75results about How to "Improve seed quality" patented technology

The invention discloses a method for artificially breeding the seed of the spotted maigre, belonging to the field of mariculture in agriculture. In the method an indoor high-density seed breeding method is combined with a prelarva outdoor pond ecological breeding method. The method comprises the steps of: indoor spotted maigre prelarva high-density breeding, outdoor pond prelarva breeding, and fishing after the length of the prelarva is more than 5cm. The indoor spotted maigre prelarva high-density breeding comprises the steps of: 1. hatching, 2. breeding the prelarva, and 3. fishing and transporting. The outdoor pond prelarva breeding comprises the steps of: i. soil pond facility finishing and early preparing; and ii. soil pond breeding and managing. The soil pond facility finishing and early preparing comprises the steps of: 1. disinfecting the pond and the water body; 2. installing an oxygenating system; and 3. breeding biological bait. The soil pond breeding and managing comprisesthe steps of: 1. putting the prelarva; 2. changing the water; 3. putting the bait; and 4. regulating the water quality. The method combines with the advantages such as the high controllability of theindoor high-density seed breeding with the ecology imitation of the outdoor pond and the like, so that the seed breeding cost and risk are reduced, and the seed quality of the spotted maigre is improved, therefore, the method can be popularized and applied.

The invention provides methods and materials relating generally to plant derived flavin-containing monooxygenases (FMOs) capable of catalysing oxidation of a thio- to a sulphinyl-group during glucosinolate biosynthesis. It further relates to plant derived MYB factors capable of transcriptional regulation of biosynthetic genes. These have utility in the modification of glucosinolate biosynthesis.

The invention provides a stevia rebaudiana seeds coating agent and a pelleted coating method thereof. The stevia rebaudiana seeds coating agent comprises the following components in parts by weight: 10-20 parts of active nutrition pulvis, 50-70 parts of nonactive swelling pulvis, 15-25 partsof moulding pulvis, 10-20 parts of a nano brightener, 0.05-0.20 parts of a bactericide, 0.5-2.0 parts of a pesticide, and 0.02-0.05 parts of a nano water-retaining agent; wherein the granularity of the components is 1000-3000 meshes. The stevia rebaudiana seeds coating agent realizes breakthrough of a stevia rebaudiana seeds pelleted coating technology, is in favor of realizing mechanization sowing of the stevia rebaudiana during plantation and popularization processes, enables precision sowing, and overcomes the disadvantages of large consumption of manpower and material resource existed in a conventional plantation method.

The invention discloses a transgenic method of an agrobacterium-mediated rape field. The method comprises the following steps: eluting an agrobacterium on a YEB solid plate with 1 / 2 MS fluid nutrient media which comprises 5% of sucrose, 0.05-0.1% of Silwet-77, 0.2-0.4% of acetosyringone and 0.0001-0.0002% of 6-BAP by concentration; removing blooming flowers from a main inflorescence and each branched inflorescence and only leaving buds when the buds of a principal branch and part of lateral branches of the rape and a plurality of flowers bloom before the conversion; bundling the inflorescences immediately after soaking in eluted conversion bacterium liquid for 30 seconds; continuously dip-dyeing one inflorescence for 3 times with an interval of 2-3 days; and removing a parchment bag on the inflorescence and the flowers and buds at the top end of the inflorescence. The method has the advantages of simple operation, low cost, high transgenic efficiency and no genotype dependence, thereby having excellent potential application in the aspects of culturing rapes of new species by utilizing the gene engineering means and establishing rape mutant libraries.

The present invention belongs to the technical field of microorganisms and food biology, and particularly relates to a process for producing a dark tea by inoculating bred Eurotium cristatum, wherein the process comprises that Eurotium cristatum with a classification name of Eurotium cristatum NHRI-BC-1.5.1, preserved in the China General Microbiological Culture Collection Center, and with a preservation number of CGMCC No.8730 is adopted as a fermentation strain, and inoculation is performed after stacking or before vapor steaming or after metering step and before secondary vapor steaming. According to the present invention, the prepared dark tea product has characteristics of high quality and good stability, and the controllability of the Eurotium cristatum fermentation is basically achieved so as to further achieve the standardized control of the dark tea product.

