Efficient degrading microbial inoculum for regenerated papermaking wastewater and preparation method thereof

A high-efficiency technology for papermaking wastewater, which is applied in the field of high-efficiency degradation bacterial agents for recycled papermaking wastewater and its preparation, can solve the problems of bacteria micelles or biofilms with long cycles, bacteria that have not been artificially domesticated, and cannot achieve degradation effects, etc., to achieve Easy delivery, improved effect, and strong adaptability

Active Publication Date: 2013-05-08
广州市微生物研究所集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the use of natural bacteria for degradation, the cycle of forming bacterial micelles or biofilms is long, and the bacteria have not been artificially domesticated, so the degradation ability is limited. When the concentration of sewage entering the pool is too high, the bacteria often die or cannot be degraded. Effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Preparation method of recycled papermaking wastewater efficiently degrading composite bacterial agent

[0030] S1 strain isolation and screening

[0031]Collect the wastewater from the wastewater treatment tank of the paper mill, add it to three kinds of liquid selective medium, and shake it with a shaker flask for culture, the shaker speed is 200r / min, the culture temperature is 36±1°C, and the culture time is 48h. The cultured bacterial suspension was diluted by the ten-fold serial dilution method, and the last few dilutions were selected and transferred to the solid selective culture plate by the coating method, cultured at 36±1°C for 48 hours, and the single colony on the plate was picked to proliferate After the cultivation, the waste water organic matter degradation experiment was done, and the strain with the highest COD degradation rate was selected as the starting bacteria for the production of the compound bacterial agent.

[0032] Production of S2 ...

Embodiment 2

[0034] The use of embodiment 2 compound bacterial agents

[0035] It can be added at the initial stage of the establishment of the biological pool, and the compound bacterial agent produced in Example 1 can be directly added in an amount of 0.1g / L, and it can cooperate with the natural bacteria to accelerate the formation of bacterial micelles or biofilms; it can also be run in the biological pool During the process, when the influent water quality fluctuates or is polluted by toxic and harmful substances, when the natural bacteria die more, it will be added, and the added amount is 0.01g / L.

Embodiment 3

[0036] Embodiment 3 Comparison of effects

[0037] Through the use of this patent, the domestication period of the biological pool can be shortened from 10 days to several months to 5 to 20 days, and it can be restored by adding bacterial agents when the biological pool encounters external unfavorable factors that cause fluctuations in the microbial system. Normal function of the biological treatment tank. The waste water degraded by this patent can reduce COD from 600mg / L to less than 100mg / L, meeting the discharge standard.

[0038] Prepare 5 biological pools, when the initial COD values ​​are the same, add the compound bacterial agent obtained in Example 1 of the application according to the method of Example 2, one biochemical pool does not add the complex bacterial agent, and the remaining 3 biochemical pools are added separately Add Bacillus megaterium, Pseudomonas stutzeri and Pseudomonas alcaligenes, observe for 20 days, and test 5 biochemical pools respectively at 5 ...

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PUM

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Abstract

The invention provides an efficient degrading microbial inoculum for regenerated papermaking wastewater, which is characterized in that strains of a compound microbial inoculum are composed of huge bacillus strains, pseudomonas stutzeri strains and pseudomonas alcaligenes strains. In addition, the invention also provides a preparation method and a practical method of the efficient degrading microbial inoculum for regenerated papermaking wastewater. Microorganisms in traditional activated sludge and biological membrane methods are derived from natural bacteria, the period of the adopted natural domestication method is long, and the formation of mature biological treating ponds needs ten days to months; but by using the method, the period can be reduced to 5-20 days through adding a biological enhancer.

Description

technical field [0001] The present invention relates to the field of papermaking wastewater treatment, more specifically, to a highly efficient degrading bacterial agent for recycled papermaking wastewater and a preparation method thereof Background technique [0002] The wastewater discharge of my country's paper industry is about 2.4 billion tons per year, second only to the annual discharge of the chemical and steel industries, ranking third in industrial wastewater, accounting for about 10% of the total. The COD emission is more than 3 million tons, accounting for 42% of the total COD emission in the country, and it is a veritable big polluter. [0003] The paper industry can be divided into pulp and paper and recycled paper, which use wood, grass and other plants as raw materials. Recycled papermaking is to use waste paper as a renewable resource for papermaking. Every ton of waste paper recycled can save 3 cubic meters of wood, 100 cubic meters of water, and 600 kW...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/02C02F3/12C12R1/11C12R1/38C02F103/28
CPCY02W10/10
Inventor 杨冠东丁年平钟瑜黎婉园杨础华夏枫耿周卓为石笛江洁芳冼英华郑飞龙
Owner 广州市微生物研究所集团股份有限公司
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