382 results about "Industrial fermentation" patented technology

The present invention provides a recombinant microbial strain capable of stably producing a polyhydroxyalkanoic acid (PHA) at a high production rate in an industrial fermentation process. The present invention also relates to a recombinant microbial strain prepared by substituting an exogenous polyhydroxyalkanoic acid synthase gene for a polyhydroxyalkanoic acid synthase gene on the chromosome of the microorganism.

A method for producing the high-value ketocarotenoid astaxanthin by the green microalga Chlorella zofingiensis in dark-heterotrophic cultures shows excellent growth and high-yield astaxanthin production on glucose-supplemented media in the dark. The specific growth rate and astaxanthin yield can be as high as 0.031 h−1, and 10.3 mg l−1, respectively, which are the highest so far reported in heterotrophic algal cultures. The light-independent astaxanthin-producing ability of Chlorella zofingiensis can be employed for commercial production of astaxanthin using industrial fermenters.

The invention relates to a household wine brewing machine and a method for brewing wine by using the household wine brewing machine. The household wine brewing machine can be used for overcoming the defects that the existing industrial fermentation equipment is oversized and cannot achieve miniaturization and domestication. The household wine brewing machine is divided into four parts, namely a sealing cover, a fermentation tank, a control part and a power part, wherein the fermentation tank adopts a brand-new design. The household wine brewing machine not only can be used for producing grape wine, but also can be used for producing various fruit wines and beers, so that the integrated production of various wines is realized to provide more choices for consumers, the equipment cost is lowered and the product quality is guaranteed.

The invention discloses a high-protein straw feed, which comprises crop straw powder, actinomycetes, nocardia, lactobacillus acidophilusis and bacillus subtilis, the weight ratio of the strains is 0.8-1.2:1.6-2.4:0.8-1.2:1.8-1.2, and the weight ratio of the total strain amount to the crop straw powder is 1:1,000-1,200. The preparation method comprises the following steps of: respectively screening, separating, purifying, rejuvenating and culturing various strains, transplanting the strains into an industrial fermentation tank for fermentation, mixing in a ratio, and adsorbing by using an adsorptive support; and inoculating the obtained composite viable bacteria powder into crop straw powder or a mixture of the crop straw powder, staking and fermenting at the temperature of between 60 and 70 DEG C, uniformly turning once every two days, and obtaining the high-protein straw feed after 30 to 35 days. The high-protein straw feed has the advantages of improving protein content, optimizing protein structure, improving the absorption and nutrient conversion rate of the straw feed, and improving the immunity of livestock.

A method for preparing industrial fermentation pickled vegetable relates to a method for preparing fermentation pickled vegetable, solving the problems that pickled vegetable produced by the current large-scale factory is still prepared by traditional technique depending on natural fermentation and the cabbage in the fermentation jar can be easily damaged by other bacteria and be easily rotten and goes bad. The method comprises the following steps: 1, palletizing cabbages; 2, adding pickled vegetable fermentation engineering bacteria; 3, fully injecting brine to the fermentation jar; 4, carrying out fermentation to obtain the product of pickled vegetable. The method uses controllable industrial technique to ferment the pickled vegetable; the added components are known and controllable; thus, the method avoids the pollution of the cabbage by other bacteria, rot and metamorphism.

The present invention discloses a fermenting production process of Hirsutella hepiali Chen & Shen for industrial purpose. It contains steps: a. Isolating new stain from original source; b. identifying whether the stain can grow stroma or not; c. culturing the strain in solid medium for rejuvenescence purpose; d. Secondly culturing the strain in liquid culture medium; e. fermenting. Said method provides a fermenting production process, which can continually identify whether the anamorphic fungi related to Chinese Cordyceps sinensis change or not, whether it retains the property of original strain or not. It also can be modified continually. According to these processes, the quality of the obtained product will be stable and the property will be retained stable for quite a long time. Therefore this method overcome the problems exist in the art that change of the strain, instability of quality of the product and so on.

The invention discloses a two-step hydrolysis method for preparing reducing sugars with cellulose, comprising the following steps: step (1) of dissolving the cellulose into a solvent to obtain a cellulose solution; in the cellulose solution, enabling the cellulose to contact water by the action of a first catalyst to perform a first-step hydrolysis and obtain hydrolysate; adding a precipitator into the hydrolysate, and separating after precipitating to obtain precipitate; step (2) of adding a second catalyst in the precipitate obtained in the step (1) by utilizing water as a medium to perform a second-step hydrolysis and obtain the reducing sugars, wherein productivity of the reducing sugars reaches 40-85%. The invention further discloses application of the two-step hydrolysis method for preparing reducing sugars with cellulose in fermentation and preparation for compounds such as ethanol, lactic acid and so on in the carbohydrate industry. Cellulose solvent, catalyst and precipitator utilized by the invention can effectively separated and used repeatedly; hydrolysis condition is relatively mild; dosage of acid catalysts is less; and the method is featured with simple operation, low energy consumption, fast reaction and high productivity.

The invention discloses a method for processing agricultural raw materials and producing a culture medium for industrial fermentation by utilizing a steam explosion technology aiming at the problems that the raw material utilization rate is low, the crushing power consumption is high, the amount of hydrolysis acids and alkalis is large and the like when the agricultural raw materials are used for an industrial fermentation culture medium. By utilizing the steam explosion technology, starch, proteins, lipids, celluloses, hemicelluloses, lignins and other components in the agricultural raw materials are degraded or modified, macromolecular substances are degraded into small molecular substances, and fermentation promoted substances are generated. According to the method, the energy consumption in the process of further processing the agricultural raw materials is reduced, the product performance of the agricultural raw materials serving as the industrial fermentation culture medium is improved, the raw material utilization rate in the fermentation process is improved, and growth and metabolism of microbes and the conversion rate of the fermented product are promoted by a fermentation accelerant generated in the steam explosion treatment process. The agricultural raw materials subjected to the steam explosion treatment can serve as a culture medium for solid state fermentation and also can be used for a culture medium for liquid state fermentation.

The invention discloses bacillus subtilis and use thereof in bio-control preparation for preventing and controlling powdery mildew and belongs to the field of microbial pesticides. The strain is (bacillus subtilis)FBR1 with a collection number of CGMCC No.4915. The industrial fermentation culture medium for the bacillus subtilis is prepared from 0.8 percent of corn flour, 1.5 percent of soybean flour, 0.1 percent of yeast powder, 0.5 percent of peptone, 0.8 percent of monopotassium phosphate, 0.15 percent of calcium carbonate, 0.02 percent of magnesium sulfate and 0.001 percent of defoamer, and by the steps of adjusting the pH value to 7.0, fixing volume by using water, and fermenting for 24 to 36 hours under conditions of a fermentation temperature of 28 to 35 DEG C, a ventilation volumeof 0.2 to 0.4 v / v / min and a stirring speed of 100 to 150r / min. The invention also discloses a bio-control preparation which contains bacillus subtilis and can be used for preventing and controlling powdery mildew in economic crops such as melons, vegetables, tobacco and strawberry in place of spray chemical medicines without polluting the environment.