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Method for treating heavy metal wastewater

A technology for heavy metals and wastewater, applied in biological treatment devices, chemical instruments and methods, biological water/sewage treatment, etc., to achieve the effects of improving water-soluble swelling, increasing tolerance, and low price

Active Publication Date: 2016-07-13
梅州市华禹污水处理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, most of the current literatures only use a single type of microorganisms as biosorbents, or mix deactivated microorganisms with common adsorbents, such as activated carbon, humic acid, sepiolite, polysaccharide resin, etc.

Method used

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  • Method for treating heavy metal wastewater
  • Method for treating heavy metal wastewater

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Experimental program
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Embodiment 1

[0027] The mixed fermentation culture process of embodiment 1 class Pseudomonas alcaligenes and Saccharomyces cerevisiae comprises the following steps:

[0028] 1) Cultivation of Saccharomyces cerevisiae seed solution

[0029] Saccharomyces cerevisiae slant strains were inoculated on the first-level seed medium for shake flask culture. After culturing for 24 hours at 27-30°C with a rotation speed of 260rmp, they were inoculated in the second-level seed medium for fermentation tank cultivation at 30°C with a rotation speed of 260rmp and aeration Amount of 2.5 ~ 3m 3 After 24 hours of cultivation per hour, the seed liquid of Saccharomyces cerevisiae was obtained, wherein the primary seed medium was: yeast extract 5g / L, peptone 6g / L, glucose 20g / L; the secondary seed medium was: glucose 40g / L , yeast extract 5g / L, peptone 5g / L, (NH4)2SO42g / L, MgSO4 7H2O0.25g / L, NaCl0.15g / L, KH2PO42.8g / L.

[0030] 2) The cultivation of Pseudomonas alcaligenes seed solution

[0031] Inoculate th...

Embodiment 2

[0034] Embodiment 2 A preparation method of immobilized cell spheres, comprising the following steps:

[0035] A) After mixing polyvinyl alcohol, calcium alginate, acryloyl and water at a mass ratio of 12:1:4:100, polyvinyl alcohol hydrosol is obtained, and the alkali-like pseudomonas collected after mixed fermentation culture Bacteria and Saccharomyces cerevisiae cells were added to polyvinyl alcohol hydrosol to form a mixed solution, wherein the addition amount of Pseudomonas alcaligenes and Saccharomyces cerevisiae cells met the product of adding 5g wet weight per 100mL of polyvinyl alcohol hydrosol. Alkaline Pseudomonas and Saccharomyces cerevisiae mixed bacteria; B) Add the mixed solution to the solution containing 4w / v% N'-methylene bisacrylamide and 1.5w / v% CaSO with a peristaltic pump 4 In a saturated boric acid solution, gel balls are formed, placed at -20°C for 5-10 hours, thawed at room temperature, and then cooled at -10°C for 1-2 hours, after thawing at room tempe...

Embodiment 3

[0036] Embodiment 3 A preparation method of immobilized cell spheres, comprising the following steps:

[0037] A) After mixing polyvinyl alcohol, calcium alginate, acryloyl and water at a mass ratio of 6:0.5:3:100, polyvinyl alcohol hydrosol is obtained, and the alkali-like pseudomonas collected after mixed fermentation culture Bacteria and Saccharomyces cerevisiae cells were added to polyvinyl alcohol hydrosol to form a mixed solution, and the addition amount of Pseudomonas alcaligenes and Saccharomyces cerevisiae cells was controlled to meet the production of 4g wet weight per 100mL of polyvinyl alcohol hydrosol. Alkali Pseudomonas and Saccharomyces cerevisiae mixed bacteria; then the mixed solution was added to the solution containing 3w / v% N'-methylene bisacrylamide and 1w / v% CaSO with a peristaltic pump 4 In a saturated boric acid solution, gel balls are formed, placed at -20°C for 5-10 hours, thawed at room temperature, and then cooled at -10°C for 1-2 hours, after thawi...

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Abstract

The invention discloses a method for treating heavy metal wastewater. The method comprises the following steps: (1) immobilizing mixed bacteria of pseudomonas pseudoalcaligenes and saccharomyces cerevisiae, collected after mixed fermentation culture, into immobilized cell spheres, wherein the appearances of the immobilized cell spheres are spherical, and the interiors of the immobilized cell spheres are of a porous mesh structure; (2) adding a rhizopus oryzae spore suspension liquid into starch bearing material hydrolysate as a culture medium at the pH of 3-6, carrying out shake cultivation for 24-48 hours, filtering to collect rhizopus oryzae mycelium spheres with the diameters of 50-100 microns; (3) filling a biological adsorber with the immobilized cell spheres and the rhizopus oryzae mycelium spheres, wherein the biological adsorber is formed by virtue of series connection of multiple adsorption columns; and (4) after regulating the initial heavy metal ion concentration of heavy metal wastewater to be less than or equal to 800mg / L and the pH value to be 5-7, enabling heavy metal wastewater to flow through the biological adsorber, wherein the hydraulic retention time in each adsorption column is 0.5h-1h. According to the method, different heavy metals (Cd<2+>, Hg<2+>, Cu<2+>, Zn<2+>, Ni<2+>, Ag<2+> and Pb<2+>) can be well adsorbed by virtue of different types of microbial live bacteria under different adsorption mechanisms.

Description

technical field [0001] The invention relates to the field of sewage treatment, in particular to a method for treating heavy metal wastewater. Background technique [0002] At present, there are three main types of heavy metal wastewater treatment methods: chemical reaction method, concentration separation method and biological adsorption method. The first category is the removal of heavy metal ions in wastewater through chemical reactions, including neutralization precipitation, sulfide precipitation, ferrite co-precipitation, chemical reduction, electrochemical reduction, and polymer heavy metal collectors; The second category is the method of concentrating and separating heavy metals in wastewater without changing their chemical form, including adsorbent adsorption, ion exchange and membrane separation; the third category of biosorption is a new development this year. The heavy metal sewage treatment method uses microorganisms and their derivatives to absorb heavy metals ...

Claims

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Application Information

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IPC IPC(8): C02F3/34B01J20/26B01J20/30C02F101/20
CPCB01J20/223B01J20/24B01J20/261B01J20/262B01J20/264C02F3/34C02F3/347C02F2101/20C02F2203/006C12N1/14C12N1/16C12N1/18C12N1/20C12N11/04C12N11/08C12N11/10
Inventor 洪寅
Owner 梅州市华禹污水处理有限公司
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