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41results about How to "Good acid and alkali tolerance" patented technology
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Water-soluble and degradable cross-linking agent, preparation method of gamma-polyglutamic acid water absorbing material using cross-linking agent
The invention discloses a preparation method of gamma-polyglutamic acid water absorbing material. Gamma-polyglutamic acid is used as a main raw material, chitosan oligosaccharide-graft-acrylic acid is used as a biodegradable cross-linking agent, and the gamma-polyglutamic acid hydrogel water absorbing material is obtained after preparation in an aqueous solution. The biodegradable cross-linking agent replaces a common chemical cross-linking agent for preparing the hydrogel, the reaction medium is free of toxic organic solvents, gamma-polyglutamic acid which is safe and nontoxic is used as the main raw material, the water-soluble and degradable chitosan oligosaccharide-graft-acrylic acid is used as a cross-linking agent, and the gamma-polyglutamic acid water absorbing material is prepared in the aqueous solution. The raw materials and mediums do not have harmful substances for human body, the safe, nontoxic and biodegradable hydrogel is obtained, and the hydrogel can be applied to disposable hygiene products, drug carriers, medical articles, etc.
Owner:新疆康润洁环保科技股份有限公司
Application of esterase gene est816 and recombinant esterase thereof in degrading pyrethroid pesticides
InactiveCN107058362AEfficient soluble expressionRetain relative enzyme activityFungiBacteriaCypermethrinHeterologous
The invention discloses an application of an esterase gene est816 and a recombinant esterase thereof in degrading pyrethroid pesticides. Specifically, the invention relates to heterologous expression of esterase gene est816 and a recombinant esterase thereof in degrading pyrethroid pesticides. A recombinant esterase Est816 is constructed via both an Escherichia coli expression system and a Pichia pastoris expression system, has efficient soluble expression in two expression systems, and has good thermal stability and acid and alkali resistance. The recombinant esterase est816 has a strong degradation effect on pyrethroid pesticides (such as cyhalothrin, cypermethrin, fenvalerate, deltamethrin, and so on), and the degradation rate is up to 90% or higher. Therefore, the esterase gene est816 and the recombinant esterase Est816 have broad application prospects in removing residual pyrethroid pesticides.
Owner:SUN YAT SEN UNIV
Salmonella broad-spectrum virulent bacteriophage as well as preparation method and application thereof
ActiveCN112662636AHigh potencyHas cracking abilityAntibacterial agentsDigestive systemSalmonella paratyphi ASerotype
The invention provides a salmonella broad-spectrum virulent bacteriophage. The preservation number of the bacteriophage is CCTCC NO: M 2020839. The invention also provides a preparation process and application of the salmonella broad-spectrum virulent bacteriophage, and the preparation process comprises fermentation and extraction; in the step of fermentation, a salmonella gallinarum attenuated vaccine strain is used as host bacteria for bacteriophage fermentation. The bacteriophage XPARCPS02 disclosed by the invention can be used for cracking salmonella of various serotypes and also has different degrees of cracking effects on drug-resistant bacteria; the salmonella typhimurium lysate lysate has a cracking capability on salmonella typhimurium, salmonella typhimurium drug-resistant bacteria, salmonella gallinarum, salmonella enteritidis, salmonella enteritidis drug-resistant bacteria, salmonella typhimurium, salmonella pullorum, salmonella paratyphi A, salmonella paratyphi B and salmonella dubicularis.
Owner:山东仙普爱瑞科技股份有限公司
Method for analyzing etimicin
ActiveCN101614712AGood acid and alkali toleranceGood durabilityComponent separationChromatographic columnChemistry
The invention relates to a method for detecting etimicin and related substances thereof. The method adopts high performance liquid chromatography; chromatographic conditions are that: polystyrene-divinylbenzene polymer chromatographic column is MKF-RP-ETI (the particle size is between 4 and 10mu m, the column specification is (100-300)*(8.0-4.6)mm (I.D.)); the volume ratio of compositions of a mobile phase is that the volume ratio of an acetate buffer solution (the pH value is between 4 and 7) with a concentration of between 0.10 and 0.50mol / l to acetonitrile is 90-100: 10-0; the flow rate is between 0.8 and 1.2ml / min; a detector is a refractive index detector; and the injection volume is between 10 and 20mu l. The method can finish the on-line detection on the etimicin and the related substances by using the prior HPLC instrument and refractive index detector; and the method is simple, quick and accurate.
