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Novel wide-spectrum bacillus resisting clinical and plant pathogenic fungi and application thereof

A plant pathogenic and antifungal technology, applied in the biological field, can solve problems such as no research and report on antifungal effects, and achieve the effects of obvious bacteriostatic effect, broad antifungal spectrum, and simple fermentation medium.

Inactive Publication Date: 2013-05-08
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these bacillus have obvious effects mainly against plant filamentous pathogenic fungi, but there are no studies and reports on antifungal effects on human superficial fungi and deep fungal pathogens

Method used

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  • Novel wide-spectrum bacillus resisting clinical and plant pathogenic fungi and application thereof
  • Novel wide-spectrum bacillus resisting clinical and plant pathogenic fungi and application thereof
  • Novel wide-spectrum bacillus resisting clinical and plant pathogenic fungi and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Identification of Bacteria

[0019] Bacillus sp.AH-E-1 was identified by morphological methods, traditional physiological and biochemical methods and 16s rRNA gene sequencing methods.

[0020] (1) Morphological observation

[0021] Streak Bacillus sp.AH-E-1 on the nutrient agar plate, and observe the colony morphology after culturing at 37°C for 24 hours. It can be seen that the colony is round, white, with dry surface and rough edges. After picking a single colony for staining, observe the morphology of the bacteria under a microscope, and it can be seen that ( figure 1 ): The strain is Gram-positive, with spores, the spores are oval or cylindrical, not obviously enlarged, the spores are mesozoic, and the ends of the bacteria are round.

[0022] (2) Physiological and biochemical identification

[0023] Bacillus cereus (CGMCC1.229), Bacillus subtilis (CGMCC1.892), Bacillus pumilus (CGMCC1.894), Bacillus amyloliquefaciens (CGMCC 1.1099), Bacillus thuringien...

Embodiment 2

[0029] Embodiment 2: the mensuration of antimicrobial spectrum of clinical pathogenic fungus

[0030]The antagonistic activity of Bacillus sp.AH-E-1 against various filamentous fungi was determined by simultaneous plate confrontation method. After culturing the fungal spore suspension on 10ml potato agar medium at 28°C for 5 days, take a colony block with a diameter of 7mm, and place it upside down on a sterile potato agar medium plate, so that the mycelium is in contact with the medium, and the colony 2cm away from the side of the dish. After inoculating a Bacillus sp.AH-E-1 strain 2cm away from the side of the dish in the direction facing the colony, culture it at 28°C for 5-7d. The control group was not inoculated with Bacillus. Determination of bacteriostatic degree, bacteriostatic degree=[(fungal colony area of ​​control group−fungal colony area of ​​treatment group) / fungal colony area of ​​control group]×100%. Three repetitions were set up for each treatment.

[0031...

Embodiment 3

[0036] Embodiment 3: the mensuration of phytopathogenic fungi antimicrobial spectrum

[0037] The antagonistic activity of Bacillus sp.AH-E-1 against various filamentous fungi was determined by simultaneous plate confrontation method. After culturing the fungal spore suspension on 10ml potato agar medium at 28°C for 5 days, take a colony block with a diameter of 7mm, and place it upside down on a sterile potato agar medium plate, so that the mycelium is in contact with the medium, and the colony 2cm away from the side of the dish. After inoculating a Bacillus sp.AH-E-1 strain 2cm away from the side of the dish in the direction facing the colony, culture it at 28°C for 5-7d. The control group was not inoculated with Bacillus. Determination of bacteriostatic degree, bacteriostatic degree=[(fungal colony area of ​​control group−fungal colony area of ​​treatment group) / fungal colony area of ​​control group]×100%. Three repetitions were set up for each treatment.

[0038] The i...

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Abstract

The invention relates to a novel wide-spectrum bacterial strain Bacillus sp. AH-E-1 inhibiting clinical and plant pathogenic fungi, and an application thereof. The main content comprises: (1) Bacillus sp. AH-E-1 is a newly-discovered bacillus strain or subspecies; (2) Bacillus sp. AH-E-1 can produce anti-fungus substances after fermentation under appropriate conditions; (3) Bacillus sp. AH-E-1 can significantly inhibit 20 clinical pathogenic fungi such as aspergillus flavus, aspergillus fumigates, trichophyton rubrum, trichophyton mentagrophyte, candida glabrata, cryptococcus neoformans, and the like; (4) Bacillus sp. AH-E-1 can significantly inhibit 13 plant pathogenic fungi such as staphylococcus, magnaporthe oryzae, rhizopusstolonife, and the like. The invention provides an excellent novel anti-fungus substance producing bacterial strain which has the advantages of wide fungus-inhibiting spectrum, obvious effect, and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a novel bacterial strain Bacillus sp.AH-E-1 for broad-spectrum inhibition of clinical and plant pathogenic fungi and its application. More specifically, it relates to a new type of biocontrol bacteria and probiotics with broad-spectrum antifungal functions. Background technique [0002] Human fungal infections generally fall into two categories: superficial fungal infections and deep fungal infections. Superficial fungal infections are usually caused by various dermatophytes, mainly invading the skin, hair, nails, etc.; deep infections are usually caused by Candida albicans, Cryptococcus neoformans, Coccidioides immobilis, etc., mainly invading internal organs and deep tissues , very harmful, often life-threatening. In recent years, with the extensive use of broad-spectrum antibacterial drugs and immunosuppressive drugs, as well as the emergence of cancer chemotherapy, organ transpla...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04C12R1/07
Inventor 金敏李君文陈娜邱志刚谌志强陈照立王新为
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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