The invention discloses an organic fragrant rice planting method which comprises the four key control steps of fragrant rice seed fertilizer coating processing, seedling growing, seedling transplanting and soil preparation and fertilizing. In the organic fragrant rice planting method, coated fertilizer is used for processing fragrant rice seeds, wherein the ratio between fertilizer and seed coating is 35-50:1. The effects of increasing the seed germination percentage, facilitating seedling growth and improving stress resistance are achieved after fertilizer coating is conducted. In the later period, organic fertilizer made of substance with natural insect-resistant and bacteriostatic activity is used, the dual effects of pesticide and fertilizer are achieved, plant diseases and insect pests are greatly reduced, and the later-period prevention frequency is reduced. In the fragrant rice heading period, leaf fertilizer made of seaweed extract is used for timely supplementing fertilizer needed in the heading period, and the photosynthetic efficiency is increased. The organic fragrant rice planting method has the advantages of being simple, saving time and labor, and obviously improving economical benefits, ecological benefits and social benefits.

The invention relates to the technical field of aquaculture, in particular relates to young sea cucumber raising techniques, and specifically relates to an ecological method for raising young sea cucumbers in offshore net cages. The method comprises the following steps of: making and putting raising net cages, selecting seed sea cucumbers, performing fertilization and hatching, raising larvae, raising young sea cucumbers, raising offshore in the winter, and stripping young sea cucumbers. The method selects natural sea areas for green ecological raising of young sea cucumbers, does not need any chemicals or artificial baits, utilizes abundant bait resources in natural sea areas, is energy-saving and environment-friendly, and achieves the purpose of natural and ecological raising of young sea cucumbers. The offshore raising method effectively reduces the raising cost, saves land resources, and reduces consumption of electrical energy and other energy sources. Besides, by virtue of natural selection and offshore overwinter, the quality of young sea cucumbers is improved and the aquaculture survival rate is increased.

The invention discloses a method for producing western wheatgrass seeds. In the method, 0.9-2.1kg / hm<2> of effective components of a plant growth regulator PP333 is sprayed on leaf surface of the western wheatgrass for 2-3 times every 5-7 days from late stage in turning green to the prophase of jointing of the western wheatgrass, thus greatly reducing plant height, effectively avoiding plant lodging and substantially improving the output and quality of the seed, which ensures that extensive application of the western wheatgrass in excellent pasture variety in revegetation and ecological conservation of deteriorated grassland, grassland agriculture and production practice of pasture grass seeds. The method has simple production method, low cost and simple operation, can substantially improve the output of the western wheatgrass seeds and the seed quality to some extent, and have good popularization and application prospect.

The invention discloses a seed treating method for improving the emergence rate of a camphor tree and provides a set of seed treating measures for treatment before seed harvesting, storage and seeding and particularly provides a physical and chemical treating method for accelerating sprouting of seeds. Compared with the traditional method, the method has the advantages that the seed quality of the camphor tree is obviously improved, the emergence rate is greatly improved, the quality of a grown nursery stock is excellent, a high-quality nursery stock foundation for building a camphor tree forest is provided, and the practical value is obvious.

The invention relates to a two-stage decomposition method. The method comprises the following steps: a discharged material of a two-stage decomposition end seed precipitation tank is pumped into a high-frequency multi-stage fine screen; an oversize product, namely, an aluminium hydroxide product, of the high-frequency multi-stage fine screen automatically flows into a horizontal pan filter, and then enters a roasting furnace, and an aluminium oxide product is obtained; a first-grade undersize product of the high-frequency multi-stage fine screen enters a secondary screen; a second-grade oversize product is added into a two-stage front decomposing tank as coarse seeds; a second-grade undersize product enters a fine seed settling tank; a seed precipitation mother solution overflows out of the fine seed settling tank, and is conveyed into an evaporation procedure; underflow of the fine seed settling tank flows into a fine seed filter; filtrate of the fine seed filter is returned to the fine seed settling tank; filter cakes of the fine seed filter are returned to a first-stage front seed precipitation tank as fine seeds. Compared with hydrocyclone classification, the method has the following advantages: the classification efficiency is high, the method is easy to control, the proportion of particles with the particle size smaller than 45 [mu]m in the aluminium oxide product is decreased, and the product conforms to the standard of sand-like aluminum oxide.

