442 results about "Rhizopus oryzae" patented technology

The invention relates to the field of microbial fermentation, in particular to a preparation method of a compound Daqu, so as to solve the problems of the traditional Daqu such as single nutritional components of raw materials, longer starter-making and brewing time, low utilization rate of grains, high cost and the like. The preparation method comprises the following steps: (1) treating the raw materials of the Daqu; (2) inoculating aspergillus niger 41254, aspergillus niger UV-11, monascus, mucor racemosus, actinomucor elegans, rhizopus oryzae, saccharomyces cerevisiae, candida drusei, acetobacter, bacillus subtilis and lactobacillus delbrueckii 6100 into a conical flask containing the raw materials of the Daqu for culturing, then expanding the culture and obtaining a mother culture; and(3) adopting uncooked materials for making a starter, inoculating the obtained mother culture and the raw materials of the Daqu into a solid fermentation device, keeping the temperature at 30 DEG C-33 DEG C, continuing for 30-33h, discharging the starter and finally carrying out low-temperature vacuum drying. The preparation method enriches the nutritional components in the raw materials and improves the quality of a brewed product; and a plurality of bacterial strain are artificially added, thereby improving the utilization rate of the grains, shortening the starter-making cycle, improving the yield of the brewed product and reducing the production cost.

The invention relates to a method for producing L-lactic acid. In the method, crop straw and Rhizopus oryzae serve as raw materials. The method particularly comprises the following operation steps: A. preparing straw saccharified liquid; B. adding auxiliary material to inoculate Rhizopus oryzae for fermentation; C repeatedly fermenting 25 batches; D. acidizing fermented clear liquid; E. ultrafiltration; F. nanofilteration; G. reverse osmosis; H. ion exchange; I. concentrating in vacuum to obtain the L-lactic acid product with the mass percentage concentration of 85%. The invention takes the straw hydrolysis saccharified liquid as the main raw material to prepare L-lactic acid, which obviously lowers the production cost of L-lactic acid; Rhizopus oryzae is adopted for once inoculation proliferation, thallus can be reused for several times to produce L-lactic acid, thus shortening L-lactic acid fermentation period, and obviously improving the fermentation strength of L-lactic acid; an advanced membrane separation and ion exchange integration technology is adopted to improve the lactic acid product quality; and the invention has simple process and strong continuity.

The present invention discloses a method for producing chitin / chitosan from the shell of silkworm chrysalis, fly larvina, which comprises: to break into pieces to progress the shell of silkworm chrysalis, fly larvina by dry or wet method; React the fine grits obtained with the fat enzyme and protein enzyme from micro-organism by a enzymolysis reaction in order to to eliminate the fat and protein contained in the chrysalis / larvina raw material; bleach using two-step method; Collect the materials after bleach and to wash, dry materials to obtain chitin product; Obtain chitosan by to circulate to cast off Acetyl reaction in a Rhizopus oryzae full cell immobilization biology reaction vessel; Obtain height hydrosoluble shel oligosaccharide by liquid submerged fermentation chitosan using acid formers. The present invention improves the quality of the product and the efficiency of production; reduces greatly the energy cost, the usage of acid, alkali, oxidant and other harmful chemistry thing and the discharge of effluent, waste residue; produces byproducts of high value at the same time; utilizes the resources integratedly; and increases the benefits of production.

A (Rhizopus Oryzae)ME-F11, its mutation screening method and method for fermenting fumaric acid are disclosed. The preserving code is CCTCC NO: M 207066. The fumaric acid concentration reaches to 90-120g / L, total yield reaches to 90-95 wt% . It's simple and can be used for industrial production.

Fermented soybean foods rich in gamma-aminobutyric acid are produced by fermentation of soybean using Tempe molds. Fermented soybean foods rich in gamma-aminobutyric acid and free amino acids are produced by fermentation of soybean using Tempe molds with anaerobic treatment. Fermented cereal foods rich in gamma-aminobutyric acid and free amino acids are also produced by fermentation of cereals with anaerobic treatment. Rhizopus oligosporus and Rhizopus oryzae are favorably used as the Tempe mold and Koji molds.

