The invention discloses a carrageenan and gelatin microsphere embolization agent and a preparation method thereof. The carrageenan and gelatin microsphere embolization agent comprises mixed glue formed by carrageenan and gelatin, a biodegradable polymer, an emulsifier and a crosslinking agent. The carrageenan and gelatin microsphere embolization agent is a novel microsphere integrated with embolism and medicine administration functions, and can be applied to treatment of diseases such as tumors and the like. By adopting the preparation method of the microsphere, the novel microsphere, which has the advantages of wide grain diameter distribution range, large drug loading capacity, long embolization time, controllable in-vivo degradation time and the like can be prepared. The function of the existing microsphere is perfected, the carrageenan and gelatin microsphere embolization agent is prepared by using an emulsion crosslinking method, the carrageenan and gelatin microsphere embolization agent is simple in process, and convenient to operate, and good news is brought about for a patient.