A method and a device are provided, in particular a transponder, for generating a random number by a linear feedback shift register, wherein a first seed is generated by means of a first automaton, a second seed is generated by means of a second automaton, the first and second automata operate using different functional principles, so that the first and second seeds have different properties, and the shift register is reloaded with the first and / or second seed.

The present invention relates to a method of predicting a plant seed trait, such as germination rate, vigour, aging and priming, by determining the presence of a target protein, i.e. diagnostic marker, in its active state in a protein sample derived from a plant seed or plant seed lot. Thereby, the quality of a plant seed or a plant seed lot can be predicted and / or diagnosed and seeds may be distinguished on basis of their characteristics and with respect to the traits described herein. Inter alia, seeds and seed lots having high germination quality may be distinguished from seeds and seed lots having low germination quality. In particular, the method comprises contacting the protein sample or the plant seed or plant seed lot with a chemical probe comprising a warhead being able to attach to an amino acid residue in or nearby an active site of the target protein under conditions allowing the formation of a conjugate of the target protein and the chemical probe, wherein the chemical probe further comprises a reporter tag which is used to detect the conjugate, and wherein detection of the conjugate indicates the presence of the target protein in the protein sample and is used to predict or diagnose the plant seed trait. The present invention further relates to the use of such chemical probes for the prediction of plant seed traits.

The invention discloses a treatment method for shortening oak seed germination time, and puts forward a whole set of seed treatment measures from seed harvesting, screening, storage to pre-sowing treatment. Compared with the traditional oak cultivation and seed treatment method, the method provided by the invention significantly improves the seed quality of oaks, greatly shortens the germination time, and substantially increases the emergence rate. Also, the germinated nursery stocks have excellent quality. The method overcomes the disadvantages of long germination time and low germination rate in traditional oak seeds, reduces the troubles in oak planting, provides high-quality nursery stocks for reforestation, and has significant practical value.

The invention provides an anther-stirring, pollen-collecting and hybrid pollination method of tobacco by using an artificial pollination pin. The method comprises specific steps that: during 8:00am to 10:00am or 4:00pm to 6:00pm, flowers of tobacco plants in post pollen tube growth periods are cut off and stored; flowers in blooming periods or blossom periods are selected as female parent flowers; during 8:00am to 9:00am or 5:00pm to 6:00pm on a sunny day, anther-stirring, pollen-collecting and hybrid pollination are carried out by using a pollination pin. According to the invention, male parent stamens and female parent pistils are in periods with optimal activities and affinities, such that pollination effect and seed quality are effectively improved; pollens in two pollen sacs of one anther are respectively used for pollinating two female parent flowers, and five anthers of a male parent flower can be used for pollinating 6 to 10 female parent flowers, such that a large amount of male parent pollens are saved, and the plantation amount of male parent tobacco is reduced; the pollination device is easy to manufacture and convenient to use, and the pollination method is simple, such that the seed yield is greatly improved, and the comprehensive cost of seed production is reduced. Therefore, the method is suitable to be applied in tobacco hybridization scientific experiments and tobacco large-area hybrid species propagation and production.

The present invention relates to the genetic manipulation of the expression of the AGL11 gene and homologs thereof in order to alter protein or oil levels in a seed of a crop plant. The downregulation of AGL11 is associated with an increase in protein concentration in the plant seed. Conversely, upregulating the AGL11 gene is associated with an increase in oil concentration in the plant seed.

