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62 results about "China sea" patented technology

Satellite image of the South China Sea

Sea yeast for diseases biological control of postharvest fruits and vegetables, and preparation and use thereof

The invention discloses a marine yeast for biologically preventing and treating postharvest diseases of fruits and vegetables, a method for preparing the same and application thereof. A marine yeast strain Rhodosporidium p aludigenum Fell & Tallman is screened out from the seawater of the coastal waters of the East China Sea. The colony of the marine yeast is pink, has a smooth surface, and grows in a culture medium added with 1 to 2 percent NaCl. The use of a cell suspension of the yeast with certain concentration can effectively inhibit various postharvest main mycoses such as alternaria, botrytis cinerea, penicilllum expansum, penicillium digitatum, and pestalotia. The marine yeast strain Rhodosporidium p aludigenum Fell & Tallman is used for biologically preventing and controlling the postharvest diseases of fruits and vegetables for the first time both at home and abroad. The strain has various functions of preventing decay, controlling oxidative stain, controlling softening flavour reversion and the like, and has the advantages of convenient use, no generation of antibiotic, no environment pollution, security, innocuity, and broad market prospect. At the same time, the strain is conducive to the export for earning foreign currency of China's high-quality fruit and vegetable products, and has significant economic and social benefits.
Owner:ZHEJIANG UNIV

Method for manufacturing collagen from black sea cucumbers from East China Sea

ActiveCN102251006AMolecular mass size controllableFermentationFiltrationHydrolysis
The invention relates to a method for manufacturing collagen from black sea cucumbers from East China Sea, which comprises the following steps: (1) adding Alcalase hydrolysis protease in an amount which is 0.01 to 10 percent based on the weight of black sea cucumbers from East China Sea, protamex composite protease in an amount which is 0.01 to 10 percent based on the weight of black sea cucumbers from East China Sea and flavourzyme in an amount which is 0.01 to 10 percent based on the weight of black sea cucumbers from East China Sea into the black sea cucumbers from East China Sea which are subjected to foul-removing and cleaning treatment, heating the materials to 45 to 47 DEG C, and hydrolyzing at a constant temperature of 45 to 47 DEG C with stirring for 4 hours; and (2) after enzyme hydrolysis is finished, performing fine filtration by a membrane filter, concentrating in a vacuum concentrator, keeping the temperature of the materials in the vacuum concentrator between 55 and 58 DEG C by controlling the heating steam valve of the vacuum concentrator, and concentrating to obtain pasty or powder collagen product from black sea cucumbers from East China Sea. The method has the advantages that: a technical route of combination of three enzymes and hydrolysis degree intermediate detection is adopted, so the relative molecular weight of the product of the enzyme hydrolysis of the black sea cucumbers from East China Sea can be controlled to meet the requirement on the relative molecular weight of sea cucumber collagen products; and the product of the hydrolysis product is free from bitter taste and fishy smell.
Owner:宁波超星海洋生物制品有限公司

Breeding and propagation method for Atlantic deep sea scallops

The invention relates to an aquaculture method of deep sea scallops, in particular to a breeding and propagation method for Atlantic deep sea scallops. The method comprises the steps that introduction, artificial breeding and aquaculture of the deep sea scallops are carried out, parent scallops are mature after 45-50 days maturity promoting and lay eggs, and after most larvae grow into D-shaped larvae, a bolting cloth net cage is used for selecting optimal larvae to a new pond for cultivation; the growth rate of the cultured larvae is far higher than that of the larvae cultured in a nursery ground in Quebec of Canada at the same time, eyespots are generated on most larvae 16-17 days after the larvae are fertilized, adhering substances are arranged at the 18th day, after the adhering substances are arranged for 10 days, the adhering substances are taken out and hung in natural sea ears for larva protection; the deep sea scallops meet commercial specification when the cultivated average specification reaches 8 centimeters, and the deep sea scallops achieve sexual maturity. According to the method, the Atlantic deep sea scallops are introduced into China sea areas for the first time, the scallop culture species are expanded, and construction of modern ocean ranches is promoted.
Owner:DALIAN CHANGHAI ZHENLU AQUATIC PROD

