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70 results about "Pseudomonas alcaligenes" patented technology

Pseudomonas alcaligenes is a Gram-negative aerobic bacterium used for bioremediation purposes of oil pollution, pesticide substances, and certain chemical substances, as it can degrade polycyclic aromatic hydrocarbons]]. It can be a human pathogen, but occurrences are very rare. Based on 16S rRNA analysis, P. alcaligenes has been placed in the P. aeruginosa group.

A set of living body microorganism preparations for preparing composite microorganism fertilizer and preparation method thereof

The invention relates to a group of alive microbial preparations for preparing compound microbial fertilizer and the preparation method thereof. The alive microbial preparations include a microbial inoculant and an organic material degradation agent. The microbial inoculant includes Bacillus megaterium, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus laterosporus, Azotobocter chroococcum, etc. The organic material degradation agent includes Bacillus megaterium, Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, Saccharomyces cerevisiae, Candida lipolytica, Bacillus pumilus, Bacillus cereus, Streptomyces griseus, Pseudomonas alcaligenes, Pseudomonas cepacia, Streptococcus salivarius subsp. Thermophilus, Geobacillus stearothermophilus, Streptomyces Thermophilus, Geotrichum candidum and Aspergillus oryzae. The preparation method comprises the following steps: carrying out submerged fermentation, directly mixing the bacterial liquids and stirring to obtain a liquid preparation; or mixing with an absorbent to obtain a solid preparation. The alive microbial preparations have the advantages of improving soil fertility, promoting plant growth, enhancing anti-disease, anti-insect and anti-drought capacities of plants, improving quality of agricultural products and so on.
Owner:SHANGHAI SIJI BIOLOGICAL TECH

Oil field re-injection water microbiological treatment

The improved process of treating oil field effluent for reinjection features aerobic biochemical treatment and subsequent filtering. The microbes for the aerobic biochemical treatment include Bacillus cereus, Corynebacterium, Bacillus subtilis and Pseudomonas alcaligenes, and the inflow water has pH value controlled in 6-9. The process can reach oil field water injection standard, including oil not more than 2 mg / l, SS not more than 1 mg / l and grain size mid value not greater than 1 micron. The present invention has the advantages of simple technological process, low cost, stable treating effect, no secondary pollution, etc., and is especially suitable for use in oil field.
Owner:JIANGSU BODA ENVIRONMENTAL PROTECTION

Method for treating heavy metal wastewater

The invention discloses a method for treating heavy metal wastewater. The method comprises the following steps: (1) immobilizing mixed bacteria of pseudomonas pseudoalcaligenes and saccharomyces cerevisiae, collected after mixed fermentation culture, into immobilized cell spheres, wherein the appearances of the immobilized cell spheres are spherical, and the interiors of the immobilized cell spheres are of a porous mesh structure; (2) adding a rhizopus oryzae spore suspension liquid into starch bearing material hydrolysate as a culture medium at the pH of 3-6, carrying out shake cultivation for 24-48 hours, filtering to collect rhizopus oryzae mycelium spheres with the diameters of 50-100 microns; (3) filling a biological adsorber with the immobilized cell spheres and the rhizopus oryzae mycelium spheres, wherein the biological adsorber is formed by virtue of series connection of multiple adsorption columns; and (4) after regulating the initial heavy metal ion concentration of heavy metal wastewater to be less than or equal to 800mg / L and the pH value to be 5-7, enabling heavy metal wastewater to flow through the biological adsorber, wherein the hydraulic retention time in each adsorption column is 0.5h-1h. According to the method, different heavy metals (Cd<2+>, Hg<2+>, Cu<2+>, Zn<2+>, Ni<2+>, Ag<2+> and Pb<2+>) can be well adsorbed by virtue of different types of microbial live bacteria under different adsorption mechanisms.
Owner:梅州市华禹污水处理有限公司

Denitrifying phosphate-accumulating organism with nitrogen and phosphorus removal functions and applications thereof

