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A kind of immobilized microbial system and its preparation method and detection method of water toxicity

A microbial carrier and microbial technology, which is applied in biochemical equipment and methods, microbial determination/inspection, immobilization on or in inorganic carriers, etc., can solve the problems of poor detection effect and low activity, and achieve good detection effect. The effect of high activity and simple detection method

Active Publication Date: 2019-06-21
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The immobilized biosensor biofilm provided by the prior art has low activity and poor detection effect when used for water toxicity detection

Method used

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  • A kind of immobilized microbial system and its preparation method and detection method of water toxicity
  • A kind of immobilized microbial system and its preparation method and detection method of water toxicity
  • A kind of immobilized microbial system and its preparation method and detection method of water toxicity

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preparation example Construction

[0064] The invention provides a preparation method of a fixed microbial system, comprising:

[0065] Attaching microorganisms to the surface of the microbial carrier to obtain an immobilized microbial system; or

[0066] Cultivate microorganisms in situ on the surface of the microbial carrier to obtain a fixed microbial system;

[0067] The microbial carrier is prepared by carbonizing plant tissue.

[0068] In the present invention, the microorganisms and microbial carriers are consistent with the microorganisms and microbial carriers described in the above technical solution, and will not be repeated here.

[0069] The present invention has no special limitation on the method for attaching microorganisms to the surface of the microbial carrier, and those skilled in the art can transfer the microorganisms to the surface of the microbial carrier according to actual operating conditions.

[0070] In the present invention, the temperature for culturing microorganisms in situ is...

Embodiment 1

[0110] Wash the loofah and cut it to the desired size. Put it into a reaction kettle, carry out carbonization at 180°C for 6 hours, put the obtained carbonized product into ethanol to remove organic residues; then rinse with secondary water and freeze-dry under the condition of 0.08mpar for later use to obtain the microbial carrier.

[0111] The microbial carrier prepared in Example 1 is characterized by SEM, and the detection results are as follows: figure 1 as shown, figure 1 It is the SEM image of the microbial carrier prepared in Example 1 of the present invention.

Embodiment 2

[0113] The microbial carrier was prepared according to the method described in Example 1, the difference from Example 1 was that scallions were used instead of loofah pulp.

[0114] Carry out SEM characterization to the microbial carrier that embodiment 2 prepares, detection result is as follows figure 2 as shown, figure 2 It is the SEM image of the microbial carrier prepared in Example 2 of the present invention.

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Abstract

The invention provides a fixed microbial system, comprising: a microbial carrier prepared by carbonizing plant tissue; and microorganisms attached to the surface of the microbial carrier. The invention also provides a preparation method of the fixed microorganism system and a detection method of water body toxicity. The present invention adopts plant carbonization tissue one-step method to prepare the fixed microbial system, and the preparation method is simple; and the fixed microbial system provided by the present invention adopts the attachment method to fix the microorganisms, so that the microorganisms are naturally deposited on the surface of the pores of the carrier material without cross-linking solidification process; in addition, the microbial carrier used in the present invention has a three-dimensional space network structure and has better mass transfer performance. Therefore, the fixed microbial system provided by the present invention has better activity in the process of detecting water body toxicity, can be repeatedly applied to water body toxicity detection, and has better detection effect.

Description

technical field [0001] The invention relates to the technical field of water body detection, in particular to a fixed microbial system, a preparation method thereof and a water body toxicity detection method. Background technique [0002] Along with the progress of society, human beings are constantly improving their influence on the surrounding environment while improving themselves, and the environmental pollution caused by this reacts on human beings, becoming an obstacle restricting the development of human society. At present, issues such as strengthening environmental protection, preventing and treating polluted environments have become the primary concerns of scientific researchers and environmental experts, and they are also huge challenges that all mankind needs to face together. In the field of environmental protection, environmental monitoring, as an important technology for researching, analyzing, evaluating, and grasping environmental quality conditions, detecti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12Q1/02
CPCC12N11/14C12Q1/025G01N33/1866G01N2520/00
Inventor 翟俊锋刘玲余登斌董绍俊
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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