Hybrid breeding method for stichopus japonicus

The invention relates to a hybrid breeding method for stichopus japonicus, comprising the following steps: selecting stichopus japonicus in China sea areas and in Korea sea areas as parental stichopus japonicus, respectively controlling breeding water temperature, times and time of feeding of the parental stichopus japonicus in an artificial culture, and adding yolk and pachyrhizus powder of different amounts for stimulating gonadogenesis in breeding bait containing alga powder, compound feed and holothurian vitamin to enable synchronous maturity of gonads of the parental stichopus japonicus; stimulating the parental stichopus japonicus by a shade-drying running water stimulating method, a temperature differential method and an artificial induced spawning method combined with a method of using spermia to stimulate ovum, to induce the oviposition and the spermiation of the parental stichopus japonicus; and respectively collecting the ovum and the spermia of the parental stichopus japonicus; carrying out reciprocal hybridizations to produce oosperm; and breeding according to a conventional method. The stichopus japonicus sprout cultured by the method has high survival rate, fast growth rate, good stress resistance, and strong ability of resisting mechanical damage. The process is easy and convenient, and can be operated easily, effectively shortens the period of breeding the stichopus japonicus and saves the breeding cost.
Owner:SHANDONG HOMEY AQUATIC DEV

Culturing method of sea source bacillus subtilis insect killing activity product and its application

A process for culturing the Bacillus subtilis coming from sea to obtain the insecticiding product for more agricultural pests includes bioactive screening to the marine microbes in Chinese sea to obtain a high-activity Bacillus subtilis strain, and culturing it in fermenting culture medium to obtain said active insecticiding product. Its advantages are high killing activity to agricultural pests, broad spectrum, and no environmental pollution.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES

Ecological risk assessment method of polycyclic aromatic hydrocarbon compound and mixture thereof in marine water environment

InactiveCN103853931ASpecial data processing applicationsMaterial analysisPredicted no-effect concentrationFlora
The invention discloses an ecological risk assessment method of a polycyclic aromatic hydrocarbon compound and a mixture thereof in a marine water environment. The assessment method comprises the following steps of determining the type and concentration of the polycyclic aromatic hydrocarbon in the marine water environment; screening the representative biological species having the flora characteristics of the creatures of the China sea area; acquiring the chronic toxicity data of polycyclic aromatic hydrocarbon in the water body of a target sea area; using a statistical extrapolation method to calculate the predicted no effect concentration of polycyclic aromatic hydrocarbon; using a joint probability curve method to calculate the level of the ecological risk of single polycyclic aromatic hydrocarbon in the water body of the target sea area; using a toxicity equivalent factor method to calculate the joint ecological risk of the polycyclic aromatic hydrocarbon mixture in the water body of the target sea area; identifying the risk of the monomer and the mixture of the polycyclic aromatic hydrocarbon according to the above results. The ecological risk assessment method of the polycyclic aromatic hydrocarbon compound and the mixture thereof in the marine water environment provided by the invention realizes the ecological risk assessment of the polycyclic aromatic hydrocarbon monomer and the mixture thereof in the China marine water environment by using the toxicity equivalent and the probability risk assessment method based on the species sensitivity distribution theory, and provides a decision basis for the marine ecological risk management of the China organic pollutants.
Owner:NATIONAL MARINE ENVIRONMENTAL MONITORING CENTRE

Preparation technology of mytilus crassitesta lischlk polysaccharide compound with anti-tumour activity

The invention relates to the technical field of medicines and provides a new method for separating a polysaccharide compound from mytilus crassitesta lischlk produced in the East China sea and purifying the polysaccharide compound. The invention also provides application of the polysaccharide compound in preparation of an anti-tumour drug.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY

Method for extracting and purifying pepsin-soluble collagens of black sea cucumber in East China Sea

The invention provides a method for extracting and purifying pepsin-soluble collagens of black sea cucumbers in East China Sea. The method comprises the following steps: soaking a clean, massive black sea cucumber sample in East China Sea with an EDTA (Ethylene Diamine Tetraacetic Acid) solution; performing homogenized homogeneity; performing extraction in sequence by using a Tris-HCl solution, a NaOH solution and a pepsase acetic acid solution; and performing salting out and dialysis to obtain the pepsin-soluble collagens. According to the invention, the EDTA chelating agent is used to remove chemical element with high pollution concentration from sea cucumber tissues, and the removal efficiency can reach more than 90%, a crosslinking effect in chemical bonds and among the chemical bonds of a collagen albumen product can be effectively removed after pepsase is used for performing hydrolysis on collagen fibers, so that maximally extracting the solvation collagen albumen can be realized; after the purification steps of sodium chloride salting out and dialysis, and the like are carried out, and the purity of the obtained collagen albumen product by extraction can reach more than 90%, the yield can reach 56.99-61.52% (dry weight), which is 97.1-102.5% of the total collagen albumen content of the black sea cucumber in East China Sea, so that the extraction of collagen albumen content with high productivity of the black sea cucumber in East China Sea can be realized.
Owner:ZHEJIANG UNIV OF TECH

Laurane type sesquiterpene compound or derivative thereof and preparation method and application thereof

