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Bacillus subtilis strain and application thereof

A technology of Bacillus subtilis and ZJU007, which is applied in the field of bioengineering, can solve problems such as no treatment methods, and achieve strong inhibitory effect, good antibacterial effect, and strong antibacterial effect

Inactive Publication Date: 2011-02-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, topical therapy has been able to cure most patients with superficial mycosis, but there are still few drugs that can treat deep mycosis, and some diseases still have no effective treatment

Method used

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  • Bacillus subtilis strain and application thereof
  • Bacillus subtilis strain and application thereof
  • Bacillus subtilis strain and application thereof

Examples

Experimental program
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Effect test

Embodiment Construction

[0027] culture medium

[0028] (1) LB slant medium (%): yeast powder 0.5, peptone 1.0, NaCl 0.5, agar 2.0, adjust pH to 7.4-7.6.

[0029] (2) Seed medium (%): glucose 1.5, yeast powder 1.0, K 2 HPO 4 0.1, MgSO 4 0.05, FeSO 4 0.001, add CaCO after adjusting pH7.0 3 0.04.

[0030] (3) Fermentation medium (%): soybean powder 0.6, soluble starch 3.0, yeast powder 1.0, K 2 HPO 4 0.1, NaCl 0.2, MgSO 4 0.005, FeSO 4 0.002, pH 6.5.

[0031] (4) Candida albicans medium (%): glucose 2.0, peptone 1.0, agar 2.0.

[0032] Strain isolation and purification

[0033] Soil samples were cross-sampled at five points in the area demarcated in the East China Sea of ​​Zhejiang Province. Take 10g of soil samples at each point and put them into conical flasks. bottle, placed on a shaker and vibrated for 30 minutes, so that the bacteria in the soil sample were fully dispersed in the water, that is, 10 -1 bacteria liquid. After the inoculation loop was flame sterilized and cooled, dip a r...

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Abstract

The invention discloses a Bacillus subtilis strain and application thereof. The strain is named as Bacillus subtilis ZJU007 and preserved in the China general microbiological culture collection center (CGMCC) of Chinese Academy of Sciences on yard No.1 on Beichen West Road, in Chaoyang district in Beijing on September 3rd, 2010, and has the preservation number of CGMCC No.4140. A bactericidal substance generated by the strain of the invention has very strong inhibition effect on Candida albicans and has better bacteriostasis effect compared with common fungicidin. The bactericidal substance not only has very strong bacteriostasis effect on the Candida albicans and other yeast like fungi, and the like, but also has excellent effect on Aspergillus niger and other filamentous fungi, and meanwhile, has better bacteriostasis effect on escherichia coli and other bacteria.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a bacillus subtilis strain and an application thereof. Background technique [0002] In recent years, with the improvement of modern medical treatment, the survival rate of patients with cancer, AIDS, organ and bone marrow transplantation has increased greatly. Chemotherapy, venous catheterization, hemodialysis and other interventional treatments lead to decreased immunity, and are easily invaded by conditionally pathogenic fungi that are not originally pathogenic, causing deep mycoses (Systemic fungal infection). In recent years, this serious life-threatening fungal infection has shown a rapid growth trend, and the number of patients has continued to increase. [0003] Clinically, with the increase of fungal infection rate, the utilization rate of antifungal drugs has increased, and the drug-resistant strains have also increased, which brings certain difficulties to clin...

Claims

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Application Information

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IPC IPC(8): C12P17/08C12N1/20C12R1/125
Inventor 吴绵斌贺娟张书衍陈华
Owner ZHEJIANG UNIV
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