEL I; and finally, selecting DEAE-Sepharose FF as a suitable medium for the CEL I by once chromatography purification. Accordingly, the extraction method replaces the time-consuming and expensive measures adopting a plurality of high-speed even ultra-speed refrigerated centrifugation, a plurality of gel filtration and ion exchange chromatography, and the like and achieves the aims that the CEL I nuclease is rapidly extracted from the celery and purified.
Owner:GUANGZHOU UNIVERSITY

Hot metal square thermal analysis sample cup special for high-carbon equivalent

InactiveCN109991262AAccurate CELMaterial crystallisationCooling curveHigh carbon
The invention discloses a hot metal square thermal analysis sample cup special for high-carbon equivalent. The hot metal square thermal analysis sample cup comprises a cup body, a thermocouple and a chilling agent, wherein the thermocouple is fixed in the cup body, the chilling agent is fixed on the bottom surface of the inner cavity of the cup body and composed of the following components of by weight, 7-8 parts of tellurium, 0.5-1 part of aluminum, 0.5-1 part of titanium and 1-2 parts of sulfur, and the above components are uniformly mixed after making 0.5-1.5 mm particles to form the chilling agent. The chilling agent in the invention can promote that the molten iron whether it is hypoeutectic or hypereutectic and whether it is subjected to inoculation treatment can be solidified by white mouth, and shows a long eutectic stop platform on the cooling curve, thereby obtaining accurate CEL, C and Si values.
Owner:南京钢铁集团盛达实业有限公司

Fluorescent material and preparation method thereof

The invention provides a fluorescent material having a specific chemical formula of Cel-xAl3(BO3)4:xLn, wherein x is not less than 0.005 and not more than 0.3, and Ln is one or two of Tb and Dy. The invention also provides a method for preparing the fluorescent material, comprising the following steps of: (1) weighing raw materials of corresponding compounds according to a stoichiometric ratio of the composition of the chemical formula; (2) sufficiently mixing the raw materials after the raw materials are ground or ball-milled; (3) carrying out first high temperature sintering on the mixed material; and (4) grinding the material obtained by the first high temperature sintering, carrying out second high temperature sintering, and cooling to obtain the fluorescent material. The preparation method disclosed by the invention comprises two reaction processes of oxidized sintering and reduced sintering, is beneficial to dispersions of rare earth activator ions in a matrix material and is easy for crystals of the fluorescent material to grow up. The fluorescent material prepared by adopting the method has the characteristics of high stability, high excited photoluminescence efficiency and the like.
Owner:OCEANS KING LIGHTING SCI&TECH CO LTD +1

Immortalization chicken embryo hepatocyte line as well as preparation method and application thereof

The invention discloses an immortalization chicken embryo hepatocyte line as well as a preparation method and application thereof. The immortalization chicken embryo hepatocyte line CEL-hMRP18S-2 is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 6, 2016; the preservation number is CGMCC NO.12333; the preservation address is Microbiology Research Institute of China Science Academy, #3, Yard 1, West Beichen Road, Chaoyang District, Beijing. The obtained immortalization chicken embryo hepatocyte retains main characteristics and main functions of the primary generation of chicken embryo hepatocyte; the immortalization chicken embryo hepatocyte can be amplified and cultured without limit in vitro; the hepatocyte line still has high activity after subculturing for 100 generations in vitro, can retain differentiation and proliferation and is free of senescence and apoptosis; when the immortalization chicken embryo hepatocyte line is applied to proliferation of type I aviadenoviruses, the preparation efficiency of virus antigens can be obviously improved.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES

A method for detecting advanced glycation end products in mainstream cigarette smoke

The invention discloses a method for detecting advanced glycation end products (AGEs) in main stream smoke of a cigarette. The method specifically comprises analysis and determination of carboxy methyl lysine (CML) and carboxyethyl lysine (CEL). The method comprises the following steps: trapping total particulate matter of cigarette smoke by virtue of a Cambridge filter; carrying out ultrasonic extraction on the filter with a hydrochloric acid solution; and carrying out analysis by combining a cation exchange solid-phase extraction column purification method with liquid chromatogram-tandem mass spectrometry (LC-MS / MS). The method has the characteristics that (1) a detection method for AGEs in the main stream smoke of the cigarette is built for the first time; (2) a sample pretreatment method is built according to the characteristics of cigarette smoke sample matrix, derivative reaction is not required, and the pretreatment is simple and efficient; and (3) the method is high in sensitivity, good in qualitative accuracy and suitable for analysis of large batches of samples.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC

Evaluation method for the influence of jack-up drilling ship's pile insertion on the pile foundation adjacent to the jacket platform

The invention relates to a method for evaluating influence of self-elevating drilling ship pile pitching on an adjacent jacket platform pile foundation. The method comprises the steps of 1, determining the uniform equivalent elasticity modulus of a soil layer on one side of a jacket pile; 2, building a three dimensional structure analysis model and a three dimensional elastic-plastic finite element CEL (Couple Eulerian-Lagrangian) model and setting an elasticity modulus; 3, determining angles of environmental parameters; 4, determining UC value distribution of a pile body, pile head force and pile head displacement data under the environmental load effect; 5, determining the pile body displacement of the jacket pile foundation under the pile pitching squeezing effect; 6, building a pile soil non-linear footing beam module and calculating pile body internal force; 7, programming under MS EXCEL according to design codes in oceaneering to obtain the UC value distribution of the pile body under the action of the squeezing effect; 8, determining the UC value of the pile body under the coupling of the environmental load effect and the squeezing effect; 9, checking the UC value of the pile body under the coupling condition; 10, analyzing the jacket pile foundation; 11, checking the analysis result and judging whether the design requirement is met or not.
Owner:CHINA NAT OFFSHORE OIL CORP +1

Hsa-miR-625-5pdetection kit based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and detection method thereof

A hsa-miR-625-5pdetection kit based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and a detection method of the hsa-miR-625-5pdetection kit relate to MicroRNA. The detection kit is provided with a kit body, a clapboard, an exogenous reference bottle, a stem-loop reverse transcription reagent bottle, and a real-time fluorescent quantitative PCR reagent bottle. The miRNA in a sample is extracted, and if the sample is a serum / plasma sample or other liquid samples, then 5 mu L exogenous reference cel-miR-39 with the concentration of 5 n mol, provided by the reagent bottle, is added after the sample is fully split, and the mixture is oscillated in vortex; but if the sample is a cell or tissue sample, then exogenous reference cel-miR-39 is not added; a stem-loop reverse transcription reagent provided by the reagent bottle is used to reversely transcribe miRNA into cDNA; a real-time fluorescent quantitative PCR reagent provided by the reagent bottle is used to conduct real-time PCR amplification; various data provided by an analytical instrument are integrated together, and reasonable threshold and reference line are set for result analysis.
Owner:ZHONGSHAN HOSPITAL XIAMEN UNIV

Process For Recycling Solid Supports For Cultivation Of Anchorage-Dependent Cells

This invention relates to methods for use in industrial production of proteins. Specifically, the present invention provides a method of recycling solid supports for cultivation of anchorage-dependent cells such as, e.g., microcarriers and Fibra-Cel® disks. Solid supports recycled by a method of the present invention allow obtaining a protein productivity level comparable to the productivity level obtained with non-recycled solid support. The method comprises the steps of rinsing with water, rinsing with a sodium hydroxide solution and second rinsing step with water.
Owner:ARES TRADING SA
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