The invention discloses a method for detecting a miRNA (micro ribonucleic acid) based on a
graphene oxide /
nucleic acid dye detection platform and a
nucleic acid constant-temperature amplification technology. The method comprises the following steps of: first, designing a specific
DNA (deoxyribonucleic acid) hairpin probe and an amplification primer according to the sequence of a target miRNA, mixing the
DNA hairpin probe, the primer, the target miRNA, DMSO (dimethylsulfoxide), an
enzyme with a strand displacement amplification property and necessary reactants to carry out a constant-temperature amplification reaction, and then, adding the
graphene oxide /
nucleic acid dye detection platform to incubate, and carrying out
fluorescence detection. When the target miRNA exists, a detection
system has an obvious fluorescent
signal enhancement phenomenon, and in a condition that the target miRNA does not exist, a fluorescent
signal of the detection
system is quite weak. By using the method, the purpose of highly sensitively, specifically, simply and quickly detecting a target molecule by using a non-modifying probe is realized, and the method is quite suitable to popularize in the application of actual detection.