Owner:HAINAN AIKE PHARMA
Method for detecting acetylcysteine and related substances thereof
InactiveCN101699281AGood acid and alkali toleranceGood practical valueComponent separationUltraviolet detectorsPhosphate
The invention relates to a method for detecting acetylcysteine and related substances thereof. The method adopts a high performance liquid chromatography, wherein the chromatographic condition is that styrene-divinylbenzene copolymer type superpolymer chromatographic column, namely MKF-RP-MH is 4 to 10 mu meters and (100-300)*(8.0-4.0) millimeters (I.D.); the volume ratio of phosphate buffer (pH is between 2 and 5) with mobile phase compositions of 0.01-0.05 mol / L to acetonitrile is 80-95:20-5; the flowing speed is between 0.8 and 1.2 mL / min; the detecting wavelength detected by an ultraviolet detector is 210nm; and the sample size is between 10 and 20 mu liters. The method does not use ionpairreagent, overcomes the defects that ODS column detecting method does not have acid resistance or low organic phase resistance when detecting the acetylcysteine.
Owner:NANJING UNIV OF TECH
Lactic acid-producing caproiciproducens derived from pit mud for white spirit brewing and application of lactic acid-producing caproiciproducens
ActiveCN112458031AGood acid and alkali toleranceEasy to trainBacteriaMicroorganism based processesBiotechnologyMicrobiology
The invention discloses lactic acid-producing caproiciproducens derived from pit mud for white spirit brewing and application of the lactic acid-producing caproiciproducens. The lactic acid-producingcaproiciproducens is named as Caroiciproducens sp. JNLZ26, and has a deposit number of CGMCC No. 17440. The strain can utilize starch and glucose to ferment and produce caproic acid and lactic acid atthe same time, has a yield of 1.32 g / L for fermenting caproic acid by using starch, and a yield of 10.68 g / L for lactic acid. The strain can be applied to the field of wine brewing, including pit mudpreparation and rice wine flavor improvement, can also be applied to synchronous fermentation production of caproic acid and lactic acid, and has great industrial application value.
Owner:JIANGNAN UNIV +1
Polysulfate flat ultrafiltration membrane and preparation method thereof
The invention discloses a polysulfate flat ultrafiltration membrane and a preparation method thereof, and belongs to the technical field of membrane separation. The preparation method comprises the following steps: fully dissolving or dispersing an additive and a filler in a solvent, dissolving polysulfate in the obtained solution, completely defoaming, coating a supporting plate with the solutionin a scraping manner, and finally immersing the supporting plate in a coagulating bath to form a film. A series of polysulfate ultrafiltration membranes with different pore sizes are prepared by introducing the additive and the filler and changing the concentration of the solvent or a polymer. The addition of the filler can significantly improve the antibacterial and anti-pollution properties, increase the mechanical strength and improve the structural stability, and meanwhile, the polysulfate membrane has excellent acid and alkali resistance and shows wide industrial application potential.
Owner:BEIJING UNIV OF TECH
Separation and application of high-lysis-rate salmonella phage RDP-SA-17118
ActiveCN111100844AHas a strong cracking effectNo significant change in titerAntibacterial agentsViral/bacteriophage medical ingredientsDiseaseSalmonella Phage
The invention discloses separation and application of a high-lysis-rate salmonella phage RDP-SA-17118. The host of the salmonella phage RDP-SA-17118 is salmonella S6, and the phage can form a plaque with a diameter of 2mm-4mm on double plates. Through observation by an electron microscope, the phage has a polyhedral stereosymmetric head which wraps nucleic acid and has a diameter of 70nm; the phage has a tail with a length of 120nm and has a tail sheath; a neck is connected with the head and the tail; and the phage belongs to myoviridae of caudovirales. The phage has a high titer of 10<12> pfu / mL and has good tolerance to temperature and pH value, and the titer still keeps 10<12>pfu / mL or above after continuous passage for 29 times. The phage has a good lysis effect on a host after being diluted by 10<6> times, and after the phage is fed, existence of the phage can be detected in heart, liver, lung, kidney, thymus and serum. Through animal experiments, the phage has a good effect in treating chicken salmonella diseases.
