63 results about "Chemical probe" patented technology

A method of fabricating an addressable array of chemical probes at respective feature locations on a substrate surface. The method may use a deposition apparatus with a substrate unit which includes the substrate and with a drop deposition unit which includes a drop deposition head. Such an apparatus when operated according to a target drive pattern based on nominal operating parameters of the apparatus provides the probes on the substrate surface in the target array pattern. The method may include depositing at least one drop from the head unit onto the substrate surface. A fiducial on the substrate unit is optionally viewed from a sensor. A deposited drop on the substrate surface is viewed from a sensor. An actual position of the viewed deposited drop may be determined relative to a fiducial on the substrate unit, based on the views of the fiducial and deposited drop. An error is determined based on any difference between the actual and target positions. Alternatively, an error for one or more deposition units may be determined based on a statistical difference between the actual and target positions of a set of multiple drops deposited from each deposition unit. The deposition apparatus is operated to deposit further drops from the head unit onto the substrate surface at feature locations while moving at least one of the substrate unit or head unit with respect to the other, so as to fabricate the array. Apparatus and computer program products are also provided.

The invention relates to a preparation method of an aminated nanofiber membrane with a high specific surface area. The preparation method comprises the following steps of: dissolving two polymer materials with the weight ratio of 1: (0.1-10) or dissolving a polymer material and an inorganic salt into a solvent, dissolving while stirring at the temperature of 20-80 DEG C for 4-96h, and carrying out electrostatic spinning to obtain a composite nanofiber membrane; soaking the composite nanofiber membrane into water, regulating the pH value, soaking at the temperature of 20-95 DEG C for 2-48h, washing and drying to obtain a nanofiber membrane with a porous micro / nano structure and a high specific surface area; and immersing the nanofiber membrane with a porous micro / nano structure and a high specific surface area into water, adding an amination reaction reagent to carry out amination reaction at the temperature of 50-200 DEG C, then, taking out the fiber membrane, washing the fiber membrane to be neutral, and drying the fiber membrane to obtain the aminated nanofiber membrane with a high specific surface area. The product provided by the invention is applied to fields such as adsorption and separation of precious metal ions, heavy metal ions and transition metal ions, chemical probes, sensors, environment monitoring, catalysts, biological medicines and the like.

The invention relates to a piece of surface enhanced raman detection test paper and the application thereof, which belong to the technical field of ultra-sensitive test analysis, in particular to the piece of surface enhanced raman detection test paper and the application of the paper to detection of biological or chemical probe molecules with raman signals. The surface enhanced raman detection test paper is obtained by covering a precious metal layer on the surface of paper with a natural fiber micro nano multi-grade structure through the physical vapor deposition or chemical plating technology, the paper can be filter paper, parchment paper, napkin, newspaper or printing paper and the like, the precious metal film is gold or silver and the like, and the thickness of the precious metal film is 5 nanometers to 90 nanometers. Combined action of size, period and roughness enables incident light to generate a local electric field on the surface of a multi-stage structure and enables the electric field to be enhanced, and the detection limit can reach 10-10mol / L. The test paper has the advantages of being good in flexibility, low in cost, free of environment pollution, capable of being prepared in batch and the like, thereby being capable of being used in detection of probe molecules such as rhodamine 6G, p-aminothiophenol, riboflavin or ethanol and the like.

A method and apparatus are provided for aligning optical elements or microbeads, wherein each microbead has an elongated body with a code embedded therein along a longitudinal axis thereof to be read by a code reading device. The microbeads are aligned with a positioning device so the longitudinal axis of the microbeads is positioned in a fixed orientation relative to the code reading device. The microbeads are typically cylindrically shaped glass beads between 25 and 250 microns (μm) in diameter and between 100 and 500 μm long, and have a holographic code embedded in the central region of the bead, which is used to identify it from the rest of the beads in a batch of beads with many different chemical probes. A cross reference is used to determine which probe is attached to which bead, thus allowing the researcher to correlate the chemical content on each bead with the measured fluorescence signal. Because the code consists of a diffraction grating typically disposed along an axis, there is a particular alignment required between the incident readout laser beam and the readout detector in two of the three rotational axes. The third axis, rotation about the center axis of the cylinder, is azimuthally symmetric and therefore does not require alignment.

