Winter jujube preservative and preparation method and application thereof

A technology of antistaling agent and winter jujube, which is applied in application, fruit and vegetable preservation, dairy products, etc., can solve the problems of reducing the stability of natamycin and increasing the solubility of natamycin, so as to enhance the activity of inhibiting mold and make it moist. Wet suspending effect, high food safety effect

Inactive Publication Date: 2019-05-17
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some people in foreign countries increase the solubility of natamycin by adjusting the pH value of the solution (United States Patent: US7816332B2), but when the pH is lower than 3 or higher than 9, the stability of natamycin will be reduced

Method used

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  • Winter jujube preservative and preparation method and application thereof
  • Winter jujube preservative and preparation method and application thereof
  • Winter jujube preservative and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Dissolve natamycin in sterilized water, dilute it with PDA medium into a concentration gradient: 5mg / L, 10mg / L, 20mg / L, 40mg / L, 80mg / L, and then place it in a 9cm petri dish , inoculate the Alternaria bacterium block (0.5cm) in the center of the petri dish, observe for 7 days, use the sterilized water group as a contrast, calculate the inhibition rate according to the following formula.

[0028] Dissolve chitosan in sterilized water, dilute it with PDA medium into a concentration gradient: 200mg / L, 400mg / L, 800mg / L, 1600mg / L, 3200mg / L, and then place it in a 9cm petri dish. Inoculate the Alternaria bacterium block (5mm) in the center of the petri dish, observe for 7 days, use the sterilized water group as a control, and calculate the inhibition rate according to the following formula.

[0029] Inhibition rate=(mycelia growth length of control group-mycelia growth length of treatment group) / (mycelia growth length of control group-5mm)×100%.

[0030] Then apply GraphPadP...

Embodiment 2

[0041] Dissolve natamycin in sterilized water, dilute it with PDA medium into a concentration gradient: 5mg / L, 10mg / L, 20mg / L, 40mg / L, 80mg / L, and then place it in a 9cm petri dish , inoculate the brown rot bacteria block (5mm) in the center of the petri dish, observe for 7 days, use the sterilized water group as a contrast, calculate the inhibition rate according to the following formula.

[0042] Dissolve chitosan in sterilized water, dilute it with PDA medium into a concentration gradient: 200mg / L, 400mg / L, 800mg / L, 1600mg / L, 3200mg / L, and then place it in a 9cm petri dish. Inoculate the brown rot bacteria block (5mm) in the center of the petri dish, observe for 7 days, use the sterilized water group as a control, and calculate the inhibition rate according to the following formula.

[0043] Inhibition rate=(control group mycelial growth length-treatment group mycelial growth length) / (control group mycelial growth length-5mm) 100%

[0044] Then apply GraphPadPrism5 softwar...

Embodiment 3

[0054] Natamycin and chitosan are mixed according to a mass ratio of 1:100, and added to sterilized water to prepare a suspension mother liquor mixed with natamycin and chitosan, thereby obtaining the winter jujube preservative. Apply this winter jujube antistaling agent to the post-harvest preservation of winter jujube. The specific operation is: dilute the mother liquor to a concentration range of 500-1500mg / L, soak the winter jujube in this preservative (500mg / L in this experiment) for 5 minutes, After natural drying, it is packed in a polyethylene fresh-keeping bag, and the observation results after storage at 5°C for 45 days are as follows: figure 1 and figure 2 As shown, the control is to replace the winter jujube preservative with sterilized water. from figure 1 and figure 2 It can be seen that the winter jujube antistaling agent of the present invention can better prevent and cure the winter jujube rot and mature senility, reduce the rot rate by 20-40%, and signif...

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Abstract

The invention discloses a winter jujube preservative and a preparation method and application thereof. The winter jujube preservative includes natamycin, chitosan or chitosan derivatives and solvents.According to experiments, the chitosan or the derivatives have a very good wetting and suspending effect on natamycin, and the chitosan has a synergistic effect on the natamycin, that is, the natamycin can significantly enhance the inhibition of the mold activity after mixed with the chitosan, and has a good preservation effect on winter jujubes. Therefore, the preservative can be used for winterjujube preservation. The product is prepared from food additives, has high food safety and is suitable for production and storage of dairy products, meat, beverages, fruit and other food.

Description

technical field [0001] The invention belongs to the field of fruit fresh-keeping, and in particular relates to a winter jujube fresh-keeping agent and a preparation method and application thereof. Background technique [0002] Winter jujube is one of the high-grade fruits in my country, and its vitamin C content is extremely high; however, winter jujube is not resistant to storage, and even if it is stored at a low temperature of 0 degrees Celsius, it is prone to diseases. The main postharvest diseases of winter jujube are Alternaria alternata and brown rot Bacteria (Monilinia fracticola). [0003] Natamycin (Natamycin) is mainly a polyolefin macrolide antifungal compound produced by Streptomyces fermentation. It not only has a strong inhibitory effect on the growth of mold and yeast, but also inhibits the growth of mycotoxins. It is safe for humans and animals, and has no carcinogenic, teratogenic, or mutagenic effects. Therefore, natamycin is widely used in food preservat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/154A23B7/155A23C3/08A23B4/20A23B4/22A23L2/44
Inventor 龚亮谢加勇蒋跃明张丹丹
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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