Naringin microsphere silk fibroin/hydroxyapatite composite scaffold and preparation method thereof

Abstract

The invention discloses a composite scaffold. The composite scaffold is prepared from naringin, gelatin microspheres, hydroxyapatite and silk fibroin. The composite scaffold is prepared by the following steps: 1) dropwise adding a 0.5-1g / ml naringin solution into the gelatin microspheres, then standing at the temperature of 2 to 8 DEG C over night, and performing freeze drying to obtain a naringin / gelatin microsphere compound; 2) mixing the hydroxyapatite and the naringin / gelatin microsphere compound with a 3.5 to 10 percent by weight SF solution, injecting the mixed solution into a pore plate, then performing freeze drying on the sample to obtain an NG / GMs / HA / SF composite scaffold. The traditional SF / HAp composite scaffold is loaded with the traditional Chinese medicinal monomer, and encapsulated with gelatin microspheres conforming to a bone microenvironment, thereby promoting healing of OVF and enhancing the mechanical strength of the fractured vertebral body; the composite scaffoldhas high rheological property, plasticity, biomechanical strength and higher bone formation prompting performance, so that the composite scaffold can be applied to bone defect areas of different shapes, has a durable effect and higher bone tissue repair ability, and is convenient for large-scale popularization and application.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a naringin microglobe silk fibroin / hydroxyapatite composite scaffold and a preparation method thereof. Background technique [0002] Biomaterial scaffolds, as one of the three major elements of regenerative medicine, play an important role in the bone remodeling process, so they are often used in research to treat OVF. Among many biological materials, hydroxyapatite has attracted much attention in the research of bone defect regeneration due to its good osteoconductive properties and similar mineralization process and structure to bone tissue. Silk fibroin is a kind of natural biopolymer with high mechanical strength, controllable biodegradability, good biocompatibility and easy handling. Using modern science and technology to make silk fibroin and hydroxyapatite into a composite scaffold can not only integrate the advantages of the two, but also avoid the shortcoming...

AI Technical Summary

Problems solved by technology

[0003] Polymethyl methacrylate is a commonly used implant material. Although it can provide sufficient support strength and cementation, it also has some obvious disadvantages: it cannot be degraded and absorbed in the body, has poor biocompatibility, has no biological activity, and has no biological activity when polymerized. A large amount of heat is released, and unpolymerized monomers are toxic, etc., resulting in some serious complications that often occur in clinical applications

Studies have found that SF can stimulate the high expression of osteogenic differentiation factors and promote the early and late osteogenic differentiation of osteoblasts, but in vivo experiments only found that it provides an environment for host cell proliferation, and no obvious bone formation was found

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