Method for producing trichoderma solid spawn by taking amino acid hydrolytic solution and rice straw as raw materials
A technology of rice straw and solid strains, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of environmental pollution, irrational use of resources, etc., and achieve the effect of good quality and good nutrient conditions
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0040] 1. Observation of bacterial strains
[0041] The strain is Trichoderma guizhouense NJAU4742 (Trichoderma guizhouense), which has the following characteristics: Trichoderma guizhou is isolated from traditional Chinese medicine residue and composted, and belongs to the branch of Trichoderma harzianum in evolution; its hyphae can strongly parasitize Botrytis cinerea, Alternaria, Sclerotinia sclerotiorum, Rhizoctonia solani, Sclerotinia sclerotiorum and Fusarium oxysporum; Trichoderma guizhou has a unique light sensitivity, which increases the concentration of spores; in situ fluorescence studies show that Trichoderma guizhou can colonize soil and plant rhizosphere , promote plant growth, inhibit plant diseases, and increase crop yields.
[0042] 2. Culture of strains
[0043] 1) Inoculate the strain NJAU4742 into a solid medium PDA glass petri dish. The culture conditions are: 28°C, 7 days. Wash and scrape the green spores on the petri dish with 5 mL of sterile water, and...
Embodiment 2
[0046] 1. Preparation method of waste livestock and poultry amino acid hydrolyzate
[0047] (1) In a closed container, the sick and dead livestock and poultry animals are automatically crushed, and all solids and liquids are automatically transferred to a closed hydrolysis tank. After the initial acid concentration c (1 / 2H 2 SO 4 ) is 3-5mol L -1 , hydrolysis at 80-100°C and 1-2 atmospheric pressure for 2-5 hours;
[0048] (2) When the hydrolysis is completed and the solution in the hydrolysis tank is cooled to below 80°C, use a vacuum suction device that can enter and exit the feed port of the hydrolysis tank to float upright on the grease to separate the upper layer of grease;
[0049] (3) After the oil is separated, slowly add potassium hydroxide in the hydrolysis tank according to the mass ratio of the hydrolyzate to potassium hydroxide at 4:1, stir while adding, and adjust the pH value of the solution to 1-3; or press the hydrolyzate The mass ratio of lobster shells or...
Embodiment 3
[0064] Example 3 Guizhou Trichoderma NJAU4742 Solid Fermentation
[0065] After adding 10L of tap water into the large-capacity transfer box, add 285mL of waste livestock and poultry amino acid hydrolyzate to fully dissolve, then add 1.5Kg of crushed rice straw, stir well and soak overnight at room temperature (the straw can be softened and the straw can be treated with low pH conditions) Sterilize), adjust the pH of the stalk to 3.5 with solid KOH and a little amino acid stock solution after overnight, remove the raw materials that have adjusted the pH, adjust the water content to 75% and stir until fluffy, add 150g corn flour at the same time, fully stir Evenly get the fermentation substrate of Guizhou Trichoderma 4742, according to 20% (spore concentration 10 8 spore / mL, v / w) to inoculate Trichoderma Guizhou 4742 fermentation broth, spread the solid substrate in a shallow plate, the thickness of the straw is about 2cm-5cm, and place it in a dark room at 28°C-32°C for the se...
PUM
Property | Measurement | Unit |
---|---|---|
thickness | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com