Owner:QINGDAO RUNDA BIOTECH
Novel esterase and its application
InactiveCN102732539AEfficient expressionHeat stableHydrolasesFermentationBiotechnologyEscherichia coli
The invention discloses DNA of a novel esterase. The novel esterase DNA has a nucleotide sequence as shown in SEQ ID NO.1 and an amino acid sequence as shown in SEQ ID NO.3. The invention also discloses an expression vector containing the novel esterase gene, a recombinant esterase and application of the recombinant esterase in degradation of homoserinelactone signal molecules. The novel esterasehas efficient soluble expression in an Escherichia coli expression system and good thermal stability, and has a strong degradation effect on homoserinelactone signal molecules.
Owner:SUN YAT SEN UNIV
Recombinant superoxide dismutase as well as coding gene and preparation method thereof
InactiveCN112725295AImprove thermal stabilityGood acid and alkali toleranceBacteriaOxidoreductasesDismutaseSuperoxide dismutases
The invention belongs to the field of organic matter separation and purification, and particularly relates to recombinant superoxide dismutase (recombinant SOD for short), a gene for encoding the recombinant SOD, a related expression vector, a transgenic cell line and host bacteria, and a preparation method of the recombinant SOD. The amino acid sequence of the recombinant SOD is shown as SEQ ID NO.4 in a sequence table. The gene sequence of the gene for encoding the recombinant SOD is shown as SEQ ID NO.3 in a sequence table. Tests prove that the recombinant SOD disclosed by the invention has excellent thermal stability, excellent acid-base tolerance and relatively good common preservative tolerance, and can be widely applied to the fields of medicines, cosmetics and foods.
Owner:ZHEJIANG UNIV OF TECH +2
Method for preparing recombinant-aspergillus niger glucose oxidase and application of recombinant-aspergillus niger glucose oxidase
ActiveCN104711273AHigh expressionImprove thermal stabilityFungiMicroorganism based processesSepharoseTrichoderma reesei
The invention discloses a method for preparing recombinant-aspergillus niger glucose oxidase and application of the recombinant-aspergillus niger glucose oxidase. Recombinant trichoderma reesei Tu6 delta tku70::Agod constructed by introducing an Agod gene into trichoderma reesei Tu6 delta tku70 can successfully express the recombinant-aspergillus niger glucose oxidase, the expression quantity is high, and the enzyme activity in fermentation broth can achieve 137 U / mL which is the highest level of the enzyme activity of shake flask fermentation in production of glucose oxidase strains at present. After the recombinant-aspergillus niger glucose oxidase in the fermentation broth is subjected to ni-sepharose purification, the detected enzyme activity can achieve 342 U / mL. The specific activity of the recombinant-aspergillus niger glucose oxidase is 155 U / mg protein. The recombinant-aspergillus niger glucose oxidase prepared by the method has good heat stability and good acid-alkali tolerance, the fermentation process is simple, transferring is not needed, raw materials are cheap and are easily obtained, and the cost is greatly reduced. The recombinant-aspergillus niger glucose oxidase can be widely applied to the fields of food and medicine.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Green environment-friendly peanut hull-containing fertilizer
InactiveCN106699392AUniform particle sizeEvenly dispersedCalcareous fertilisersAnimal corpse fertilisersBiotechnologyDolomite
The invention discloses a green environment-friendly peanut hull-containing fertilizer. The fertilizer comprises the following raw materials in parts by weight: 30-50 parts of peanut hull powder, 20-30 parts of furfural dreg, 20-40 parts of shell powder, 20-40 parts of plant ash, 15-25 parts of mulberry leaf, 20-40 parts of silkworm litter, 20-40 parts of river course silt, 4-10 parts of sodium carboxymethyl cellulose, 10-18 parts of gelatin carbonized wood powder compound, 4-10 parts of grass carbon, 3-7 parts of oil shale powder, 2-5 parts of illite powder, 4-12 parts of dolomite powder, 2-4 parts of calcium carbonate, 2-6 parts of sodium silicate, 4-8 parts of gypsum, 20-40 parts of urea, 30-40 parts of potash magnesium sulfate fertilizer, 25-45 parts of defluorinated phosphate fertilizer, 0.5-1 part of composite bacterium, 1-5 parts of soybean lecithin and 3-5 parts of coating agent. The fertilizer disclosed by the invention continuously provides multiple nutrient elements for crops, has excellent moisture retention and ventilation effects, is suitable for soil improvement and plant culture, and has the advantages of wide raw material sources and low price.
