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Method for producing 2,3-butanediol by using starch raw materials

A butanediol and starch technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of reducing fermentation production costs, rapid regeneration cycle, and improving utilization efficiency

Inactive Publication Date: 2009-10-28
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Production of 2,3-butanediol by direct fermentation of starch raw materials is rarely reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: Utilize Klebsiella pneumoniae (Klebsiella pneumoniae) ATCC 8724 to take cornstarch as raw material to produce 2,3-butanediol in 5 liters of fermentors step-by-step saccharification fed-batch fermentation

[0041] (1) Preparation of cornstarch treatment and fed-batch fermentation medium for step-by-step saccharification:

[0042] Add water to 1200g cornstarch to adjust the starch slurry to 2.5L, adjust the pH to 5.5, add α-amylase according to the enzyme amount of 10U / g dry weight of starch, and cook at 95°C for 2 hours to obtain cornstarch liquefaction liquid; cooling the corn starch liquefaction solution to 55°C, adjusting the pH of the liquefaction solution to 4.0, adding glucoamylase according to the enzyme amount of 1000U / g dry weight of starch, and treating it at 55°C for 2 hours to obtain a corn starch saccharification solution, The measured glucose concentration was 452g / L, and the saccharification solution was sterilized at 115°C for 20 minutes, an...

Embodiment 2

[0046] Example 2: Utilize Klebsiella pneumoniae (Klebsiella pneumoniae) ATCC 8724 to produce 2,3-butanediol in 5 liters of fermentor with synchronous saccharification fed-batch fermentation with cornstarch as raw material

[0047] (1) Preparation of cornstarch treatment and simultaneous saccharification fed-batch fermentation medium:

[0048] Add water to 1200g of cornstarch to make a starch slurry, adjust the volume to 2.5L, adjust the pH to 6.5, add α-amylase according to the amount of enzyme added at 2U / g dry weight of starch, and cook at 90°C for 4 hours to obtain cornstarch liquefaction liquid, sterilized at 115°C for 20 minutes, and used as a carbon source after cooling to prepare a fermentation medium for synchronous saccharification fed-batch fermentation;

[0049] Fermentation medium suitable for synchronous saccharification fed-batch fermentation, the formula is: the carbon source is cornstarch liquefaction, and its dosage is 150mL / L; the remaining ingredients are: (...

Embodiment 3

[0052] Example 3: Utilize Klebsiella pneumoniae (Klebsiella pneumoniae) SDM CCTCC M 208097 to produce 2,3-butanediol in a 5 liter fermenter with synchronous saccharification fed-batch fermentation using cassava flour

[0053] (1) Preparation of cassava flour treatment and simultaneous saccharification fed-batch fermentation medium:

[0054] Add water to 540g of cassava flour to adjust the starch slurry to 1.2L, adjust the pH to 6.5, add α-amylase according to the amount of enzyme added to 5U / g dry weight of starch, cook at 90°C for 1 hour, and obtain cassava flour liquefaction liquid, sterilized at 115°C for 20 minutes, and used as a carbon source after cooling to prepare a fermentation medium for synchronous saccharification fed-batch fermentation;

[0055] The fermentation medium suitable for simultaneous saccharification fed-batch fermentation, the formula is: the carbon source is cassava flour liquefaction liquid, and its dosage is 170mL / L; the remaining ingredients are: (...

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PUM

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Abstract

The invention discloses a method for producing 2,3-butanediol by using starch raw materials, which comprises the following steps: subjecting the starch raw materials to treatment by alpha-amylase and maltogenic amylase; and producing the 2,3-butanediol by carrying out separate hydrolysis and fermentation or simultaneous sacchrification and fermentation under an aseptic condition, using saccharification liquid or liquefaction liquid as a fermentable substrate and using Klesiella pneumoniae as a fermentable strain. Finally the concentration of the 2,3-butanediol reaches 43 to 112g / L, and the final concentration of acetoin and the final concentration of the 2,3-butanediol reach 45 to 121g / L. The method has the advantages of using the starch raw materials to produce a high-value platform compound by fermentation, reducing the raw material cost of the production of the 2,3-butanediol by the fermentation and establishing the optimal fermentation condition for the production of the 2,3-butanediol by the sacchrification and fermentation of the starch raw materials. The production of the 2,3-butanediol by the simultaneous sacchrification and fermentation of the invention can save energy and equipment investment, reduce fermentation period and improve production efficiency.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for producing 2,3-butanediol by using starch raw materials. Background technique [0002] 2,3-butanediol (2,3-butanediol) is a high value-added platform compound with a wide range of applications. 2,3-Butanediol is a colorless and odorless chiral compound with 3 configurations. The boiling point is 180-184°C, and the freezing point is low. D-2,3-butanediol (-60°C) can be used as an antifreeze agent. 2,3-Butanediol has a high octane number and can be used to produce high-grade aviation kerosene, which is a very valuable liquid fuel. Dehydration can be converted into industrial solvent methyl ethyl ketone, methyl ethyl ketone can be used as a fuel additive, and can be used as a solvent in resin and paint industry. Further dehydration of methyl ethyl ketone can obtain 1,3-butadiene, which can be used as a substrate in the synthetic rubber industr...

Claims

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Application Information

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IPC IPC(8): C12P7/18C12R1/22
Inventor 马翠卿许平王爱龙王钰李理想唐鸿志
Owner SHANDONG UNIV
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