InactiveCN101628855ASignificant pancrelipase inhibitionOrganic active ingredientsOrganic chemistryFatty liverHydrogen
The invention relates to a laurane type sesquiterpene compound or a derivative thereof extracted and separated from a sea plant Laurencia okamurai in the East China Sea, and a preparation method and application thereof. The structural formula of the laurane type sesquiterpene compound or the derivative thereof is shown as above: when R1, R2 and R3 are hydrogen at the same time, the sesquiterpene compound or the derivative thereof is laurinferol, and when R4 is hydrogen, the sesquiterpene compound or the derivative thereof is aplysin. In vitro tests show that the sesquiterpene compound or the derivative thereof has obvious pancreatic lipase inhibition activity and is expected to be applied to preparing medicaments for preventing or treating decompensation diseases including adiposis, diabetes, hyperlipoidemia, fatty liver and the like. The sesquiterpene compound or the derivative thereof can also provide a lead compound for developing the new medicaments for preventing and treating the decompensation diseases, and is significant for developing and utilizing marine biological resources of China.
Owner:SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI

Solid fermentation method for producing salt-tolerant cellulase by using oceanic Aspergillus niger

The invention discloses a solid fermentation method for producing salt-tolerant cellulase by using an oceanic Aspergillus niger, comprising the following steps of: (1) screening an oceanic Aspergillus niger from soil in the depth of 10-20m approaching to the East China Sea; (2) vaccinating the oceanic Aspergillus niger on a PDA culture medium for culturing to obtain an inclined-plane spore, and preserving the inclined-plane spore at 4 DEG C; (3) vaccinating the inclined-plane spore on a PDA-1 culture medium for culturing to obtain an activated spore; (4) vaccinating the activated spore on an eggplant bottle inclined plane for culturing for 74h at 42 DEG C to obtain an eggplant bottle inclined-plane spore; and (5) propagating the obtained eggplant bottle inclined-plane spore, diluting the eggplant bottle inclined-plane spore to 108 spores / ml by using sterilized artificial seawater, vaccinating to a solid fermentation enzyme-producing culture medium, and fermenting for 8-12 days to obtain the cellulase. The solid fermentation method has the characteristics of coarse culture medium, wide source, low price and the like, the produced cellulase can degrade cellulose under the condition of a higher salt content, and the enzyme is abnormally stable under high-salt environment, therefore, the produced cellulase lays the foundation for the subsequent application of hydrolysate.
Owner:ZHEJIANG UNIV

Equipment for extracting polypeptides in sea cucumbers in East China Sea

The invention relates to the technical field of polypeptide extraction accessory devices, and in particular relates to equipment for extracting polypeptides in sea cucumbers in the East China Sea. Theequipment comprises a hollow stirring kettle, a first threaded rod and a second threaded rod; a first bearing is arranged in the central area of the top of the stirring kettle; the inner ring of thefirst bearing is connected with a first stirring shaft; multiple first stirring blades are arranged on the outer side wall of the first stirring shaft; the interior of the first stirring shaft is hollow; a mechanical seal is arranged at the bottom in the first stirring shaft; a second bearing is arranged on the top in the first stirring shaft; a second stirring shaft is arranged on the inner ringof the second bearing; the second stirring shaft is rotatably connected with the mechanical seal; multiple second stirring blades (34) are arranged in the lower half area of the second stirring shaft;and a first bevel gear is arranged on the first stirring shaft. The equipment in the invention is simple in structure; the mixing effect and the mixing speed of a freeze-drying protective agent and semi-finished polypeptide concentrated solution are improved; the polypeptide concentrated solution and the freeze-drying protective agent on the inner wall are convenient to clean; and the product quality is improved.
Owner:WENZHOU VOCATIONAL COLLEGE OF SCI & TECH

Device for simulating whole cataclysm process of seabed soft soil under combined effects of typhoon waves and shallow buried high pressure gas

ActiveCN107966267AWave stabilityWave period can be controlledHydrodynamic testingEarth material testingInstabilityTorque motor
The invention discloses a device for simulating a whole cataclysm process of seabed soft soil under combined effects of upper-surface typhoon waves and pushing of a shallow buried high pressure gas, which can reproduce a weakening and pneumatic inrushing cataclysm whole process of the seabed soft soil under combined effects of the upper-surface typhoon waves and the pushing of the shallow buried high pressure gas. The device mainly comprises an outer model box, a soil model box, a perforated separator, a micro jack, a pneumatic cabin, a pneumatic servo motor, a shaft lever, a torque motor, a sliding guide rail and an impeller. A key physical simulation means is provided for revealing the seabed instability and cataclysm mechanism of three major waters (the Yellow Sea, the East China Sea and the South China Sea) with typhoon waves bursting and with high-pressure biogenic gas-containing seabeds distributed widely.
Owner:ZHEJIANG UNIV