ActiveCN103114062AEfficient nitrogen and phosphorus removal activityThorough denitrificationBacteriaMicroorganism based processesTherapeutic effectNitrogen gas
The invention relates to a denitrifying phosphate-accumulating organism with nitrogen and phosphorus removal functions and applications thereof. The strain is named as pseudomonas pseudoalcaligenes CL-1, belongs to pseudomonas pseudoalcaligenes, and is preserved in China Center for Type Culture Collection on June 13, 2012, and the preservation numbers is CCTCC NO: M2012225. The strain is gram-positive and belongs to brevibacterium, a bacterial colony is rounded, milk white, convex and glossy, and edges are smooth and transparent. The growth cycle of the organism is long, the nitrogen and phosphorus removal rates in the first 24h are respectively 56.93% and 43.56%, and the nitrogen and phosphorus removal rates in the whole growth cycle are respectively 75.6% and 57.5%. The strain has a complete denitrifying capacity, and can directly reduce inorganic nitrogen such as nitrate nitrogen and nitrite nitrogen and the like to harmless nitrogen gas discharged from water bodies. When the denitrifying phosphate-accumulating organism is used for treating nitrogen and phosphorus containing wastewater, the process is simple, the treatment effect is efficient and stable, and the operating cost is saved, meanwhile, no greenhouse gas such as NO and N2O and the like is produced, therefore, no air pollution is caused.
Owner:SHANDONG UNIV

Waste water treatment agent and application thereof

The invention discloses a waste water treatment agent. The waste water treatment agent is prepared by using the following steps: 1, mixed fermenting, culturing and collecting pseudomonas pseudoalcaligenes and saccharomyces cerevisiae thalli; 2, mixing polyvinyl alcohol, calcium alginate, acrylyl and water according to a mass ratio of (6-15):(0.5-1.5):(3-5):100 to obtain a polyvinyl alcohol hydrosol, and adding the collected pseudomonas pseudoalcaligenes and saccharomyces cerevisiae thalli into the polyvinyl alcohol hydrosol to form a mixed solution; 3, adding the mixed solution into a saturated boric acid solution containing N'-methylene bisacrylamide CaSO4 by using a peristaltic pump to form a gel bead, cooling and unfreezing the gel bead, then cooling and unfreezing the gel bead again, placing in phosphate with the mass concentration of 0.2-0.5 g / L to obtain an immobilized cell bead with a spherical appearance and an internal porous mesh structure. The invention also discloses a method for treating waste water by using the treatment agent. Different heavy metals are well adsorbed.
Owner:湛江市粤绿环保科技有限公司

Hyposmosis/super-hyposmosis oil extraction sewage reinjection method in oil field

The invention relates to an improved hyposmosis / super-hyposmosis oil extraction sewage reinjection method in an oil field, which is characterized in that a combination strain of Zoogloea sp, Bacillus cereus and Pseudomonas alcaligenes mainly for removing oil and a combination strain of Brachybacteriumcollins Bacillus licheniformis Bacillus subtilis and Pseudomons sp for mainly degrading organic pollutants are respectively attached in an aerobic pool, and suspension filling with density of 65-85V / V% and / or separatly arranged suspension filling and biological aerated filter filling are filled and treated in sequence, wherein a separation membrane is a fiber membrane or vacuum membrane or gas stripping membrane or vibration membrane meeting the requirement on filtration, and the fine filtration is diatomite filtration. The synergistic effect of the process greatly improves the preprocessing effect to ensure that the follow-up membrane separation can adopt conventional economic separation treatment such as the hollow fiber membrane or vacuum membrane or gas stripping membrane or vibration membrane, or adopt the diatomite filtration. Compared with the prior art, the invention has the advantages of short treatment period and low investment and treatment cost.
Owner:JIANGSU BODA ENVIRONMENTAL PROTECTION

Complex microbial agent for stabilizing treatment of stock garbage in cities and towns as well as preparation method and application of complex microbial agent

ActiveCN109852557APromote degradationAltered ion valence diseaseFungiBacteriaMicrobial agentPseudomonas putida
The invention provides a complex microbial agent for stabilizing treatment of stock garbage in cities and towns as well as a preparation method and an application of the complex microbial agent. The complex microbial agent contains sporotrichum thermophile, pseudomonas alcaligenes, halobacillus sp, bacillus subtilis, pseudomonas putida, candida utilis, saccharomyces cerevisiae, phanerochaete chrysosporium and lactobacillus plantarum. Growth metabolism, degradability and activity of all microbial strains in the complex microbial agent can effectively complement and cooperate with one another, the microbial strains can grow and propagate quickly in a landfill system to promote the temperature of a garbage treatment environment to increase quickly, meanwhile, organic matter, BDM and toxic andharmful substances in a landfill can be quickly and efficiently degraded, the stabilizing time of the garbage landfill is greatly shortened, stable harmless treatment of landfill is promoted, and thecomplex microbial agent can be applied to aerobic stabilizing treatment of garbage in cities and towns in practice.
Owner:BEIJING GUOHUAN TSINGHUA ENVIRONMENT ENG DESIGN & RES INST CO LTD BEIJING CHINA

