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Partially acidic hydrolyzed compound of mytilus coruscus polysaccharides as well as preparation method and application of compound

A mussel polysaccharide and compound technology, applied in the field of medicine, can solve problems such as impact, complex digestion and absorption process, etc.

Inactive Publication Date: 2015-04-29
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a new partially acid-hydrolyzed polysaccharide compound of thick-shelled mussel and its preparation in view of the disadvantages of complex digestion and absorption process in the body of the polysaccharide compound due to its macromolecular properties, thereby affecting its biological function. Method; the second object of the present invention is to provide the application of the partially acid-hydrolyzed polysaccharide compound in the preparation of antitumor drugs

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1: Preparation of partially acid-hydrolyzed polysaccharides from thick-shelled mussels

[0025] Thick-shelled mussels were provided by Xiangyuan Aquatic Products Co., Ltd., Shengsi County, Zhejiang Province, and collected from the mussel breeding waters of Shengsi County, Zhejiang Province.

[0026] Take 700g of fresh mussel mollusk tissue, homogenize it with a tissue masher, add 1L of 1% methanol to soak for 24h to remove fat, and centrifuge at 5000rpm×10min to collect the precipitate. Add 5L of water, stir, boil for 5h, filter with suction, add water to the residue repeatedly, boil for 3h, filter with suction, and combine the two filtrates. The filtrate was concentrated by rotary evaporation at 50°C under reduced pressure. Decolorize with 0.5% activated carbon, and centrifuge to remove the precipitate. Add ethanol to a final concentration of 75%, and let stand at 4°C for alcohol precipitation overnight. The precipitate was collected by centrifugation, washe...

Embodiment 2

[0028] Embodiment 2: the research of the antitumor activity of the compound of the present invention

[0029] 1. In vitro tumor cell proliferation inhibition experiment

[0030] Human gastric cancer cell MKN-45 in the logarithmic growth phase was digested with 0.25% trypsin, and the cell concentration was adjusted to 2×10 5 / ml, seeded in 96-well plate, 6 replicate wells per group, 90 μl per well, 37°C, 5% CO 2 Adhere to the wall for 24 hours under the same conditions, add 10 μl of polysaccharide and partially acid hydrolyzed polysaccharide solution of different concentrations, so that the final concentration is 500 μg / ml, 100 μg / ml, 20 μg / ml, and set up negative control group (normal saline), positive control group ( 5-Fu) to a final concentration of 5 μg / ml. Incubate for 72 hours after sample addition, add 10 μl MTT (5 mg / m1) to each well, and continue to incubate for 4 hours. Carefully aspirate and discard the supernatant (discard the supernatant after the suspended cell...

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PUM

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Abstract

The invention relates to the technical field of medicines and provides a new partially acidic hydrolyzed compound of East China Sea mytilus coruscus polysaccharides. The invention also provides a preparation method and an application of the partially acidic hydrolyzed compound of the mytilus coruscus polysaccharides.

Description

technical field [0001] The present invention relates to the field of medical technology, in particular, the present invention relates to a new partially acid hydrolyzed compound of mussel polysaccharide, and at the same time, it also relates to a preparation method of the partially acid hydrolyzed polysaccharide compound and its application in the preparation of antitumor drugs Applications. Background technique [0002] Mussels are marine bivalve mollusks, commonly known as mussels and sea reds, and their dried products are collectively called mussels. In addition to rich amino acids, sugars, proteins and vitamins, mussels are also rich in trace elements - selenium. Shellfish extracts have anti-tumor, increase immune function, lower blood lipid, anti-aging, anti-virus and anti-bacterial functions; it has been recently reported that mussels have a high effect of repairing DNA damage, so it is expected to develop new components of anti-tumor drugs; mussels The mussel adhesiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A61K31/722A61P35/00
Inventor 张建鹏徐红丽卢小玲刘小宇刘军华冯伟华焦炳华
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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