The invention relates to a preparation method of a dopamine composite nano-fiber affinity membrane for adsorbing La3+. The preparation method comprises the steps of (1) dissolving polymer materials in a solvent to obtain a polymer blended solution with the mass percentage concentration of 8%-30%, and then obtaining a composite nano-fiber membrane through electrostatic spinning, and (2) soaking the composite nano-fiber membrane in a Tris-HCl solution of dopamine, and obtaining the dopamine composite nano-fiber affinity membrane for adsorbing the rare-earth metal ion La3+. The preparation method is simple and practical, raw materials can be easily obtained, the cost is low, the dopamine composite nano-fiber affinity membrane for adsorbing the La3+ can be conveniently and accurately prepared, and the large-scale production operation can be easily achieved. According to the preparation method, the dopamine composite nano-fiber affinity membrane with the high specific surface area can be widely applied to the fields such as rare-earth metal La3+ gathering, rare-earth metal La3+ adsorbing, rare-earth metal La3+ separation, chemical probes, sensors and water treatment.

The present disclosure relates to a method for metabolic oligosaccharide engineering that incorporates derivatized alkyne-bearing sugar analogs as “tags” into cellular glycoconjugates. The disclosed method incorporates alkynyl derivatized Fuc and alkynyl derivatized ManNAc sugars into a cellular glycoconjugate. A chemical probe comprising an azide group and a visual probe or a fluorogenic probe is used to label the alkyne-derivatized sugar-tagged glycoconjugate. In one aspect, the chemical probe binds covalently to the alkynyl group by Cu(I)-catalyzed [3+2] azide-alkyne cycloaddition and is visualized at the cell surface, intracellularly, or in a cellular extract. The labeled glycoconjugate is capable of detection by flow cytometry, SDS-PAGE, Western blot, ELISA or confocal microscopy, and mass spectrometry.

The present disclosure relates to a method for metabolic oligosaccharide engineering that incorporates derivatized alkyne-bearing sugar analogs as “tags” into cellular glycoconjugates. The disclosed method incorporates alkynyl derivatized Fuc and alkynyl derivatized ManNAc sugars into a cellular glycoconjugate. A chemical probe comprising an azide group and a visual probe or a fluorogenic probe is used to label the alkyne-derivatized sugar-tagged glycoconjugate. In one aspect, the chemical probe binds covalently to the alkynyl group by Cu(I)-catalyzed [3+2] azide-alkyne cycloaddition and is visualized at the cell surface, intracellularly, or in a cellular extract. The labeled glycoconjugate is capable of detection by flow cytometry, SDS-PAGE, Western blot, ELISA or confocal microscopy, and mass spectrometry.

A biological or chemical optical sensing device comprises at least one planar micro-resonator structure included in a light emitting waveguide, and at least one biological or chemical probe bound to at least a part of the micro-resonator structure, the probe operative to bind specifically and selectively to a respective target substance, whereby the specific and selective binding results in a parameter change in light emitted from the waveguide. In one embodiment, the micro-resonator is linear. In some embodiments, the sensing device is active, the waveguide including at least one photoluminescent material operative to be remotely pumped by a remote optical source, and the parameter change, which may include a spectral change or a quality-factor change, may be remotely read by an optical reader.

A biological or chemical optical sensing device comprises at least one planar micro-resonator structure included in a light emitting waveguide, and at least one biological or chemical probe bound to at least a part of the micro-resonator structure, the probe operative to bind specifically and selectively to a respective target substance, whereby the specific and selective binding results in a parameter change in light emitted from the waveguide. In one embodiment, the micro-resonator is linear. In some embodiments, the sensing device is active, the waveguide including at least one photoluminescent material operative to be remotely pumped by a remote optical source, and the parameter change, which may include a spectral change or a quality-factor change, may be remotely read by an optical reader.