Organophosphorus degradating enzyme and coding gene

The invention provides a bacteria strain having a high efficiency broad spectrum degradation ability for an organophosphorus pesticide, the strain belongs to Pseudomonas pseudoalcaligenes, and is named as C2-1, the invention also provides a seperation process, cultivation conditions, and a degradation characteristic of the strain, the strain can be grew on an inorganic salt culture medium by using the pesticide. Also provided is seperation and purification of an organophosphorus pesticide degradation enzyme excreted by the strain, and study on enzyme properties, and seperation and clone of genes coding the enzyme. The invention provides a good gene source for cloning organophosphorus pesticide degradation enzyme genes by using a gene project means, finally realizes an object of producing the organophosphorus pesticide degradation enzyme.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI +1

Compound microorganism microsphere and preparation method thereof

The invention discloses a compound microorganism microsphere and a preparation method thereof, and belongs to the technical field of microorganisms in sewage treatment. The method comprises the following steps that (1) pseudomonas mendocina and pseudomonas pseudoalcaligenes are subjected to primary embedding and crosslinked immobilization; (2) the product obtained through primary crosslinked immobilization in the first step and bacillus subtilis are mixed and are then subjected to secondary embedding and crosslinked immobilization; the compound microorganism microsphere is obtained. Two layers of microorganisms are embedded in the compound microorganism microsphere prepared by the preparation method; the inner layer contains the pseudomonas mendocina and the pseudomonas pseudoalcaligenes; the outer layer contains the bacillus subtilis. The embedding method is used twice, so that the microorganism immobilization is firmer; the bacteria density is increased; the sewage treatment efficiency is improved; the magnet powder has strong adsorption force; the crosslinking effect among mixed microorganisms is enhanced; the immobility of mixed bacteria is improved.
Owner:河南永泽环境科技有限公司

Mixed bacterial colony microbial preparation and application thereof in treatment of wastewater containing nitrate nitrogen

The invention discloses a mixed bacterial colony microbial preparation which is a bacterial suspension consisting of paenibacillus, pseudomonas stutzeri, pseudomonas pseudoalcaligenes and pseudomonas oleovoran, wherein the viable count of paenibacillus is more than 1.69*10<10>CFU / Ml, the viable count of pseudomonas stutzeri is more than 2.35*10<10>CFU / Ml, the viable count of pseudomonas pseudoalcaligenes is more than 2.18*10<10>CFU / Ml, and the viable count of pseudomonas oleovoran is more than 2.26*10<10>CFU / Ml. The mixed bacterial colony microbial preparation can be used for treating wastewater containing nitrate nitrogen, and the specific application method comprises the steps of regulating the pH value of the wastewater containing nitrate nitrogen to 7.0-7.2, then inoculating the wastewater containing nitrate nitrogen with the mixed bacterial colony microbial preparation according to the inoculation amount of 1-10%, and performing static culture at 30-35 DEG C. The denitrification effect can be obvious after 4 days culture and complete denitrification can be realized.
Owner:SHANDONG UNIV

Kitchen garbage biological fermentation microbial inoculum and application thereof

The invention relates to kitchen garbage biological fermentation microbial inoculum. The kitchen garbage biological fermentation microbial inoculum consists of four or more of bacillus subtilis, bacillus licheniformis, cellulomonas flavigena, pseudomonas pesodoalcaligenes, rhodop seudanonas palustris, frankiaceae actinomycesbovis, debaryomyces hansenii, and candida tropicalis and is loaded to a carrier consisting of one or two of wheat bran, saw dust or straw, and is subjected to freeze drying to obtain the microbial inoculum; and the microbial inoculum is mixed with kitchen garbage, and a fermentation reaction is performed under 50-65 DEG C, so that the kitchen garbage can be reduced by 70% within 6-10 hours, and the content of generated organic matter exceeds 90%. The kitchen garbage biological fermentation microbial inoculum has the advantages that the technical method can efficiently reduce kitchen garbage, the treated substances can be used for soil modification, and the kitchen garbage biological fermentation microbial inoculum has remarkable economic, social and environmental benefits.
Owner:NANKAI UNIV +1

