753results about How to "The fermentation process is simple" patented technology

A process for obtaining a product sugar stream from cellulosic biomass is disclosed. In one process, the cellulosic biomass is pretreated at a pH between about 0.4 to 2.0 by adding one or more than one acid to produce a pretreated cellulosic biomass comprising acetic acid. One or more than one base is then added to the pretreated cellulosic biomass to adjust the pretreated cellulosic biomass to a pH of about 4.0 to about 6.0 to produce a neutralized cellulosic biomass comprising inorganic salt and acetate salt. The neutralized biomass is then hydrolyzed by cellulase enzymes to produce a crude sugar stream. Insoluble residue is separated from the crude sugar stream and the resulting clarified sugar stream is treated using ion exclusion chromatography at about pH 5.0 to about 10.0 to produce one or more raffinate streams and a product stream. The raffinate stream comprises inorganic salts and acetate salts, and the product stream comprises sugar. The product stream may then be fermented or otherwise further processed. In an alternate process, a product sugar stream is obtained from a crude sugar stream that is produced from conversion of cellulosic biomass to sugar. The cellulosic biomass may be produced using any suitable method. In this process the crude sugar stream is treated using ion exclusion chromatography at about pH 5.0 to about 10.0 to produce one or more than one raffinate stream comprising sulfate and acetate salts, and a product stream comprising sugar, and the product sugar stream is obtained.

The invention relates to a process for obtaining a product sugar stream from a cellulosic biomass, comprising pretreating the cellulosic biomass to hydrolyze a portion of the cellulose and hemicellulose to produce glucose, acetic acid and a sugar monomer, adding base to the pretreated cellulosic biomass to produce inorganic salt and acetate salt; hydrolyzing the neutralized cellulosic biomass with cellulase enzymes and separating insoluble residue from the resulting sugar stream; treating the clarified sugar stream using exclusion chromatography at a pH of 5 to 10 to produce a product sugar stream.

The invention relates to a self-propelled turning device for sludge compost and a treatment method and belongs to environmentally-friendly solid waste treatment. The method comprises that: a mixing step, an aerobic compost period, a thoroughly decomposing step, a processing and compost production step and an odor treatment step, wherein the mixing step aims to adjust the moisture and the carbon nitrogen rate of dewatered sludge, improve the loosening degree, increase a contact area with air and contributes to aerobic fermentation; in the aerobic compost period, the oxygen is supplied through forced ventilation, and organic matters in materials begin to ferment under the action of aerobes; the thoroughly composting step comprises that: the sludge subjected to compost fermentation is not thoroughly decomposed, and is required to continuously undergo a thoroughly decomposing and ageing stage, namely secondary fermentation; the processing and compost production step comprises that: the sludge compost is processed according to fertilizer use and market demand to serve as a product for sales so as to improve overall economic efficiency; and the odor treatment step comprises that: odor is generated in the process of aerobic compost and can be discharged after deodorization treatment. In recent years, solid wastes have become important environmental problems limiting urban development. The composting treatment is a way to utilize solid waste resources and is gradually taken into account at home and abroad.

The invention discloses a streptomyces corchorusii strain NF0919, which is collected in China General Microbiological Culture Collection Center, and has the collection number CGMCC No. 3968. The strain can be used for preventing and treating rhizoctonia cerealis, rice false smut, rice sheath blight disease and other various plant diseases.

The present invention provides a method for converting lignocellulosic material into biofuel. In particular embodiments, the method comprises pre-treating lignocellulosic material by dissolving the material in ionic liquids. The pretreated lignocellulosic material can be isolated, such as by precipitation with a regenerating solvent (e.g., water), and be used directly in the formation of biofuel, including undergoing hydrolysis to form sugar and fermentation to form fuel, such as bioethanol. The ionic liquid can be recycled for further use, such as by evaporation of the water introduced during precipitation, and the recycling provides a route to a hemicellulose rich fraction and an ionic liquid of consistent quality and wood dissolution characteristics. The recovered hemicelluloses are of significant utilization potential toward commodity and specialty applications.