The invention discloses a Chinese herb koji formula, wherein the Chinese herb koji formula production raw materials comprise, by weight, 56% of rice, 20% of wheat bran, 10% of rice bran, 0.3% of Q303 rhizopus, 0.2% of rhizopus oryzae, 2% of licorice root, 2.5% of polygonum hydropiper, 1% of xiaosanzaojiao, 2% of oldenlandia cantonensis how, 1% of feikou, 1% of clove, 1% of fennel, 1% of cinnamomum cassia presl, 1% of pericarpium citri reticulatae, and 1% of cortex acanthopanacis. According to the present invention, the formula and the production method of the Chinese herb koji are provided, the advantages of the Xiaoqu, the Bran koji and the traditional Chinese herb particle koji are summarized with the production of the Chinese herb koji of the present invention, the Chinese herb koji of the present invention has the same advantages of convenient use and stable yield as the bran koji and the Xiaoqu during the wine production while the traditional excellent flavor is retained on the wine quality, the disadvantages of various qu are perfectly overcome, and during the production process, the hypha grows uniformly and sturdily, the baking is uniform and fast, the production is simple, the quality is stable, such that the production method is suitable for industrial production.

The invention belongs to the technical field of white-wine brewing processes. In order to solve the problems of low wine production rate and ethyl-acetate / ethyl-lactate rate in summer in the solid fermentation process of the traditional faint-scent white wine, the invention provides a production method for brewing the faint-scent white wine by fermentation of multi-strain enhanced yeasts. The production method comprises the steps of utilizing fresh fermented grains of fen wine to screen wine-brewing yeasts with high ester yield, rhizopus oryzae for producing amylase, acid-resistant and alcohol-resistant monascus fulginosus and high-temperature-resistant bacillus subtilis with the activities of protease and amylase and prepare wine yeast for producing the wine-brewing yeast, bran koji for producing the rhizopus oryzae, rice koji for producing the monascus fulginosus and bacteria solution for producing the bacillus subtilis, then adding into grains together with the yeast for production by a certain proportion, putting into a jar for fermentation and thus obtaining the faint-scent white wine. The production method provided by the invention has the beneficial effects that the cost is reduced; the obtained wine is high in ethyl acetate / ethyl lactate rate, total-acid content and wine production rate and strong in ester fragrance and has faint scent, preserved fragrance and sauce feel, and tastes sourly and coolly with mellowness and softness, and the difficulties of low and uncoordinated wine fragrance, lack of mellowness and short aftertaste due to low ethyl-acetate / ethyl-lactate rate and low acidity of the faint-scent white wine are solved.

The invention relates to a method for preparing a germinated brown rice ferment drink. Germinated brown rice is processed in an Aspergillus oryzae and Rhizopus oryzae mixed culture manner, is decomposed by using the enzyme system complementarity, and is inoculated with lactic acid bacteria to carry out fermentation. The prepared germinated brown rice ferment drink is rich in organic acids, variousfree amino acids, soluble dietary fibers, and active components which are gamma-aminobutyric acid and SOD, and has the advantages of sour-sweet mouthfeel, convenience in drinking, no additives, excellent nutrition and healthcare effects, great improvement of the raw material utilization rate in a two-stage fermentation process, and great solving of resource wastes of existing brown rice having bad mouthfeel.

The invention discloses a microbial compound preparation, a preparation method and a method for treating kitchen garbage. The method for treating kitchen garbage is to separate out mixed bacteria of bacteria and fungi with a certain ratio from the natural world and fermented food, and then prepare the mixed bacteria preparation to effectively treat the kitchen garbage. The preparation comprises the mixed bacteria including various bacteria, actinomycetes, yeast and mycetes. The preparation is characterized in that the mixed bacteria further comprise bacillus subtilis, bacillus licheniformis, high-temperature monad, streptomycete, lactobacillus plantarum, lactobacillus acidophilus, brewer's yeast, pichia pastoris, aroma-producing yeast, rhizopus chinensis, rhizopus oryzae and mucor indicus. The preparation is good for treating the kitchen garbage, non-toxic, harmless, and of genetic stability.