A technology for overcoming the self-incompatibility of plant by laser includes such steps as picking up the mature anthers, drying for 1-3 hr until pollen is released, treating the pollen by laser, and pollinating within 1-5 hr. It can increase the setting percentage by 20-40%.

The invention relates to a hybrid corn seed production cost-saving, impurity-removing, emasculation and purity-keeping method, which comprises the following steps: adjusting the sowing time according to the planting and growth period characteristics of male parents and female parents and the flowering time of the male parents and the female parents, so that the spinning period of the female parents and the tassel pollination period of the male parents meet; performing abnormal plant identification by using a corn abnormal plant identification model after seed production corn male and female parent final singling, after jointing and before tasseling, and removing abnormal plants; in the female parent tasseling period, identifying tassels by using the corn tassel identification model, and removing the tassels which are extracted; removing tassel plants which are not completely removed in the female parents repeatedly, so that it is ensured that tassels of the female parents are completely removed after tasseling of the female parents and before pollination. In the corn seed production process, the labor intensity of manual impurity removal and female parent emasculation is relieved, influences of human factors are avoided, and the purity of hybrid corn seeds is improved.

The invention provides a seed breeding method for keeping spring sowing ecotype soybean variety seed nature stability. According to the method, by the aid of the characteristics of insensitive photoperiod response and high low-temperature resistance and weak-light resistance of spring sowing ecotype soybean varieties, greenhouse precocity planting of breeder seeds of spring sowing ecotype precocity soybean varieties is performed in February or March of tropical or subtropical areas to obtain S0 generation soybeans, the mature period of the breeder seeds of the spring sowing ecotype precocity soybean varieties is advanced to May or June, the S0 generation soybeans are planted in the areas in May or June, the conditions of the areas is characterized in that sunshine duration is 12h / d or more, average light intensity is larger than 500Lx and air humidity is lower than 20%, and S1 generation stock seed soybeans are acquired. According to the seed breeding method, seed vigor of the spring sowing ecotype soybean varieties is improved, the spring sowing ecotype soybean variety seed nature stability is kept, and the seed breeding method solves the problem of degeneration of the purity and the seed nature of the spring sowing ecotype soybean varieties caused by generation increase in seed propagation.

The invention relates to a corn three-line breeding technique, which comprises male sterile line A1 and A2, maintainer line B1 and B2, and restorer line AR1 and AR2, wherein during the production of hybrid seeds of corns, the complicated process step of artificial emasculation is omitted, and the production cost is reduced, and simultaneously the seed quality of hybrid seeds is improved. After seeding the hybrid seeds, the hybrid vigour is strong, and the increase in yield is significant. During the propagation and the production of the hybrid seeds of the three-line seeds, only by picking powder and performing artificial supplementary pollination in a propagation seed production area, the yield of propagating the three-line seeds and the hybrid seeds can be improved. Two isolation areas are selected to propagate the seeds of the male sterile line and the maintainer line, and the row ratio for culturing the male sterile line and the maintainer line is 4:1 or 6:1. One isolation area is used for propagating the seeds of the male sterile line and the maintainer line, while the other isolation area is used for producing the hybrid seeds and the restorer line seeds. The row ratio for culturing the hybrid seeds and the restorer line seeds is 4:1 or 6:1.

The invention provides a novel method for breeding kelp seedlings with a gametophyte cloning method, comprising the following steps of: respectively separating a female cell on a female gametophyte and a male cell on a male gametophyte under a microscope by using a capillary tube separation technology; then respectively cloning the female cell and the male cell by using a cloning vegetative propagation technology to respectively grow the female cell and the male cell into female and male gametophytes; continually breeding the gametophytes; when the gametophytes are bred to a fixed amount, damping a formed cell cluster by a cell stamping machine; mixing the female and male gametophytes and respectively releasing ova and sperms by the female and male gametophytes to spontaneously fertilize;putting fertilized zygotes into nutritive salt to be cultured; and after 10 weeks, obtaining seedlings with the height of about 1.5 cm. According to the invention, a fertilization process and the control of temperature and illumination are realized by manual operation, so that the growth period of offspring seeds is greatly shortened; meanwhile, the female cell and the male cell are separated from the gametophytes by using the capillary tube separation technology to clone and culture, so that the quality and the yield of the seedlings are improved, the cultivation of the kelp can be satisfied; and by utilizing the novel method, the cost for breeding the kelp seedlings is low.

