24H+ Antibacterial liquid is made from, by weight, 5 parts of lightyellow sophora root liquid, 3 parts of sessile stemona root liquid, 8 parts of amur corktree bark liquid, 8.5 parts of aloe liquid, 8.5 parts of common cnidium fruit liquid, 0.5 part of chlorhexidine acetate, and 70 parts of purified water. A preparation method of the 24H+ antibacterial liquid comprises: first, taking lightyellow sophora root extract, sessile stemona root extract, amur corktree bark extract, aloe extract, common cnidium fruit extract, and chlorhexidine acetate extract; second, adding the extracts into purifiedwater, mixing well, using a stirring rod to continuously mix, allowing standing, and filling. The preparation method herein is simple; the 24H+ antibacterial liquid has good antibacterial effect uponthe fungi, such as Staphylococcus aureus, Escherichia coli and Candida albicans, and has the advantages of pudendum cleaning and moistening, freshness, mildness and coolness, and good safety and convenience.