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Pregestational gene warning diagnostic kit

A kit and gene technology, applied in the pre-pregnancy gene alarm diagnostic kit and its detection field, can solve the problems of side effects of embryo growth and development, failure to reach early warning/diagnosis of neural tube defects, etc., and achieve mature methods, stable and reliable results, and easy operation easy effect

Inactive Publication Date: 2004-06-30
SICHUAN UCAN BIO TECH
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Problems solved by technology

Therefore, the purpose of pre-pregnancy early warning / diagnosis of neural tube defects cannot be achieved; moreover, the above methods have certain side effects on embryo growth and development, so it is necessary to seek an earlier, safer and more sensitive detection method

Method used

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  • Pregestational gene warning diagnostic kit
  • Pregestational gene warning diagnostic kit
  • Pregestational gene warning diagnostic kit

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Embodiment Construction

[0046] The following are embodiments of the present invention:

[0047] The DNA extraction solution in accompanying drawing 1 is made up of erythrocyte lysate and polymerase chain reaction (PCR) buffer / nonionic detergent / proteinase K. Among them, the erythrocyte lysate is sterilized triple distilled water, and the polymerase chain reaction (PCR) buffer / nonionic detergent / proteinase K is composed of trishydroxymethylaminomethane (Tris), magnesium chloride (MgCl 2 ), TritonX-100 (TritonX-100), Tween-20 (Tween-20), wetting agent p-40 (NP-40) and proteinase K (proteinase K); other ingredients are polymerized Enzyme Chain Reaction (PCR) Mixture, Digestion Mixture, DNA Polymerase (Taq Enzyme) Solution and Restriction Endonuclease Solution. Wherein, the polymerase chain reaction (PCR) mixture includes N 5 , N 10 -Methylenetetrahydrofolate reductase polymerase chain reaction (PCR) mixture, methionine synthase reductase polymerase chain reaction (PCR) mixture, respectively by deioni...

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Abstract

The present invention relates to a pregestational gene alarm diagnosis kit and its detection method, including DNA extraction liquor for extracting DNA template of testee, PCR mixed liquor for amplifying specific fragment on the genomic DNA of testee, DNA polymerase, PCR product enzyme excision mixed liquor, restriction endonuclease, positive control template of homozygous mutation and coupling pipe for making PCR reaction. The described alarm gene includes N5, N10-methylene tetrahydrofolate reductase and methionine synthetase reductase gene, their mulant sites are respectively 667-cytosine-thymine and 66 adenine-guanine, and their primer sequences respectively are 5'-TGA AGG AGA AGG TGT CTG CGG GA-3', 5'-AGG ACG GTG CGG TGA GAG TG-3', 5'-GCA AAG GCC ATC GCA GAA GAC AT-3' and 5'-GTG AAG ATC TGC AGA AAA TCC ATG TA-3'. It can be used for pregestational diagnosis of that filial generation produces neural tube deform or not.

Description

technical field [0001] The invention relates to a gene alarm kit and a detection method for predicting / diagnosing whether neural tube defects (neurae tube defects, NTDs) occur in offspring by detecting multiple gene mutation sites of both parents before pregnancy. Background technique [0002] Neural tube defects (NTDs) are a group of severe birth defects mainly including anencephaly and spina bifida, and are the birth defects with the most frequent incidence and the most serious clinical consequences in babies born in my country and the world. one. The incidence rate of neural tube defects in my country is 3.0‰, and the incidence rate of neural tube defects in some northern regions is as high as 10-20‰, which is currently known as the region with the highest incidence of neural tube defects in the world. At least 100,000 children with neural tube defects are born in my country every year (accounting for 1 / 4 to 1 / 3 of the total number of children with neural tube defects in ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/6851C12Q1/6883
CPCC12Q1/6883C12Q1/6851
Inventor 李勇李竹赵如冰杨阳
Owner SICHUAN UCAN BIO TECH
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