Pseudomonas pseudoalcaligenes disinsection protein gene

The present invention provides a kind of new pesticidal protein gene and the gene coded protein. The pesticidal protein gene is originated from Pseudomonas pseudoalcalligenes, and its coded protein has wide and powerful intoxicating effect on grasshopper and Orthoptera insects, so that the pesticidal protein gene may be used in preventing and controlling pest of crop. The present invention also provides the Pseudomonas pseudoalcalligenes pesticidal protein separating and purifying method, and one method of producing pesticidal transgenic plant.
Owner:SICHUAN UNIV

Efficient degrading microbial inoculum for regenerated papermaking wastewater and preparation method thereof

ActiveCN103087966AStrong concentration adaptabilityEnhanced degradation functionBacteriaTreatment using aerobic processesBiotechnologyActivated sludge
The invention provides an efficient degrading microbial inoculum for regenerated papermaking wastewater, which is characterized in that strains of a compound microbial inoculum are composed of huge bacillus strains, pseudomonas stutzeri strains and pseudomonas alcaligenes strains. In addition, the invention also provides a preparation method and a practical method of the efficient degrading microbial inoculum for regenerated papermaking wastewater. Microorganisms in traditional activated sludge and biological membrane methods are derived from natural bacteria, the period of the adopted natural domestication method is long, and the formation of mature biological treating ponds needs ten days to months; but by using the method, the period can be reduced to 5-20 days through adding a biological enhancer.
Owner:广州市微生物研究所集团股份有限公司

Efficient mixed flora for treating sludge

The invention discloses efficient mixed flora for treating sludge which comprises comamonas testosteroni, pseudomonas alcaligenes, stenotrophomonas rhizophila, pseudomonas pseudoalcaligenes and azospirillum. The mixed flora can effectively decompose the sludge.
Owner:YUNNAN SHENGQING ENVIRONMENTAL PROTECTION TECH CO LTD

Microbial treatment method for domestic garbage

The invention relates to a microbial treatment method for domestic garbage. A biological treatment agent containing bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, pseudomonas pseudoalcaligenes and saccharomyces cerevisiae is added with water and then is subjected to mixed fermentation with the garbage, so that the objective of degrading the garbage is achieved, and the degradation rate is up to 85%. The method is simple in operation and easy to master, moreover, has good effect on degradation of the garbage, and is beneficial for rapid treatment of the garbage and environmental protection.
Owner:SHANGHAI KDN BIOTECH

Preparing method of compound microorganism agent for papermaking wastewater

InactiveCN104830741APurify waterAchieve optimal productionFungiBacteriaWater qualityChrysosporium species
The invention provides a preparing method of a compound microorganism agent for papermaking wastewater. The compound microorganism agent is prepared by mixing Trametes versicolor, Phanerochaete chrysosporium, Bacillus subtilis, Bacillus pumilus, Pseudomonas alcaligenes and cellulase; the cellulase can decompose celluloses in the wastewater into glucose; compound microorganism can also degrade solids, lignin and the like in the wastewater. The compound microorganism agent is free of chemicals, environment friendly, non-toxic and free of secondary pollution, is simple to use, is insusceptible to environmental factors, has stable effect on wastewater treatment and allows the quality of effluent to meet the national standard.
Owner:SHAANXI UNIV OF SCI & TECH

Pseudomonas pseudoalcaligenes and application of pseudomonas pseudoalcaligenes to preparation of sitagliptin intermediate

The invention discloses pseudomonas pseudoalcaligenes and application of the pseudomonas pseudoalcaligenes to preparation of a sitagliptin intermediate. A colony of the pseudomonas pseudoalcaligenes XW-40 is flat and round in surface morphology, smooth and neat in edge, moist, milk white and opaque and is about 1.9mm in diameter. The pseudomonas pseudoalcaligenes is capable of effectively catalyzing 4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro-[1,2,4]triazolo[4,3-a]pyrazine-7(8H)-yl]-1-(2,4,5)-trifluorophenyl)-butan-2-one to be reduced into a sitagliptin chiral intermediate; in optimal reaction conditions, the pseudomonas pseudoalcaligenes can be tolerant to 10 g / L of high substrate concentration while keeps high selectivity, and is weak in substrate inhibition effect, so that high-substrate-concentration biological catalysis can be achieved; the pseudomonas pseudoalcaligenes is recyclable and removes inhibition effect of a substrate and a product on cells as compared with fed-batch, so that catalytic activity of the pseudomonas pseudoalcaligenes is used maximally.
Owner:CHONGQING UNIV OF POSTS & TELECOMM