Owner:定远县文凡米业有限公司
Preparation method for gamma-polyglutamate water absorption material
The invention discloses a preparation method for a gamma-polyglutamate water absorption material. According to the method, gamma-polyglutamate adopted as a main raw material and caffeic acid-grafted chitosan oligosaccharide adopted as a biodegradable cross linking agent are used for preparing the gamma-polyglutamate hydrogel water absorption material in a water solution. The biodegradable cross linking agent is adopted to replace a common chemical crosslinking agent to prepare hydrogel, a reaction medium is not a toxic organic solvent any more, the safe and nontoxic gamma-polyglutamate is used as the main material, and the water-soluble degradable acrylic acid chitosan oligosaccharide is adopted as the cross linking agent to prepare the gamma-polyglutamate water absorption material. The real safe, nontoxic and biodegradable hydrogel is obtained as both the raw material and the medium are materials harmless to a human body, and the gamma-polyglutamate water absorption material is high in swelling ratio, excellent in mechanical property and improved in degradation property, and can be widely applied to disposable hygiene products, medicine carriers, medical supplies and the like.
Owner:新疆康润洁环保科技股份有限公司
Lactobacillus plantarum with inhibition effect on ETEC, and fermentation product and application thereof
ActiveCN111484958AImprove conversion ratePromote growthAntibacterial agentsBacteriaBiotechnologyAntibacterial activity
The invention provides lactobacillus plantarum with an inhibiting effect on ETEC as well as a fermentation product and application of the lactobacillus plantarum, and belongs to the technical field ofpreparation of probiotics and feed additives. The lactobacillus plantarum (Lactobacillus plantarum) provided by the invention is named as DMS LP1210, and the preservation number is GDMCC No.60717. The bacterium has the function of inhibiting ETEC, the bacteriostatic activity of the produced bacteriocin is still kept at 80% or above after the bacteriocin is subjected to heat treatment at 121 DEG Cfor 30 min, and the bacteriocin has tolerance to high temperature; 80% or above of the antibacterial activity is reserved when the pH value is 2-10, 90% or above of the antibacterial activity is reserved after pepsin, trypsin and protease K are used for treating the bacteria, the tolerance to the intestinal tract environment is good, the diarrhea rate is reduced, the feed conversion efficiency isimproved, the effect is safe and reliable, and the probiotics additive is suitable for being developed into feed probiotics additives.
Owner:泰安肽普德蛋白有限公司
Preparation method of L-arginine-based carbon dots and application of L-arginine-based carbon dots in lemon yellow detection
PendingCN114854407ALow costWide variety of sourcesNanoopticsNano-carbonArginineThin layer chromatographic
The invention relates to a preparation method of L-arginine-based carbon dots and an application of the L-arginine-based carbon dots in lemon yellow detection, which comprises the following steps: dissolving L-arginine and o-phenylenediamine in water, methanol, ethanol, n-propanol, acetone or N, N-dimethylformamide according to a molar ratio of 1: 20-1: 1 at normal temperature; transferring into a polytetrafluoroethylene high-temperature reaction kettle, and reacting at 170-210 DEG C for 2-12 hours; after the product is naturally cooled, centrifuging at a high speed, dialyzing for 24-72 hours by using a 500-5000Da dialysis bag, drying to obtain crude carbon dots, separating and purifying by using column chromatography, carrying out gradient elution by using an eluent, collecting a blue fluorescence part, and recovering the solvent under reduced pressure to obtain relatively pure carbon dots; continuously carrying out secondary gradient elution on the relatively pure carbon dots by using an eluent by adopting the column chromatography, and carrying out thin-layer chromatography detection to obtain fluorescent and ultraviolet dual-mode detection carbon dots; the fluorescence quenching mechanism of the prepared L-Arg-CDs and lemon yellow belongs to an inner filter effect, the optimal linear detection concentration range is 0-55 [mu] M, the detection limit LOD is 42.3 nM and is far lower than the detection limit 187.14 [mu] M provided by the national standard GB / T5009.35-2003, and the L-Arg-CDs and lemon yellow can be used as a rapid detection method of lemon yellow.
