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Probiotics prepared from bacillus coagulans and lactobacillus plantarum as well as preparation method thereof

A technology of Bacillus coagulans and Lactobacillus plantarum, applied in the field of feed microorganisms, can solve the problems of waste water pollution, complicated operation of machinery and equipment, difficult post-processing, etc., and achieve the effects of simple equipment structure, enhancement of body immunity, and reduction of breeding costs.

Active Publication Date: 2014-09-03
内蒙古科拓微生态科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When liquid fermentation is used, a large amount of waste water will be produced to pollute the environment, the post-treatment is difficult, and the operation of machinery and equipment is also relatively cumbersome.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A kind of probiotics prepared by utilizing Bacillus coagulans and Lactobacillus plantarum, its preparation method comprises the steps:

[0021] Preparation of seed liquid: Bacillus coagulans HM-09, Lactobacillus plantarum HM-10 and Lactobacillus casei HM-09 were inoculated into the medium containing liquid seeds from frozen glycerin tubes, and the Bacillus coagulans HM- 09 seed culture conditions: 37°C, 170 rpm shaker culture for 24 hours; Lactobacillus plantarum HM-10 and Lactobacillus casei HM-09 were cultured in an incubator at 37°C for 24 hours;

[0022] The seed medium is: 2% peptone, 2% glucose, added water to 100%, pH 7.0-7.2.

[0023] solid state fermentation

[0024] Fermentation culture conditions: Add the Bacillus coagulans HM-09 seed solution to the solid medium for mixing and ferment for 12 hours, then inoculate the Lactobacillus plantarum HM-10 seed solution and Lactobacillus casei HM-09 seed solution and continue to ferment for 24 hours, total fermentati...

Embodiment 2

[0030] A kind of probiotics prepared by utilizing Bacillus coagulans and Lactobacillus plantarum, its preparation method comprises the steps:

[0031] Preparation of seed solution: Bacillus coagulans HM-09 was cultured in an incubator at 37°C for 24 hours on a shaker at 170 rpm; Lactobacillus plantarum HM-10 and Lactobacillus casei HM-09 were cultured in an incubator at 37°C for 24 hours.

[0032] The seed medium is: peptone 2%, glucose 2%, add water to 100%, pH7.0-7.2;

[0033] Fermentation culture conditions: Add the Bacillus coagulans HM-09 seed solution to the solid medium, mix and ferment for 16 hours, then inoculate the seed solution of Lactobacillus plantarum HM-10 and Lactobacillus casei HM-09 and continue to ferment for 15 hours, the total fermentation time is 31 hours During the fermentation process, the fermentation temperature is controlled to be 37°C, the humidity is 80%, and the free amino nitrogen content is 486umol / g after the fermentation is completed;

[0034]...

Embodiment 3

[0039] A kind of probiotics prepared by utilizing Bacillus coagulans and Lactobacillus plantarum, its preparation method comprises the steps:

[0040] Preparation of seed solution: Bacillus coagulans HM-09 was cultured in an incubator at 37°C for 24 hours on a shaker at 170 rpm; Lactobacillus plantarum HM-10 and Lactobacillus casei HM-09 were cultured in an incubator at 37°C for 24 hours.

[0041] The seed culture medium of Bacillus coagulans HM-09, Lactobacillus plantarum HM-10 and Lactobacillus casei HM-09 is: peptone 2%, glucose 2%, add water to 100%, pH7.0-7.2;

[0042] Fermentation culture conditions: Add the Bacillus coagulans HM-09 seed liquid to the solid medium for mixing and ferment for 6 hours, then inoculate the seed liquid of Lactobacillus casei HM-09 and Lactobacillus plantarum HM-10 and continue to ferment for 36 hours, with a total fermentation time of 42 hours During the fermentation process, the fermentation temperature is controlled to be 37°C, the humidity ...

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PUM

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Abstract

The invention discloses probiotics prepared from bacillus coagulans and lactobacillus plantarum as well as a preparation method thereof and belongs to the technical field of microorganisms of feeding stuff. The probiotics is prepared by carrying out solid-state fermentation on matrix by using bacillus coagulans, lactobacillus plantarum and lactobacillus casei, wherein the matrix includes soybean meal, bran and corn flour. The fermentation method has the following advantages that the fermentation cost is reduced, the processing is convenient, the pollution is hardly caused, equipment is simple in structure and easy to operate; and therefore the fermentation method is a probiotics solid fermentation technology which can be widely applied to the field of micro-ecology; the probiotics disclosed by the invention is capable of degrading macromolecule proteins in the soybean meal, the bran and the corn flour into oligopeptides and completely decomposing anti-nutrients, antigen protein and the like in the protein, so that the protein quality and the feeding value of fermented products such as the soybean meal are improved; and the fermented products contain viable bacteria and are capable of effectively adjusting microbial florae of animal intestines, improving production performance and increasing the utilization rate of the feeding stuff.

Description

technical field [0001] The invention belongs to the technical field of feed microorganisms, and in particular relates to an applied technology for preparing probiotics by solid-state fermentation of Bacillus coagulans and Lactobacillus plantarum using soybean meal, bran and corn flour as substrates. Background technique [0002] Most of the existing probiotic products use probiotics to directly ferment with liquid milk and soy milk to obtain functional yogurt or soy milk, or directly collect probiotic cells after liquid fermentation to make probiotic powder, tablet or capsule. When liquid fermentation is used, a large amount of waste water will be produced to pollute the environment, the post-treatment is difficult, and the operation of machinery and equipment is also relatively cumbersome. Since probiotics can improve the micro-ecological balance of the host, improve the health level of the host and live bacterial preparations and metabolites of the healthy state, the rese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/245C12R1/25A23K10/18
Inventor 赵树平姚国强
Owner 内蒙古科拓微生态科技发展有限公司
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