The invention discloses a seed treatment method for improving quality of goldenrain tree seedlings and provides a whole set of seed treatment measure from seed harvesting and storage to treatment before seeding, in particular to a seed germination method with physical treatment and chemical treatment. Compared with a traditional method, the seed quality of goldenrain trees is obviously increased, the emergence rate is greatly increased, the quality of growing seedlings is excellent, the defect that a traditional goldenrain tree seed difficultly germinates is overcome, troubles during goldenrain tree planting are reduced, good seedlings are provided for afforestation, and a practical value is obvious.

The invention discloses a treatment method for shortening oak seed germination time, and puts forward a whole set of seed treatment measures from seed harvesting, screening, storage to pre-sowing treatment. Compared with the traditional oak cultivation and seed treatment method, the method provided by the invention significantly improves the seed quality of oaks, greatly shortens the germination time, and substantially increases the emergence rate. Also, the germinated nursery stocks have excellent quality. The method overcomes the disadvantages of long germination time and low germination rate in traditional oak seeds, reduces the troubles in oak planting, provides high-quality nursery stocks for reforestation, and has significant practical value.

The invention discloses a cross breeding method capable of increasing the fruiting rate of castanopsis hystrix. The cross breeding method comprises the following steps: S1, a female parent is subjected to castration and bagging; S2, when male flowers of a male parent are in the transitional period from an initial flowering period to a full-bloom period, male inflorescence of the male parent is collected and dried; S3, artificial pollination is performed: when stigmas of female flowers of the female parent are rolled reversely at the angle ranging from 40 degrees to 50 degrees, bags are removed on a sunny day, dry male flower pollen is sufficiently scattered on the stigmas of the female flowers from 9:00 to 10:30, and then bagging is performed; S4, the stigmas of the female flowers do not have mucus or get black 3-5 days after pollination, the bags are removed, the female flowers are exposed and grow naturally, and cross-bred seeds are obtained when green cones turn brown. With the adoption of the castanopsis hystrix cross breeding method, the fruit setting rate of castanopsis hystrix can be remarkably increased, the seed quality can be remarkably improved, the pollination success rate and the fruiting rate of castanopsis hystrix are increased efficiently, and besides, the method is simple to operate, easy to control and stable in effect. The cross breeding method has great significance in solving of the problem about lack of certified seeds of castanopsis hystrix.

A method and a device are provided, in particular a transponder, for generating a random number by a linear feedback shift register, wherein a first seed is generated by means of a first automaton, a second seed is generated by means of a second automaton, the first and second automata operate using different functional principles, so that the first and second seeds have different properties, and the shift register is reloaded with the first and / or second seed.

The invention provides an anther-stirring, pollen-collecting and hybrid pollination method of tobacco by using an artificial pollination pin. The method comprises specific steps that: during 8:00am to 10:00am or 4:00pm to 6:00pm, flowers of tobacco plants in post pollen tube growth periods are cut off and stored; flowers in blooming periods or blossom periods are selected as female parent flowers; during 8:00am to 9:00am or 5:00pm to 6:00pm on a sunny day, anther-stirring, pollen-collecting and hybrid pollination are carried out by using a pollination pin. According to the invention, male parent stamens and female parent pistils are in periods with optimal activities and affinities, such that pollination effect and seed quality are effectively improved; pollens in two pollen sacs of one anther are respectively used for pollinating two female parent flowers, and five anthers of a male parent flower can be used for pollinating 6 to 10 female parent flowers, such that a large amount of male parent pollens are saved, and the plantation amount of male parent tobacco is reduced; the pollination device is easy to manufacture and convenient to use, and the pollination method is simple, such that the seed yield is greatly improved, and the comprehensive cost of seed production is reduced. Therefore, the method is suitable to be applied in tobacco hybridization scientific experiments and tobacco large-area hybrid species propagation and production.