Owner:KUNMING UNIV
Environment-tolerant aeromonas hydrophila bacteriophage ZPAH34 and application
PendingCN114703150AEffective preventionEffective therapeuticAntibacterial agentsBacteria material medical ingredientsBiotechnologyPseudomonas hydrophila
The invention belongs to the technical field of microorganisms, and particularly relates to an environment-tolerant aeromonas hydrophila bacteriophage ZPAH34 and application, and the preservation number of the aeromonas hydrophila bacteriophage ZPAH34 is CCTCC (China Center For Type Culture Collection) NO: M 2022141. The aeromonas hydrophila bacteriophage provided by the invention is separated from the natural environment, has the characteristics of high specificity, strong splitting ability, better temperature and pH tolerance and the like, and can have stronger inhibition and removal effects on a biofilm formed by aeromonas hydrophila. As a bacterial control means, the bacteriophage can reduce the viable count of aeromonas hydrophila in lettuce and fish slices. Meanwhile, the phage provided by the invention can effectively prevent and treat infection caused by aeromonas hydrophila on zebra fish, and has a wide application prospect in aquaculture. The research provides a scientific basis for bacteriophage application research, and also provides a theoretical reference for biological prevention and control strategy development of aeromonas hydrophila.
Owner:HUAZHONG AGRI UNIV
Preparation method and application of layered Steiner network structure fiber membrane
ActiveCN111220606AHigh sensitivityGood acid and alkali toleranceMaterial analysis by observing effect on chemical indicatorNon-woven fabricsTriethanolamineExplosive detection
The invention discloses a preparation method and an application of a layered Steiner network structure fiber membrane. The layered Steiner network structure fiber membrane is prepared from the following polymers: polyvinyl alcohol, chitosan, polyvinylpyrrolidone, polyacrylic acid, polypropylene cellulose, polycarbonate and polyimide, the following pore-foaming agent: dichloromethane, polyvinyl alcohol, dimethyl sulfoxide, ammonium bicarbonate, sodium bicarbonate, acetone or tetrahydrofuran, the following cross-linking agents: glutaraldehyde, methylene bisacrylamide, triethanolamine, polyethyleneimine or diethylenetriamine, the following catalysts: hydrochloric acid, sulfuric acid or phosphoric acid and a solvent being deionized water. When the layered Steiner network structure fiber membrane prepared by adopting the preparation method is applied to colorimetric explosive detection, the sensitivity of colorimetric detection can be improved in two aspects of reaction time and response degree, and the layered Steiner network structure fiber membrane has the advantages of being sensitive in reaction, instant in result display, simple in operation and use method and the like. Moreover,various non-standard explosives and standard explosive raw materials are detected and identified according to different explosive detection reagents loaded in the fiber membrane, so that the layered Steiner network structure fiber membrane has universality.
