The invention relates to an artificial culture method for Portunus trituberculatus in mariculture, and belongs to the technical field of agricultural mariculture. The artificial culture method consists of two parts of technology: 1, an integrated culture regulation method is carried out; and 2, anti-slaughter nets are arranged at the bottom of a pond, 300 nets are arranged in each mu and the slaughter on unshelled crabs by shelled crabs is reduced. The integrated culture regulation method comprises steps of: 1, performing pond regulation: dredging, sterilizing, drying sludge, fertilizing the fond, and laying pipelines at the bottom of the pond and sterilizing the water; 2, performing finite amount of water exchange; 3, regulating the water quality of the culture pond by using composite microorganism agents; 4, reasonable configuring and using aeration facilities; and 5, preparing feeds and reasonably feeding the feeds. Due to the application of the technology, the water quality of the culture pond is improved; the slaughter between the unshelled crabs and the shelled crabs is prevented; and the yield and economic benefit of the portunus trituberculatus are improved.