38 results about "Neural tube" patented technology

The invention belongs to the field of biotechnology and medicine and relates to an application of miR-219, a new marker of human brain glioma. The fact that the expressions of miR-219 in the human brain glioma tissues and cells are obviously reduced is confirmed for the first time, and the facts that miR-219 can directly control Hh-Gli2 and Pdgfra signal paths during early development of the neural tube of embryo and occurrence of the brain glioma and can obviously suppress proliferation and infiltration of human glioma cells are confirmed. Further quantitative analysis of clinical brain glioma specimens at different levels shows that miR-219 can serve as the marker for detecting the brain glioma.

The invention discloses a preparation technology of a novel nerve conduit, and belongs to the technical field of tents of biological tissue engineering. The preparation technology sequentially comprises a preparation technology of GDNF (glial-derived neurotrophic factor)-carried gelatin microspheres, a synthesizing technology of gelatin-methacrylamide hydrogel, a preparation method of polycaprolactone/gelatin electricity texture nerve conduits, and a combining method of the electricity texture nerve conduits and the GDNF-carried gelatin microspheres. The preparation technology has the advantages that a tent structure with good degradability, biological compatibility and biological safety is realized, and the GDNF is controlled and released by the GDNF-carried gelatin microspheres; compared with blank groups and collagen conduit groups, the regeneration of peripheral nerves is effectively promoted, and the structure and function of restoring damaged nerves can reach the effect similar to the effect of autotransplantation groups; the defect nerves can be bridged, and the stability of axon regeneration environment can be maintained; the invasion of peripheral connective tissues is prevented; the saturing of near and far-heart breaking ends of smaller nerve defect is solved, and the restoration of larger nerve defect is solved.

An oral cavity CBCT image mandibular neural tube automatic recognition method based on Mask RCNN comprises the following steps: collecting oral cavity CBCT image data, preprocessing a coronal diagramin the oral cavity CBCT image data, removing an image which does not display a mandibular neural hole in the coronal diagram, performing format compression on the image, and manually aiming at the mandibular neural hole; generating a sighting frame, positioning the rectangular frame, obtaining a binary mask mask of the target instance, and establishing a neural network model; training a model; andrecognizing and displaying the mandibular nerve hole through the trained model. According to the method, the Mask RCNN is used for automatically identifying the mandibular neural tube in the CBCT image. Machine recognition is used for replacing manual recognition of coronal diagram tooth nerve holes, so that the labor cost is saved, the stability of the generated mandibular nerve tube track is improved, and the method has good recognition speed and precision.

The present invention relates to a pregestational gene alarm diagnosis kit and its detection method, including DNA extraction liquor for extracting DNA template of testee, PCR mixed liquor for amplifying specific fragment on the genomic DNA of testee, DNA polymerase, PCR product enzyme excision mixed liquor, restriction endonuclease, positive control template of homozygous mutation and coupling pipe for making PCR reaction. The described alarm gene includes N5, N10-methylene tetrahydrofolate reductase and methionine synthetase reductase gene, their mulant sites are respectively 667-cytosine-thymine and 66 adenine-guanine, and their primer sequences respectively are 5'-TGA AGG AGA AGG TGT CTG CGG GA-3', 5'-AGG ACG GTG CGG TGA GAG TG-3', 5'-GCA AAG GCC ATC GCA GAA GAC AT-3' and 5'-GTG AAG ATC TGC AGA AAA TCC ATG TA-3'. It can be used for pregestational diagnosis of that filial generation produces neural tube deform or not.

A self-renewing restricted stem cell population has been identified in developing (embryonic day 13.5) spinal cords that can differentiate into multiple neuronal phenotypes, but cannot differentiate into glial phenotypes. This neuronal-restricted precursor (NRP) expresses highly polysialated or embryonic neural cell adhesion molecule (E-NCAM) and is morphologically distinct from neuroepithelial stem cells (NEP cells) and spinal glial progenitors derived from embryonic day 10.5 spinal cord. NRP cells self renew over multiple passages in the presence of fibroblast growth factor (FGF) and neurotrophin 3 (NT-3) and express a characteristic subset of neuronal epitopes. When cultured in the presence of RA and the absence of FGF, NRP cells differentiate into GABAergic, glutaminergic, and cholinergic immunoreactive neurons. NRP cells can also be generated from multipotent NEP cells cultured from embryonic day 10.5 neural tubes. Clonal analysis shows that E-NCAM immunoreactive NRP cells arise from an NEP progenitor cell that generates other restricted CNS precursors. The NEP-derived E-NCAM immunoreactive cells undergo self renewal in defined medium and differentiate into multiple neuronal phenotypes in mass and clonal culture. Thus, a direct lineal relationship exists between multipotential NEP cells and more restricted neuronal precursor cells present in vivo at embryonic day 13.5 in the spinal cord. Methods for treating neurological diseases are also disclosed.

Provided are genetically engineered cells comprising a neural stem cell and retroviral expression system in the neural stem cell, which is capable of expressing homeodomain transcription factor Nkx6.1 protein but does not express homeodomain transcription factor Irx3 protein or homeodomain transcription factor Nkx2.2 protein; which is capable of expressing homeodomain transcription factor Nkx6.1 protein and homeodomain transcription factor Irx3 protein; and which is capable of expressing homeodomain transcription factor Nkx2.2 protein or homeodomain transcription factor Nkx2.9 protein. Also provided are methods of generating such genetically engineered motor neurons, V2 neurons, and V3 neurons. Also provided are methods of treating subjects having a motor neuron injury or a motor neuron disease comprising implanting in injured/diseased neural tissue of the subject any of the provided genetically engineered cells, administering to such neural tissue retroviral expression systems which are capable of expressing the appropriate homeodomain protein(s), or transfecting neural stem cells with a retroviral vector, which is capable of expressing the required homeodomain transcription factor protein(s). Provided is a method of determining whether a chemical compound affects the generation of a motor neuron from a neural stem cell.

