The invention discloses a method for testing the
toxicity of high
algae-laden
aquatic organisms. The method comprises the steps that
saccharomyces cerevisiae is cultured in a
solid medium containing 5% of YPD and 1.5% of
agar firstly, after clone is grown out, a YPD fluid medium of 5% is inoculated with monoclone, and overnight shake cultivation is conducted based on 180-250 rpm and 25-58 DEG C;
phosphate buffer is added to a culture solution with the OD value of 1.5-1.8 to obtain a blank sample A, a high
algae-laden
water sample is added to the culture solution with the OD value of 1.5-1.8 to obtain a detection sample B, and the two samples are placed on a mixer for oscillation lasting 2 h;
cell washing is conducted on the sample A and the sample B twice by means of PBS, and
cell suspension with the concentration of 1*105-1*106
cel ls / mL is prepared; the sample A and the sample B are both added to
Annexin V-FITC of 5 mug / mL to be evenly mixed, and then
propidium iodide of 5 mug / mL is added to be evenly mixed; reaction is conducted at the
room temperature away from light for 5-15 min;
flow cytometry detection is conducted within 1 h; the proportion of dead cells, the proportion of living cells and the proportion of apoptotic cells are detected by means of
flow cytometry; the
apoptosis rate and the death rate of the sample B are compared with those of the blank sample A, so that water
toxicity is judged. Early warning
technical support is provided for high
algae-laden
water pollution, so that high
water supply quality is guaranteed.