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Anther-stirring, pollen-collecting and hybrid pollination method of tobacco by using artificial pollination pin

A technology of hybrid pollination and tobacco production, which is applied in the fields of botany equipment and methods, applications, plant genetic improvement, etc., can solve the problems that pollen cannot be fully utilized, the production cost of hybrids is high, and the amount of male parent flowers is wasted, etc., to achieve Reduce the amount of planting, improve the pollination effect, and save the pollen of the male parent

Inactive Publication Date: 2012-02-29
TOBACCO RES INST OF HUBEI PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the following disadvantages: one is that the male parent flower has already bloomed, some of the pollen is scattered, and the pollen that can be pollinated is less. Can not be fully utilized, the male parent flower waste is large, and the planting area of ​​tobacco plants is large; the third is the high production cost of hybrids, poor pollination efficiency, and poor pollination effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment one, taking tobacco hybrid seed propagation as an example, its specific steps are as follows:

[0023] A male parent flower picking, at 8-10 am or 4-6 pm, the pollen tube is elongated, the flower head is partially swollen, the flower tube mouth is slightly pink, the corolla is about to open but not yet open, the pollen is fully developed but mature The flowers that are not scattered and are in the late stage of the elongation of the flower tube of the tobacco plant are gently cut from the flower stalk, and then put into the fresh-keeping equipment for temporary storage, and the storage time does not exceed 24 hours;

[0024] B Prepare pollination utensils, make pollination hooks with semicircular, 2-3cm in diameter, No. 8-10 iron wire, or use other material hooks of the same size. There is a thin rope of about 60-80cm at the end of the pollination hook; the pollination needle is made of bamboo, wood and weed stems, the length is about 10cm, and the diameter o...

Embodiment 2

[0033] Embodiment two, in 2009, Eyan No. 1 (the main species of Burley tobacco in Hubei Province) was tested at the China Tobacco Enshi Breeding Breeding Base, compared the pollination method of the present invention and the flower-to-flower pollination method, and the statistics show that : The seed yield of flower-to-flower pollination method is 5.52kg / mu, the early production cost of tobacco plants is 3088.00 yuan / mu, the cost of pollination period is 2785.00 yuan / mu, the pollination cost of producing 1kg seeds is 504.53 yuan, the early production cost of female parent is 559.42 yuan, The production cost of this product is 236.08 yuan, and the comprehensive cost is 1300.02 yuan; the pollination method seed yield of the present invention is 11.65kg / mu, and the early production cost of tobacco plants is the same as that of the flower-to-flower method, and the pollination period cost is 4224.56 yuan / mu, producing 1kg The pollination cost of seeds is 362.62 yuan, the production ...

Embodiment 4

[0035] Embodiment four, in 2010, Burley tobacco MSTN90 was tested at China Tobacco Enshi Breeding Breeding Base, compared with the pollination method of the present invention and the flower-to-flower pollination method, the test data showed: the flower-to-flower pollination method seed yield 4.65kg / mu , the initial production cost of tobacco plants is 3463.37 yuan / mu, the cost of pollination period is 2738.27 yuan / mu, the pollination cost of producing 1kg seeds is 588.88 yuan, the early production cost of female parent is 744.81 yuan, the production cost of male parent is 297.92 yuan, and the comprehensive cost is 1631.61 yuan; Adopt the pollination method of the present invention, seed yield 9.58kg / mu, the early stage production cost of tobacco plant is the same as flower-to-flower method, the cost of pollination period is 4361.31 yuan / mu, the pollination cost of producing 1kg seed is 455.31 yuan, female parent early stage production Cost 361.52 yuan, the production cost of ma...

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Abstract

The invention provides an anther-stirring, pollen-collecting and hybrid pollination method of tobacco by using an artificial pollination pin. The method comprises specific steps that: during 8:00am to 10:00am or 4:00pm to 6:00pm, flowers of tobacco plants in post pollen tube growth periods are cut off and stored; flowers in blooming periods or blossom periods are selected as female parent flowers; during 8:00am to 9:00am or 5:00pm to 6:00pm on a sunny day, anther-stirring, pollen-collecting and hybrid pollination are carried out by using a pollination pin. According to the invention, male parent stamens and female parent pistils are in periods with optimal activities and affinities, such that pollination effect and seed quality are effectively improved; pollens in two pollen sacs of one anther are respectively used for pollinating two female parent flowers, and five anthers of a male parent flower can be used for pollinating 6 to 10 female parent flowers, such that a large amount of male parent pollens are saved, and the plantation amount of male parent tobacco is reduced; the pollination device is easy to manufacture and convenient to use, and the pollination method is simple, such that the seed yield is greatly improved, and the comprehensive cost of seed production is reduced. Therefore, the method is suitable to be applied in tobacco hybridization scientific experiments and tobacco large-area hybrid species propagation and production.

Description

technical field [0001] The invention relates to a method for artificial cross-pollination of crops, in particular to a method for artificial cross-pollination of tobacco with a needle for picking medicine and taking powder. Background technique [0002] Artificial cross-pollination is the basic method of breeding new varieties by means of hybridization in plant scientific research work, and it is also the only way to obtain excellent hybrid fruits and seeds in the process of large-scale plant production. However, the method of cross-pollination is also different because of the different plant species and their flower organ structures. [0003] Tobacco flowers are bisexual complete flowers, 5 petals united into a tubular corolla, the stigma of the pistil is connected to the style in the center of the corolla; 5 stamens are distributed around the pistil, and each anther of the stamen consists of two anthers, kidney-shaped, and the back is connected to the filament . Since my...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 李宗平李进平郭宇龙徐世平王文明张俊杰陈茂胜彭灏
Owner TOBACCO RES INST OF HUBEI PROVINCE
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