Owner:XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
Method for preparing recombinant-penicillium amagasakiense glucose oxidase and application of recombinant-penicillium amagasakiense glucose oxidase
ActiveCN104711274AImprove thermal stabilityHigh expressionFungiMicroorganism based processesUltrafiltrationSepharose
The invention discloses a method for preparing recombinant-penicillium amagasakiense glucose oxidase and application of the recombinant-penicillium amagasakiense glucose oxidase. Recombinant trichoderma reesei Tu6 delta tku70::Pgod constructed by introducing a Pgod gene into trichoderma reesei Tu6 delta tku70 can successfully express the recombinant-penicillium amagasakiense glucose oxidase, the expression quantity is high, and the enzyme activity in fermentation broth can achieve 28 U / mL. The recombinant-penicillium amagasakiense glucose oxidase in the fermentation broth is subjected to ni-sepharose purification, the enzyme activity in a protein solution obtained by ultrafiltration concentration can achieve 330 U / mL, and the specific activity of the recombinant-penicillium amagasakiense glucose oxidase is 407 U / mg protein. The recombinant-penicillium amagasakiense glucose oxidase prepared by the method has good heat stability and good acid-alkali tolerance, the fermentation process is simple, transferring is not needed, raw materials are cheap and are easily obtained, and the cost is greatly reduced. The recombinant-penicillium amagasakiense glucose oxidase can be widely applied to the fields of food and medicine.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Bacillus amyloliquefaciens and influence of bacillus amyloliquefaciens on soil nutrient content and enzyme activity
InactiveCN112322542AImprove high temperature resistanceGood acid and alkali toleranceBiocidePlant growth regulatorsMicrobial agentMicrobiological culture
The invention is applicable to the technical field of microbial breeding, and provides bacillus amyloliquefaciens. The bacillus amyloliquefaciens is bacillus amyloliquefaciens PLBS010 and is preservedin China General Microbiological Culture Collection Center on December 17, 2018, and the preservation number is CGMCC NO.16950. The invention further provides an application of the bacillus amyloliquefaciens to soil nutrient content and enzyme activity. Therefore, the bacillus amyloliquefaciens PLBS010 disclosed by the invention has better high-temperature tolerance, acid-base tolerance, salt tolerance and pathogenic fungi inhibition, and by applying a microbial agent containing the bacillus amyloliquefaciens PLBS010 to soil, soil nutrient imbalance and soil-borne disease aggravation can be avoided, and crop growth and development and stable, healthy and sustainable development of agricultural production are promoted.
Owner:青岛普罗百世生物科技有限公司
Preparation and Application of Hollow Spherical Mesoporous Silica/Chloroperoxidase Nanoreactor
ActiveCN105907743BEasy to prepareMild reaction conditionsWater contaminantsOn/in inorganic carrierNanoreactorPeroxidase
The invention discloses preparation and application of a hollow spherical-segment-shaped mesoporous silica / chloroperoxidase nanoreactor. According to the nanoreactor, tetraethoxysilane serves as a silicon source, P123 serves as a template agent, and a compound of n-decane and 1,3,5-trimethylbenzene serves as an additive; a hydrothermal synthesis method is utilized for preparing hollow spherical-segment-shaped mesoporous silica firstly, then the mesoporous silica serves as a fixed carrier for fixedly carrying chloroperoxidase. The preparation method is simple, reaction conditions are mild, the obtained mesoporous silica is in a hollow spherical segment shape, the specific surface area is large, the mesoporous diameter range is about 14-18 nm, unimolecular fixation of the chloroperoxidase can be realized, and certain turnover space is reserved for the chloroperoxidase. The hollow spherical-segment-shaped mesoporous silica / chloroperoxidase nanoreactor shows good degradation capacity on alizarin red, the degradation rate still can reach 100% after 8 times of use, the high temperature to which the nanoreactor is resistant is increased to about 50 DEG C, and the acid-base resistance is also improved.
Owner:SHAANXI NORMAL UNIV
A kind of preparation method and application of recombinant Aspergillus niger glucose oxidase
ActiveCN104711273BImprove thermal stabilityThe fermentation process is simpleFungiMicroorganism based processesMicrobiologyAspergillus niger
The invention discloses a preparation method and application of recombinant Aspergillus niger glucose oxidase. The recombinant Trichoderma reesei Tu6△tku70::Agod constructed by introducing the Agod gene of the present invention into Trichoderma reesei Tu6△tku70 can successfully express recombinant Aspergillus niger glucose oxidase, and the expression level is relatively high. The activity can reach 137U / mL, which is the highest level of shake flask fermentation enzyme activity among glucose oxidase-producing strains. The enzyme activity measured after the recombinant Aspergillus niger glucose oxidase in the fermentation broth was purified by a nickel column can reach 342U / mL, and the specific activity of the recombinant Aspergillus niger glucose oxidase is 155U / mg protein. The recombinant Aspergillus niger glucose oxidase prepared by the method of the present invention has good thermal stability and good acid-base tolerance, simple fermentation process, no transfer, cheap and easy-to-obtain raw materials, greatly reduced cost, and can be widely used Applied in the field of food and medicine.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
A kind of low-salt kimchi micro-ecological additive and preparation method thereof
ActiveCN106754506BImprove securityNo inhibitionBacteriaMicroorganism based processesBiotechnologyEscherichia coli
The invention belongs to the field of food, in particular to the technical field of kimchi fermentation in food, and specifically relates to a low-salt kimchi microecological additive and a preparation method thereof. The low-salt kimchi micro-ecological additive includes the strain Lactobacillus pentosus, which is classified as Lactobacillus pentosus PCYSWX-5, and the strain was stored in the General Microorganism Center of China Microbiological Culture Collection Management Committee on July 15, 2016, and the preservation number is CGMCCNo.12793 . The micro-ecological additive prepared in the present invention can inhibit or kill common pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Enterobacter sakazakii, Listeria monocytogenes, Salmonella, and has no inhibitory effect on main fermentation bacteria , improve the safety of eating pickles; can strongly inhibit or kill the nitrate-reducing bacteria in low-salt pickles in the early stage of fermentation, so that the nitrite content in low-salt pickles is lower than the national standard.