The invention discloses a thiamphenicol molecularly imprinted electrochemical sensor and a preparation method and application thereof. A porous graphene-molybdenum disulfide nano flower-shaped compound is prepared through a hydrothermal one-step method, and the compound is dropped on the surface of an L-type glassy carbon electrode; aminated multi-walled carbon nanotubes and porous Pt-Pd nanoparticles are dispersed so that the imprinting surface area of the electrode can be increased and stability of a modification interface can be enhanced, and accordingly a three-dimensional porous imprinting substrate is obtained; thiamphenicol serves as template molecules, 1,2-diaminobenzene serves as functional monomers, and an imprinted membrane is prepared through cyclic voltammetry; ascorbic acid serves as a photoelectric chemical probe, and the electrochemical sensor for detecting thiamphenicol is built. The sensor has good thiamphenicol responsiveness, the linear range of the sensor is 1.0*10<-9>-3.5*<-6> mol L<-1>, and the detection lower limit is 5.0*10<-9> mol L<-1>. The sensor can be used for detecting thiamphenicol in meat samples and feed samples.

A method and apparatus for probing the chemistry of a single droplet are provided. The technique uses a variation of the field-induced droplet ionization (FIDI) method, in which isolated droplets undergo heterogeneous reactions between solution phase analytes and gas-phase species. Following a specified reaction time, the application of a high electric field induces FIDI in the droplet, generating fine jets of highly charged progeny droplets that can then be characterized. Sampling over a range of delay times following exposure of the droplet to gas phase reactants, the spectra yield the temporal variation of reactant and product concentrations. Following the initial mass spectrometry studies, we developed an experiment to explore the parameter space associated with FIDI in an attempt to better understand and control the technique. In an alternative embodiment of the invention switched electric fields are integrated with the technique to allow for time-resolved studies of the droplet distortion, jetting, and charged progeny droplet formation associated with FIDI.

Chemical stain compounds containing a fluorophore and a reducible quenching unit are disclosed. The reducible quenching unit quenches the fluorophore while in its oxidized state. Upon reduction, the quenching properties of the quenching unit are diminished or eliminated. The chemical compounds can be used in a variety of applications, including the detection of bacterial cells, monitoring the electron transport chain function of bacterial cells, monitoring the oxidation state of non-biological systems, and assaying the effectiveness of antibacterial or antimicrobial agents.

The invention discloses compounds 1 and 2 of the following structural formula, pharmaceutical compositions containing the compounds as effective components, a preparation method of the two compounds, and application of the two compounds for preparing protein 14-3-3 small molecule chemical probes and preparing pharmaceuticals for treating cancers or nerve degenerative diseases relates to over-expression of protein 14-3-3. The compounds 1 and 2 have a significant inhibitory effect on protein 14-3-3.

A method for control data quality of automatic buoy monitoring system relates to an automatic buoy monitoring system. Data quality analysis, before data quality control, needs to analyze the data quality. Data quality analysis is an important link in the data preparation process of buoy data characteristic analysis, and is the prerequisite of data pretreatment. Outlier elimination method, aiming at the outlier obtained by the automatic monitoring system; on-site validation and evaluation: validate and evaluate the monitoring parameters of the buoy through on-site sampling and laboratory measurement, especially the data measured by biological and chemical probes, to ensure the reliability of the data; The comparison parameters mainly include NO3, DO, Chl and CDOM. Water samples can be collected at the place where the buoys are placed and brought back to the laboratory for analysis; then the correlation curve between the laboratory analysis data and the buoy data is drawn to judge the reliability of the buoy data from its distribution trend.