Composite microbial flocculant and application thereof

The invention relates to the field of water treatment, particularly a composite microbial flocculant and application thereof. The composite microbial inoculant comprises the following deodorizing dominant strains: Saccharomyces (the collection number is CGMCC No.3278), Rhodococcus (the collection number is CGMCC No.3276), and Pseudomonas alcaligenes (the collection number is CGMCC No.3902). When the composite microbial flocculant is applied to the existing aeration-oxygenation technique / microbial restoration technique / artificial wetland restoration technique combined biological restoration treatment process by adding the composite microbial flocculant according to the new development and research technique to the microbial restoration technique, the microbial restoration efficiency is greatly enhanced, and the cracked biologic chain water body is quickly and stably restored, thereby well solving the practical problems.
Owner:李捍东

Decaffeinating microorganism and process of bio-decaffeination of caffeine containing solutions

InactiveUS20040191333A1Simple and safe and efficientLong durationBiocideOrganic chemistryDecaffeinationMicroorganism
The present invention relates to a simple, safe, and efficient process for the complete bio-decaffeination of caffeine-containing solutions using fungus Pseudomonas alcaligenes CFR 1708, a method of isolating pseudomonas alcaligenes CFR 1708 useful for the bio-decaffeination of caffeine-containing solutions, a Pseudomonas alcaligenes strain of accession number CFR 1708, and a decaffeinated solution obtained by aforementioned process.
Owner:COUNCIL OF SCI & IND RES

Pseudomonas compound microbial agent and application thereof in disease resistance, yield increase and quality improvement of angelica sinensis

ActiveCN113403236AHas ACC deaminase activityImprove the immunityBiocidePlant growth regulatorsPseudomonas psychrophilaPseudomonas alcaligenes
The invention belongs to the technical field of microorganisms and biology, and particularly relates to a compound microbial agent of microorganisms and application of the compound microbial agent in disease resistance, yield increase and quality improvement of angelica sinensis. The compound microbial agent is prepared by compounding pseudomonas fluorescens, pseudomonas alcaligenes and pseudomonas psychrophila; the compound microbial agent has ACC deaminase activity, SOD activity, POD activity, NEX neutral xylanase activity, chitinase activity and laccase activity; the growth of medicinal organ roots of the underground part of the angelica sinensis can be promoted, the rhizome diameter, rhizome length, main root length and average single plant weight are increased, the yield is increased, and the appearance character of the commercial angelica sinensis is improved; the content of iconic components such as ferulic acid, Z-ligustilide and senkyunolide A, endogenous jasmonic acid and salicylic acid of the angelica sinensis can be increased, the quality of the angelica sinensis can be remarkably improved, and the compound microbial agent can be used for preparing the special microbial fertilizer for the angelica sinensis; and the compound microbial agent can prevent and treat plant diseases caused by fusarium pathogenic fungi, and can be used for preparing pesticides for preventing and treating plant diseases.
Owner:GANSU ACAD OF SCI INST OF BIOLOGY +1

Composite probiotic agent for improving egg quality of laying hens, preparation method and application

The invention discloses a composite probiotic agent for improving egg quality of laying hens, a preparation method and application. The composite probiotic agent comprises composite probiotics and a carrier, wherein the compound probiotics comprise 4.0-4.5*10<10>CFU / mL of bacillus velezensis, 4.0-5.0*10<10>CFU / mL of lactobacillus plantarum, 1.0-1.5*10<8>CFU / mL of bacteroides faecalis and 1.0-1.5*10<8>CFU / mL of pseudomonas alcaligenes; and the carrier comprises rice chaff powder and starch. Compared with the prior art, the compound probiotic agent disclosed by the invention not only can improvethe intestinal function of the laying hens, but also can improve the color and taste of eggs and improve the egg quality of the laying hens, especially can effectively increase the proportion of unsaturated fatty acid omega-3 / omega-6 in the eggs and improve the nutritional value of the eggs; and therefore, the composite probiotic agent is suitable for being used as feed additives for large-scalebreeding.
Owner:ANHUI AGRICULTURAL UNIVERSITY

