151 results about "Single species" patented technology

Embodiments of the present invention are directed to methods and devices / systems for amplifying genetic material and may include providing a water-in-oil emulsion in a continuous flow. The emulsion may include a plurality of water droplets comprising microreactors. Each of the plurality of microreactors may include a single bead capable of capturing a nucleic acid template, a single species nucleic acid template and sufficient reagents to amplify the copy number of the nucleic acid template. The method also includes flowing the emulsion across a first temperature zone and a second lower temperature zone to thermally process the microreactors to amplify the nucleic acid template by polymerase chain reaction.

Provided is an array including a solid support having a surface, the surface having a plurality of wells, the wells containing a gel material, the wells being separated from each other by interstitial regions on the surface, the interstitial regions segregating the gel material in each of the wells from the gel material in other wells of the plurality; and a library of target nucleic acids in the gel material, wherein the gel material in each of the wells comprises a single species of the target nucleic acids of the library. Methods for making and using the array are also provided.

Embodiments of the present invention are directed to methods and devices / systems for amplifying genetic material and may include providing a water-in-oil emulsion in a continuous flow. The emulsion may include a plurality of water droplets comprising microreactors. Each of the plurality of microreactors may include a single bead capable of capturing a nucleic acid template, a single species nucleic acid template and sufficient reagents to amplify the copy number of the nucleic acid template. The method also includes flowing the emulsion across a first temperature zone and a second lower temperature zone to thermally process the microreactors to amplify the nucleic acid template by polymerase chain reaction.

The invention discloses a microbe complexing agent with high efficiency and method using thereof for processing municipal wastewater. The microbe complexing agent contains 5-20 shares of nitrifying bacteria, 5-20 shares of denitrifying bacteria, 5-15 shares of bacillus brevis, 5-20 shares of photosynthetic bacteria, 1-20 shares of saccharomycete, each grame culture medium of single species bacteria contains 1.0-3.5 hundred million numbers of bacteria. The invention adopts improved bio-contact oxidation process with a microbe complexing agent with high efficiency as main body, adopting soft assembly complexing biology stuff as biology carrier, increaing anoxia step in a integral SBQ biochemistry treating pool to make hydrolysis and good oxygen oxidize as an integer, nitrifying and denitrifying finished in a pool, removing effectively ammonia, nitrogen and phosphor via aeration regulation and regulation of microbe reaction process. The microbe complexing agent is in characterized in high efficiency and no pollution, the waste water processing method with low cost, less invest, low operation cost, and the method has strong adaptability, less mud product, having better economy and social benefit.

The invention relates to a biotechnology dealing agricultural discard stem with an highly effective stem decomposing composite bacterial, which dissolves the problem of single species of applying microbiological bacterial, weak breaking up, low total degradability rate of cellulose, long developmental cycle. The composite bacterial comprises the following parts: yellow archespore wool bacterium leather bacterial, mushroom bacterial, haci trichoderma, green trichoderma, coninic trichoderma, aspergillus niger, bacillus subtilis, feed bacillus, bacillus megaterium, candida, distillery yeast, food-yeast, brown sphere azotobacter, thermoactinomyces, and so on. The composite bacterial can overall degrade lignin, cellulose, hemicellulose and other organic material in the stem, has a more than 80% degrading rate, a 10-15 days short cycle, keeps a long time for providing a solid bacteria.

A Δ5 fatty acid desaturase gene, a Δ6 fatty acid desaturase gene, and a Δ6 fatty-acid-chain elongase gene are isolated from a single species of Marchantiales. By introducing these genes into higher plants, transformed plants which can produce arachidonic acid and eicosapentaenoic acid (EPA) are obtained.

A database of genetic variations is analyzed to produce a haplotype map of the genome for strains of a single species. A computational method is used to rapidly map complex phenotypes onto the haplotype blocks within the haplotype map. The specific genetic locus regulating three different biologically important phenotypic traits in mice is identified using these systems and methods.

