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Detection kit for chicken CEL gene promoter 99bp indel polymorphic marker and application of detection kit

A technology of polymorphic markers and promoters, applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., to achieve the effects of accelerating the progress of genetic selection, high resolution, and strong operability

Inactive Publication Date: 2019-08-23
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the CEL gene may be closely related to the growth and slaughter traits of poultry, but the mutation of this gene and its impact on poultry growth and slaughter traits have not been reported yet.

Method used

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  • Detection kit for chicken CEL gene promoter 99bp indel polymorphic marker and application of detection kit
  • Detection kit for chicken CEL gene promoter 99bp indel polymorphic marker and application of detection kit
  • Detection kit for chicken CEL gene promoter 99bp indel polymorphic marker and application of detection kit

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Experimental program
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Effect test

Embodiment 1

[0033] In the present embodiment, the chicken CEL gene promoter 99bp indel polymorphic marker has a nucleotide sequence as shown in SEQ ID NO.1, and the 183-281st position from the 5' end is inserted or deleted.

[0034] The 99bp deletion in the polymorphic marker occurred between the 4309 and 4310 sites upstream of the CEL gene, and the NCBI GenBank accession number of the gene sequence was NC_006104.

Embodiment 2

[0036] The detection primers of chicken CEL gene promoter 99bp indel polymorphic marker in the present embodiment are as follows:

[0037] Upstream primer P-F: 5'-GTCACTCAGAGGCTCCCTTT-3';

[0038] Downstream primer P-R: 5'-TGGCTAGGAACCCACGATAC-3'.

Embodiment 3

[0040] The detection kit for the chicken CEL gene promoter 99bp indel polymorphism marker in this example includes the primers shown in Example 2, and also includes dNTPs, PCR reaction buffer, and DNA polymerase.

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Abstract

The invention relates to a detection kit for a chicken CEL gene promoter 99bp indel polymorphic marker and application of the detection kit, and belongs to the technical field of molecular genetics breeding. According to the detection kit and the application, the CEL gene promoter 99bp indel polymorphic marker is found and can be used for assistant selection and molecular breeding of chickens; specifically, according to a sequence as shown in SEQ ID NO.1, a primer found to be without 183rd to 281st sites from the 5' end of the sequence is designed and amplified, whether the 183rd to 281st sites are lost or not is judged according to the size of the amplification product, then whether a detection sample is DD or II or ID gene type is judged, and the slaughter performance of local chickens is improved by enhancing breeding of DD gene type individuals. The application method established through the detection kit does not need digestion, is high in resolution, accurate in type judgment, easy to operate, low in cost and short in period, does not need special instruments, and is easy to popularize.

Description

technical field [0001] The invention relates to a chicken CEL gene promoter 99bp indel polymorphism marker detection kit and application thereof, belonging to the technical field of biological breeding. Background technique [0002] Growth performance and carcass traits are the main economic traits of livestock and poultry. How to improve the economic traits of local livestock and poultry breeds without changing the germplasm characteristics and maintain the sustainable development of local livestock and poultry is particularly important with the help of modern molecular markers . [0003] Carboxyl ester lipase (CEL) is a kind of lipase secreted by the pancreatic acinus. They are inactive zymogens, but after being secreted into the duodenum, they are activated by bile salts and participate in the hydrolysis of various lipid esters (cholesteryl esters) , triglycerides, phospholipids, fat-soluble vitamins, etc.). Studies have shown that after mutations in the variable number...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 韩瑞丽王香南李转见康相涛郭亚苹李东华闫峰宾蒋瑞瑞李红田亚东李国喜孙桂荣刘小军刘家欣
Owner HENAN AGRICULTURAL UNIVERSITY
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