The invention relates to the technology of phosphorescence chemical probe, in particular to a water-soluble cationic iridium complex phosphorescence probe and a preparation method. Iridium metal is not used for cell marking in the prior art. The structural formula of the water-soluble cationic iridium complex phosphorescence probe of the invention is as shown in the attached figure. The preparation of the invention is as follows: preparing 2-phenylpyridine iridium dichlorendic complex Ir2 (ppy) 4Cl2; preparing 3, 8-diacetylene o-phenanthroline; preparing 3, 8-diacetylene o-phenanthroline iridium 2-phenylpyridine complex; and preparing the objective complex. The invention has the advantages that with good water solubility and lipid solubility, the complex can quickly enter cells; and with long luminescent lifetime of iridium complex, the interference of the background fluorescence can be effectively eliminated through the time-resolved fluorescence technique, and thereby improving the detection sensitivity can be improved.

A method and apparatus for probing the chemistry of a single droplet are provided. The technique uses a variation of the field-induced droplet ionization (FIDI) method, in which isolated droplets undergo heterogeneous reactions between solution phase analytes and gas-phase species. Following a specified reaction time, the application of a high electric field induces FIDI in the droplet, generating fine jets of highly charged progeny droplets that can then be characterized. Sampling over a range of delay times following exposure of the droplet to gas phase reactants, the spectra yield the temporal variation of reactant and product concentrations. Following the initial mass spectrometry studies, we developed an experiment to explore the parameter space associated with FIDI in an attempt to better understand and control the technique. In an alternative embodiment of the invention switched electric fields are integrated with the technique to allow for time-resolved studies of the droplet distortion, jetting, and charged progeny droplet formation associated with FIDI.

The invention relates to a preparing method of a DNA determining electrochemical sensor based on a platinum nano particle catalysis electrochemistry circulation signal amplification technology and the application of the electrochemical sensor. A hairpin DNA 1 is assembled on the surface of a platinum nano particle modified gold electrode in a self-assembly mode, and when a target DNA 2 exists, due to the fact that the target DNA 2 and the hairpin DNA 1 assembled on the surface of the gold electrode are partially paired in a complementary mode, the hairpin structure of the DNA 1 is opened. Then, another hairpin DNA 3 chain replaces the target chain DNA 2 by competing pairing hybridization, the target chain DNA 2 is released, and the released target chain is continuously opened to form a new hairpin structure. The action is carried out in a cycling mode, recycling of the target chain DNA 2 is achieved, a prepared nano particle electrochemical probe is combined, signal amplification is achieved, the prepared electrochemical sensor is used for catalyzing cycling between PAP and PQI, further electrochemistry signal amplification is achieved, a cyclic voltammetry or DPV is used for determining current signal intensity, and according to the measured current signal intensity, determining on the target chain DNA2 is achieved. The sensor has high detecting sensitivity.

A metal oxide supported palladium catalyst comprised of a β-Bi2O3 / Bi2Sn2O7 hetero-junction catalyst support and palladium was developed. The catalyst was synthesized using a sol-gel technique as a nanocrystalline structure. In the presence of fluorene, an oxidant and ultraviolet irradiation, the catalyst converts the hydrocarbon to a mixture of fluorenol / fluorenone oxidation products. The close proximity between β-Bi2O3 and Bi2Sn2O7 heterojunction phases in the catalyst is thought to be responsible for the efficient charge separation and catalytic activity. An indirect chemical probe method using active species scavengers elucidated that the photo-oxidation mechanism proceeds via holes and superoxide radical (O2.−) moieties.

The invention relates to a non-natural amino acid compound represented by a general formula (I) and a preparation method thereof, and applications of the non-natural amino acid compound in fixed-pointmodification of bio-macro-molecular proteins, protein interaction and biological research. Specifically, the non-natural amino acid provided by the invention is used as a chemical probe with a novelstructure, and is used for protein crosslinking, research of protein-protein interaction, site-specific labeling of protein and application of protein site-specific modification.

Chemical stain compounds containing a fluorophore and a reducible quenching unit are disclosed. The reducible quenching unit quenches the fluorophore while in its oxidized state. Upon reduction, the quenching properties of the quenching unit are diminished or eliminated. The chemical compounds can be used in a variety of applications, including the detection of bacterial cells, monitoring the electron transport chain function of bacterial cells, monitoring the oxidation state of non-biological systems, and assaying the effectiveness of antibacterial or antimicrobial agents.