A structure and method for improving the spatial resolution of a scanning probe microscope (SPM) tip, which has been coated with a layer of chemically-synthesized nanoparticles. The nanoparticles are either single-species or heterogeneous, such that the single-species nanoparticles can be either ferromagnetic, paramagnetic, superparamagnetic, antiferromagnetic, ferrimagnetic, magneto-optic, ferroelectric, piezoelectric, superconducting, semiconducting, magnetically-doped semiconducting, insulating, fluorescent, or chemically catalytic. The layer of nanoparticles is at least two nanoparticles thick, or alternatively, is a single layer of nanoparticles thick, or alternatively, is a single layer of nanoparticles thick and covers only the tip apex portion of the tip, or alternatively, only a single nanoparticle is affixed to the tip apex. Alternatively, the layer of nanoparticles is transformed into an electrically-continuous magnetic film by annealing at a high temperature.

Solar cells and other semiconductor devices are fabricated more efficiently and for less cost using an implanted doping fabrication system. A system for implanting a semiconductor substrate includes an ion source (such as a single-species delivery module), an accelerator to generate from the ion source an ion beam having an energy of no more than 150 kV, and a beam director to expose the substrate to the beam. In one embodiment, the ion source is single-species delivery module that includes a single-gas delivery element and a single-ion source. Alternatively, the ion source is a plasma source used to generate a plasma beam. The system is used to fabricate solar cells having lightly doped photo-receptive regions and more highly doped grid lines. This structure reduces the formation of “dead layers” and improves the contact resistance, thereby increasing the efficiency of a solar cell.

A white-light electroluminescent device having an adjustable color temperature substantially on a predetermined range of a Planckian locus within the 1976 Commission Internationale de l'Eclairage (CIE) uniform chromaticity scale diagram. According to one embodiment, a first light-emitting element having a fixed ratio of at least two different species of emitters combined to produce a set of chromaticity coordinates at a predetermined white point substantially on the Planckian locus. A second light-emitting element having at least a single species of emitters produces a set of chromaticity coordinates. The set of chromaticity coordinates are positioned along a projected line extending from the Planckian locus and through the chromaticity coordinates of the first light-emitting layer. A controller adjusts the voltage or current associated with the first and second light-emitting elements to provide white light with a predetermined range of chromaticity coordinates substantially on the Planckian locus.

The invention relates to a method for ecologically restoring vegetation on the bare rock engineering wound surface, which belongs to the technical field of vegetation ecological restoration. A similarly natural mode is used to accelerate the restoration of the vegetation ecological environment and landscape. The method comprises the following steps: firstly carrying out the pretreatment on the bare rock engineering wound surface, and retaining the definite natural unevenness and fissure degree of the actual slope line (4) of the bare rock within the range from the low level control line (2) to the high level control line (3) of a designed slope line (1) under the preconditions of keeping a stable rock slope base and removing dangerous and floating stones; and by adhering to the concept of artificial guidance and natural selection, adjusting measures to the local conditions according to the geological, geomorphic and climatic conditions and different target vegetations, and adopting different processes and slurries with different components to carry out the spray sowing on the rock slope. The method has low cost and high efficiency, facilitates the retention of soil and plant seed sources, and completes at one pass the vegetation restoration which needs years of accumulation and multiple superposition of the natural force. The vegetation ecological restoration is similar to the nature, but is far quicker than the nature. The prosperous period or declining period of a single species mutation can never appear. The method is particularly suitable for the large-area application, achieves the landscape and ecological effects in the near term, the medium term and the long term, and has practical and long term significance.

The present invention relates to proteome chips comprising arrays having a large proportion of all proteins expressed in a single species. The invention also relates to methods for making proteome chips. The invention also relates to methods for using proteome chips to systematically assay all protein interactions in a species in a high-throughput manner. The present invention also relates to methods for making and purifying eukaryotic proteins in a high-density array format. The invention also relates to methods for making protein arrays by attaching double-tagged fusion proteins to a solid support. The invention also relates to a method for identifying whether a signal is positive.