The present invention discloses a preparation method of high-SOD-activity fruit and vegetable enzyme powder. The enzyme powder is prepared by squeezing Chinese watermelon flesh and apple flesh into pulp, respectively fermenting filtrate using saccharomyces cerevisiae and hansenula anomala and streptococcus thermophilus and lactobacillus plantarum, fermenting filter residues with rhizopus oryzae suspension, mixing the three fermentation liquor, conducting secondary fermentation on the mixture, and finally spray-drying the mixture. The prepared enzyme powder is good in mouthfeel and flavor, high in SOD activity and high in lactic acid bacteria content.

The invention discloses a fluorescence quantitative PCR primer, a probe and a kit for detecting ordinary pathogenic fungi. A specific primer and a TaqMan probe are independently designed, and a fluorescence PCR detection method which can be used for simultaneously detecting 15 clinically ordinary pathogenic fungi, including 8 candida mycoderma bacteria (candida albicans, candida glabrata, candida parapsilosis, candida kefyr, candida sake, candida kruse, candida guilliermind and candida tropicalis), four aspergilli (aspergillus niger, aspergillus flavus, aspergillus terreus and aspergillus fumigates), cryptococcus, rhizopus oryzae and mucor circinelloides, is established. The method is relatively high in sensitivity and specificity, and has significant meanings for early diagnosis and treatment on invasive infections with fungi.

The invention provides a fermentation technology of low-salt fermented bean curd. The fermentation technology comprises the following steps: base preparing, inoculating and cultivating, spraying, pre-fermenting, hair-rubbing and pickling, and soup-filling. According to the technology, the bases are prepared by steam-boiling of soybean slurry; a mixed spore suspension of hair mould and Rhizopus oryzae is utilized in grafting; table salt is used for hair-rubbing and curing of the bean-curd white bases, wherein the weight of the table salt is 10-14% that of the bean-curd white bases; and alcohol with the alcoholicity being 16-20% is used for soup-filling. The fermented bean curd is prepared under multi-aspect coordinated actions, so that the prepared final products are small in individual difference and batch difference. The prepared low-salt fermented bean curd is good in appearance and taste and is not prone to deterioration.

The invention discloses a method for treating heavy metal wastewater. The method comprises the following steps: (1) immobilizing mixed bacteria of pseudomonas pseudoalcaligenes and saccharomyces cerevisiae, collected after mixed fermentation culture, into immobilized cell spheres, wherein the appearances of the immobilized cell spheres are spherical, and the interiors of the immobilized cell spheres are of a porous mesh structure; (2) adding a rhizopus oryzae spore suspension liquid into starch bearing material hydrolysate as a culture medium at the pH of 3-6, carrying out shake cultivation for 24-48 hours, filtering to collect rhizopus oryzae mycelium spheres with the diameters of 50-100 microns; (3) filling a biological adsorber with the immobilized cell spheres and the rhizopus oryzae mycelium spheres, wherein the biological adsorber is formed by virtue of series connection of multiple adsorption columns; and (4) after regulating the initial heavy metal ion concentration of heavy metal wastewater to be less than or equal to 800mg / L and the pH value to be 5-7, enabling heavy metal wastewater to flow through the biological adsorber, wherein the hydraulic retention time in each adsorption column is 0.5h-1h. According to the method, different heavy metals (Cd<2+>, Hg<2+>, Cu<2+>, Zn<2+>, Ni<2+>, Ag<2+> and Pb<2+>) can be well adsorbed by virtue of different types of microbial live bacteria under different adsorption mechanisms.