The present invention provides a method of optical electrophysiological probing, including: providing a fluorescing chemical probe; contacting a thick portion of tissue with the fluorescing chemical probe to create a thick portion of treated tissue; applying a first range of wavelengths of electromagnetic radiation to the treated portion of tissue; and detecting a plurality of depth-specific emission wavelengths emitted from the thick portion of treated tissue.

The invention discloses a perylene diimide derivative, a preparation method and application of the perylene diimide derivative in preparation of an ATP (adenosine triphosphate) fluorescent probe. Theperylene diimide derivative is shown as a formula (IV), wherein R<+> = R'2(CH3)2N<+>, and R' is linear alkyl with 16-18 carbon atoms, branched alkyl or alkyl with a cyclic alkyl chain. The ATP fluorescent probe prepared from the perylene diimide derivative can selectively and quickly detect ATP, the prepared ATP fluorescent probe is good in chemical stability, high in sensitivity and good in selectivity and can be combined with a commercial fluorescent instrument to achieve quick response and real-time monitoring of the ATP; the perylene diimide derivative is used for preparing the fluorescentprobe high in luminescence performance and capable of quickly selectively analyzing the ATP, the preparation method is simple and convenient, reaction conditions are mild, reaction is performed in ethanol water, environment pollution is avoided, the requirements of green chemical probes are met, synthesis is simple, and products are easy to separate and purify.

The present invention discloses a water-soluble cationic conjugated microporous polymer phosphorescent probe and a preparation method thereof. 2-(4-bromophenyl) pyridine iridium dichloride bridged complex Ir2(Brppy)4Cl2, 3,8-dibromo phenanthroline and 3,8-dibromo phenanthroline iridium 2-(4-bromophenyl) complex are sequentially prepared to prepare an objective polymer. The polymer chemical probe prepared through the above technical scheme has good water solubility and can bind with nucleic acid through electrostatic interaction; the probe has good phosphorescence emission in water and long luminescence lifetime; and a time-resolved fluorescence technique can effectively eliminate interference of the background fluorescence and improve detection sensitivity. The preparation preparation method has the advantages of simple operation and easily available raw materials, and is beneficial to reduce the cost.

The present invention relates to a method of predicting a plant seed trait, such as germination rate, vigour, aging and priming, by determining the presence of a target protein, i.e. diagnostic marker, in its active state in a protein sample derived from a plant seed or plant seed lot. Thereby, the quality of a plant seed or a plant seed lot can be predicted and / or diagnosed and seeds may be distinguished on basis of their characteristics and with respect to the traits described herein. Inter alia, seeds and seed lots having high germination quality may be distinguished from seeds and seed lots having low germination quality. In particular, the method comprises contacting the protein sample or the plant seed or plant seed lot with a chemical probe comprising a warhead being able to attach to an amino acid residue in or nearby an active site of the target protein under conditions allowing the formation of a conjugate of the target protein and the chemical probe, wherein the chemical probe further comprises a reporter tag which is used to detect the conjugate, and wherein detection of the conjugate indicates the presence of the target protein in the protein sample and is used to predict or diagnose the plant seed trait. The present invention further relates to the use of such chemical probes for the prediction of plant seed traits.

The invention relates to a method for quickly searching a basin concealed sandstone-type uranium mine. The method comprises the steps of collecting the geological and gravity data of the basin uraniummine, and researching the ore control structure features of the sandstone type uranium mine; carrying out aviation gamma energy spectrum measurement to obtain an original counting rate of the uraniumwindow; correcting and converting the counting rate of the original uranium window, solving the uranium content, and screening aerial uranium abnormities and high fields; carrying out ground verification on the abnormal or high field, collecting a soil chemical probe sample, analyzing the contents of uranium and radium elements, and solving a uranium and radium balance coefficient; performing constraint by using the uranium radium balance coefficient, calculating the radium content of the measurement area by using aerial uranium content data, and screening radium anomalies and high fields; and making a radium anomaly and high-field distribution diagram, and delineating a sandstone type uranium mine prospecting favorable area in combination with the ore control structure. According to themethod, radium abnormal information of the deep-source uranium decay daughter can be effectively extracted, the basin concealed sandstone type uranium mine favorable area can be quickly delineated, and the method is provided for searching for the concealed uranium mine in the basin.