The present invention relates to a method for detecting one or more nascent RNAs in a tissue sample using FISH. In the method, a plurality of probes (8 to 82) may be used to detect a single species of nascent RNA. Further, a plurality of nascent RNA species may be detected simultaneously using between 8 and 82 probes for each nascent RNA. The invention comprises, in addition, methods of preparing a sample for nascent RNA detection by reducing autofluorescence of the tissue sample. These techniques may be synergistically combined to achieve significantly improved results.

A solventless urethane adhesive layer which proves excellent oxygen and moisture barrier properties is formed from the reaction of A) a low molecular weight polyester formed from a single species of linear aliphatic diol and a single species of dicarboxylic acid with B) a single species of diisocyanate.

The invention proposes a non-toxic unit propellant single component engine, the engine is composed of a thrust chamber and a solenoid valve, and the thrust chamber is composed of an injector and a body. The body consists of two layers of catalytic beds and combustion chambers. The catalytic bed uses fine-grained catalysts for the front bed and coarse-grained catalysts for the rear bed. The active component of the catalyst used is metal iridium, and the iridium content is between 5% and 35%. There is a combustion chamber behind the catalytic bed. A coated refractory metal mesh and a coated refractory metal porous plate exist between the front and rear catalytic beds and between the catalytic bed and the combustion chamber. The solenoid valve is opened, and the non-toxic unit propellant is ejected from the injector, and is catalytically decomposed by the catalytic bed, and the decomposition product and the fuel of the propellant are burned in the combustion chamber, and then enter the nozzle to generate thrust. The problem of the combustion temperature and engine structure layout of the non-toxic unit propellant in the single-unit engine propellant is solved, and the expected effect of stable and reliable decomposition and combustion of the non-toxic unit structure propellant is achieved.

The invention discloses red yeast fish fermented by mixed strains based on lactic acid bacteria, which is made by using fresh fish blocks as a base material, and inoculating the base material with I-type mixed basic bacteria of which the colony count is 10CFUs per gram of fish and II-type mixed basic bacteria of which the colony count is 10spores per gram of fish, and subjecting the inoculated base material to main processing procedures of pickling, fermentation, drying and fumigation to obtain the finished product. The invention adopts a technical proposal that initial culture of microorganism lactic streptococci, lactobacillus bulgaricus and saccharomyces cerevisiae of a single species of the I-type mixed basic bacteria of which the colony count is 10CFUs per gram of fish and initial culture of monascus of the II-type mixed basic bacteria of which the colony count is 10spores per gram of fish are mixed and inoculate with the fish block base material, and the mixture is subjected to pickling, fermentation, drying fumigating and the like to give the red yeast fish blocks, and the technical proposal overcomes the defects that natural fermentation is uneasy to ensure the sanitation and safety of the product and has high salt content. The red yeast fish is suitable for producing various fermented fish blocks.

A white-light electroluminescent device having an adjustable color temperature substantially on a predetermined range of a Planckian locus within the 1976 Commission Internationale de l'Eclairage (CIE) uniform chromaticity scale diagram. According to one embodiment, a first light-emitting element having a fixed ratio of at least two different species of emitters combined to produce a set of chromaticity coordinates at a predetermined white point substantially on the Planckian locus. A second light-emitting element having at least a single species of emitters produces a set of chromaticity coordinates. The set of chromaticity coordinates are positioned along a projected line extending from the Planckian locus and through the chromaticity coordinates of the first light-emitting layer. A controller adjusts the voltage or current associated with the first and second light-emitting elements to provide white light with a predetermined range of chromaticity coordinates substantially on the Planckian locus.