Manufacturing method for sea cucumber essence pure powder

InactiveCN105851926AMolecular mass size controllableSuitable for the requirements of relative molecular massFood shapingBlack seaChina sea
The invention relates to a manufacturing method for sea cucumber essence pure powder. The method comprises the following steps: first, grinding East China Sea black sea cucumbers which are subjected to decontaminating and cleaning treatment into pulp by using a stainless steel grinder; adding the pulp into a stainless steel enzymolysis tank through a 40-mesh screen; adding tap water with the weight being 1.8 to 2.0 times weight of the East China Sea black sea cucumbers; performing heat-insulating heating at 95 DEG C for half an hour; closing steam; starting an enzymolysis tank stirring machine, and enabling the enzymolysis tank stirring machine to rotate by 30 rotations per minute; turning on enzymolysis tank jacket cooling water to decrease the temperature of materials to 45 DEG C; adding Alcalase proteolytic enzyme with the weight being 0.1 to 0.5 percent of the weight of the East China Sea black sea cucumbers, Protamex compound protease with the weight being 0.1 to 0.5 percent of the weight of the East China Sea black sea cucumbers, and Flavourzyme with the weight being 0.1 to 0.5 percent of the weight of the East China Sea black sea cucumbers into the materials; starting the enzymolysis tank stirring machine, and performing heat-insulating hydrolysis for four hours at 45 to 47 DEG C; filtering and concentrating the materials after enzyme hydrolysis is ended, wherein the temperature of the materials is controlled to be 55 to 58 DEG C, and concentrating is not ended until the solid content is 25 to 30 percent; performing spray-drying by using a centrifugal spray-drying machine, thus obtaining a finished product, namely the sea cucumber essence pure powder, wherein the temperature at the inlet of the spray-drying machine is controlled to be 210 to 215 DEG C, and the temperature at the outlet of the spray-drying machine is controlled to be 95 to 96 DEG C.
Owner:NINGBO HAIBOSHI TEYI FOOD TECH CO LTD

Hybrid seedlings-cultivating method between Chinese stichopus japonicus and Japan black stichopus japonicus

The application relates to a hybrid seedlings-cultivating method of the stichopus japonicus. The method comprises the steps of: firstly taking the stichopus japonicus in the China sea area and the black stichopus japonicus in the Japan northern sea area as the bred stichopus japonicus, and synchronously fertilizing the bred stichopus japonicus of the China stichopus japonicus and the bred stichopus japonicus of the Japan stichopus japonicus by controlling the cultivating water temperature and the bait feeding quantity of the bred stichopus japonicus in the process of artificial fertilizing and cultivating; artificially spawning and discharging sperm under the oxytocic condition of drying in the shade and warming, and respectively collecting the ovum from the Chinese stichopus japonicus and the sperm from the Japan black stichopus japonicus or the sperm from the Chinese stichopus japonicus and the ovum from the Japan black stichopus japonicus for the aritificial exomixis; and performing the incubation and the seedlings cultivation to the oosperm which is generated by hybridizing the two different geographical colonies which are reproductively isolated from each other according to the normal mode for cultivating the seedlings of the stichopus japonicus so as to cultivate the hybrid stichopus japonicus seedlings.
Owner:SHANDONG HAIYIBAO AQUATIC PROD

Feeding device and method for silvery pomfret in East China Sea

ActiveCN112154957AIncrease profitAvoid the problem of polluting the breeding environmentClimate change adaptationPisciculture and aquariaChina seaEngineering
The invention discloses a feeding device and method for silvery pomfret in the East China Sea, and aims to overcome the defects that in the silvery pomfret breeding process, the utilization rate of baits is low, and the baits sink to the bottom of a pond for a long time to pollute the breeding environment. The feeding device comprises a feeding barrel, a plurality of bait outlet nozzles are arranged at the lower portion of the feeding barrel, one-way cover pieces are installed at the opening ends of the bait outlet nozzles, a piston assembly is installed in the feeding barrel, a bait storage barrel, a lifting base and a driving assembly are installed on the upper portion of the feeding barrel, the lifting base is connected with the piston assembly, a material squeezing plug is installed inthe bait storage barrel, and a piston rod is connected betwene the material squeezing plug and the lifting base; a material extruding cavity is formed between the position, below the piston assembly,in the feeding barrel and the bottom of the feeding barrel, and a feeding pipe is connected between the piston assembly and a discharging hole; the lifting base moves upwards, the material squeezingplug pushes the baits to enable the baits to be injected into the material squeezing chamber through the feeding pipe, and meanwhile the lifting base drives the piston assembly to move upwards to enable the material squeezing chamber to be enlarged; and the lifting base moves downwards to drive the piston assembly to move downwards to extrude the baits in the material squeezing chamber out throughthe bait outlet nozzles.
Owner:MARINE FISHERIES RES INST OF ZHEJIANG
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