Owner:SICHUAN DONGPO CHINESE PAOCAI IND TECH RES INST
Thermophilic C-phycocyanin and method for extracting thermophilic C-phycocyanin from synechococcus cyanobacteria
InactiveCN107353337AImprove thermal stabilityGood acid and alkali toleranceBacteriaMicroorganism based processesSynechococcusPhylum Cyanobacteria
The invention discloses thermophilic C-phycocyanin. The thermophilic C-phycocyanin has an amino acid sequence which is shown as a first sequence, and amino acid at three or less characteristic positions of the 21th, 28th, 33th, 37th, 42th and 145th positions of amino acid sequence can be adjusted. The invention further provides a method for extracting the thermophilic C-phycocyanin from synechococcus cyanobacteria. According to the method for extracting the thermophilic C-phycocyanin from synechococcus cyanobacteria, the obtained thermophilic C-phycocyanin has good thermal stability and acid-base tolerance; compared with the phycocyanin which is extracted through spirulina and the like in the prior art, the thermophilic C-phycocyanin has good thermal stability and acid-base tolerance so that the thermophilic C-phycocyanin can have wider application range in the industrial application and shows more remarkable application advantages.
Owner:PEKING UNIV SHENZHEN GRADUATE SCHOOL
A method for preparing gamma-polyglutamic acid water-absorbing material
The invention discloses a preparation method for a gamma-polyglutamate water absorption material. According to the method, gamma-polyglutamate adopted as a main raw material and caffeic acid-grafted chitosan oligosaccharide adopted as a biodegradable cross linking agent are used for preparing the gamma-polyglutamate hydrogel water absorption material in a water solution. The biodegradable cross linking agent is adopted to replace a common chemical crosslinking agent to prepare hydrogel, a reaction medium is not a toxic organic solvent any more, the safe and nontoxic gamma-polyglutamate is used as the main material, and the water-soluble degradable acrylic acid chitosan oligosaccharide is adopted as the cross linking agent to prepare the gamma-polyglutamate water absorption material. The real safe, nontoxic and biodegradable hydrogel is obtained as both the raw material and the medium are materials harmless to a human body, and the gamma-polyglutamate water absorption material is high in swelling ratio, excellent in mechanical property and improved in degradation property, and can be widely applied to disposable hygiene products, medicine carriers, medical supplies and the like.
Owner:新疆康润洁环保科技股份有限公司
Salmonella phage composition and application thereof
The invention provides a salmonella phage composition and application thereof, and belongs to the technical field of biology. The salmonella bacteriophage composition provided by the invention comprises at least one of a bacteriophage GSP162, a bacteriophage GSP193, a bacteriophage GSP001 and a bacteriophage GSP032. The salmonella bacteriophage composition provided by the invention plays a role in four receptors of salmonella lipopolysaccharide (LPS) O antigen, LPS core polysaccharide, outer membrane protein BtuB and outer membrane protein TolC respectively. The four bacteriophages screened by the invention have good temperature tolerance and acid-base tolerance, can be used for cracking salmonella of various different serotypes, and are wide in cracking spectrum; an in-vitro bacterial lysis experiment shows that compared with the use of a single phage, the phage cocktail can effectively delay the appearance of an anti-phage strain, and the synergistic effect is obvious. A mouse intestinal sterilization test shows that the bacteriophage composition disclosed by the invention can be used for effectively reducing the salmonella carrying amount in cecum.
