The invention relates to the technical field of healthcare food, in particular to healthcare sesame oil with beautifying and blood nourishing efficacy and a preparation method thereof. The healthcare sesame oil is made of raw materials comprising, by weight, 1200-1400 parts of black sesame, 6-12 parts of angelica sinensis, 20-25 parts of wolfberries, 12-15 parts of ligusticum wallichii, 20-25 parts of glossy privet fruit, 15-18 parts of chamomile, 12-15 parts of astragalus, 16-20 parts of lilium brownii, 10-15 parts of polygonum multiflorum, 8-10 parts of eclipta alba and 12-15 parts of roses. The sesame oil is rich in the raw materials and simple and efficient in producing process, a plurality of traditional Chinese medicine components such as the angelica sinensis, the wolfberries and the ligusticum wallichii are combined, efficacy of liver and kidney nourishing, five-organ moistening, beautifying and blood nourishing and the like is achieved, and high food healthcare value is achieved. The sesame oil is obtained by adopting a carbon dioxide subcritical extraction technology, the production process is environment friendly and efficient, extraction temperature is low, bioactive components in the raw materials cannot be destroyed, and a high promotion application value is achieved.