An apparatus and method for maximizing the dissolved concentration of ozone in a liquid. The apparatus includes a dissolution tank having a pressure vessel configured to contain a treated fluid and at least one gas in a head space above the treated fluid, an inlet configured to permit passage of an untreated fluid into the head space, and an outlet configured to permit passage of the treated fluid out of the vessel; a gas supply system configured to transport the at least one gas to the head space; a fluid supply system configured to provide the untreated fluid tank; a bleed-off system for removing gas from the head space so as to maximize the dissolved concentration of the ozone gas in the liquid; and a discharge device configured to pass the treated fluid from the tank into the target liquid.

A mass filter for an ion beam system includes at least two stages and reduces chromatic aberration. One embodiment includes two symmetrical mass filter stages, the combination of which reduces or eliminates chromatic aberration, and entrance and exit fringing field errors. Embodiments can also prevent neutral particles from reaching the sample surface and avoid crossovers in the beam path. In one embodiment, the filter can pass a single species of ion from a source that produces multiple species. In other embodiments, the filter can pass a single ion species with a range of energies and focus the multi-energetic ions at the same point on the substrate surface.

A method for raising the resolving power, specificity, and peak capacity of conventional ion mobility spectrometry is disclosed. Ions are separated in a dynamic electric field comprising an oscillatory field wave and opposing static field, or at least two counter propagating waves with different parameters (amplitude, profile, frequency, or speed). As the functional dependencies of mean drift velocity on the ion mobility in a wave and static field or in unequal waves differ, only single species is equilibrated while others drift in either direction and are mobility-separated. An ion mobility spectrum over a limited range is then acquired by measuring ion drift times through a fixed distance inside the gas-filled enclosure. The resolving power in the vicinity of equilibrium mobility substantially exceeds that for known traveling-wave or drift-tube IMS separations, with spectra over wider ranges obtainable by stitching multiple segments. The approach also enables low-cutoff, high-cutoff, and bandpass ion mobility filters.

This invention discloses a kind of vulcanizer especially for ethylene propylene diene monomers, which uniquely has a mass-fraction composition as follows: 5~60% sulphur, 1~50% peroxide, 1~70% thiazole, 3~50% thiuram, 1~50% dithiocarbamate, 1~25% amine and condensate of aldehyde and amine and 1~25% ethanolamine. The interaction of coexisting enhancers and vulcanizers is adequately utilized and the amount of enhancers is reasonably controlled within a range for frost spray inhibition. Therefore, the vulcanization speed of ethylene propylene diene monomers can be promoted by almost 30% and frost spray on product surfaces can be well solved, which significantly improves the physical and mechanical properties of the vulcanizate. This invention is applicable for several tens of ethylene propylene diene monomers and there requires no large amount of experimental comparison for a single specie. Therefore, the label strength of the technologists is lowered and the production efficiency is raised.

The invention relates to a portunus trituberculatus miers ecological bait. The invention is characterized in that the ecological bait consists of the following raw materials by weight percent: 97-98.9% of fundamental nutrition composition, 1-2% of medicine composition and 0.1-1% of other composition. The ecological bait has the advantages of having the balanced nutrition composition, meeting all physiological needs of nutrition in the artificial culture of single species and in the mixed culture of the portunus trituberculatus miers, effectively reducing the cannibalism rate of the portunus trituberculatus miers, improving the growing speed of the portunus trituberculatus miers, reducing the cultivation pollution, improving the quality of the portunus trituberculatus miers, etc.

The invention relates to a method for manufacturing a yellow dye by utilizing 1-amino anthraquinone production waste residues, which is characterized by comprising the following steps of: performing reduction reaction on the 1-amino anthraquinone production waste residues to obtain a reduction product, namely amino anthraquinone mixture, performing condensation reaction on the reduced material amino anthraquinone mixture and a halogenated heterocyclic compound at 90 to 100 DEG C in the presence of sodium bicarbonate in a medium chlorobenzene to prepare a crude yellow dye in form of yellow powder and with colored light approximate to C.I. vat yellow 1, and performing commercial processing to obtain a commercial yellow dye. The crude yellow dye manufactured by the method has performance capable of reaching the level of an ordinary vat dye, can be applied to combination dyeing and the like completely or partially instead of the C.I. vat yellow 1 and also can be used independently as a single species. In the method, a new approach to the utilization of the 1-amino anthraquinone production waste residues is developed, the waste residues can be converted into available commodities with low cost without producing new wastes, and the requirements of environmental protection are met.

