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Preparation technology of mytilus crassitesta lischlk polysaccharide compound with anti-tumour activity

A technology of thick-shelled mussels and anti-tumor drugs, applied in the field of medicine, can solve the problems of low polysaccharide yield, lipid interference and the like

Inactive Publication Date: 2015-04-29
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a new method for preparing polysaccharides contained in East my country Sea mussels in order to address the shortcomings of existing polysaccharide extraction methods, such as lipid interference and low yield of final polysaccharides; the first method of the present invention The second purpose is to provide the mussel thick shell polysaccharide compound prepared according to the above method; the third purpose of the present invention is to provide the application of the polysaccharide compound in the preparation of antitumor drugs

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0019] Example 1: Separation and purification of polysaccharides from thick-shelled mussels

[0020] Thick-shelled mussels were provided by Xiangyuan Aquatic Products Co., Ltd., Shengsi County, Zhejiang Province, and collected from the mussel breeding waters of Shengsi County, Zhejiang Province.

[0021] Take 700g of fresh mussel mollusc tissue, homogenize it with a tissue grinder, add 1L of 1% methanol intermittently, stir and soak for 24h to remove fat, and centrifuge at 5000rpm×10min to collect the precipitate. Add 5L of water, stir, boil for 5h, filter with suction, add water to the residue repeatedly, boil for 3h, filter with suction, and combine the two filtrates. The filtrate was concentrated by rotary evaporation at 50°C under reduced pressure. Decolorize with 0.5% activated carbon, and centrifuge at 5000rpm×10min to remove the precipitate. Add ethanol to a final concentration of 75%, and let stand at 4°C for alcohol precipitation overnight. The precipitate was coll...

Embodiment 2

[0023] Embodiment 2: the research of the antitumor activity of the compound of the present invention

[0024] 1. In vitro tumor cell proliferation inhibition experiment

[0025] First, a variety of tumor cell lines were screened to obtain tumor cells sensitive to mussel polysaccharides. Human ovarian cancer HO-8910, human breast cancer MCF-7, leukemia cells K562, leukemia cells HL-60, human liver cancer SMMC-7721, HepG-2, human cervical cancer Hela cells, human pancreatic cancer cells SW1990 and human gastric cancer Cells AGS, MKN-28, MKN-45, etc. were used as research objects, and tumor cells in the logarithmic growth phase were digested with 0.25% trypsin, and the cell concentration was adjusted to 2×10 5 / ml, seeded in 96-well plate, 6 replicate wells per group, 90 μl per well, 37°C, 5% CO 2 Adhere to the wall for 24 hours under the same conditions, add 10 μl of polysaccharide solutions with different concentrations, so that the final concentrations are 500 μg / ml, 100 μg / ...

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Abstract

The invention relates to the technical field of medicines and provides a new method for separating a polysaccharide compound from mytilus crassitesta lischlk produced in the East China sea and purifying the polysaccharide compound. The invention also provides application of the polysaccharide compound in preparation of an anti-tumour drug.

Description

technical field [0001] The present invention relates to the technical field of medicine, in particular, the present invention relates to a preparation method of a new polysaccharide compound contained in mussel, and also relates to the application of the glycoside compound in the preparation of antitumor drugs . Background technique [0002] Mussels are marine bivalve mollusks, commonly known as mussels and sea reds, and their dried products are collectively called mussels. In addition to rich amino acids, sugars, proteins and vitamins, mussels are also rich in trace elements - selenium. Shellfish extracts have anti-tumor, increase immune function, lower blood lipid, anti-aging, anti-virus and anti-bacterial functions; it has been recently reported that mussels have a high effect of repairing DNA damage, so it is expected to develop new components of anti-tumor drugs; mussels The mussel adhesive protein (glycoprotein) contained in the mucus protein secreted by podocytes is ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00
Inventor 张建鹏徐红丽卢小玲刘小宇刘军华冯伟华焦炳华
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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