Decaffeinating microorganism and process of bio-decaffeination of caffeine containing solutions

InactiveUS7141411B2Simple and safe and efficient processSimple and safe and efficientBiocideOrganic chemistryMicroorganismDecaffeination
The present invention relates to a simple, safe, and efficient process for the complete bio-decaffeination of caffeine-containing solutions using fungus Pseudomonas alcaligenes CFR 1708, a method of isolating pseudomonas alcaligenes CFR 1708 useful for the bio-decaffeination of caffeine-containing solutions, a Pseudomonas alcaligenes strain of accession number CFR 1708, and a decaffeinated solution obtained by aforementioned process.
Owner:COUNCIL OF SCI & IND RES

Complex microbial inoculant for black and odorous water body as well as preparation method and application thereof

ActiveCN109576189APromote decompositionPromote decomposition and effectively inhibit the growth and reproduction of harmful bacteria in waterBacteriaMicroorganism based processesWater qualityParacoccus denitrificans
The invention discloses a complex microbial inoculant for a black and odorous water body as well as a preparation method and application thereof. The complex microbial inoculant is obtained by singlyculturing strains such as pseudomonas borbori, pseudomonas alcalophila, geobacillus thermophilus, paracoccus denitrificans and micrococcus lysodeikticus, performing mixed inoculation and fermentation,and then performing collection. The metabolic function of the strains contained in the complex microbial inoculant has high synergetic complementarity; the thallus reproduction rate is high; the production cost is low; the environment protection is achieved; no pollution exits; the performance is excellent and efficient; the water quality of the black and odorous water body can be effectively improved; the COD, NH3-N, TP, and odor degree and chromaticity of the water body can be effectively reduced; the transparency of the water body is improved.
Owner:合肥市东方美捷分子材料技术有限公司 +1

Microbial compound fungicide for degrading waste gas of rubber plant and preparation method and application thereof

The invention discloses a microbial compound fungicide for degrading waste gas of a rubber plant and a preparation method and application thereof. The compound fungicide comprises, by mass, 5-10 partsof bacillus atrophaeus, 5-10 parts of empedobacter brevis, 2-5 parts of photobacterium beijerinck, 2-5 parts of pseudomonas alcaligenes, 2-5 parts of virgibacillus pantothenticus and 5-10 parts of pseudomonas putida. The microbial compound fungicide can be applied to degradation of the waste gas of the rubber plant, the degradation effect is good, the culture cost is low, the microbial compound fungicide is easy and convenient to use, the VOCs removal rate is 90% or above, and the national emission standard is met.
Owner:青岛软控海科科技有限公司

Microbial agent capable of degrading garbage

The invention relates to the field of microbial technology, and particularly provides a microbial agent capable of degrading garbage. Domestic garbage, especially kitchen waste, can be efficiently degraded through the synergetic promoting effect of bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, pseudomonas pseudoalcaligenes and saccharomyces cerevisiae, the degradation rate can achieve 80%, the living environment can be beneficially improved, and the environmental pollution can be alleviated.
Owner:QINGDAO VLAND BIOTECH GRP

Complex microbial inoculant for petroleum hydrocarbon and benzene series treatment as well as preparation method and application thereof

The invention relates to the field of biotechnology and environmental protection, in particular to a microbial inoculant, and particularly relates to a complex microbial inoculant for petroleum hydrocarbon and benzene series treatment as well as a preparation method and application thereof. The active ingredients of the complex microbial inoculant comprise aspergillus oryzae, streptomyces, pseudomonas putida, pseudomonas alcaligenes, brevibacterium epidermidis and Erwinia; and the complex microbial inoculant is especially suitable for treating soil polluted by petroleum hydrocarbon and benzeneseries. After the complex microbial inoculant disclosed by the invention is added into the soil polluted by the petroleum hydrocarbon and benzene series, indigenous degrading flora can be rapidly propagated, and the remediation process can be rapidly started; in the repairing process, the indigenous degrading bacteria are always in the dominant flora status, and high degradation efficiency is kept for a long time through the synergistic cooperation of the flora in the complex microbial inoculant; and finally, the polluted soil is completely repaired, the degradation rate of petroleum hydrocarbon and benzene series is high, and the removal rate of BTEX and TPH can reach 98% or above.
Owner:BEIJING GUOHUAN TSINGHUA ENVIRONMENT ENG DESIGN & RES INST CO LTD BEIJING CHINA