A crystal growth method, which includes the design of the compound mode of crystallization seeding process and continuous seeding process in sequence, the transition stage between the two processes adopts continuous seeding process, and the crystal pulling seeding process and continuous seeding process are combined Crystals are integrated together to create a composite seeding process that meets the actual needs of production, and according to the actual use of the seeding process, it can increase the production capacity of a single machine, shorten the seeding cycle, and reduce production costs.

The invention relates to a breeding method capable of controlling the aberration rate and the body color abnormity of verasper variegatus seedlings and belongs to the field of aquatic seedling culture. The method comprises the following steps of: feeding reinforced rotifer to fish larvae of verasper variegatus, which are at the age of 5 to 6 days; when the verasper variegatus grows to the age of 19 to 21 days, simultaneously feeding artemia nauplii and reducing the feeding quantity of the rotifer until the verasper variegatus grows to the age of 34 to 36 days; and then starting to completely feed the artemia nauplii, wherein the artemia nauplii is reinforced by schizochytrium limacinum and the rotifer is reinforced by se-yeast after being reinforced by the schizochytrium limacinum. According to the invention, the Se-yeast is used as a reinforcer of the rotifer; content of selenium in a bait is improved; the fish larvae of verasper variegatus can be promoted to grow; the metamosphsis rate of the fish larvae of verasper variegatus is promoted; the aberration rate and the ratio of the body color abnormity are effectively reduced; survival rate is high; and the seedlings have high quality.

The invention belongs to the field of sugar industry, and discloses a sucrose seed crystal and a preparation method thereof. The preparation method comprises the steps: dissolving cane sugar in waterto prepare a sugar solution with the mass concentration of 50%-70%, and then concentrating the sugar solution at the temperature of -10 DEG C to 4 DEG C to obtain a concentrated sugar solution with the cane sugar mass concentration of 82%-90%; and adding ethanol into the concentrated sugar solution, treating for 5-120 s under an ultrasonic condition, filtering, collecting, dispersing and preserving to obtain the sucrose seed crystal. According to the method, the seed crystal is prepared under the low-temperature condition, sucrose conversion can be avoided, and the seed crystal quality is improved. The obtained seed crystal is uniform in granularity and smooth in surface. The reduction of treatment difficulty of subsequent procedures is facilitated and improvement of the quality of sucrosecrystal products is facilitated.

A method for cultivating a cauliflower hybrid by using a male sterile line comprises the following steps: transferring male sterile lines to obtain sterile lines and cauliflower male sterile lines with the sterility degree of 100%; screening out various male sterile lines with different target properties, and matching the male sterile lines as a female parent with a corresponding self-variable line male parent according to a complementation principle to prepare a hybrid combination; and carrying out field identification flying variety comparison test and production test to obtain the new hybrid with target properties. The method has the following advantages: 1, the hybrid rate can reach 100%; 2, continuous selfing is not needed, so decline of the vitality of parent plants is avoided; 3, the field seed production is simple, the manpower is reduced, the production cost is reduced, and promotion is easy; and 4, the cultivated male sterile line is not plagiarized in the seed production process, so the intellectual property rights of a seed producer are protected to a certain degree.

The invention provides a composite feed additive capable of improving the juvenile large yellow croaker digestive enzyme activity and promoting intestinal tract development. The composite feed additive is prepared from, by mass, 1-15% of tributyrin, 2-6% of astaxanthin and 80-95% of yeast extract powder. The composite feed additive is applied as a juvenile large yellow croaker feed additive. The functional additive can remarkably improve the juvenile large yellow croaker digestive absorption capability, the juvenile fish survival rate is increased, and a favorable foundation is laid for improving the large yellow croaker fry quality. Meanwhile, the additive is of a great significance in perfecting the juvenile large yellow croaker cooperative feed, accordingly, the feed additive is worthyof being applied and popularized, and larger economic benefits are created.