The invention discloses a processing method of flower fragrance type kung fu black tea. The method comprises the steps of fresh leaf picking, sun drying, fine manipulation of green tea leaves, quick-freezing withering, unfreezing, rolling, fermentation, first firing, full firing, fragrance increasing, vacuum packaging and the like. The technology is improved for solving the problems that when Lingyun white tea variety black tea is processed, taste is light in freshness and sweetness, fragrance is light and style and variety are monotonous. The processing method comprises the specific steps that tea shoots with two leaves and a bud or with three leaves and a bud of Lingyun white tea trees are picked as raw materials, and technological parameters of sunlight withering, fine manipulation of green tea leaves, freezing withering, rolling, fermentation, full-fire drying, drying and fragrance increasing are enhanced so as to improve a traditional processing method, so that the product has the quality style characteristics that strips are compact and uniform, color and luster are black and smooth, the soup color is orange red and bright, the fragrance is rich and lasting, the taste is mellow and refreshing and leave bottoms are red, bright and uniform, and the black tea has obvious nectar fragrance besides sweet fragrance, and meets requirements of different consumers.

The invention discloses a phellinus igniarius mycelium liquid fermentation method by utilizing a fungal elicitor for improving the yield of flavone of phellinus igniarius, which comprises the steps of: inoculating a fungal elicitor strain for activated culture, and then inoculating and culturing a fungal block to obtain a fungal elicitor mycelium through filtration; then drying, grinding and crushing the fungal elicitor mycelium, adding the crushed fungal elicitor mycelium into ethanol for soaking, filtering the obtained solution, collecting filter residue, orderly adding a mixed solution of chloroform and normal butanol, acetone and water into the filter residue for washing, adding trifluoroacetic acid into a dry substance after the air-drying, performing boiling water bath for 0.5 to 2 hours, then filtering the obtained solution, taking a filtrate for neutralization, standing the filtrate, using a microporous filtering film with the pore diameter between 0.4 and 5mu m to filter the filtrate for sterilization to obtain the fungal elicitor, and cryopreserving the fungal elicitor at the temperature of between 10 and 20 DEG C below zero; inoculating a phellinus igniarius strain for activated culture, and then inoculating a fungal block for fermentation culture; and adding the fungal elicitor into the phellinus igniarius to continue culturing to obtain a phellinus igniarius mycelium within 1 to 5 days of liquid fermentation culture of the phellinus igniarius. The method has simple process, low cost and remarkable effect, can greatly improve the content of the flavone of phellinus igniarius cells, and is a production method with industrialization prospect.

A solid-liquid filtering method and system which comprises a thick band (7) of assembled filaments prepared by closely arranging paralleled monofilaments of predetermined length, a means for fixing (11) one end portion of said band and a network support (4) for keeping said band in a plane situation at proper location when said band is forced to turn in a regular direction, one end portion of said monofilaments being fixed and the other end portion being floated.

The invention belongs to the field of fermentation engineering and discloses corynebacterium glutamicum and a method for preparing L-ornithine and salts thereof by using the same. The corynebacterium glutamicum is corynebacterium glutamicum 1006 with the collection number of CGMCCNo.3663. The method comprises the following steps of: culturing the corynebacterium glutamicum in a culture medium containing carbon sources, nitrogen sources and inorganic salts at the temperature of between 28 and 30 DEG C to generate L-ornithine fermentation liquor; removing the thalli of the fermentation liquor by using ceramic membranes of 1 to 15 ten thousand Dalton; separating the fermentation liquor by using cation exchange resins to obtain L-ornithine solution; and decoloring and crystallizing the L-ornithine solution by a hydrochloric acid and ethanol method to obtain L-ornithine hydrochloride. The method has the advantages of simple thallus cultivation and contribution to industrialized mass production. The accumulation of the L-ornithine prepared from the corynebacterium glutamicum reaches 35 to 45g / L.

The invention discloses a method utilizing fermentation to improve the gamma-aminobutyric acid content of brown rice, and aims to solve the problems that the gamma-aminobutyric acid content is low in brown rice, and the conventional fermentation technology is complicated, is not suitable for increasing the gamma-aminobutyric acid content in brown rice, and cannot increase the taste and nutritional and healthcare value of brown rice. The method grafts a lactobacillus P-14 strain having a high yield of gamma-aminobutyric acid (GABA) onto brown rice or brown rice, which has been added with monosodium glutamate, to carry out fermentation so as to obtain fermented brown rice rich in GABA. The P-14 strain is isolated from traditional pickled vegetables, the fermentation raw material is sterilized brown rice, and the fermentation products can reach the food safety grade. Through the fermentation treatment, the nutrition and healthcare value of the brown rice are both improved in different levels, and the fermented brown rice can satisfy the nutritional and healthcare requirements on food.

Improvements in plasmid DNA production technology are needed to insure the economic feasibility of future DNA vaccines and DNA therapeutics. General methods are described, by means of which it is possible to dramatically increase plasmid DNA productivity in a fermentor. These processes feature Fed-batch fermentation strategies, combined with novel growth and induction phase temperature shifts.