Preparation technology of novel nerve conduit
A preparation process and nerve conduit technology, which is applied in the field of biological nerve tissue engineering, can solve the problems of limited donor nerve materials and limited wound pain, and achieve good degradability, restoration of structure and function, and low cytotoxicity
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Embodiment 1
[0047] A preparation process of a new type of nerve guide, which sequentially includes the preparation process of GDNF-loaded gelatin microspheres, the synthesis process of gelatin-methacrylamide hydrogel, the preparation method of polycaprolactone / gelatin electrospun nerve tube and the electrospun Binding method of spun neural tubes and GDNF-loaded gelatin microspheres.
[0048] The preparation process of the preparation of the GDNF-loaded gelatin microspheres specifically includes the following steps:
[0049] A1. Prepare a type B gelatin aqueous solution with a mass volume fraction of 10%, and preheat it to 45°C for later use;
[0050] A2. Put the liquid paraffin containing 1% span80 in a water bath at 45°C for 20 minutes, slowly drop in the above 10ml gelatin aqueous solution, and stir magnetically for 15 minutes in a water bath at 45°C to form an emulsion;
[0051] A3. Put the emulsion obtained in step A2 through an ice bath to quickly cool down the emulsion to 4°C, cont...
Embodiment 2
[0076] The difference with Example 1 is that the 10 μl concentration described in step A7 is 8 μg / ml, and the pH value of the GDNF solution is 7.0; Ammonium persulfate and tetramethylethylenediamine in the same volume were sequentially added to the methacrylamide hydrogel solution at a concentration of 600 mM.
Embodiment 3
[0078] The difference with Example 1 is that the 10 μl concentration described in step A7 is 8 μg / ml, and the pH value of the GDNF solution is 7.5; Ammonium persulfate and tetramethylethylenediamine in the same volume were sequentially added to the methacrylamide hydrogel solution, both at a concentration of 1100 mM.
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