The invention relates to a composite microbial deodorant for treating household garbage and a preparation method of the deodorant. The composite microbial deodorant is prepared from raw materials as follows: 20-30 parts of composite photosynthetic bacterium powder, 10-20 parts of Bacillus subtilis powder, 20-40 parts of composite aspergillus powder and 30-50 parts of composite yeast powder. According to the composite microbial deodorant, hydrogen sulfide, ammonia as well as strains adapted to the household garbage are screened and degraded in a targeted manner, the composite microbial deodorant comprises beneficial microbes such as Thiocapsa roseopersicina capable of efficiently degrading the hydrogen sulfide, Rhodospirillum rubrum capable of efficiently degrading fatty acid series odors and the ammonia, Aspergillus oryzae rich in enzyme systems such as proteases, cellulose and the like, Bacillus subtilis capable of quickly degrading the ammonia, Rhizopus oryzae rich in lactic acid, Saccharomyces cerevisiae for producing ethyl alcohol, Candida guilliermondii rich in aroma-producing substances and the like, the beneficial microbes grow and breed in the household garbage and have the synergistic effect, the ecological environment of the household garbage is effectively improved, and emissions of the hydrogen sulfide, the ammonia and the fatty acid series odors are greatly decreased.

The invention discloses a biological organic fertilizer with an insect expelling function and a preparation method thereof. The fertilizer comprises the following components: tobacco waste, 1 to 2 percent of calcium-magnesium phosphate, 1 to 2 percent of ammonium sulfate, 3 to 5 percent of waste molasses and 0.3 to 0.5 percent of zymocyte based on the total weight of the tobacco waste; and the zymocyte comprises 20 to 35 weight percent of trichoderma viride, 20 to 35 weight percent of bacillus subtilis, 10 to 20 weight percent of rhizopus oryzae, 10 to 20 weight percent of chaetomium and 10 to 20 weight percent of thermomonospora fusca. The preparation method comprises the following steps of: pretreating the tobacco waste; smashing tobacco waste raw materials into sawdust-like grains of 40 to 60 meshes; then adjusting the carbon-to-nitrogen ratio of the raw materials to 20 to 30:1 with grass peat or straw powder; and then adjusting the pH value to 6.5 to 7.5 with Ga(OH)2 and the water content to 40 to 50 weight percent. Due to the adoption of the biological organic fertilizer with the insect expelling function, nicotine in the raw materials can be preserved to the greatest extent, insect damage degree is lowered, the using amount and times of agricultural chemicals are reduced, the application of the agricultural chemicals is effectively reduced, and the production is environmentally friendly.

The invention provides a method for preparing a feed from common andrographis herb dregs by virtue of fermentation. The method comprises the following steps: (1) pretreatment of dregs, namely drying the dregs until the moisture content is 2-5wt% and grinding until the power granularity is within the range of 2.5-10 meshes; (2) preparation of a dreg culture medium, namely mixing 50 parts of dregs with bran in the ratio of 1: 1 to 2: 1, 0.2-0.6 part of corn flour, 0.2-0.6 part of ammonium chloride and 0.1-0.3 part of monopotassium phosphate in 25-50 parts of sterile water, pouring the obtained mixed liquid into a conical flask and mixing evenly to obtain natural pH, and sterilizing at 121 DEG C under high pressure for 20 minutes; (3) dreg fermentation, namely inoculating 10-30mL of the second-level seeds of rhizopus oryzae strain or bacillus subtilis strain into each 100g of dreg culture medium, fermenting at 26-30 DEG C for 48-96 hours, and shaking the culture medium evenly every 12 hours; (4) product treatment, namely drying the culture medium obtained by fermentation, and grinding and sieving by use of a sieve of 2-4 meshes, thereby obtaining the fermented feed product. The method is simple in process, short in time, low in material consumption, advantageous for industrial pilot scale-up, and relatively high in yield.

The invention relates to a method of D-pantolactone, in particular to a method taking chemically synthesized DL-pantolactone as a substrate for preparing the D-pantolactone by the mixed fermentation of microorganisms. The method adopts Aspergillus terrus DH-069-CGMCCNo.2498 and Rhizopus oryzae QY-022-CGMCCNo.2497 which are microbial flora for producing the hydrolase of the D-pantolactone as enzyme-producing strains for mixed fermentation. The method has the advantages that: the hydrolase of the D-pantolactone produced under the coordination of two strains is utilized to separate the DL-pantolactone, time for producing enzyme and fermentation is short (two days), enzymatic conversion time is also short (3 hours to 30 hours) and the enzyme can be reused for more than 8 times; one-time conversion rate of the enzyme is 38 percent to 56 percent; the total conversion of the substrate is 85 percent to 95 percent; the extraction yield of products is more than 60 percent; the major quality indexes of the D-pantolactone are as follows: specific rotation (Alpha)D<20> is minus 48 degrees to minus 51 degrees and optical purity is 99 percent e.e.