The invention discloses a temporal bone model for surgery training and a forming method thereof, which belong to the technical field of ear surgery training aids. The temporal bone model for surgery training comprises a cortical bone, a cancellous bone, a semicircular canal, an ear bone and a face neural tube, wherein the cancellous bone is of a porous structure; the semicircular canal and the face neural tube are of cavity structures; the ear bone is of a solid structure; the semicircular canal, the ear bone and the face neural tube are integrally formed by adopting aluminum oxide ceramics through 3D (Three-dimensional) printing; the cancellous bone is prepared from three raw materials: mixed powder, water and graphite powder; the mixed powder is prepared from mixing 85 percent by weightof clay and 15 percent by weight of feldspar powder; the cortical bone is prepared from two raw materials: mixed powder and water. The invention provides the temporal bone model for surgery training and the forming method thereof. The temporal bone model for surgery training is higher in biofidelity, lower in manufacturing cost, and capable of greatly improving a surgery training effect, shortening a doctor training period, reducing doctor training cost, and benefitting patients.

The invention discloses a multifunctional oral implant practice model, which comprises a maxillary model and a mandible model. The maxillary model comprises maxillary soft tissue, a maxillary bone, maxillary teeth and a maxillary connection member. The maxillary connection member is fixedly arranged in the middle of the top surface of the maxillary bone; the maxillary soft tissue wraps the maxillary bone part; and the maxillary teeth are fixedly inserted into corresponding maxillary alveolar sockets. The mandible model comprises mandible soft tissue, a mandible bone, mandible teeth, a mandible tongue base, a mandible neural tube and a mandible connection member. The mandible connection member is fixedly arranged in the middle of the bottom surface of the mandible tongue base; the mandible bone circularly wraps the front side and left and right sides of the mandible tongue base, and wraps the whole mandible neural tube; the mandible soft tissue wraps the mandible tongue base and the part, except the bottom surface, of the mandible bone; and the mandible teeth are fixedly inserted into corresponding mandible alveolar sockets. Training contents are arranged in one set of maxillary and mandible oral cavity model as many as possible, thereby achieving the effects of simulation of clinical simulation, operation convenience of a short-term training class and people availability of production cost.

The invention belongs to the technical field of biological medicine, and particularly relates to a molecular marker for antenatal noninvasive diagnosis of a fetal with neural tube malformation, congenital heart disease or cleft lip and palate and application of the molecular marker. The molecular marker is composed of one or more selected from CORO1A, DNM2 and ACTR2 proteins for antenatal noninvasive diagnosis of fetuses with neural tube malformation, congenital heart disease and cleft lip and palate. The molecular marker for antenatal noninvasive diagnosis is applied to preparation of products for antenatal screening, early warning, clinical diagnosis and biochemical inspection of fetuses with neural tube malformation, congenital heart disease and cleft lip and palate. According to the invention, the close correlation between the abnormal expression of the proteins (including CORO1A, DNM2 and ACTR2) in the blood of the pregnant woman and the occurrence of the fetal with the neural tube deformity, the congenital heart disease and the cleft lip and palate is found and confirmed for the first time, the quantity of verified samples is large, the result is accurate, and a new way is provided for the prenatal screening, early warning and diagnosis of the fetal with the neural tube deformity, the congenital heart disease and the cleft lip and palate.

The invention specifically discloses a neural crest stem cell preparation and application thereof. The neural crest stem cell preparation provided by the invention is obtained by culturing a neural tube in vitro, wherein the neural tube is derived from a 10-day pregnant mouse embryo. The neural crest stem cell preparation disclosed by the invention and obtained by culturing the neural tube derivedfrom a 10-day pregnant mouse embryo in vitro identifies neural crest stem cells by immunofluorescence; P0 generation cells and P3 generation cells respectively express characteristics of neural crestand do not express the characteristics of neural stem, which indicates that the cultured cells successfully maintain the characteristics of mouse neural crest stem cells in vitro; and in-vitro culture experiments show that the neural crest stem cells can grow stably in a provided culture medium and normal proliferation of cells is maintained.

The embodiment of the invention discloses an automatic marking method and device for a mandibular neural tube and electronic equipment. The method and device are used for marking mandibular neural tube data with high precision. The method comprises the following steps: acquiring first three-dimensional panorama data of a periodontal curved body; expanding the first three-dimensional panorama data into cuboid second three-dimensional panorama data; marking the second three-dimensional panorama data by using a trained 3D neural network model to obtain a mandibular neural tube; wherein the 3D neural network model is obtained by training based on three-dimensional panorama data of a cuboid marked with a mandibular neural tube; and mapping the marked mandibular neural tube to the first three-dimensional panorama data.

The invention provides a training method for simulating tooth implantation. The training method comprises the following steps: S1, acquiring original implant data representing different tooth positions and different bone conditions; S2, acquiring CBCT (Cone Beam Computerized Tomography) data corresponding the original implant data, performing modeling according to the CBCT data, and generating STL (Standard Template Library) data of alveolar bones and teeth; S3, importing neural tube or maxillary sinus or nasal base structure data into the STL data of alveolar bones and teeth; S4, printing a physical model of alveolar bones and teeth according to the STL data imported with the neural tube data in step S3; and S5, performing simulated tooth implantation training using the printed physical model of alveolar bones and teeth. The training method for simulating tooth implantation based on real patient cases is morphologically closer to real patients relative to a high molecular simulation model and animal jaws, so that trainees can be trained on models of real cases.