44results about How to "Reduce the cost of fermentation production" patented technology

The invention belongs to the field of fermentation engineering and relates to a method for extracting DHA crude oil. Specifically, the method includes the following steps of 1, conducting dehydrationtreatment on microbial fermentation liquid used for generating DHA; 2, flexibly squeezing a product obtained in step 1 to obtain the DHA crude oil. The DHA prepared by means of the method has the advantages of high yield and high purity, and the method is favorable for large-scale industrial production of the DHA.

The invention discloses a prodigiosin-producing bacterium and its production method and application. The prodigiosin-producing bacterium is Serratia marcescens BWL1001, the preservation number is CGMCC No.18953, and it produces prodigiosin The method comprises: the preparation of seed liquid; the seed liquid is transferred to the fermentation medium with soybean oil as carbon source by the ratio of 1% (v / v), 28 ℃ constant temperature shaker culture 24-48 hours obtains fermentation liquid Centrifuge the fermentation broth, collect the upper oil phase, add 1 to 3 times the volume of acidic methanol with pH=3 to the oil phase, oscillate and mix, let stand for 30 minutes, centrifuge, collect the acidic methanol phase, remove the solvent to obtain bleeding A red pigment product, the prodigiosin exhibits a good inhibitory effect on the growth of Microcystis aeruginosa. The bacterial strain used in the invention has fast growth rate, short fermentation cycle, high prodigiosin output and low production cost, and plays an important role in promoting the industrial application of prodigiosin.

The invention relates to the field of gene engineering, particularly a high-specific-activity endo-xylanase NPWXynB, and a gene and application thereof. Orthogenesis and site-directed mutagenesis are utilized to perform molecular modification on endo-xylanase XynB derived from rumen fungus Neocallimastix patriciarum to obtain the high-specific-activity xylanase NPWXynB. In the amino acid sequence, the 58th site Y is substituted by F, the 78th site M is substituted by Y, the 94th site Y is substituted by S, the 143rd site V is substituted by L, the 145th site E is substituted by R, and the 182nd site D is substituted by R. The specific activity of the endo-xylanase mutant NPWXynB provided by the invention is 11500 U / mg under the condition of 37 DEG C, which is enhanced by 82.5% as compared with the specific activity of the original endo-xylanase XynB. The maximum enzyme activity of the NPWXynB-gene-containing recombinant engineering bacterium can reach 153000 U / mL, and the production cost is greatly lowered.

The invention provides a novel photosynthetic organism hydrogen generation process based on the oscillation-light-filling strategy. Specifically, the invention provides a hydrogen generation method utilizing photosynthetic bacteria. The method comprises the following steps: (1) culturing photosynthetic bacteria capable of generating hydrogen under the nonstatic state and the irradiation of initial incident light with the light intensity I1, wherein the light intensity I1 of the initial incident light satisfies the following conditions: (i) I1>I0, I0 is the optimal light intensity of the initial incident light during static cultivation; (ii) under the light intensity I1, the actual light intensity I1' in at least 70% of area (or volume) of the culture system is larger than or equal to the actual light intensity I0' in the same area (or volume) during static cultivation; and (2) separating the generated hydrogen from the culture system. By using the method of the invention, hydrogen generation efficiency of photosynthetic bacteria can be effectively improved, hydrogen generation period can be shortened and high substrate conversion efficiency can be maintained.

The invention discloses a pretreatment method for producing butanol by fermentation of bagasse. The pretreatment method comprises the following steps of mechanical grinding, liquid-state hot-water treatment, microwave treatment, microbial decomposition, ammonia treatment and enzymolysis treatment, after the steps are performed, the decomposition products in each step can be mixed and subjected to step-by-step saccharification and fermentation and simultaneous saccharification and fermentation. By the pretreatment method, the contents of major inhibitors furancarboxaldehyde and hydroxymethyl furfural can be controlled below the concentration without affecting the growth of fermentative bacteria and other inhibitors such as glucuronic acid, p-coumaric acid, syringic acid and ferulic acid are not basically produced. Since the detoxification purification treatment of the pretreated decomposition products is not needed and the pretreated decomposition products are directly subjected to biological fermentation to produce butanol, the pretreatment method has the advantages that the production of inhibitors can be controlled, the cost is low, the yield is high and the environment friendliness is achieved.

The invention provides a microorganism fermentation method for arachidonic acid. The microorganism fermentation method comprises the following steps of: inoculating ordinary mortierella strains which is used for producing the arachidonic acid on a PDA (Potato Dextrose Agar) slope; stewing a solid culture medium at a temperature of 80-100 DEG C for 20-30 minutes, and airing to dry and scatter the solid culture medium without clusters, wherein the solid culture medium includes liquid components and solid components, the solid components are selected from one or more of wheat bran, rice and millet, and are mixed with the liquid components at a ratio of 10:7; adding 15-25mL sterile water to a fresh arachidonic acid-producing ordinary mortierella strain slope to wash spores so as to prepare spore suspension, uniformly inoculating the spore suspension into the solid culture medium, cultivating the solid culture medium at a temperature of 24-26 DEG C for 2-3 days as a mother culture medium for primary fermentation; and washing the spores on surfaces of solid seeds by using 200-300ml sterile water to prepare the spore suspension, inoculating the spore suspension into a primary fermentation tank based on an inoculation amount of 5-10% by volume, cultivating the primary fermentation tank at a changed temperature through controlling temperatures before and after the fermentation, and adding auxiliary materials for two times during the fermentation process so as to promote the growth of thallus and synthesis of grease. The microorganism fermentation method has the advantages of shortened period and lowered cost, and can be used for effectively improving fermentation level of the arachidonic acid.

The invention discloses a method for drying mycelia. The method includes the following steps that (1) initial mycelia with the water content of 75%-80% are fed into a distributing bin of microwave hot air composite drying equipment to be mechanically distributed; (2) the distributed mycelia are conveyed into a drying chamber of the microwave hot air composite drying equipment and dried through hot air and microwaves at the same time, and meanwhile moisture in the drying chamber is exhausted through a dehumidifier; after being dried to be with the water content of 20%-25%, the mycelia are discharged, and the discharge temperature is 80-90 DEG C; (3) the mycelia are fed into the drying chamber of the hot air drying equipment to be dried, and meanwhile moisture in the drying chamber is exhausted through the dehumidifier; when dried to be with water content lower than 10%, the mycelia are discharged, and the discharge temperature is 60-70 DEG C; (4) the mycelia are smashed, screened and graded; and (5) materials of different grades are inspected and packaged, and dried finished mycelium are obtained. The process is short in drying period, simple in procedure, high in efficiency and free of pollution.

A method for culturing red-hair yeast with shrimpanin synthesis accelerator. Use shrimpanin synthesis former-rich fruits, vegetables and tea juice such as fresh orange, spinach, tea leaf, etc as materias, and prepare shrimpanin synthesis accelerator by pressing juice or immersion. Add the shrimpanin synthesis accelerator in red-hair yeast medium at different fermention time; ferment for some time; collect yeast cell. Also we can furtherly do cell wall breaking treatment and extrat shrimpanin from the cell with acetone. The red-hair yeast achieved has high shrimpanin content, and the shrimpanin content can be advanced largely just by adding some shrimpanin synthesis accelerator. It has the advantages of investment little, raw materials cost little, and so on. Both the achieved red-hair yeast and the shrimpanin extracted from it can be used as feedingstuff additive for top-grage aquatic products and birds, and can make the culturing products have high commondity value.