The invention belongs to the technical field of biological engineering, which relates to a method for separating and extracting boletic acid in situ in the process of utilizing rhizopus oryzae fermentation to prepare boletic acid. The invention aims at the special physicochemical properties of boletic acid, when rhizopus oryzae free cell is utilized to ferment, the invention adopts a hollow fiber ultrafilter membrane, concentration by nanofiltration and ion exchange adsorption coupling technique to separate and extract boletic acid. The operation condition of the method is mild, separation selectivity is strong, activity loss of somatic cell is small, fermentation efficiency of rhizopus oryzae is effectively improved, process flow of utilizing a biological method to synthesize boletic acid is reduced, and meanwhile, the invention utilizes a nanofiltration device to concentrate fermentation liquor in the process of reacting and separating, which greatly improves adsorption capacity of ion exchange resin to boletic acid, and material consumption and energy consumption are relatively lower, and waste and secondary pollutants which are produced are relative little. The reacting and separating process of the method are fast, complete, and operation stability is excellent, the method is a high-effective and novel mass transfer separation coupling strengthening technique, which has wider industrialized production prospect.

The invention relates to a high-yield 'cellulose, hemicelluloses and lignin enzyme' straw decomposition additive and a preparation method thereof. The high-yield 'cellulose, hemicelluloses and ligninenzyme' straw decomposition additive comprises the following preparation raw materials: a cellulose, hemicelluloses and lignin enzyme mixture, plant materials, sugar and urea, wherein the cellulose, hemicelluloses and lignin enzyme mixture consists of cellulase bacillus subtilis fermentation liquor, a rhizopus oryzae fermentation substance and ligninase white-rot fungi fermentation liquor; the weight ratio of the cellulase bacillus subtilis fermentation liquor to the rhizopus oryzae fermentation substance to the ligninase white-rot fungi fermentation liquor is 3-7 to 5-10 to 5-10. By adoptingthe high-yield 'cellulose, hemicelluloses and lignin enzyme' straw decomposition additive provided by the invention, different bacterial florae can harmoniously coexist and be mutually promoted, an effect of complementary advantages can be achieved, the effective viable count can be increased, the enzyme yield can be increased, cellulose, hemicelluloses and lignin in crop straw can be rapidly andeffectively decomposed, and rapid treatment and circulation of straw biomass resources can be achieved.

The invention discloses a Saccharomyces cerevisiae genetic engineering strain for producing fumaric acid as well as a construction method and application thereof, belonging to the field of genetic engineering. The construction method provided by the invention comprises the following steps: cloning key genes containing dehydrogenase genes (RoMDH) and fumarase genes (RoFUM1) in the accumulation path of the fumaric acid from the Rhizopus oryzae NRRL1526 capable of accumulating a large amount of fumaric acid, and converting the Saccharomyces cerevisiae BMA64 by connecting to the plasmid pY26TEF / GPD capable of realizing high-copy two-way expression in the Saccharomyces cerevisiae to obtain the Saccharomyces cerevisiae genetic engineering strain for producing fumaric acid. The Saccharomyces cerevisiae genetic engineering strain disclosed by the invention can accumulate the fumaric acid in the cytoplasm, the fermentation method is simple, the yield can reach 26.2mg / L, and the invention establishes a basis for industrialization of Saccharomyces cerevisiae for producing fumaric acid in metabolic engineering construction.

