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A method for detecting advanced glycation end products in mainstream cigarette smoke

A technology for mainstream smoke and end products of cigarettes, which can be used in measurement devices, instruments, scientific instruments, etc., can solve problems such as cumbersome and time-consuming, and achieve the effects of eliminating false positives, high sensitivity, and simple and efficient pretreatment.

Active Publication Date: 2016-08-17
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the particularity of the matrix of food samples, it is generally necessary to perform degreasing, reduction, protein precipitation, protein hydrolysis and other steps on the sample first. [3] , followed by solid-phase extraction technique [3] or derivatization reaction [4] In order to improve the detection sensitivity, the pre-treatment is very tedious and time-consuming

Method used

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  • A method for detecting advanced glycation end products in mainstream cigarette smoke
  • A method for detecting advanced glycation end products in mainstream cigarette smoke
  • A method for detecting advanced glycation end products in mainstream cigarette smoke

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The balanced and screened cigarettes were smoked according to the standard conditions stipulated in YC / T29-1996, and the total particulate matter of 4 cigarettes was captured by Cambridge filter. Put the filter in a 50 mL conical flask with stopper, add 100 µL of d4-CML and d4-CEL solution, add 30 mL of 0.1M hydrochloric acid solution, and conduct ultrasonic extraction for 30 min. Load 15mL of the extraction solution onto a Dikma ProElut PXC (150mg / 6mL / 30pkg) cation-exchange solid-phase extraction column (activate with 3mL of methanol and 3mL of 0.1M hydrochloric acid before loading), and then rinse with 3mL of 0.1M hydrochloric acid and 3mL of water Wash, and finally elute with 10mL 5% ammonia-methanol solution. During solid phase extraction, the liquid flows down naturally by gravity. The eluent was concentrated using a nitrogen blowing concentrator (the water bath temperature was 35 o C) Blow dry, then add 1mL water to redissolve, add the solution to a chromatograp...

Embodiment 2

[0034] Choose 10 kinds of Kentucky reference cigarettes 3R4F and commercially available cigarette samples, smoke according to the standard conditions stipulated in YC / T 29-1996 respectively, adopt the Cambridge filter disc to suck the total particulate matter of 4 cigarettes, and then according to the description in Example 1 Steps for sample processing analysis. The results of the experiment showed that CML and CEL were not detected in the mainstream smoke of Kentucky reference cigarette 3R4F and 10 commercially available cigarette samples.

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Abstract

The invention discloses a method for detecting advanced glycation end products (AGEs) in main stream smoke of a cigarette. The method specifically comprises analysis and determination of carboxy methyl lysine (CML) and carboxyethyl lysine (CEL). The method comprises the following steps: trapping total particulate matter of cigarette smoke by virtue of a Cambridge filter; carrying out ultrasonic extraction on the filter with a hydrochloric acid solution; and carrying out analysis by combining a cation exchange solid-phase extraction column purification method with liquid chromatogram-tandem mass spectrometry (LC-MS / MS). The method has the characteristics that (1) a detection method for AGEs in the main stream smoke of the cigarette is built for the first time; (2) a sample pretreatment method is built according to the characteristics of cigarette smoke sample matrix, derivative reaction is not required, and the pretreatment is simple and efficient; and (3) the method is high in sensitivity, good in qualitative accuracy and suitable for analysis of large batches of samples.

Description

technical field [0001] The invention belongs to the analysis and detection technology research of harmful substances in cigarette mainstream smoke, and specifically relates to a liquid chromatography-tandem mass spectrometry method for detecting advanced glycation end products (AGEs) (including carboxymethyllysine) in cigarette mainstream smoke (CML) and carboxyethyllysine (CEL)) methods. Background technique [0002] Advanced glycation end products (AGEs) are produced by the reaction of sugar and protein or fat, and mainly exist in foods cooked at high temperatures. When the content of AGEs is high, the human immune system is in an inflamed state, which has harmful effects such as promoting inflammation, cardiovascular disease, diabetes and accelerated aging [1] . The most studied AGEs are carboxymethyllysine (CML) and carboxyethyllysine (CEL). The formation of AGEs is related to the Maillard reaction. It is formed by the reaction of reducing sugars, proteins, and fats. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 余晶晶颜权平王昇赵阁王冰蔡君兰丁丽于永杰谢复炜张晓兵
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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