Denitrifying phosphate-accumulating organism with nitrogen and phosphorus removal functions and applications thereof

ActiveCN103114062BThorough denitrificationHigh denitrification activityBacteriaMicroorganism based processesTherapeutic effectNitrogen gas
The invention relates to a denitrifying phosphate-accumulating organism with nitrogen and phosphorus removal functions and applications thereof. The strain is named as pseudomonas pseudoalcaligenes CL-1, belongs to pseudomonas pseudoalcaligenes, and is preserved in China Center for Type Culture Collection on June 13, 2012, and the preservation numbers is CCTCC NO: M2012225. The strain is gram-positive and belongs to brevibacterium, a bacterial colony is rounded, milk white, convex and glossy, and edges are smooth and transparent. The growth cycle of the organism is long, the nitrogen and phosphorus removal rates in the first 24h are respectively 56.93% and 43.56%, and the nitrogen and phosphorus removal rates in the whole growth cycle are respectively 75.6% and 57.5%. The strain has a complete denitrifying capacity, and can directly reduce inorganic nitrogen such as nitrate nitrogen and nitrite nitrogen and the like to harmless nitrogen gas discharged from water bodies. When the denitrifying phosphate-accumulating organism is used for treating nitrogen and phosphorus containing wastewater, the process is simple, the treatment effect is efficient and stable, and the operating cost is saved, meanwhile, no greenhouse gas such as NO and N2O and the like is produced, therefore, no air pollution is caused.
Owner:SHANDONG UNIV

Complex microbial inoculant for wastewater treatment and preparation method of complex microbial inoculant

The invention discloses a complex microbial inoculant for wastewater treatment and a preparation method thereof.The complex microbial inoculant comprises shewanella putrefaciens, pseudomonas pseudoalcaligenes and modified zeolite.Chitosan is introduced to the zeolite, the adsorption performance of the zeolite is improved, then composite modification is conducted, iron phosphate is introduced to the zeolite, and the adsorption performance of the zeolite is improved; the iron ions can be used as a coagulant and cooperate with chitosan to flocculate and settle ammonia nitrogen substances in sewage, the biological nitrification performance of the composite microbial inoculant is greatly improved, in addition, the iron ions can increase the content of organic polymers in bacterial biofilms, and the colloidal network structure of the organic polymers can aggregate cells together, so that the biological nitrification performance of the composite microbial inoculant is improved. Meanwhile, the biological membrane is adhered to the surface of the zeolite carrier, so that the connectivity between the zeolite carrier and microorganisms is increased, the leakage of the microorganisms is effectively prevented, and the complex microbial inoculant can keep a stable biological nitrification effect.
Owner:江苏利然环保科技有限公司

Pseudo monads pseudoalcaligenes gene promoter

The present invention provides new promoter sequence of Pseudomonas pseudoalcaligenes. The present invention includes gene constituent containing the promoter sequence of the present invention, and the promoter sequence is connected to neomycin phosphotransferase gene sequence encoding the structure gene optionally. The present invention also provides the carrier expressing structure gene encoded product of gene constituent in the present invention and the host cell as well as the cell transformed by the heterologous gene connected to the promoter optionally.
Owner:SICHUAN UNIV

Composite growth promoter for promoting tobacco growth

The invention relates to a composite growth promoter. The microbial agent contains Bacillus subtilis, Bacillus amyloliquefaciens, Bordetella sp., Sinorhizobium sp., Pseudomonas alcaligenes, Nitrobacteriaceae, actinomycete, Sporotrichum thermophile, potassium aspartate and gibberellin. The growth promoter provided by the invention can be used for improving agronomic characters of tobacco such as plant height, stem girth, maximum leaf length, maximum leaf width and effective number of leaves; the growth promoter can also be used for improving the accumulation of amount of dry matters of the tobacco in parts such as roots, stems, leaves and the like; and the composite growth promoter provided by the invention can be used for improving the quality of growth of the tobacco.
Owner:BEIJING SHENGDA LONGTENG TECH DEV
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