The invention discloses special fodder for the goose fattening stage. The special fodder comprises, by weight, 10-20 parts of corn, 20-30 parts of wheat, 15-30 parts of rice bran, 0.5-2 parts of lard oil, 2-5 parts of sweet potato leaves, 1-2 parts of grape seed oil, 10-30 parts of corn protein powder, 1-2 parts of fish meal, 1-3 parts of soybean protein concentrate, 5-10 parts of chrysanthemum dregs, 5-15 parts of dried blood, 2-6 parts of fermentation biological protein, 0.5-1.5 parts of feather meal, 2-5 parts of cotton dregs, 5-15 parts of maize germ pulp, 0.5-2 parts of rock flour, 1-5 parts of se-enriched yeast, 2-6 parts of Chinese herbal medicine additives, 0.2-0.8 part of compound enzyme, 0.2-0.8 part of phytase, 0.01-0.04 part of white ginseng, 0.1-0.5 part of armillaria mellea, 0.01-0.05 part of rhizopus oryzae, 0.02-0.08 part of lysine and 0.02-0.09 part of methionine. The special fodder is green, healthy, balanced in nutrition, remarkable in weight put-on effect and low in cost.

The invention relates to a high-efficiency engineering bacteria agent and a method for producing an active biological organic fertilizer and belongs to the technical field of biology. The bacteria agent consists of the following strains in parts by weight: 50 to 60 parts of enzyme producing bacteria, 6 to 10 parts of bacillus thuringiensis and 10 to 15 parts of bacillus subtilis, the enzyme producing bacteria consist of the following strains in parts by weight: 1 to 3 parts of umbrella mucor, 1 to 3 parts of rhizopus oryzae, 3 to 5 parts of bacillus licheniformis and 3 to 5 parts of Pichia pastoris. The high-efficiency engineering bacteria agent provided by the invention can realize high-efficiency conversion of harmlessly treated kitchen waste to obtain the high-efficiency active biological organic fertilizer. According to determination, the organic fertilizer satisfies relevant indicators of organic fertilizers in agricultural standard NT 525-2012 on organic fertilizers, can effectively increase the number of soil organic matters and effective microbial communities, can activate soil, can promote the absorption of plant root systems to nutrients, can improve the yield and quality of crops, and is a high-grade fertilizer for producing pollution-free and organic agricultural products.

The invention discloses a method for manufacturing cheese flavor milk essence bases. The method includes steps of fermenting whole-milk powder by the aid of lactic acid bacteria and rhizopus oryzae to obtain milk fermented broth; adding milk fat into the milk fermented broth and carrying out enzymatic hydrolysis on the milk fermented broth by the aid of lipase and protease to obtain milk enzymatic hydrolysis liquid; adding amino acid, reducing sugar and solvents into the milk enzymatic hydrolysis liquid, and enabling the amino acid, the reducing sugar, the solvents and the milk enzymatic hydrolysis liquid to carry out thermal reaction to obtain the cheese flavor milk essence bases. The method has the advantages that procedures for manufacturing the cheese flavor milk essence bases are natural, generated aroma is natural, harmonious and mellow, the cheese flavor milk essence bases have certain heat resistance and can be applied to milk beverage, baking food and home cooking food, and accordingly the method has a broad market prospect.

Production cellulase by microbe mixed fermentation is carried out by taking Trichoderma koningii and Rhizopus oryzae as cellulase production bacterium, inoculating spore fluid or secondary seed fluid into solid or liquefied culture medium and controlling culture medium and amount of Trichoderma koningii and Rhizopus oryzae and other culture conditions during mixed bacterium culture. The filter-paper activity, beta-glucosidese activity are increased by 60-100% and 400-500% and fermentation time is shortened. It's benefit to secrete cellulase and improves enzyme system. It's cheap and simple.

The invention relates to a method for preparing and regenerating a rhizopus oryzae protoplast for producing L-lactic acid at high yield, comprising the following steps of: A. preparing a rhizopus oryzae spore suspension; B. preparing a seed culture liquid; C. preparing a hypha suspension; D. enzymolyzing a cell wall; E. separating a protoplast; and F. regenerating the protoplast. The rhizopus oryzae protoplast prepared with the method disclosed by the invention is in an approximately-circular shape and has higher activity; the preparation quantity and the regeneration rate of the rhizopus oryzae protoplast can respectively reach 2.74*10<6> / mL and 6.8 percent to the maximum extent. The method is easy to operate, has low requirements for the equipment, and provides a technical reference for preparing and regenerating the rhizopus oryzae protoplast. By using the method disclosed by the invention, the protoplast can be quickly prepared with short regeneration period and high regeneration speed; and the preparation efficiency of the protoplast can be effectively improved. The protoplast prepared with the method provided by the invention completely meets the requirement for fusing the protoplast. The invention provides an operation method with practical significance for preparing and regenerating the protoplast.