Owner:HUAZHONG AGRI UNIV
Microporous membrane osmotic emulsification method for preparing gelatin microspheres immobilized alcohol dehydrogenase
InactiveCN104762289BEasy to fixGood acid and alkali toleranceOn/in organic carrierParaffin waxOil phase
The invention relates to a preparing method for a gelatin microsphere of fixed alcohol dehydrogenase by micro-porous membrane permeation and emulsification. The method comprises the following steps that solid gelatin and alcohol dehydrogenase are dissolved in deionized water, and a gelatin / alcohol dehydrogenase solution called as a water phase is prepared; oil soluble emulsifier span-80 is added into liquid paraffin, and liquid paraffin oil called as an oil phase is prepared; a micro-porous membrane is immersed into the liquid paraffin oil, according to the volume ratio of the gelatin / alcohol dehydrogenase solution and the liquid paraffin oil, the gelatin / alcohol dehydrogenase solution is pressed to penetrate through the micro-porous membrane and enter into the liquid paraffin oil, and W-shaped or O-shaped emulsion is formed by the emulsification; glutaraldehyde is added into the W-shaped or O-shaped emulsion, and after cross linking, solidifying, washing and drying are carried out, the gelatin microsphere of the fixed alcohol dehydrogenase is obtained. The preparing method for the gelatin microsphere of the fixed alcohol dehydrogenase by the micro-porous membrane permeation and emulsification has the advantages that the preparing condition is warm, the preparing technology is simple and easy to carry out, the granularity of the prepared microsphere is even, the size is controllable, the storing stability is good, the leakage rate of the alcohol dehydrogenase in the using process is low, and the anti-acid-base environment capacity is strong.
Owner:TIANJIN UNIV OF SCI & TECH
Preparation method and application of a layered Steiner network structure fiber membrane
ActiveCN111220606BGood acid and alkali toleranceHigh sensitivityMaterial analysis by observing effect on chemical indicatorNon-woven fabricsSodium bicarbonateO-Phosphoric Acid
Owner:XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
A kind of preparation method and application of recombinant Penicillium amagasaki glucose oxidase
ActiveCN104711274BImprove thermal stabilityHigh expressionFungiMicroorganism based processesSepharoseTrichoderma reesei
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
A stable and efficient Salmonella virulent phage rdp-sa-21004 and its application
ActiveCN113430176BHas a strong cracking effectConvenient sourceAntibacterial agentsBiocideEscherichia coliBacteriophage
The invention discloses a stable and efficient Salmonella virulent phage RDP-SA-21004, the preservation number is CGMCC No. 22483. After the whole gene is sequenced, it is found that it does not have virulence genes and lysogenic genes; Stored for 10 months under conditions, its activity remains at 10 8 Above pfu / mL, it has strong stability; it can efficiently infect host bacteria, and its optimal multiplicity of infection is 0.1; it has a broad spectrum of ability to kill Salmonella, and also has a certain ability to lyse Escherichia coli. The phage can be used alone or in combination with other Salmonella phages and probiotics, which can effectively treat, prevent and reduce poultry salmonellosis.
Owner:RECOM QINGDAO BIOTECH CO LTD
Method for analyzing etimicin
ActiveCN101614712BEfficient separationGood acid and alkali toleranceComponent separationInjection volumeDivinylbenzene
The invention relates to a method for detecting etimicin and related substances thereof. The method adopts high performance liquid chromatography; chromatographic conditions are that: polystyrene-divinylbenzene polymer chromatographic column is MKF-RP-ETI (the particle size is between 4 and 10mu m, the column specification is (100-300)*(8.0-4.6)mm (I.D.)); the volume ratio of compositions of a mobile phase is that the volume ratio of an acetate buffer solution (the pH value is between 4 and 7) with a concentration of between 0.10 and 0.50mol / L to acetonitrile is 90-100: 10-0; the flow rate is between 0.8 and 1.2mL / min; a detector is a refractive index detector; and the injection volume is between 10 and 20muL. The method can finish the on-line detection on the etimicin and the related substances by using the prior HPLC instrument and refractive index detector; and the method is simple, quick and accurate.
Owner:HAINAN AIKE PHARMA
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