The invention discloser SSR markers lined with major genes of cotton
fiber strength, which is obtained by the following steps: generating F2 and F2:3 populations by using a cotton search 41 line sGK9708 selected from cultivated varieties of
gossypium hirsutum and a high quality line 0-153 of
gossypium hirsutum as parents; allowing generation within the family of the F2:3
population to self cross till the F2:6 generation, performing within-family individual selection of the F2:6 generation once, and planting
two generation till F6:8; performing polymorphism screening of the parents by using SSR primers and creating an RIL
population linkage map; and performing the multi-environment major QTL screening of the cotton
fiber strength to screen 6 QTLs of a cotton
fiber strength character from line 0-153, wherein 5 QTLs are multi-environment stable QTLs and are FS1 linkage marker NAU2119330, FS2 linkage markers BNL2572125, BNL1064110 and DPL0874210, FS4 linkage markers are NAU1048250 and NAU2627350, and FS5 linkage markers BNL1421200 and NAU2730450. The SSR markers lined with the major genes of the cotton
fiber strength are screen from high fiber quality materials and used as molecular markers to perform early auxiliary selection on
a DNA level to improve the selection efficiency of the cotton
fiber strength.