The invention relates to a preparation method of an aminated nanofiber membrane with a high specific surface area. The preparation method comprises the following steps of: dissolving two polymer materials with the weight ratio of 1: (0.1-10) or dissolving a polymer material and an inorganic salt into a solvent, dissolving while stirring at the temperature of 20-80 DEG C for 4-96h, and carrying out electrostatic spinning to obtain a composite nanofiber membrane; soaking the composite nanofiber membrane into water, regulating the pH value, soaking at the temperature of 20-95 DEG C for 2-48h, washing and drying to obtain a nanofiber membrane with a porous micro / nano structure and a high specific surface area; and immersing the nanofiber membrane with a porous micro / nano structure and a high specific surface area into water, adding an amination reaction reagent to carry out amination reaction at the temperature of 50-200 DEG C, then, taking out the fiber membrane, washing the fiber membrane to be neutral, and drying the fiber membrane to obtain the aminated nanofiber membrane with a high specific surface area. The product provided by the invention is applied to fields such as adsorption and separation of precious metal ions, heavy metal ions and transition metal ions, chemical probes, sensors, environment monitoring, catalysts, biological medicines and the like.

The invention relates to a preparation method of a disulfide dot / nano sheet composite DNA electrochemical probe. The method specifically includes the following steps: 1) ultrasonically stripping the transition metal disulfide powder, high-temperature electromagnetic stirring to prepare a transition metal disulfide quantum dot / nanosheet composite, ultrasonically in a dispersion of thionine and an ionic liquid, Prepare a thionine functionalized complex; 2) Disperse the prepared complex in an organic solvent, make a suspension and drop-coat it on the electrode, and obtain a complex modified electrode after drying; 3) Prepare the above prepared electrode In the three-electrode system, the electrochemical test is performed in the buffer solution, and DNA solutions of different concentrations are injected for testing, and the electrochemical signal changes on the electrode surface are recorded to construct a DNA electrochemical probe system. The probe of the invention has the advantages of high sensitivity, good stability, wide linear range, low detection limit, etc., can be used for detecting DNA in serum, and has broad application prospects in the fields of biological analysis and clinical diagnosis.

The present invention provides a method of optical electrophysiological probing, including: providing a fluorescing chemical probe; contacting a thick portion of tissue with the fluorescing chemical probe to create a thick portion of treated tissue; applying a first range of wavelengths of electromagnetic radiation to the treated portion of tissue; and detecting a plurality of depth-specific emission wavelengths emitted from the thick portion of treated tissue.

The invention discloses a potential-resolved electrochemiluminescence-based antigen detection method. The method comprises the steps of: preparing an electrochemiluminescent probe; doping the electrochemiluminescent probe into a polymer microsphere; marking a detection antibody onto the surface of the polymer microsphere so as to obtain a double-encoded polymer microsphere double of the detectionantibody and the electrochemiluminescent probe; covalently marking a capture antibody to the surface of a magnetic microsphere so as to obtain an immunomagnetic microsphere marked by the capture antibody; placing the double-encoded polymer microsphere and the immunomagnetic microsphere into to-be-detected solution, carrying out magnetic separation, and releasing an electrochemical probe by swelling the polymer microsphere; acquiring electrochemical luminescence signals of the electrochemical probe at different potentials to identify the detected antigen. The antigen detection method provided by the invention is capable of improving the defect that the single-component electrochemiluminescence immunoassay is long in time and low in flux, and realizing rapid, high-throughput and high-sensitivity joint detection of various tumor markers, thereby being suitable for developing kits for target tumors for large-scale early screening of tumors.