The invention provides extracts and preparations based on the species Tulbaghia violacea (Harv.) (wild garlic), which elicits a significant antimicrobial, preferably antifungal activity in vitro and in vivo, even under field and glasshouse conditions. Moreover, these extracts deriving from the soil parts as well as of the aerial parts of the plant elicit a significant bio-stimulatory activity, expressed, above all, by an increased growth metabolism supporting seed growth. Furthermore, combined extracts or preparations from Tulbaghia violacea and species of the genus Agapanthus show a higher antifungal and bio-stimulatory efficacy as compared to the extracts or preparations of the single species, indicating that synergism is participated in the involved biological processes.

The invention aims to provide a method for absorbing microwaves by adopting a dielectric / ferromagnetic composite material, and is used for solving the deficiency of narrow absorbing frequency band of a single-species wave absorbing material. The composite material takes a lanthanum nickelate powder as a dielectric component, and takes carbon-coated iron cobalt as a ferromagnetic component. The composite material has the advantages of small density and wide frequency band width, and has broad application prospects in electromagnetic shielding, photoelectric materials, electrorheological fluids, functional coatings and the like.

The invention provides a classified group buying system and a method. The method includes: a seller client starts and responds to a commodity sales transaction; a buyer client starts and responds to a commodity purchase transaction; and a classified group buying server presets a transaction condition, receives and matches transaction requests, and performs clearing of transaction businesses. According to the system and the method, the purchase requirements with multiple species and small quantities of a plurality of buyers are collected via the Internet, large-quantity purchase of multiple single species is formed, direct transaction with professional sellers is realized, and the transaction cost is reduced via the manner of classified group buying.

The hepatitis B virus (HBV) capsid is made up of a single species of protein called the core antigen (HBcAg) which self-assembles into particles. The particles are highly immunogenic and are able to present heterologous epitopes to the immune system when the epitopes are inserted into a surface-exposed region of the particles called the “e1 loop”. The structural building blocks of the particles are tightly associated dimers of HBcAg in which the adjacent e1 loops are closely juxtaposed. It is proposed that sequences inserted into the e1 loop are conformationally restrained in the assembled particles when presented in monomeric core protein. The invention seeks to solve this problem by covalently linking core proteins as tandem copies (e.g., as dimers) so that insertions can be made independently in each copy. This is particularly useful for insertion of large sequences into the e1 loop because it allows such sequences to be inserted into just one copy of the core protein per tandem repeat, thereby reducing potential conformational clashes in assembly. Alternatively, a different sequence may be inserted into each e1 loop of a tandem repeat, thus increasing the flexibility of HBcAg particles as an epitope delivery system.

The invention relates to a nano selenium tea noni health food and a production method thereof. The nano selenium tea noni health food comprises the following effective constituents according to the parts by weight: 10-42 of organic selenium tea powder, 5-18 of vitamin C, 0.1-0.8 of beta-carotene, 10-30 of noni fruit powder, 0.1-0.8 of sweetening agent, 0.5-1.5 of vitamin A and 1-10 of vitamin E. The production processes of the nano selenium tea noni health food comprise preprocessing, pulverization, granule screening, granule mixture, nano grinding, capsule filling and sterilization. The invention can strengthen the antivirus and antitumor capability and the biological activity of a single species and achieves the aims of strengthening immunity and improving the antivirus and antitumor capability of a human body. The health food has no poison and side effects during long-term taking, is suitable for various people to strengthen the immunity, improves the antivirus and antitumor capability of the human body and has the weight-loss effects and the functions of beautifying and maintaining female skin.