Provided is a Rhizopus oryzae TY GF1 bacterial strain. The preservation date is December 20th in 2010 and the preservation registration number is CGMCC No.4490. The application of the Rhizopus oryzae TY GF1 bacterial strain in degrading high-concentration oil in catering waste water effectively is further provided.

The invention belongs to the field of food fermentation, and in particular relates to rhizopus for food fermentation and application. Rhizopus oryzae zsm-003, zsm-004 and rhizopus zsm-005 suitable for rice product and sweet rice wine fermentation are separated from sweet rice wine. A microorganism complex bacterium agent for food fermentation is developed, which comprises rhizopus oryzae zsm-003, zsm-004 and brettanomyces custersii ZSM-001. Strain survival rate of the complex fermenting agent reaches more than 35%, and the produced sweet rice wine is relatively rich in sugar composition, intense in flavor, good in taste, and relatively high in contents of delicious amino acid and essential amino acid. Furthermore, the invention discloses a preparation method and application of the complex fermenting agent.

The invention belongs to the technical field of energy and relates to a method for producing biodiesel by microalgae fermentation. The method comprises the following steps of 1, microalgae culture, 2, fermentation of the cultured microalgae by trichoderma harzianum and rhizopus oryzae as zymophyte and a fermentation culture medium containing 20-30g / L of microalgae, 3-5g / L of sodium citrate, 1.5-2.5g / L of o-toluidine, 10-15g / L of sodium chloride, 0.5-1g / L of potassium dihydrogen phosphate and 3-5g / L of magnesium sulfate, 3, extraction of grease in the fermentation products, and 4, enzymic catalytic reaction of the grease. The biodiesel obtained by the microalgae fermentation has a fast oil production rate and a low production cost.

The invention discloses Bacillus velezensis 05-1205 as well as an acquisition method and application thereof, and belongs to the technical field of microbial application. The Bacillus velezensis 05-1205 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the Bacillus velezensis 05-1205 is CCTCC No: M2020568. The acquisition method comprises processes of separating, screening and purifying, firstly, endophytic bacteria are seperated from healthy tobacco plants, endophytic bacteria antagonizing rhizopus oryzae are screened by taking the tobacco mildew pathogenic bacteria rhizopus oryzae in a baking period as an indicator, and finally, purifying is conducted on a culture medium plate to obtain the Bacillus velezensis 05-1205. The application ofthe bacterial strain comprises application in tobacco mildew control, the bacterial strain Bacillus velezensis 05-1205 can achieve effective control over tobacco mildew in the baking period, and therelative control effect is 70% or above. Secondly, the Bacillus velezensis 05-1205 is wide in antibacterial spectrum, and has a very good antibacterial effect on tobacco bacterial wilt bacteria, botrytis cinerea, sclerotinia sclerotiorum, target leaf spot bacteria, root rot bacteria and alternaria alternata besides pathogenic bacteria of tobacco mildew in the baking period.

A process for producing an oil-and-fat having a ratio between a 1,3-disaturated long-chain fatty acid 2-monounsaturated long-chain fatty acid triglyceride (SUS) and a 1,2-disaturated long-chain fatty acid 3-monounsaturated long-chain fatty acid triglyceride (SSU) of 9 / 1 or greater by mass, which comprises conducting the transesterification by reacting an oil-and-fat containing the SUS and the SSU and having the SUS content of 50% by mass or more with a lipase selected from the group consisting of a lipase derived from a microorganism belonging to the genus Thermomyces sp., a lipase derived from Rhizopus oryzae and a lipase derived from Rhizopus delemar which are 1,3-selective lipases. The process is suitable for the production of a hard butter having excellent properties for use as a cocoa butter equivalent (CBE) or a cocoa butter improver (CBI) on an industrial scale.